scholarly journals The Effect of Endurance Training Along with Cadmium Consumption on Bcl-2 and Bax Gene Expressions in Heart Tissue of Rats

2019 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Hadis Ghajari ◽  
Seyed Ali Hosseini ◽  
Sirous Farsi
Keyword(s):  
Endurance Training ◽  
Heart Tissue ◽  
Gene Expressions ◽  
Bax Gene

The Effects of Ligustrazine on the Expression of Bax Protein and Cytochrome C Following Focal Cerebral Ischemia/Reperfusion Injury in Rats

2013 ◽  
Vol 641-642 ◽  
pp. 824-827
Author(s):  
Qiu Zhen Zhao ◽  
Hua Liu ◽  
Hua Guang Li
Keyword(s):  
Neuronal Apoptosis ◽  
Ischemia Reperfusion Injury ◽  
Focal Cerebral Ischemia ◽  
Ischemia Reperfusion ◽  
Gene Expressions ◽  
Bax Protein ◽  
Cerebral Ischemia Reperfusion Injury ◽  
Hippocampus Neuron ◽  
Bax Gene

AIMS: To explore gene expressions of Bax and changes of cytC releasing in the CIR hippocampus neuron; to observe the effect on CIR with ligustrazine. METHODS: According to Bannisters, we made ischemia and reperfusion models of rat, and then using ligustrazine to cure; we observed the expression changes of Bax gene in hippocampus neurons by nucleic acid in situ hybridization, detecting cytC release through Western blotting. RESULTS: Ligustrazine protect neuron by reducing neuronal apoptosis induced by CIR, and inhibiting release of cytc by releasing.

scholarly journals Activation of Invariant Natural Killer T Cells Further Ameliorates Post-Infarct Cardiac Remodeling in Mice With MSC Transplantation

2021 ◽  
Author(s):  
Wentao Li ◽  
YI HUANG ◽  
CHUANYU GAO ◽  
ZHONGYU ZHU ◽  
GUOYOU DAI
Keyword(s):  
Ventricular Remodeling ◽  
Interstitial Fibrosis ◽  
Heart Tissue ◽  
Tunel Staining ◽  
Complete Medium ◽  
Inkt Cells ◽  
Gene Expressions ◽  
Qrt Pcr ◽  
Killer T Cells ◽  
Invariant Natural Killer

Abstract Background：The purpose of this study was to examine the effect of MSCs on the infiltration of iNKT cells and further observe whether the activation of iNKT cells could assist the therapeutic action of MSCs on ventricular remodeling.Methods：Mice with MI were sacrificed at day 7, 14, 28 and hearts were excised. qRT-PCR for Vα14Jα18 (a special marker of iNKT cell for C57BL/6) was carried out. 25μL serum-free medium(Me)、complete medium with 1×106 MSCs or without MSCs were respectively injected into myocardium in five points. After 7, 14, 28 days, qRT-PCR for Vα14Jα18 was respectively preformed. Gene expressions of Vα14Jα18 in PLV with the injection of cMe alone and cMe with PGE2, L-NMMA, indomethacin, anti-TGF at day 7 were also analyzed. Then, the experiment was performed in five groups: MI+Me, MI+MSCs, MI+MSCs+indome, MI+α-GC and MI+MSCs+ α-GC. After 28 days, heart tissue fibrosis was accessed by Masson trichrome dyeing and apoptosis was evaluated by TUNEL staining and western blotting for caspase-3 protein.Results：The iNKT cells infiltrating into the PLV was significantly increased at day 7 after MI (1.72-fold changes from baseline, P<0.05), but almost returned to baseline level at day 14 and 28. Compared to MI+Me group(1.76±0.20-fold), iNKT cells infiltration was significantly suppressed at day 7 in MI+MSCs (1.25±0.29-fold, P<0.05) and MI+cMe groups (1.19±0.25-fold, P<0.05). In MI+cMe+indome and MI+cMe+PGE2 group, the changes of iNKT cells infiltration were respectively 1.74- and 1.04-fold (vs 1.20-fold in MI+cMe group, P<0.05). Combined MSCs transplantation with iNKT cells activation, myocyte apoptosis and interstitial fibrosis in PLV were both significantly attenuated. Conclusions:In the infarcted mouse model, MSCs suppressed the infiltration of iNKT cells by secreting PGE2. Activating iNKT cells could assist the therapeutic effect of MSCs on ventricular remodeling, with attenuated apoptosis and interstitial fibrosis.

scholarly journals Effect of 8 Weeks of Incremental Endurance Training on Antioxidant Enzymes and Total Antioxidant Status of Cardiac Tissue in Experimental Diabetic Rats

2020 ◽  
Author(s):  
Eftekhar Mohammadi ◽  
Fatemeh Nikseresht
Keyword(s):  
Antioxidant Enzymes ◽  
Endurance Training ◽  
Antioxidant Status ◽  
Cardiac Tissue ◽  
Total Antioxidant Status ◽  
Diabetic Control ◽  
Heart Tissue ◽  
Diabetic Mice ◽  
Total Antioxidant ◽  
Healthy Control

Introduction: Evidence suggests increased oxidative stress and improved total antioxidant status resulting from regular exercise in the heart tissue of diabetic patients. The aim of this study was to investigate the effect of 8 weeks of increasing endurance training on the levels of antioxidant enzymes and the total antioxidant status of the cardiac tissue of diabetic mice. Methods: In this experimental study, 24 Wistar male rats (aged 10 weeks and weighing 256±11.8 g) were divided into 4 groups of 6.The training program lasted for 8 weeks with increasing endurance training. It was measured 48 hours after the completion of the protocol levels of antioxidant enzymes (catalase, glutathione, and glutathione peroxidase) and the total antioxidant status of rat heart tissue. One-way analysis of variance test was used for intergroup comparisons and Pearson test was used to investigate the relationship between indicators. Results: The results showed a significant difference in total antioxidant status, catalase and glutathione (P = 0.001) and were not significant in glutathione peroxidase (P = 0.240). Moreover, a follow-up test showed a significant increase in total antioxidant status and catalase (healthy exercise, healthy control, and diabetic exercise compared to diabetic control), Glutathione (healthy exercise (P = 0.001) and healthy control (P = 0.049) compared to diabetic control); (Healthy exercise (P = 0.003) compared to diabetic exercise. Finally, some correlations between antioxidant enzymes and total antioxidant status were significant. Conclusion: According to research results, increasing endurance training appears to increase levels of antioxidant enzymes and improve total antioxidant status, thereby it reduces oxidative stress in the heart tissue of diabetic mice.

scholarly journals Zerumbone-Loaded Nanostructured Lipid Carrier Induces Apoptosis of Canine Mammary Adenocarcinoma Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-18 ◽  
Author(s):  
Jia Ning Foong ◽  
Gayathri Thevi Selvarajah ◽  
Abdullah Rasedee ◽  
Heshu Sulaiman Rahman ◽  
Chee Wun How ◽  
...  
Keyword(s):  
Cell Death ◽  
Mammary Gland ◽  
Mammary Adenocarcinoma ◽  
Nanostructured Lipid Carrier ◽  
Mammary Gland Tumor ◽  
Gland Tumor ◽  
Gene Expressions ◽  
Significant Difference ◽  
Bax Gene ◽  
Canine Mammary Gland

Canine mammary gland tumor (CMT) is the most common tumor in intact female dog. Zerumbone (ZER) has promising anticancer properties, but plagued with poor water solubility, poor absorption, bioavailability, and delivery to target tissues. To solubilize, ZER was loaded into nanostructured lipid carrier (NLC) to produce ZER-loaded NLC (ZER-NLC). The objectives of this study were to determine the antiproliferative effect and the mode of cell death induced by ZER-NLC and ZER on a canine mammary gland tumor (CMT) adenocarcinoma primary cell line. There was no significant difference (p>0.05) between ZER-NLC and ZER treatments in the inhibition of CMT cell proliferation; thus, the loading of ZER into NLC did not compromise the cytotoxic effect of ZER. Microscopically, ZER-NLC- and ZER-treated CMT cells showed apoptotic cell morphology. ZER-NLC and ZER treatments significantly downregulated the antiapoptotic Bcl-2 and upregulated the proapoptotic Bax gene expressions in CMT cells. Both ZER-NLC and ZER-treated CMT cells showed significant (p<0.0001) increases in caspase-8, -9, and -3/7 protein activities. In conclusion, ZER-NLC induced CMT cell death via regulation of Bcl-2 and Bax gene expressions and caspase activations, indicating the involvement of both the intrinsic and extrinsic pathways of apoptosis. This study provided evidences for the potential of ZER-NLC as an anticanine mammary gland adenocarcinoma chemotherapy.

scholarly journals Effect of Endurance Training with Coriander Seed Consumption on Caspase-3 and Cytochrome-C in the Heart Tissue of H2O2-Poisoned Rats

2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Zahra Mardani ◽  
Seyed Ali Hosseini ◽  
Hassan Matinhomaee ◽  
Saleh Rahmati-Ahmadabad
Keyword(s):  
Cytochrome C ◽  
Endurance Training ◽  
Caspase 3 ◽  
Heart Tissue ◽  
Coriander Seed

scholarly journals Effect of Eight Weeks of Endurance Training and Stevia Supplemention on Atrial Natriuretic Peptide and β-Myosin Heavy Chain Expression Levels in Heart Tissue of Rats With Type 1 Diabetes

2020 ◽  
Vol 24 (4) ◽  
pp. 308-319
Author(s):  
Kobra Soleymani ◽  
◽  
Asieh Abbassi Daloii ◽  
Ali Reza Barari ◽  
Ayoub Saeidi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endurance Training ◽  
Cardiac Tissue ◽  
Diabetic Control ◽  
Heart Tissue ◽  
Control Group ◽  
Expression Levels ◽  
Diabetic Control Group ◽  
Healthy Control

Background: The effects of exercise and stevia extract on diabetes-related indicators have been already reported, but their cardiac benefits on Type 1 Diabetes (T1D) are unclear. Objective: This study aims to evaluate the effect of eight weeks of endurance training and stevia supplementation on gene expression levels of Atrial Natriuretic Peptide (ANP) and β-Myosin Heavy Chain (β-MHC) in the heart tissue of T1D rats. Methods: In this experimental study, 25 rats with the average weight of 250-300 g were divided into five groups; healthy control, diabetic control, diabetic+supplementation, diabetic+training, and diabetic+training+supplementation. T1D was induced by intraperitoneal injection of streptozotocin (55 mg/kg/ body weight). Endurance training was performed 5 days a week at a speed of 20-30 meters per minute on a surface with a zero slope for 8 weeks. Stevia was gavaged in a dose of 250 mg/kg/body weight. Rats were slaughtered 48 hours after the last training session. Cardiac tissue was used to measure the parameters. The gene expression of ANP and b-MHC in cardiac tissue was measured by real-time Polymerase Chain Reaction (PCR) method. Data were analyzed by using one-way ANOVA and Bonferroni post hoc test. Findings: The gene expression levels of ANP and β-MHC were significantly higher in the diabetic control group compared to the healthy control group (P=0.001), and significantly lower in the diabetic+training and diabetic+training+supplementation groups compared to the diabetic control group (P=0.001). Conclusion: Endurance training and stevia supplementation can have beneficial effects on the heart of T1D rats.

Effects of Antioxidant Supplementation and Exercise Training on Erythrocyte Antioxidant Enzymes

2006 ◽  
Vol 76 (5) ◽  
pp. 324-331 ◽  
Author(s):  
Marsh ◽  
Laursen ◽  
Coombes
Keyword(s):  
Antioxidant Enzymes ◽  
Exercise Training ◽  
Endurance Training ◽  
Young Male ◽  
Antioxidant Defenses ◽  
Antioxidant Enzyme Activities ◽  
Antioxidant Supplementation ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
Antioxidant Supplements

Erythrocytes transport oxygen to tissues and exercise-induced oxidative stress increases erythrocyte damage and turnover. Increased use of antioxidant supplements may alter protective erythrocyte antioxidant mechanisms during training. Aim of study: To examine the effects of antioxidant supplementation (α-lipoic acid and α-tocopherol) and/or endurance training on the antioxidant defenses of erythrocytes. Methods: Young male Wistar rats were assigned to (1) sedentary; (2) sedentary and antioxidant-supplemented; (3) endurance-trained; or (4) endurance-trained and antioxidant-supplemented groups for 14 weeks. Erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activities, and plasma malondialdehyde (MDA) were then measured. Results: Antioxidant supplementation had no significant effect (p > 0.05) on activities of antioxidant enzymes in sedentary animals. Similarly, endurance training alone also had no effect (p > 0.05). GPX (125.9 ± 2.8 vs. 121.5 ± 3.0 U.gHb–1, p < 0.05) and CAT (6.1 ± 0.2 vs. 5.6 ± 0.2 U.mgHb–1, p < 0.05) activities were increased in supplemented trained animals compared to non-supplemented sedentary animals whereas SOD (61.8 ± 4.3 vs. 52.0 ± 5.2 U.mgHb–1, p < 0.05) activity was decreased. Plasma MDA was not different among groups (p > 0.05). Conclusions: In a rat model, the combination of exercise training and antioxidant supplementation increased antioxidant enzyme activities (GPX, CAT) compared with each individual intervention.

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