Studies on soluble carbohydrates in yellow lupin (Lupinus luteus L.) seeds of different age

Yellow lupin seeds cv. Juno were stored under laboratory conditions for 2 month, 4, 6 and 8 years. Eighteen soluble carbohydrates were identified in embryonic axes and cotyledons of different age seeds. The concentration of soluble carbohydrates in analysed seeds ranged from 25 to 34% of dry mass. Axes contained more carbohydrates than cotyledons. Stachyose dominated in axes, and verbascose - in cotyledons. Other detected galactosides were: galactinol, galactosyl pinitols and galactosyl chiro-inositols (fagopyritols), but their content was several-fold lower than that of RFOs (in both axes and cotyledons tissues). The concentration of soluble carbohydrates indicated, that sucrose to RFOs mass ratio, or other changes in sugars composition are not indicators of seed storage.

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Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2008.024 ◽

2011 ◽

Vol 77 (3) ◽

pp. 201-205 ◽

Cited By ~ 2

Author(s):

Agnieszka I. Piotrowicz-Cieślak ◽

Dariusz J. Michalczyk ◽

Kamila Górska ◽

Zofia Bulińska-Radomska ◽

Ryszard J. Górecki

Keyword(s):

Seed Coat ◽

Room Temperature ◽

Seed Storage ◽

Lupinus Luteus ◽

Soluble Carbohydrates ◽

Raffinose Family Oligosaccharides ◽

Seed Vigour ◽

Long Term Storage ◽

Different Temperatures ◽

Lupin Seeds

Seed vigour, viability, the contents of soluble carbohydrates, total protein, albumins, and globulins, as well as seed coat structure, were analysed in yellow lupin (Lupinus luteus L.) cv. Iryd seeds stored for 20 years at -14oC, 0oC or at room temperature (approx. +20oC). Seed storage at room temperature reduced viability (to 2%) and increased seed leachate electroconductivity. Determinations of total proteins showed that protein content was significantly reduced in seeds stored at +20oC compared to the other storage regimens. Raffinose family oligosaccharides were the main soluble carbohydrates in seeds stored at 0oC and -14oC, whereas sucrose dominated in seeds stored at room temperature. Scanning electron microscopy (SEM) of seed surface and seed coat sections revealed appearance of an amorphic layer on the surface of seeds stored at room temperature (not observed in other seeds) and distinct shrinking of macrosclereid layer in seeds stored at -14oC. Macrosclereids layer in all seeds was 100 um thick and accounted for 60% of seed coat thickness. The obtained results suggest that for long term storage of lupin seeds at 0oC is the most advisable temperature if both costs of storage and seed storability are considered.

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Soluble sugars and flatulence-producing oligosaccharides in maturing yellow lupin (Lupinus luteus L.) seeds

Seed Science Research ◽

10.1017/s0960258500003524 ◽

1997 ◽

Vol 7 (2) ◽

pp. 185-194 ◽

Cited By ~ 14

Author(s):

Ryszard J. Górecki ◽

Agnieszka Piotrowicz-Cieślak ◽

Ralph L. Obendorf

Keyword(s):

Soluble Sugars ◽

Dry Mass ◽

Fresh Mass ◽

Lupinus Luteus ◽

Yellow Lupin ◽

Seed Germinability ◽

Seed Desiccation ◽

Seed Growth ◽

Lupin Seeds ◽

Mature Seeds

AbstractThe flatulence-producing soluble oligosaccharides are an important component of lupin seeds and were assayed to establish the pattern of their accumulation in relation to germinability during seed development and maturation. Maturing yellow lupin cv. Juno seeds were harvested at 5-day intervals from 15 to 45 days after flowering (DAF). Seed fresh mass increased to a maximum at 35 DAF followed by a decrease when axis and cotyledon tissues changed colour from green to yellow. Maximum seed fresh mass corresponded to the maximum seed size. Seed dry mass continuously increased until 40 DAF. About 75% of mature seed dry mass was in cotyledons, 22% in testa, and 3% in axis. Maximum seed germinability occurred at 45 DAF after maximum seed dry mass and desiccation. Maturing yellow lupin seeds were desiccation tolerant. Mature dry seeds contained 10.9% oligosaccharides and 1.5% sucrose. During seed growth stachyose accumulation preceded accumulation of raffinose and verbascose. The highest rate of oligosaccharide accumulation appeared during seed desiccation and correlated with the acquisition of the ability to germinate. The sucrose:oligosaccharide ratio continuously decreased reaching 0.13 in mature seeds. Accumulation of oligosaccharides in maturing yellow lupin seeds is associated with seed germinability and seed desiccation tolerance.

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Contents of soluble carbohydrates in yellow lupin seeds maturated at various temperatures

Acta Physiologiae Plantarum ◽

10.1007/s11738-006-0031-0 ◽

2006 ◽

Vol 28 (4) ◽

pp. 349-356 ◽

Cited By ~ 3

Author(s):

Agnieszka I. Piotrowicz-Cielak

Keyword(s):

Soluble Carbohydrates ◽

Yellow Lupin ◽

Lupin Seeds

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Adenosine 5′-tetraphosphate phosphohydrolase from yellow lupin seeds: purification to homogeneity and some properties

Biochemical Journal ◽

10.1042/bj3280257 ◽

1997 ◽

Vol 328 (1) ◽

pp. 257-262 ◽

Cited By ~ 10

Author(s):

Andrzej GURANOWSKI ◽

Elżbieta STARZYŃSKA ◽

Paul BROWN ◽

G. Michael BLACKBURN

Keyword(s):

Divalent Cation ◽

Polypeptide Chain ◽

Lupinus Luteus ◽

Nucleoside Triphosphate ◽

Yellow Lupin ◽

Ph Optimum ◽

Single Polypeptide Chain ◽

Lupin Seeds ◽

Single Polypeptide ◽

Hydrolysis Of

Adenosine 5ʹ-tetraphosphate phosphohydrolase (EC 3.6.1.14) has been purified to homogeneity from the meal of yellow lupin (Lupinus luteus) seeds. The enzyme is a single polypeptide chain of 25±1 kDa. It catalyses the hydrolysis of a nucleoside 5ʹ-tetraphosphate to a nucleoside triphosphate and orthophosphate, and hydrolysis of tripolyphosphate but neither pyrophosphate nor tetraphosphate. A divalent cation, Mg2+, Co2+, Ni2+ or Mn2+, is required for these reactions. The pH optimum for hydrolysis of adenosine 5ʹ-tetraphosphate (p4A) is 8.2, Vmax is 21±1.7 μmol/min per mg of protein and the Km for p4A is 3±0.6 μM. At saturating p4A concentrations, the rate constant for the reaction is 8.5±0.7 s-1 [at 30 °C, in 50 mM Hepes/KOH (pH 8.2)/5 mM MgCl2/0.1 mM dithiothreitol]. p4A and guanosine 5ʹ-tetraphosphate are hydrolysed at the same rate. Adenosine 5ʹ-pentaphosphate (p5A) is degraded 1/200 as fast and is converted into ATP and two molecules of orthophosphate, which are liberated sequentially. This contrasts with the cleavage of p5A by the lupin diadenosine tetraphosphate hydrolase (EC 3.6.1.17), which gives ATP and pyrophosphate. Zn2+, F- and Ca2+ ions inhibit the hydrolysis of p4A with I50 values of 0.1, 0.12 and 0.2 mM respectively.

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Shaping of the fungal communities isolated from yellow lupin seeds (Lupinus luteus L.) throughout storage time

Acta Agrobotanica ◽

10.5586/aa.2005.066 ◽

2012 ◽

Vol 58 (2) ◽

pp. 407-416

Author(s):

Bożena Cwalina-Ambroziak ◽

Tomasz P. Kurowski

Keyword(s):

Crop Rotation ◽

Storage Time ◽

Colletotrichum Gloeosporioides ◽

Lupinus Luteus ◽

Yellow Lupin ◽

Storage Duration ◽

Rhizopus Nigricans ◽

Lupin Seeds ◽

Traditional Cultivars ◽

Yellow Lupine

The object of the experiment were seeds of two traditional cultivars of yellow lupin (Juno and Amulet) cultivated in 1999 in two crop-rotation with 20% and 33% yellow lupine contribution. The quantitative and qualitative composition of the fungal community colonizing the seeds were determined in the laboratory conditions after 0.5-, 1.5- and 2.5-year of storage time. In total 1077 fungal colonies were isolated from the lupin seeds. Fungi representing the species of Penicillium - 29.3%, Alternaria alternata - 26.7% and Rhizopus nigricans - 12.7% were isolated most widely. Among the fungi pathogenic to lupin, the species of Colletotrichum gloeosporioides (16.3% isolates) was dominant. The crop rotation with 20% lupin reduced the number of fungal colonies colonizing the seeds including the pathogens from the species of C. gloeosporioides. Seed disinfection decreased the total number of fungal colonies isolated from both cultivars. Higher number of C. gloeosporioides isolates was found in the combination with disinfected seeds. More fungal colonies were obtained from seeds of cv. Amulet than from those of cv. Juno. The storage duration had an effect on the population and the composition of species of fungi isolated from seeds of yellow lupine. With longer storage population of Penicillium spp. and Rhizopus spp. increased, whereas the population of C. gloeosporioides decreased.

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The Loss of Vigour and Sowing Value of Yellow Lupin Seeds (Lupinus luteus L.) as a Result of Mechanical Harvesting

Plant Breeding and Seed Science ◽

10.1515/plass-2016-0005 ◽

2016 ◽

Vol 73 (1) ◽

pp. 53-62

Author(s):

Agnieszka Faligowska ◽

Grażyna Szymańska ◽

Katarzyna Panasiewicz

Keyword(s):

Electrical Conductivity ◽

Seed Quality ◽

Pearson Correlation ◽

Mean Value ◽

Lupinus Luteus ◽

Pearson Correlation Coefficient ◽

Yellow Lupin ◽

Lupin Seeds ◽

The Mean

AbstractThe aim of the study was to determine the effect of mechanical harvest on the seed quality of yellow lupin. Two effects were studied: the cultivar of yellow lupin (the indeterminate cultivar - Mister and the determinate cultivar - Perkoz) and harvest methods: hand-picked plants with manual shelling of seeds as a control and mechanical shelling with a plot harvester. In comparison with manual shelling of seeds, the mechanical harvest reduced the seed germination and increased the number of abnormal seeds both cultivars. Determinate cultivar was more sensitive, because the loss of its quality was higher (germination of 10%) than indeterminate cultivar (6%). Perkoz had also higher electrical conductivity, with the mean value of 34.3 μS × cm−1× g−1. Manual shelled seeds were characterized by a significantly lower leakage of exudates (24.9 μS × cm−1× g−1), and hence, it exhibited greater vigour than mechanical harvested seeds. Most relations of Pearson correlation coefficient between vigour tests and germination were strong or practically functional.

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Chemical and Nutritional Evaluation of Protein-Rich Ingredients Obtained through a Technological Process from Yellow Lupin Seeds (Lupinus luteus)

Plant Foods for Human Nutrition ◽

10.1007/s11130-019-00768-0 ◽

2019 ◽

Vol 74 (4) ◽

pp. 508-517 ◽

Cited By ~ 2

Author(s):

César Burgos-Díaz ◽

Mauricio Opazo-Navarrete ◽

Traudy Wandersleben ◽

Monserrat Soto-Añual ◽

Tamara Barahona ◽

...

Keyword(s):

Technological Process ◽

Lupinus Luteus ◽

Yellow Lupin ◽

Nutritional Evaluation ◽

Lupin Seeds ◽

Protein Rich

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Soluble carbohydrates in desiccation tolerance of yellow lupin seeds during maturation and germination

Seed Science Research ◽

10.1017/s0960258500003445 ◽

1997 ◽

Vol 7 (2) ◽

pp. 107-116 ◽

Cited By ~ 38

Author(s):

R. J. Górecki ◽

A. I. Piotrowicz-Cieślak ◽

L. B. Lahuta ◽

R. L. Obendorf

Keyword(s):

Desiccation Tolerance ◽

Tolerance Test ◽

Soluble Carbohydrates ◽

Raffinose Family Oligosaccharides ◽

Yellow Lupin ◽

Seed Growth ◽

Before And After ◽

Lupin Seeds ◽

Root Tissues ◽

Galactosyl Cyclitols

AbstractMaturing yellow lupin seeds were desiccation tolerant. Glucose, sucrose and cyclitols (mainly D-pinitol, D-chiro-inositol and myo-inositol) were predominant at the early stages of seed growth. Accumulation of the raffinose family oligosaccharides (RFOs) and the galactosyl cyclitols including galactinol, digalactosyl myo-inositol, galactopinitol A, galactopinitol B, trigalactopinitol A, ciceritol, fagopyritol B1 and fagopyritol B2 appeared during seed maturation; their increase correlated with seed germinability after desiccation. The loss of desiccation tolerance after seed germination was also studied. For the desiccation tolerance test, intact seedlings were dried rapidly or slowly followed by rehydration. Soluble carbohydrates were assayed before and after drying. Root tissues were more sensitive to desiccation than hypocotyl tissues and completely lost desiccation tolerance within 36 h of imbibition after both fast and slow-drying treatments. Survival of hypocotyls decreased gradually up to 96 h after imbibition. Loss of RFOs and galactosyl cyclitols in axis tissues preceded visible germination. Loss of desiccation tolerance was accompanied by loss of RFOs and galactosyl cyclitols and an increase in reducing sugars in cotyledon, hypocotyl and radicle tissues. Drying did not induce the accumulation of RFOs and galactosyl cyclitols in seedling tissues.

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Phytotoxicity of Sodium Chloride Towards Common Duckweed (Lemna Minor L.) and Yellow Lupin (Lupinus Luteus L.)

Archives of Environmental Protection ◽

10.2478/aep-2013-0018 ◽

2013 ◽

Vol 39 (2) ◽

pp. 117-128 ◽

Cited By ~ 9

Author(s):

Łukasz Sikorski ◽

Agnieszka I. Piotrowicz -Cieślak ◽

Barbara Adomas

Keyword(s):

Sodium Chloride ◽

Lemna Minor ◽

Stem Elongation ◽

Cell Structure ◽

Chloride Concentration ◽

Lupinus Luteus ◽

Soluble Carbohydrates ◽

Plant Tissues ◽

Yellow Lupin ◽

Leaf Chlorosis

Abstract Salinity has adverse effects on plants and is one of the causes of environment degradation. Plants have developed many defensive mechanisms, protecting them from sodium chloride (NaCl), including accumulation of osmoprotective compounds, which maintain osmotic balance, protect cell structure and enzymes. In the current study, we investigated the effects of salinity resulting from a range of sodium chloride concentrations (from 0 to 400 mM) on the growth of common duckweed (Lemna minor L.) and yellow lupin (Lupinus luteus L.). Increasing concentration of sodium chloride decreased the area of common duckweed leaves. At the highest applied salt concentration, the decrease of leaf area was associated with leaf chlorosis. In yellow lupin, the increasing sodium chloride concentration inhibited root and stem elongation. The highest tested NaCl concentration of 400 mM completely stopped elongation of yellow lupin shoots. The content of cyclitols and soluble carbohydrates in plant tissues was evaluated as well. Cyclitols (D -chiro -inositol and D -pinitol), as well as soluble carbohydrates (glucose, fructose and sucrose) were detected in common duckweed tissues. Yellow lupin seedlings also contained cyclitols - D -pinitol, myo -inositol and D -chiro -inositol - and soluble carbohydrates - glucose, galactose and sucrose. The content of osmoprotectants in plant tissues, especially sucrose and cyclitols, increased with increasing concentration of sodium chloride in the soil. The results indicate that the content of cyclitols and soluble carbohydrates in plant tissues can be an indicator of plant response to salinity stress.

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