scholarly journals Phenolics and Carbohydrates in Buckwheat Honey Regulate the Human Intestinal Microbiota

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Li Jiang ◽  
Minhao Xie ◽  
Guijie Chen ◽  
Jiangtao Qiao ◽  
Hongcheng Zhang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Benzoic Acid ◽  
Intestinal Microbiota ◽  
High Performance ◽  
Illumina Miseq ◽  
Intestinal Microbes ◽  
Human Intestinal Microbiota ◽  
Health Products ◽  
Almost All ◽  
The Impact

Intestinal microbiota plays an important role in human health. The aim of this paper is to determine the impact of the phenolics and carbohydrate in buckwheat honey on human intestinal microbiota. We investigated the phenolics and carbohydrate compositions of eight buckwheat honey samples using high-performance liquid chromatography and ion chromatography. The human intestinal microbes were cultured in a medium supplemented with eight buckwheat honey samples or the same concentration of fructooligosaccharides. The bacterial 16S rDNA V4 region sequence of DNA extraction was determined by the Illumina MiSeq platform. 12 phenolics and 4 oligosaccharides were identified in almost all buckwheat honey samples, namely, protocatechuic acid, 4-hydroxy benzoic acid, vanillin, gallic acid, p-coumaric acid, benzoic acid, isoferulic acid, methyl syringate, trans,trans-abscisic acid, cis,trans-abscisic acid, ferulic acid, 4-hydroxybenzaldehyde, kestose, isomaltose, isomaltotriose, and panose. Most notably, this is the first study to reveal the presence of 4-hydroxybenzaldehyde in buckwheat honey. 4-Hydroxybenzaldehyde seems to be a land marker of buckwheat honey. Our results indicate that buckwheat honey can provide health benefits to the human gut by selectively supporting the growth of indigenous Bifidobacteria and restraining the pathogenic bacterium in the gut tract. We infer that buckwheat honey may be a type of natural intestinal-health products.

scholarly journals Impact of Chronic Tetracycline Exposure on Human Intestinal Microbiota in a Continuous Flow Bioreactor Model

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 886
Author(s):  
Youngbeom Ahn ◽  
Ji Young Jung ◽  
Ohgew Kweon ◽  
Brian T. Veach ◽  
Sangeeta Khare ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Intestinal Microbiota ◽  
Continuous Flow ◽  
Antimicrobial Drug ◽  
Bioreactor Culture ◽  
Analysis Techniques ◽  
Human Intestinal Microbiota ◽  
Traditional Cultures ◽  
The Impact

Studying potential dietary exposure to antimicrobial drug residues via meat and dairy products is essential to ensure human health and consumer safety. When studying how antimicrobial residues in food impact the development of antimicrobial drug resistance and disrupt normal bacteria community structure in the intestine, there are diverse methodological challenges to overcome. In this study, traditional cultures and molecular analysis techniques were used to determine the effects of tetracycline at chronic subinhibitory exposure levels on human intestinal microbiota using an in vitro continuous flow bioreactor. Six bioreactor culture vessels containing human fecal suspensions were maintained at 37 °C for 7 days. After a steady state was achieved, the suspensions were dosed with 0, 0.015, 0.15, 1.5, 15, or 150 µg/mL tetracycline, respectively. Exposure to 150 µg/mL tetracycline resulted in a decrease of total anaerobic bacteria from 1.9 × 107 ± 0.3 × 107 down to 2 × 106 ± 0.8 × 106 CFU/mL. Dose-dependent effects of tetracycline were noted for perturbations of tetB and tetD gene expression and changes in acetate and propionate concentrations. Although no-observed-adverse-effect concentrations differed, depending on the traditional cultures and the molecular analysis techniques used, this in vitro continuous flow bioreactor study contributes to the knowledge base regarding the impact of chronic exposure of tetracycline on human intestinal microbiota.

Synbiotics impact on dominant faecal microbiota and short-chain fatty acids production in sows

2019 ◽  
Vol 366 (13) ◽  
Author(s):  
Katarzyna Śliżewska ◽  
Agnieszka Chlebicz
Keyword(s):  
Fatty Acids ◽  
Intestinal Microbiota ◽  
High Performance ◽  
Anaerobic Bacteria ◽  
Short Chain Fatty Acids ◽  
Feed Additives ◽  
Short Chain ◽  
Control Group ◽  
The Impact ◽  
Chain Fatty Acids

ABSTRACT The aim of this study was to estimate the influence of synbiotics on intestinal microbiota and its metabolism in sows. Three different synbiotics were administered with feed to animals from three experimental groups. Two groups of sows were given commercially available probiotics (BioPlus 2B®, Cylactin® LBC) as forage additives for comparison. The control group of sows was given unmodified fodder. The study was conducted for 48 days (10 days before farrowing, and continued 38 days after) and faeces samples were collected four times. The scope of this work was to designate the dominant microbiota in sows’ faeces. Therefore, the total number of anaerobic bacteria, Bifidobacterium sp., Lactobacillus sp., Bacteroides sp., Clostridium sp., Enterococcus sp., Enterobacteriaceae, Escherichia coli and yeast was determined, using the plate method. Changes in the concentration of lactic acid, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were also determined in correlation with the feed additives administered to the sows using high-performance liquid chromatography analysis (HPLC). Our results allowed us to conclude that synbiotics have a beneficial effect on intestinal microbiota of sows and its metabolism. We observed that the impact of the synbiotics on the microbiota was more significant than the one induced by probiotics.

High-throughput analysis of the impact of antibiotics on the human intestinal microbiota composition

2013 ◽  
Vol 92 (3) ◽  
pp. 387-397 ◽  
Author(s):  
S.E. Ladirat ◽  
H.A. Schols ◽  
A. Nauta ◽  
M.H.C. Schoterman ◽  
B.J.F. Keijser ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
High Throughput ◽  
Microbiota Composition ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Human Intestinal Microbiota ◽  
The Impact

scholarly journals Model research of the pig’s microbiome based on “One Health” concept in the light of the shared human and animal health

2021 ◽  
Vol 75 ◽  
pp. 297-303
Author(s):  
Marta Satora ◽  
Anna Rząsa ◽  
Krzysztof Rypuła ◽  
Katarzyna Płoneczka-Janeczko
Keyword(s):  
Health Status ◽  
Animal Health ◽  
Human Microbiome ◽  
Illumina Miseq ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Personal Genome ◽  
Intestinal Microbes ◽  
Sequencing Technologies ◽  
The Impact

The human microbiome in terms of the number of bacteria exceeds the number of cells in the human body. It is defined as an additional “forgotten organ” and plays a key role in maintaining a high health status, which is conditioned by the maintenance of certain proportions and natural relations between bacteria and cells of the host organism. New diagnostic methods can enable profiling not only the human microbiome, but also livestock. An innovative analytical method, which is next generation sequencing (NGS), is increasingly used in the study of the microbiome. Many bacteria are referred to as “uncultivated” or “non-culturable” and metagenomics has played an important role in detecting these bacteria and has contributed to the development of new media for their cultivation. The main application of NGS in microbiology is to replace the conventional characterization of pathogens based on the assessment of morphology, staining properties and metabolic traits with their genome related characteristics. There are several platforms, i.e. “diagnostic tools”, that use a variety of DNA sequencing technologies, among others Ion Torrent Personal Genome Machine (PGM), Pacific Biosciences (PacBio) and Illumina MiSeq. In the case of swine microbiome, studies of the microbiome with the use of modern sequencing technologies seem to be particularly interesting in the aspect of the upcoming, inevitable changes in preventive and therapeutic procedures in animals. Analyses of this type integrate with the concept of the shared human and animal health and enable an in-depth assessment of the impact of specific factors on the population of intestinal microbes and learning how to “form” the composition of the microbiome in order to improve the quality of husbandry and to maintain the pig’s proper health status.

scholarly journals Dietary Curdlan Enhances Bifidobacteria and Reduces Intestinal Inflammation in Mice

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1305
Author(s):  
Shafaque Rahman ◽  
Mark Davids ◽  
Patricia H. P. van Hamersveld ◽  
Olaf Welting ◽  
Hakim Rahaoui ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Intestinal Inflammation ◽  
Alcaligenes Faecalis ◽  
Food Additive ◽  
Microbial Interactions ◽  
Oral Gavage ◽  
Microbial Composition ◽  
Colonic Inflammation ◽  
Intestinal Microbes ◽  
Human Intestinal Microbiota

β-glucan consumption is known for its beneficial health effects, but the mode of action is unclear. While humans and mice lack the required enzymes to digest β-glucans, certain intestinal microbes can digest β-glucans, triggering gut microbial changes. Curdlan, a particulate β-glucan isolated from Alcaligenes faecalis, is used as a food additive. In this study we determined the effect of curdlan intake in mice on the intestinal microbiota and dextran sodium sulfate (DSS)-induced intestinal inflammation. The effect of curdlan on the human intestinal microbiota was assessed using i-screen, an assay for studying anaerobic microbial interactions. Mice received oral gavage with vehicle or curdlan for 14 days followed by DSS for 7 days. The curdlan-fed group showed reduced weight loss and colonic inflammation compared to the vehicle-fed group. Curdlan intake did not induce general microbiota community changes, although a specific Bifidobacterium, closely related to Bifidobacterium choerinum, was observed to be 10- to 100-fold more prevalent in the curdlan-fed group under control and colitis conditions, respectively. When tested in i-screen, curdlan induced a global change in the microbial composition of the healthy intestinal microbiota from a human. Overall, these results suggest that dietary curdlan induces microbiota changes that could reduce intestinal inflammation.

scholarly journals Synbiotics impact on dominant faecal microbiota and short-chain fatty acids production in sows

2019 ◽  
Vol 366 (Supplement_1) ◽  
pp. i133-i146
Author(s):  
Katarzyna Śliżewska ◽  
Agnieszka Chlebicz
Keyword(s):  
Fatty Acids ◽  
Intestinal Microbiota ◽  
High Performance ◽  
Anaerobic Bacteria ◽  
Short Chain Fatty Acids ◽  
Feed Additives ◽  
Short Chain ◽  
Control Group ◽  
The Impact ◽  
Chain Fatty Acids

ABSTRACT The aim of this study was to estimate the influence of synbiotics on intestinal microbiota and its metabolism in sows. Three different synbiotics were administered with feed to animals from three experimental groups. Two groups of sows were given commercially available probiotics (BioPlus 2B®, Cylactin® LBC) as forage additives for comparison. The control group of sows was given unmodified fodder. The study was conducted for 48 days (10 days before farrowing, and continued 38 days after) and faeces samples were collected four times. The scope of this work was to designate the dominant microbiota in sows’ faeces. Therefore, the total number of anaerobic bacteria, Bifidobacterium sp., Lactobacillus sp., Bacteroides sp., Clostridium sp., Enterococcus sp., Enterobacteriaceae, Escherichia coli and yeast was determined, using the plate method. Changes in the concentration of lactic acid, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were also determined in correlation with the feed additives administered to the sows using high-performance liquid chromatography analysis (HPLC). Our results allowed us to conclude that synbiotics have a beneficial effect on intestinal microbiota of sows and its metabolism. We observed that the impact of the synbiotics on the microbiota was more significant than the one induced by probiotics.

scholarly journals Fecal microbiota and metabolome characteristics in cirrhotic patients accompanied with hepatic encephalopathy

2019 ◽  
Author(s):  
xiao wei ◽  
Jing Yuan
Keyword(s):  
Hepatic Encephalopathy ◽  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
High Performance ◽  
High Throughput Sequencing ◽  
Paired Comparison ◽  
Illumina Miseq ◽  
Metabolic Phenotype ◽  
Metabolome Analysis ◽  
Cirrhotic Patients

Abstract Background Gut microbiota is considered as an important endocrine organ and has remarkable influence on the metabolic phenotype of host, with extensive participation in the cometabolism with host. Significant changes occurred in intestinal microenvironment of cirrhotic patients under the influence of gut-liver axis. Hepatic encephalopathy is one of the common complications of cirrhosis. Methods In this study, four patients of cirrhosis accompanied with hepatic encephalopathy (CHE) were enrolled, with their healthy relatives as the controls, and paired comparison analysis was carried out. Fresh fecal samples were collected from participants, and subjected to Illumina MiSeq high throughput sequencing of 16S rRNA regions, and metabolome analysis was carried out using high-performance liquid phase chromatography and gas chromatography coupled with tandem mass spectrometry (HPLC-GC/MS-MS). Results Both the intestinal microbiota community and metabolome analysis showed distinct differences between CHE patients and the normal. CHE patients contained a remarkably decreased abundance of Bacteroidetes, whereas increased abundances of Firmicutes and Proteobacteria compared with the normal. Metabolome analysis revealed significant changes in metabolites constituents for CHE patients, showing distinct higher concentrations of amine, alanine, glutamic acid, ornithine, and tyrosine, with lower concentrations of cholesterol, myo-inositol, serine, suggesting the metabolic balance of intestinal microbiota was disrupted. The initial equilibrium state of gut microbiota community and metabolism was broken due to CHE. Conclusions Our study illustrated the intestinal microenvironment dysbiosis and dysfunction characteristics, which will be expected to provide guiding significance for intestinal microecological therapy and improvement of patients prognosis clinically.

scholarly journals Model research of the pig’s microbiome based on “one health” concept in the light of the shared human and animal health

2020 ◽  
Vol 74 ◽  
pp. 1-10
Author(s):  
Marta Satora ◽  
Anna Rząsa ◽  
Krzysztof Rypuła ◽  
Katarzyna Płoneczka-Janeczko
Keyword(s):  
Health Status ◽  
Animal Health ◽  
Human Microbiome ◽  
Illumina Miseq ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Personal Genome ◽  
Intestinal Microbes ◽  
Sequencing Technologies ◽  
The Impact

The human microbiome in terms of the number of bacteria exceeds the number of cells in the human body. It is defined as an additional "forgotten organ" and plays a key role in maintaining a high health status, which is conditioned by the maintenance of certain proportions and natural relations between bacteria and cells of the host organism. New diagnostic methods can enable profiling not only the human microbiome, but also livestock. An innovative analytical method, which is next generation sequencing (NGS), is increasingly used in the study of the microbiome. Many bacteria are referred to as "uncultivated" or "non-culturable" and metagenomics has played an important role in detecting these bacteria and has contributed to the development of new media for their cultivation. The main application of NGS in microbiology is to replace the conventional characterization of pathogens based on the assessment of morphology, staining properties and metabolic traits with their genome related characteristics. There are several platforms i.e. "diagnostic tools" that use a variety of DNA sequencing technologies, among others Ion Torrent Personal Genome Machine (PGM), Pacific Biosciences (PacBio) and Illumina MiSeq. In the case of swine microbiome, studies of the microbiome with the use of modern sequencing technologies seem to be particularly interesting in the aspect of the upcoming, inevitable changes in preventive and therapeutic procedures in animals. Analyses of this type enable an in-depth assessment of the impact of specific factors on the population of intestinal microbes and learning how to "form" the composition of the microbiome in order to improve the quality of husbandry and to maintain the proper pig’s health status, integrate with the concept of the shared human and animal health.

scholarly journals The Host-Specific Intestinal Microbiota Composition Impacts Campylobacter coli Infection in a Clinical Mouse Model of Campylobacteriosis

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 804
Author(s):  
Markus M. Heimesaat ◽  
Claudia Genger ◽  
Sigri Kløve ◽  
Dennis Weschka ◽  
Soraya Mousavi ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Molecular Mechanisms ◽  
Immune Cell ◽  
Inflammatory Responses ◽  
Fecal Microbiota Transplantation ◽  
Campylobacter Coli ◽  
Microbiota Composition ◽  
Mesenteric Lymph Nodes ◽  
Human Intestinal Microbiota ◽  
The Impact

Human Campylobacter-infections are progressively rising globally. However, the molecular mechanisms underlying C. coli–host interactions are incompletely understood. In this study, we surveyed the impact of the host-specific intestinal microbiota composition during peroral C. coli infection applying an established murine campylobacteriosis model. Therefore, microbiota-depleted IL-10−/− mice were subjected to peroral fecal microbiota transplantation from murine versus human donors and infected with C. coli one week later by gavage. Irrespective of the microbiota, C. coli stably colonized the murine gastrointestinal tract until day 21 post-infection. Throughout the survey, C. coli-infected mice with a human intestinal microbiota displayed more frequently fecal blood as their murine counterparts. Intestinal inflammatory sequelae of C. coli-infection could exclusively be observed in mice with a human intestinal microbiota, as indicated by increased colonic numbers of apoptotic epithelial cells and innate as well as adaptive immune cell subsets, which were accompanied by more pronounced pro-inflammatory cytokine secretion in the colon and mesenteric lymph nodes versus mock controls. However, in extra-intestinal, including systemic compartments, pro-inflammatory responses upon pathogen challenge could be assessed in mice with either microbiota. In conclusion, the host-specific intestinal microbiota composition has a profound effect on intestinal and systemic pro-inflammatory immune responses during C. coli infection.

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