scholarly journals Perfil de resistência de microrganismos isolados em fones de ouvidos de alunos de uma instituição de ensino superior de Juazeiro do Norte-CE

2021 ◽  
Vol 47 (1) ◽  
Author(s):  
Felipe Raimundo Bezerra Barros ◽  
Maria de Fátima Guedes Monteiro ◽  
Dárcio Luiz de Sousa Júnior ◽  
Pedro Everson Alexandre de Aquino ◽  
Cícero Roberto Nascimento Saraiva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Enterobacter Aerogenes ◽  
Citrobacter Freundii ◽  
Candida Sp

O objetivo do presente estudo foi avaliar o perfil de resistência de microrganismos isolados em fones de ouvidos de alunos de uma instituição de ensino superior (IES) de Juazeiro do Norte-CE. Foram adquiridas 40 amostras aleatoriamente com os discentes da IES e acondicionadas em tubos contendo solução salina, essas amostras foram levadas para o laboratório de microbiologia do Centro Universitário Dr. Leão Sampaio, onde foram abertos e inoculados em meio Brain Hair Infusion (BHI), onde foram incubados em estufa bacteriológica a 37 ºC. Após 24 horas foi realizado o semeio nos meios de cultura (Agar Manitol Salgado, Agar Sangue e Agar Eosina Azul de Metileno - EMB), posteriormente foram realizadas as provas bioquímicas para confirmação das espécies, por fim realizou-se o antibiograma  utilizando os  discos de Amicacina, Gentamicina, Ofloxacina, Oxacilina, Norfloxacina, Ciprofloxacina e Penicilina G. Foi observado que 100 % das amostras encontravam-se contaminadas por um ou mais tipos de microrganismos (12,5 % de Streptococcus sp, 70 % de Staphylococcus aureus, Staphylococcus coagulase negativa 30%, Citrobacter freundii 7,5 %, Enterobacter aerogenes 2,5 % e Cândida sp. 35 %). A partir do antibiograma foi possível observar resistência das cepas para os antibióticos Penicilina G e Oxacilina. Baseado nos resultados obtidos é necessário que os discentes tenham um cuidado maior com os fones de ouvidos uma vez que, eles mal higienizados podem aglomerar diversos tipos de bactérias resistentes e podem causar infecções no canal auditivo.

scholarly journals Fatores de risco e perfil do uso de antimicrobianos entre pacientes com infecção no trato urinário em uma unidade de terapia intensiva

2021 ◽  
Vol 10 (3) ◽  
pp. e43910313516
Author(s):  
Lucas Harassim ◽  
Olibio Lopes Fiebig da Silva ◽  
Luiz Felipe Soares Pinheiro ◽  
Elber José Assaiante dos Santos ◽  
Cláudio Daniel Cerdeira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Oxytoca ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Citrobacter Freundii ◽  
Providencia Rettgeri ◽  
E Coli ◽  
Terapia Intensiva

A Infecção no Trato Urinário (ITU) acometendo pacientes em Unidades de Terapia Intensiva (UTIs) é uma realidade preocupante, agravada pelo uso irracional de antimicrobianos e a alarmante multirresistência em microrganismos. Nós avaliamos o nível de assertividade quanto ao uso de antimicrobianos durante à antibioticoterapia empírica (ATE), em pacientes diagnosticados com ITU, comparando tal tratamento farmacológico empírico e o realizado após o antibiograma (antibioticoterapia direcionada), além disto, estimamos a prevalência dos agentes etiológicos e analisamos os fatores de risco associados. Este é um estudo observacional e transversal, realizado em 2015, no qual foram avaliados pacientes de ambos os sexos e todas as idades apresentando ITU e submetidos à antibioticoterapia, internados em uma UTI de um hospital no sul de Minas Gerais, Brasil. Dos 49 pacientes avaliados (28 mulheres [M] e 21 homens [H]), a média de idade foi 55±19 anos (IC(95) 49-61) e a faixa etária ≥70 anos foi a predominante. Quatorze diferentes microrganismos foram causadores de ITUs, sendo que 28,3% (IC(95%) 16,2-40,4) dos isolados clínicos tiveram Escherichia coli como o agente etiológico (33,3% H e 28,6% M); 18,9% (IC(95%) 8,3-29,4) Acinetobacter baumannii (33,3% H e 10,7% M); 15,1% (IC(95%) 5,5-24,7) Klebsiella pneumoniae (19% H e 14,3% M); 11,3% Pseudomonas aeruginosa (9,5% H e 14,3% M); 5,7% Enterobacter aerogenes (14,3% H); 3,8% Klebsiella oxytoca; 3,8% Staphylococcus aureus (7,1% M); e 1,9% para cada um dos seguintes microrganismos: Enterococcus faecalis (4,8% H); Proteus mirabilis (3,6% M); Enterobacter cloacae (3,6% M); Providencia rettgeri (4,8% H); Citrobacter koseri (3,6% M); Citrobacter freundii (3,6% M); e Fungos leveduriformes (4,8% H). As prevalências de ITUs causadas por A. baumannii e P. aeruginosa foram influenciadas pelo sexo (χ² com p<0,001). No sexo masculino, houve correlações positivas “substanciais” entre o aumento da idade (em anos) e a prevalência de ITU causada por E. coli (r = 0,69) ou entre idades menos avançadas e a prevalência de ITU causada por A. baumannii (r = -0,7). No sexo feminino, houve uma correlação positiva “extremamente forte” entre o aumento da idade e a prevalência de ITU causada por E. coli (r = 0,94; IC(95) 0,66-0,99; p<0,0014). Os antibióticos mais utilizados de forma empírica (ATE) foram: Ciprofloxacina (14,3% IC(95%) 4,7-24,1), Cefepima (14,3%) e Vancomicina (10%), e após o antibiograma (antibioticoterapia direcionada): Ceftazidima (16,3% IC(95%) 6-26,7), Ciprofloxacina (14,3% IC(95%) 4,5-24,1), Polimixina B (10,2%), Imipenem (10,2%) e Ampicilina + Sulbac. (8,2%). Em 20% dos casos, as terapias empíricas (ATE) foram consideradas “inapropriadas/não acertadas”. Contudo, também devemos ter ciência da necessidade clínica e quanto ao imediatismo para o tratamento de uma ITU em UTI, uma vez que a doença pode ser fatal se uma terapia não for instituída, portanto, nós aconselhamos avaliações mais minuciosas, tanto da racionalidade do uso de antibióticos, quanto dos fatores de risco para o desenvolvimento de ITUs em UTIs.

scholarly journals Contaminación con bacterias patógenas de estetoscopios del personal médico en un hospital de nivel III en Lima, Perú

2016 ◽  
Vol 27 (2) ◽  
pp. 83 ◽  
Author(s):  
José Enrique Oliva-Menacho ◽  
Marco Antonio García-Hjarles ◽  
José Arturo Oliva-Candela ◽  
Hugo Saturnino De la Cruz-Roca
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Enterobacter Aerogenes ◽  
Macconkey Agar ◽  
Acinetobacter Spp ◽  
Staphylococcus Spp ◽  
Hospital General

Objetivos: Determinar el grado de contaminación bacteriana con bacterias patógenas de los estetoscopios del personal médico en un hospital general de Lima, Perú. Material y métodos: Estudio de tipo observacional, descriptivo y transversal, realizado en el Hospital Nacional Arzobispo Loayza, entre los meses de enero y juniodel 2013. Se estudiaron 124 muestras de estetoscopios del personal médico en las siguientes áreas: UCI 20; neonatología 13; quemados 3; medicina 52; emergencia 36. Se recolectaron las muestras con hisopos humedecidos, en condiciones estériles (En presencia de un mechero de vidrio para alcohol) y luego fueron introducidos en tuboscon preparado de caldo BHI (Infusión cerebro corazón) para ser incubados por 24 horas a 37°C; se cultivó en Agar sangre, Agar MacConkey, Agar manitol y Agar cetrimidepara su posterior determinación de bacterias patógenas por procedimientos bioquímicos ,luego se identificó la susceptibilidad bacteriana con la técnica de Kirby- Bauer. Resultados: De los 124 estetoscopios estudiados; 114 (91,9%) estuvieron contaminados; se aislaron 123 cepasbacterianas: Staphylococcus spp coagulasa negativa 106(86,1%), Staphylococcus aureus 5(4,0%), Enterobacter aerogenes 4 (3,2%), Acinetobacter spp 2(1,6%), Pseudomonas aeruginosa 4(3,2%), Klebsiella Pneumoniae 1(0,8%) y Escherichia coli 1(0,8%). Conclusiones: El aislamiento de bacterias patógenas sugiere que el estetoscopio debe ser considerado como un vector de la infección nosocomial.

Transferable cefoxitin resistance in enterobacteria from Greek hospitals and characterization of a plasmid-mediated group 1 beta-lactamase (LAT-2).

1996 ◽  
Vol 40 (7) ◽  
pp. 1736-1740 ◽  
Author(s):  
M Gazouli ◽  
L S Tzouvelekis ◽  
E Prinarakis ◽  
V Miriagou ◽  
E Tzelepi
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Klebsiella Pneumoniae ◽  
Enterobacter Aerogenes ◽  
Citrobacter Freundii ◽  
Beta Lactamase ◽  
Beta Lactamases ◽  
Greek Hospitals ◽  
Group 1

Cefoxitin resistance in Klebsiella pneumoniae from Escherichia coli strains isolated in Greek hospitals was found to be due to the acquisition of similar plasmids coding for group 1 beta-lactamases. The plasmids were not self-transferable but were mobilized by conjugative plasmids. These elements have also been spread to Enterobacter aerogenes. The most common enzyme was a Citrobacter freundii-derived cephalosporinase (LAT-2) which differed from LAT-1 by three amino acids.

scholarly journals Chemical and biological evaluation of rejects from the wood industry

2005 ◽  
Vol 48 (spe) ◽  
pp. 237-241 ◽  
Author(s):  
Daniel Granato ◽  
Domingos Sávio Nunes ◽  
Patrícia Póvoa de Mattos ◽  
Ester de Moura Rios ◽  
Adeline Glinski ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Ethyl Acetate Extract ◽  
Micrococcus Luteus ◽  
Chemical Characterization ◽  
Enterobacter Aerogenes ◽  
Ethanol Extract ◽  
Biological Evaluation ◽  
Wood Industry ◽  
Anatomical Studies ◽  
E Coli

This study aimed chemical characterization and microbiological evaluation of extracts obtained from the waste of woods marketed in Paraná State: Peroba-Rosa (Aspidosperma sp.), Roxinho (Peltogyne sp.), Jatobá(Hymenaea sp.), Curupixá (Micropholis sp.), Itaúba (Mezilaurus sp.), Cedrilho (Erisma sp.) and Imbúia (Licaria sp.), whose botanical identifications were based on anatomical studies. The extracts were prepared with different solvents, analyzed by TLC and UV/VIS techniques, and tested against: Proteus mirabilis ATCC15290, Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, Enterobacter aerogenes ATCC13048, Micrococcus luteus ATCC9341, Klebsiella pneumoniae ATCC13883, Pseudomonas aeroginosa ATCC27853, Staphylococcus aureus, Streptococcus mutans and Bacillus cereus isolated from the clinic. The ethanol extract from Peroba-rosa containing alkaloids showed activity against P. mirabilis. Itaúba, Jatobá and Imbúia methanol extracts containing phenolics, and the Roxinho ethyl acetate extract containing terpenoids and phenolics were active against K. pneumoniae, M. luteus, E. coli, S. aureus and P. mirabilis. P. aeroginosa, S. mutans and E. aerogenes were resistant to the extracts.

scholarly journals MIKROORGANISME PADA SALIVA ANAK NORMAL DAN ANAK AUTISME

2019 ◽  
Vol 5 (1) ◽  
pp. 38-44
Author(s):  
Nadhifah Salsabila ◽  
Valendriyani Ningrum ◽  
Leny Sang Surya
Keyword(s):  
Staphylococcus Aureus ◽  
Cross Sectional ◽  
Candida Sp

Pendahuluan: Autisme merupakan suatu gangguan perkembangan yang mempengaruhi fungsi normal otak, perkembangan interaksi sosial dan kemampuan berkomunikasi. Anak autisme memiliki kepedulian yang rendah terhadao diri sendiri, sensitif terhadap bulu sikat dan pasta gigi, cenderung mengkonsumsi makanan kariogenik, dan memiliki kelainan motorik lidah. Keterbatasan yang dimiliki anak autisme tersebut menyebabkan anak autisme lebih berisiko terhadap penyakit periodontal dan karies gigi. Karies merupakan penyakit jaringan keras gigi akibat aktivitas mikroorganisme dalam suatu karbohidrat yang dapat diragikan dan penyakit periodontal adalah penyakit pada jaringan pendukung gigi yang faktor penyebab utamanya adalah mikroorganisme, kedua penyakit tersebut erat kaitan terjadinya dengan kondisi rongga mulut salah satunya adalah saliva. Tujuan penelitian ini adalah untuk mengetahui perbedaan jenis dan jumlah koloni mikroorganisme pada saliva anak autisme dan anak normal yang mengalami karies. Metode: Penelitian ini menggunakan pendekatan cross sectional, subjek penelitian anak autisme yang berada di SLB Autisma YPPA Kota Padang dan anak normal yang berada di SD N 05 Sawahan Kota Padang. Sampel penelitian berupa saliva dilakukan pembiakkan mikroorganisme pada media agar darah. Data dianalisis menggunakan uji Mann-Whitney. Hasil: Terdapat perbedaan jenis mikroorganisme pada saliva anak normal dan anak autisme. jenisMikroorganisme pada saliva anak autisme yaitu Streptococcus sp, Neisseria sp, Staphylococcus aureus, dan Candida sp, sementara pada saliva anak normal jenis Streptococcus sp dan Neisseria sp. Hasil uji statistik perbedaan jumlah Streptococcus sp didapatkan nilai p=0,001, pada Neisseria sp didapatkan nilai p=0,012. Kesimpulan: Terdapat perbedaan jenis dan jumlah koloni mikroorganisme pada saliva anak normal dan anak autisme.

scholarly journals Microbial Assessment of Bed Linens in Ekiti State University Students’ Hostels

2020 ◽  
pp. 43-52
Author(s):  
T. O. Olowomofe ◽  
J. O. Oluyege ◽  
A. O. Ogunlade ◽  
D. T. Makinde
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Proteus Mirabilis ◽  
Disease Transmission ◽  
Enterobacter Aerogenes ◽  
Antibiotics Resistance ◽  
Klebsiella Aerogenes ◽  
State University ◽  
Plasmid Profiling

Bed linen is clearly recognized as a potential reservoir for microorganisms and could be a vector of disease transmission. The present study was aimed at isolating and characterizing bacteria and fungi from different kinds of bed linen of student in Ekiti State University hostels. Pour plate method was used for the enumeration of total bacteria count from the posterior and anterior end of the bed linen. The average bacteria count for the anterior and posterior end was 7.46 and 7.16 Log10 CFU/ml respectively. The most dominant microbial species were bacteria and these were mostly found in the environment and on human skin. The bacteria isolated were Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis, Enterobacter aerogenes, Bacillus cereus, Escherichia coli and Klebsiella aerogenes. Bacillus cereus had the highest frequency of occurrence (25%), followed by Staphylococcus aureus (15%), Escherichia coli (15%), Klebsiella aerogenes (15%), Staphylococcus epidermis (15%), Enterobacter aerogenes (10%) and Proteus mirabilis (5%). The fungi isolates were Aspergillus sulphureus, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus and Fusarium spp. Antibiotics susceptibility test was carried out on the bacteria isolates with gram negative bacteria showing resistance to Cotrimoxazole and gram positive bacteria showing resistance to Amplicillin. Most of the bacteria isolates have multiple antibiotics resistance. The bacterial isolates were susceptible to Ciprofloxacin. Plasmid profiling was also done with Escherichia coli having three high molecular weight plasmids, Bacillus cereus and Enterobacter aerogenes do not possess plasmid. The identified species are suspected to be opportunistic pathogens for human, representing a risk for people with weakened immune system, especially in cases of super-infection.

scholarly journals Profil Penggunaan Antibiotik dan Peta Kuman pada Pasien Gangren Diabetes Melitus di Sebuah RSUD di Kabupaten Gresik

2020 ◽  
Vol 3 (2) ◽  
pp. 96
Author(s):  
Isna Romadhona ◽  
Fauna Herawati ◽  
Rika Yulia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Acinetobacter Baumannii ◽  
Proteus Mirabilis ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Diabetes Melitus

Antibiotik merupakan obat yang digunakan untuk mengatasi dan mencegah infeksi bakteri. Penggunaan antibiotik yang tidak tepat dapat menimbulkan berbagai masalah, diantaranya pengobatan akan lebih mahal dan juga risiko terjadinya resistensi bakteri terhadap antibiotik. Penelitian ini bertujuan untuk mengetahui profil penggunaan antibiotik dan profil peta kuman pada pasien gangren diabetes melitus di sebuah RSUD di Kabupaten Gresik serta untuk mengetahui kesesuaian penggunaan antibiotik dengan mengacu pada Permenkes Republik Indonesia No. 2406/Menkes/PER/XII/2011. Data penggunaan antibiotik diperoleh dari catatan Rekam Medis pada periode Januari – November 2017. Data penggunaan antibiotik dihitung dengan menggunakan rumus DDD/100 pasien-hari rawat. Hasil perhitungan DDD/100 pasien-hari rawat menunjukkan hasil sebesar 470,11 DDD/100 pasien-hari rawat. Peta kuman pada pasien gangren, melaporkan adanya bakteri Enterobacter cloacae 24%, Escherichia coli 18%, Staphylococcus aureus 15%, Acinetobacter baumannii 9%, Pseudomonas aeruginosa 6%, Citrobacter youngae 6%, Enterobacter aerogenes 6%, Proteus vulgaris 6%, Staphylococcus schleiferi 6%, Klebsiella pneumoniae 3%, dan Proteus mirabilis 3% . Penggunaan antibiotik seftriakson dan metronidazol pada pasien gangren diabetes melitus di sebuah RSUD di Kabupaten Gresik pada periode Januari – November 2017 telah sesuai dengan pedoman penggunaan antibiotik berdasarkan Permenkes Republik Indonesia No. 2406/Menkes/PER/ XII/2011, yaitu antibiotik golongan sefalosporin generasi III yang lebih aktif terhadap Enterobacteriaceae dan antibiotik golongan nitroimidazol yang dapat mengobati infeksi bakteri basil anerob Gram-Negatif.

scholarly journals Thin-layer chromatographic technique for rapid detection of bacterial phospholipases

1975 ◽  
Vol 2 (5) ◽  
pp. 373-376
Author(s):  
N J Legakis ◽  
J Papavassiliou
Keyword(s):  
Escherichia Coli ◽  
Thin Layer ◽  
Rapid Detection ◽  
Enterobacter Aerogenes ◽  
Egg Yolk ◽  
Chromatographic Technique ◽  
Citrobacter Freundii ◽  
Soluble Product ◽  
Resting Cell ◽  
Layer Chromatography

Silica gel thin-layer chromatography was employed to detect lecithinase activity induced from bacterial resting cell preparations induced from bacterial resting cell preparations incubated at 37 C for 4 h in the presence of purified egg yolk lecithin. Bacillus subtilis, Bacillus cereus, Serratia marcescens, and Pseudomonas aeruginosa hydrolyzed lecithin with the formation of free fatty acids as the sole lipid-soluble product. In none of the Escherichia coli and Citrobacter freundii strains tested could lecithinase activity be detected. Four among eight strains of Enterobacter aerogenes and one among 12 strains of Proteus tested produced negligible amounts of free fatty acid.

scholarly journals Research of properties of traditional Carpathian cheeses

2019 ◽  
Vol 21 (91) ◽  
pp. 69-78 ◽  
Author(s):  
O. Y. Tsisaryk ◽  
I. M. Slyvka ◽  
L. Y. Musiy
Keyword(s):  
Staphylococcus Aureus ◽  
Salt Content ◽  
Experimental Studies ◽  
Enterobacter Aerogenes ◽  
Sour Milk ◽  
Physico Chemical ◽  
Staphylococcus Epidermis ◽  
Microbiological Parameters ◽  
Color Figure ◽  
Organoleptic Parameters

The purpose of the work was to investigate the organoleptic, physico-chemical and microbiological indices of traditional Carpathian cheeses, in particular, brynza, butz and vurda, made directly on the mountain valleys with traditional technology in Rakhiv district of the Transcarpathian region. Of 16 samples of cheese, 13 samples were cheese butz (samples 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15); vurda cheese (samples 4 and 16) and cheese of brynza a year ago (sample 14). Experimental studies of organoleptic, physico-chemical and microbiological parameters of cheese samples were carried out in the laboratory of the milk and milk products department and in the department of biotechnology at Rzeszow University (Poland). In samples of cheese, organoleptic parameters were studied in accordance with GOST 7616-85; active acidity – by potentiometric method using the pH meter of the ARN-9 brand; titrated acidity – titrarily (in degrees Turner) according to GOST 3624-67. The mass fraction of salt in the cheese was determined by titration of the extract with nitric oxide silver (GOST 3627-81). The detection of bacteria of the genus Staphylococcus aureus was determined by sowing on a solid Gissa with mannitol, with further detection and confirmation of the belonging of the grown colonies to Staphylococcus aureus (GOST 30347-97). The detection of bacteria of the genus Salmonella was determined by sowing on the Endo solid medium (GOST 31659-2012). The bacteria of the intestinal stem group were determined by sowing on a Kessler liquid medium (GOST 9225-84). Cultivation of microorganisms was carried out at temperature +37 °С for 48 h. According to researches of organoleptic parameters of cheeses it was found that samples of cheese butz were characterized by sour milk, and samples of 3 and 5 sour taste and aroma, a dense humus consistency and from gray to white color. Figure – with single small and large bodies of irregular shape. According to studies of organoleptic parameters of cheese, the taste and aroma of the product should be given – light sour milk, salted, which is confirmed by the salt content – 4.6%. Consistency was crumbly, the color was yellow. Samples of cheese vurda were characterized by delicate butter, sour milk taste and smell, paste, crumbly consistency and white color. Analyzing the acidity of butz cheese it was that it was high particularly in samples 3, 8 and 14, which was reflected in organoleptic parameters. In the samples of vurda titrated acidity was 75 °T in sample 4 (pH 5.7) and 16 °T in sample 16 (pH 6.4), while brynza was characterized by the highest acidity – 260 °T (pH 4.9). According to microbiological parameters, all specimens were characterized by the presence of pathogenic microflora. In particular, 1, 3, 5–9, 11–15 samples showed Staphylococcus aureus, 10 sample of Staphylococcus epidermis, 2 sample of bacteria of the genus Salmonella, 16 sample of Enterobacter aerogenes, and 4 samples of mold.

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