scholarly journals Model research of the pig’s microbiome based on “One Health” concept in the light of the shared human and animal health

2021 ◽  
Vol 75 ◽  
pp. 297-303
Author(s):  
Marta Satora ◽  
Anna Rząsa ◽  
Krzysztof Rypuła ◽  
Katarzyna Płoneczka-Janeczko
Keyword(s):  
Health Status ◽  
Animal Health ◽  
Human Microbiome ◽  
Illumina Miseq ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Personal Genome ◽  
Intestinal Microbes ◽  
Sequencing Technologies ◽  
The Impact

The human microbiome in terms of the number of bacteria exceeds the number of cells in the human body. It is defined as an additional “forgotten organ” and plays a key role in maintaining a high health status, which is conditioned by the maintenance of certain proportions and natural relations between bacteria and cells of the host organism. New diagnostic methods can enable profiling not only the human microbiome, but also livestock. An innovative analytical method, which is next generation sequencing (NGS), is increasingly used in the study of the microbiome. Many bacteria are referred to as “uncultivated” or “non-culturable” and metagenomics has played an important role in detecting these bacteria and has contributed to the development of new media for their cultivation. The main application of NGS in microbiology is to replace the conventional characterization of pathogens based on the assessment of morphology, staining properties and metabolic traits with their genome related characteristics. There are several platforms, i.e. “diagnostic tools”, that use a variety of DNA sequencing technologies, among others Ion Torrent Personal Genome Machine (PGM), Pacific Biosciences (PacBio) and Illumina MiSeq. In the case of swine microbiome, studies of the microbiome with the use of modern sequencing technologies seem to be particularly interesting in the aspect of the upcoming, inevitable changes in preventive and therapeutic procedures in animals. Analyses of this type integrate with the concept of the shared human and animal health and enable an in-depth assessment of the impact of specific factors on the population of intestinal microbes and learning how to “form” the composition of the microbiome in order to improve the quality of husbandry and to maintain the pig’s proper health status.

scholarly journals Model research of the pig’s microbiome based on “one health” concept in the light of the shared human and animal health

2020 ◽  
Vol 74 ◽  
pp. 1-10
Author(s):  
Marta Satora ◽  
Anna Rząsa ◽  
Krzysztof Rypuła ◽  
Katarzyna Płoneczka-Janeczko
Keyword(s):  
Health Status ◽  
Animal Health ◽  
Human Microbiome ◽  
Illumina Miseq ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Personal Genome ◽  
Intestinal Microbes ◽  
Sequencing Technologies ◽  
The Impact

The human microbiome in terms of the number of bacteria exceeds the number of cells in the human body. It is defined as an additional "forgotten organ" and plays a key role in maintaining a high health status, which is conditioned by the maintenance of certain proportions and natural relations between bacteria and cells of the host organism. New diagnostic methods can enable profiling not only the human microbiome, but also livestock. An innovative analytical method, which is next generation sequencing (NGS), is increasingly used in the study of the microbiome. Many bacteria are referred to as "uncultivated" or "non-culturable" and metagenomics has played an important role in detecting these bacteria and has contributed to the development of new media for their cultivation. The main application of NGS in microbiology is to replace the conventional characterization of pathogens based on the assessment of morphology, staining properties and metabolic traits with their genome related characteristics. There are several platforms i.e. "diagnostic tools" that use a variety of DNA sequencing technologies, among others Ion Torrent Personal Genome Machine (PGM), Pacific Biosciences (PacBio) and Illumina MiSeq. In the case of swine microbiome, studies of the microbiome with the use of modern sequencing technologies seem to be particularly interesting in the aspect of the upcoming, inevitable changes in preventive and therapeutic procedures in animals. Analyses of this type enable an in-depth assessment of the impact of specific factors on the population of intestinal microbes and learning how to "form" the composition of the microbiome in order to improve the quality of husbandry and to maintain the proper pig’s health status, integrate with the concept of the shared human and animal health.

scholarly journals Tick-borne zoonoses and commonly used diagnostic methods in human and veterinary medicine

2021 ◽  
Author(s):  
Andrea Springer ◽  
Antje Glass ◽  
Julia Probst ◽  
Christina Strube
Keyword(s):  
Veterinary Medicine ◽  
Diagnostic Tests ◽  
One Health ◽  
Animal Health ◽  
Diagnostic Techniques ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Zoonotic Pathogens ◽  
Health Concept ◽  
The One

AbstractAround the world, human health and animal health are closely linked in terms of the One Health concept by ticks acting as vectors for zoonotic pathogens. Animals do not only maintain tick cycles but can either be clinically affected by the same tick-borne pathogens as humans and/or play a role as reservoirs or sentinel pathogen hosts. However, the relevance of different tick-borne diseases (TBDs) may vary in human vs. veterinary medicine, which is consequently reflected by the availability of human vs. veterinary diagnostic tests. Yet, as TBDs gain importance in both fields and rare zoonotic pathogens, such as Babesia spp., are increasingly identified as causes of human disease, a One Health approach regarding development of new diagnostic tools may lead to synergistic benefits. This review gives an overview on zoonotic protozoan, bacterial and viral tick-borne pathogens worldwide, discusses commonly used diagnostic techniques for TBDs, and compares commercial availability of diagnostic tests for humans vs. domestic animals, using Germany as an example, with the aim of highlighting existing gaps and opportunities for collaboration in a One Health framework.

The role of the mussel Mytilus spp. in the transmission of ostreid herpesvirus-1 microVar

Parasitology ◽  
2017 ◽  
Vol 145 (8) ◽  
pp. 1095-1104 ◽  
Author(s):  
A. J. O’ Reilly ◽  
C. Laide ◽  
A Maloy ◽  
S. Hutton ◽  
B. Bookelaar ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Animal Health ◽  
Viral Transmission ◽  
Diagnostic Methods ◽  
The Pacific ◽  
Herpesvirus 1 ◽  
World Organization ◽  
Ostreid Herpesvirus 1 ◽  
The Impact

AbstractThe Pacific oyster Crassostrea gigas contributes significantly to global aquaculture; however, C. gigas culture has been affected by ostreid herpesvirus-1 (OsHV-1) and variants. The dynamics of how the virus maintains itself at culture sites is unclear and the role of carriers, reservoirs or hosts is unknown. Both wild and cultured mussels Mytilus spp. (Mytilus edulis, Mytilus galloprovincialis and hybrids) are commonly found at C. gigas culture sites. The objective of this study was to investigate if Mytilus spp. can harbour the virus and if viral transmission can occur between mussels and oysters. Mytilus spp. living at oyster trestles, 400–500 m higher up the shore from the trestles and up to 26 km at non-culture sites were screened for OsHV-1 and variants by all the World Organization for Animal Health (OIE) recommended diagnostic methods including polymerase chain reaction (PCR), quantitative PCR (qPCR), histology, in situ hybridization and confirmation using direct sequencing. The particular primers that target OsHV-1 and variants, including OsHV-1 microVar (μVar), were used in the PCR and qPCR. OsHV-1 μVar was detected in wild Mytilus spp. at C. gigas culture sites and more significantly the virus was detected in mussels at non-culture sites. Cohabitation of exposed wild mussels and naïve C. gigas resulted in viral transmission after 14 days, under an elevated temperature regime. These results indicate that mussels can harbour OsHV-1 μVar; however, the impact of OsHV-1 μVar on Mytilus spp. requires further investigation.

scholarly journals Bovine Babesiosis in Turkey: Impact, Current Gaps, and Opportunities for Intervention

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1041
Author(s):  
Sezayi Ozubek ◽  
Reginaldo G. Bastos ◽  
Heba F. Alzan ◽  
Abdullah Inci ◽  
Munir Aktas ◽  
...  
Keyword(s):  
Vaccine Development ◽  
Point Of Care ◽  
Animal Health ◽  
Current Control ◽  
Diagnostic Methods ◽  
Control Measures ◽  
Bovine Babesiosis ◽  
Research Programs ◽  
The Impact

Bovine babesiosis is a global tick-borne disease that causes important cattle losses and has potential zoonotic implications. The impact of bovine babesiosis in Turkey remains poorly characterized, but several Babesia spp., including B. bovis, B. bigemina, and B. divergens, among others and competent tick vectors, except Rhipicephalus microplus, have been recently identified in the country. Bovine babesiosis has been reported in all provinces but is more prevalent in central and highly humid areas in low and medium altitude regions of the country housing approximately 70% of the cattle population. Current control measures include acaricides and babesicidal drugs, but not live vaccines. Despite the perceived relevant impact of bovine babesiosis in Turkey, basic research programs focused on developing in vitro cultures of parasites, point-of-care diagnostic methods, vaccine development, “omics” analysis, and gene manipulation techniques of local Babesia strains are scarce. Additionally, no effective and coordinated control efforts managed by a central animal health authority have been established to date. Development of state-of-the-art research programs in bovine babesiosis to address current gaps in knowledge and implementation of long-term plans to control the disease will surely result in important economic, nutritional, and public health benefits for the country and the region.

scholarly journals Impact of Cadmium Intoxication on Health Status, Rumen and Blood Constituents in Egyptian Ossimi Sheep

2021 ◽  
Vol 10 (2) ◽  
pp. 102-106
Keyword(s):  
Drinking Water ◽  
Health Status ◽  
Rumen Fluid ◽  
Animal Health ◽  
Ammonia Nitrogen ◽  
Strong Positive Correlation ◽  
Toxic Heavy Metals ◽  
Animal Blood ◽  
Antioxidant Markers ◽  
The Impact

Toxic heavy metals particularly Cadmium (Cd) have a hazardous impact on animal health and productivity because of their ill-degradability and bio-accumulation for long periods. This study carried out on 52 Ossimi sheep belonging to Giza Governorate, including 12 sheep considered as control (kept in a private farm) and 40 sheep grazed on an area polluted with cadmium. Drinking water, animal blood and rumen fluid samples were collected from all sheep. Complete blood, rumen and serum constituents were analyzed. Iron, copper, zinc, oxidant, and antioxidant markers were evaluated and Cd levels in water, rumen fluid and serum were investigated to show the impact of Cd on those parameters. Physical examination revealed significant disturbance in health status of Cd-exposed sheep. Rumen fluid examination showed significant increase in rumen pH, significant decrease in rumen ammonia-nitrogen, TVFAs, AST, ALT, GGT, Ca and Ph. Blood constituent revealed significant alteration as significant decrease in RBCs count, Hb, PCV, MCHC and TLC as well as impaired hepatic and renal function and significant decrease of antioxidant markers. These alterations associated with strong positive correlation between these altered parameters and cadmium level in drinking water, rumen fluid and blood samples which markedly increased more than permissible limits; these results should be put in consideration in interpretation of affected animals' status and during treatment and control of cadmium exposed sheep cases as well as consumption of such animals' meat and offal is not recommended.

scholarly journals Phenolics and Carbohydrates in Buckwheat Honey Regulate the Human Intestinal Microbiota

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Li Jiang ◽  
Minhao Xie ◽  
Guijie Chen ◽  
Jiangtao Qiao ◽  
Hongcheng Zhang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Benzoic Acid ◽  
Intestinal Microbiota ◽  
High Performance ◽  
Illumina Miseq ◽  
Intestinal Microbes ◽  
Human Intestinal Microbiota ◽  
Health Products ◽  
Almost All ◽  
The Impact

Intestinal microbiota plays an important role in human health. The aim of this paper is to determine the impact of the phenolics and carbohydrate in buckwheat honey on human intestinal microbiota. We investigated the phenolics and carbohydrate compositions of eight buckwheat honey samples using high-performance liquid chromatography and ion chromatography. The human intestinal microbes were cultured in a medium supplemented with eight buckwheat honey samples or the same concentration of fructooligosaccharides. The bacterial 16S rDNA V4 region sequence of DNA extraction was determined by the Illumina MiSeq platform. 12 phenolics and 4 oligosaccharides were identified in almost all buckwheat honey samples, namely, protocatechuic acid, 4-hydroxy benzoic acid, vanillin, gallic acid, p-coumaric acid, benzoic acid, isoferulic acid, methyl syringate, trans,trans-abscisic acid, cis,trans-abscisic acid, ferulic acid, 4-hydroxybenzaldehyde, kestose, isomaltose, isomaltotriose, and panose. Most notably, this is the first study to reveal the presence of 4-hydroxybenzaldehyde in buckwheat honey. 4-Hydroxybenzaldehyde seems to be a land marker of buckwheat honey. Our results indicate that buckwheat honey can provide health benefits to the human gut by selectively supporting the growth of indigenous Bifidobacteria and restraining the pathogenic bacterium in the gut tract. We infer that buckwheat honey may be a type of natural intestinal-health products.

scholarly journals Diagnosis of Chlamydia trachomatis genital infections in the era of genomic medicine

2021 ◽  
Author(s):  
Seema Shetty ◽  
Christina Kouskouti ◽  
Uwe Schoen ◽  
Nikolaos Evangelatos ◽  
Shashidhar Vishwanath ◽  
...  
Keyword(s):  
Low Income ◽  
Patient Management ◽  
Genomic Medicine ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Genital Infections ◽  
Omics Technologies ◽  
Public Health Burden ◽  
Advantages And Disadvantages ◽  
The Impact

Abstract Purpose Chlamydial genital infections constitute significant sexually transmitted infections worldwide. The often asymptomatic status of C. trachomatis (CT) infections leads to an increased burden on human reproductive health, especially in middle- and low-income settings. Early detection and management of these infections could play a decisive role in controlling this public health burden. The objective of this review is to provide an insight into the evolution of diagnostic methods for CT infections through the development of new molecular technologies, emphasizing on -omics’ technologies and their significance as diagnostic tools both for effective patient management and control of disease transmission. Methods Narrative review of the diagnostic methodologies of CT infections and the impact of the introduction of -omics’ technologies on their diagnosis by review of the literature. Results Various methodologies are discussed with respect to working principles, required specifications, advantages, and disadvantages. Implementing the most accurate methods in diagnosis is highlighted as the cornerstone in managing CT infections. Conclusion Diagnostics based on -omics’ technologies are considered to be the most pertinent modalities in CT testing when compared to other available methods. There is a need to modify these effective and accurate diagnostic tools in order to render them more available and feasible in all settings, especially aiming on turning them to rapid point-of-care tests for effective patient management and disease control.

scholarly journals Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique

2021 ◽  
Vol 15 (10) ◽  
pp. e0009803
Author(s):  
Berta Grau-Pujol ◽  
Helena Martí-Soler ◽  
Valdemiro Escola ◽  
Maria Demontis ◽  
Jose Carlos Jamine ◽  
...  
Keyword(s):  
Public Health Problem ◽  
Diagnostic Methods ◽  
World Health ◽  
Diagnostic Tools ◽  
Reference Standard ◽  
Targeted Interventions ◽  
Low Intensity ◽  
Transmission Interruption ◽  
The Impact ◽  
Composite Reference Standard

World Health Organization goals against soil-transmitted helminthiases (STH) are pointing towards seeking their elimination as a public health problem: reducing to less than 2% the proportion of moderate and heavy infections. Some regions are reaching WHO goals, but transmission could rebound if strategies are discontinued without an epidemiological evaluation. For that, sensitive diagnostic methods to detect low intensity infections and localization of ongoing transmission are crucial. In this work, we estimated and compared the STH infection as obtained by different diagnostic methods in a low intensity setting. We conducted a cross-sectional study enrolling 792 participants from a district in Mozambique. Two stool samples from two consecutive days were collected from each participant. Samples were analysed by Telemann, Kato-Katz and qPCR for STH detection. We evaluated diagnostic sensitivity using a composite reference standard. By geostatistical methods, we estimated neighbourhood prevalence of at least one STH infection for each diagnostic method. We used environmental, demographical and socioeconomical indicators to account for any existing spatial heterogeneity in infection. qPCR was the most sensitive technique compared to composite reference standard: 92% (CI: 83%– 97%) for A. lumbricoides, 95% (CI: 88%– 98%) for T. trichiura and 95% (CI: 91%– 97%) for hookworm. qPCR also estimated the highest neighbourhood prevalences for at least one STH infection in a low intensity setting. While 10% of the neighbourhoods showed a prevalence above 20% when estimating with single Kato-Katz from one stool and Telemann from one stool, 86% of the neighbourhoods had a prevalence above 20% when estimating with qPCR. In low intensity settings, STH estimated prevalence of infection may be underestimated if based on Kato-Katz. qPCR diagnosis outperformed the microscopy methods. Thus, implementation of qPCR based predictive maps at STH control and elimination programmes would disclose hidden transmission and facilitate targeted interventions for transmission interruption.

scholarly journals Clinical long-read sequencing of the human mitochondrial genome for mitochondrial disease diagnostics

2019 ◽  
Author(s):  
Elizabeth Wood ◽  
Matthew D Parker ◽  
Mark J Dunning ◽  
Sirisha Hesketh ◽  
Dennis Wang ◽  
...  
Keyword(s):  
Illumina Miseq ◽  
False Positives ◽  
Diagnostic Methods ◽  
Wilcoxon Test ◽  
Single Nucleotide Variants ◽  
Diagnostic Strategies ◽  
Sequencing Technologies ◽  
Large Deletions ◽  
Mtdna Deletions ◽  
Long Read

AbstractPurposeLong-read, third generation, sequencing technologies have the potential to improve current state of the art diagnostic strategies. In order to determine if long-read sequencing technologies are suitable for the diagnosis of mitochondrial disorders due to mitochondrial DNA (mtDNA) variants, particularly large deletions, we compared the performance of Oxford Nanopore Technologies (ONT) MinION to current diagnostic methods.MethodsWe sequenced mtDNA from nine patients with mtDNA deletion disorders and three normal controls with both ONT MinION and Illumina MiSeq. We applied a computational pipeline to estimate the positions of mtDNA deletions in patients, and subsequently validated the breakpoints using Sanger sequencing.ResultsWe were able to detect mtDNA deletions with a MinION workflow, successfully calling the disease causing event in all cases. Sequencing coverage was in most cases significantly more (p=0.03, Wilcoxon test) uniform with MinION than with MiSeq and subsequent correction of MinION reads improved breakpoint accuracy and reduced false positives. Although heteroplasmic single nucleotide variants are detectable, the high number of false positives and false negatives precludes their use in diagnostics at this time.ConclusionThe MinION is becoming an increasingly attractive diagnostic tool due to the reducing cost, increasing accuracy, and the speed at which data can be obtained.

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