scholarly journals Assessment of the efficacy of using taurine supplements to improve growth and feed utilization of juvenile starry flounder (Platichthys stellatus) given diets based on soy-protein

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10597
Author(s):  
Peiyu Li ◽  
Hongyi Bu ◽  
Baoshan Li ◽  
Yongzhi Sun ◽  
Meiqi Wang ◽  
...  
Keyword(s):  
Oxidation Resistance ◽  
Glycogen Synthase ◽  
Juvenile Fish ◽  
Fatty Acid Synthetase ◽  
Control Treatment ◽  
Circulation System ◽  
Average Weight ◽  
Crude Lipid ◽  
Starry Flounder ◽  
Platichthys Stellatus

A feeding trial was conducted to assess the feasibility of supplementing taurine in soy-based diets for juvenile starry flounder Platichthys stellatus. The basal diet (Crude protein 66.5%, crude lipid 8.5%) was supplemented with 0 (control), 0.5%, 1.0%, 1.5%, 2.0% and 2.5% taurine to formulate six test diets. Each diet was fed to 40 juvenile fish (22.25 g) in triplicate tanks (120 L) attached to a sea water circulation-system. Fish were fed twice daily by hand to apparent satiation during the 56-d trial. At the end of the trial, fish were counted and weighed for the analyses of growth performance, diet utilization and survival after a 24-h fast. Blood, intestines and muscles were collected for the analyses of serum oxidation resistance, digestive enzymes and body compostion. Livers were collected from the remaining fish at 4 h post-feeding for metabolic enzymes analyses. The results showed that fish fed diets supplemented with 1.0–2.5% taurine grew from 22.25–22.26 g to 47.88–50.40 g with higher average weight gain (25.62–28.12 vs 23.07 g ), specific growth rate (1.37–1.46 vs 1.27%/d ), feed intake (1.04–1.06 vs 1.00%/d), protein efficiency (2.50–2.61 vs 2.44) and lower feed conversion rate (0.84–0.83 vs 0.89) than the control treatment. Diets supplemented with 1.5–2.5% taurine significantly elevated the activities of pepsin (2.47–2.55 vs 2.22, U mg−1 prot), trypsin of distal intestine(14.55–15.24 vs 11.94, U mg−1 prot), hepatic glucokinase (126.62–129.42 vs 105.56, U mg−1 prot) and fatty acid synthetase (125.56-136.89 vs 108.45, U mg−1 prot). All diets supplemented with taurine increased the activities of lipase (32.23–36.67 vs 29.53, U g−1 prot) and trypsin (35.85–37.89 vs 33.54, U mg−1 prot) of proximal intestine, hepatic aspartate transaminase (736.990–832.38 vs 699.24, U mg−1 prot), alanine aminotransferase (477.40–551.86 vs 373.97, U mg−1 prot) and glycogen synthase (2.16–2.59 vs 1.97, U mg−1 prot), as well as serum superoxide dismutase (4.33–4.59 vs 4.07, U mg−1 prot ) and glutathione peroxidase (42.23–50.25 vs 39.17, mol mg−1 prot). Therefore, taurine supplementation benefits juvenile starry flounder growth, digestion, nutrients metabolism and oxidation resistance. The optimal taurine requirement for starry flounder is 1.75%, and the recommended supplementation level is at least 1.6% for maximizing growth of fish fed a low-fishmeal diet (13.6%).

scholarly journals Nutrient metabolism in the liver and muscle of juvenile blunt snout bream (Megalobrama amblycephala) in response to dietary methionine levels

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ke Ji ◽  
Hualiang Liang ◽  
Mingchun Ren ◽  
Xianping Ge ◽  
Liangkun Pan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Binding Protein ◽  
Juvenile Fish ◽  
Fatty Acid Synthetase ◽  
Initiation Factor ◽  
Megalobrama Amblycephala ◽  
Mrna Levels ◽  
Nutrient Metabolism

AbstractA 75-day rearing trial was designed to study the response of juvenile Megalobrama amblycephala to dietary methionine (Met) levels. Three practical diets with graded Met levels (0.40%, 0.84% and 1.28% dry matter) were prepared to feed the juvenile fish. The results showed that the 0.84% Met diet significantly improved the growth compared with 0.40% diets. Compared with 0.84% and 1.28% Met, 0.40% Met significantly increased the hepatic lipid content, while decreasing the muscular lipid and glycogen contents. 0.40% Met decreased the protein levels of phospho-Eukaryotic initiation factor 4E binding protein-1 (p-4e-bp1), 4e-bp1 and Ribosomal protein S6 kinase 1 in the liver, compared with 0.84% diet, while an increasing trend was observed in the muscle. Met supplementation tended to decrease and increase lipid synthesis in the liver and muscle, respectively, via changing mRNA levels of sterol regulatory element-binding protein 1, fatty acid synthetase and acetyl-CoA carboxylase. 1.28% dietary Met promoted fatty acid β-oxidation and lipolysis in both the liver and muscle by increasing carnitine palmitoyl transferase 1, peroxisome proliferator activated receptor alpha, lipoprotein lipase and lipase mRNA levels. Compared with 0.40% and 0.84% dietary Met, 1.28% Met enhanced the mRNA levels of hepatic gluconeogenesis related genes phosphoenolpyruvate carboxykinase (pepck), and glucose-6-phosphatase, and muscular glycolysis related genes phosphofructokinase (pfk), and pyruvate kinase (pk). The mRNA levels of hepatic pfk, pk and glucokinase were markedly downregulated by 1.28% Met compared with 0.84% level. Muscular pepck, glycogen synthase, and hepatic glucose transporters 2 mRNA levels were induced by 1.28% Met. Generally, deficient Met level decreased the growth of juvenile Megalobrama amblycephala, and the different nutrient metabolism responses to dietary Met were revealed in the liver and muscle.

scholarly journals Oral application of probiotic, prebiotic, and synbiotic in Pacific white shrimp(Litopenaeus vannamei)challenged with Vibrio harveyi

2013 ◽  
Vol 11 (1) ◽  
pp. 54 ◽  
Author(s):  
. Widanarni ◽  
Puguh Widagdo ◽  
Dinamella Wahjuningrum
Keyword(s):  
Survival Rate ◽  
Vibrio Harveyi ◽  
Positive Control ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Control Treatment ◽  
Resistant Bacteria ◽  
Average Weight ◽  
High Survival ◽  
Immersion Method

<p>The use of antibiotics for controlling of luminous vibriosis caused by <em>Vibrio harveyi</em> is restricted now, because it induces antibiotic-resistant bacteria and leave residue in shrimp’s body. An alternative solution that can be done to treat the disease is by using applications of probiotic, prebiotic, and synbiotic. The aim of this research was to examine the effect of probiotic, prebiotic, and synbiotic on the survival rate and growth of Pacific white shrimp against <em>V. harveyi</em> infection. Feed as a treatment was supplemented with probiotic 1%, prebiotic 2%, and probiotic 1%+prebiotic 2% (synbiotic). Shrimps feed without supplementation of probiotic and prebiotic was used as a control treatment. The shrimps were maintainedin the aquarium (60×30×35 cm<sup>3</sup>) with a density of 40 shrimps/40 L and an average weight of 0.4±0.1 g. After 30 days of feeding treatment, the shrimp was challenged by immersion method with <em>V. harveyi</em> solution containing 10<sup>6</sup> CFU/mL. The results showed that before challenge, synbiotic feed treated shrimp has a growth rate (5.89%), feed conversionrate (1.21), and a high survival rate (80%). After challenge, survival rate (83.33%) of shrimp fed diet supplemented with synbiotic was higher than prebiotic (51.67%) and positive control (31.67%).</p> <p>Keywords: probiotic, prebiotic, synbiotic, <em>Vibrio harveyi</em>,Pacific white shrimp</p>

scholarly journals The affection of fine bubbles (FBs) aplication on growth, feed efficiency of striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878) and water quality in aquaponic system

2021 ◽  
Vol 21 (3) ◽  
pp. 277-290
Author(s):  
Yuli Andriani ◽  
Zahidah Zahidah ◽  
Rosidah Rosidah ◽  
Iskandar Iskandar
Keyword(s):  
Water Quality ◽  
Feed Efficiency ◽  
Suitable Condition ◽  
Plant Productivity ◽  
Quality Parameters ◽  
Control Treatment ◽  
Average Weight ◽  
Feed Conversion ◽  
Pangasianodon Hypophthalmus ◽  
Randomized Design

This research aims to determine the effect of fine bubble technology with different pressures on Siamese catfish seed's growth and feed efficiency in an aquaponic system. This research used a completely randomized design with four treatments and three replications. Treatment A (Control), Treatment B (FBs with a pressure of 4.5 atm), Treatment C (FBs with a pressure of 5 atm and treatment D (FBs with a pressure of 5.5 atm). The parameters observed were Specific Growth Rate, Feed Conversion Ratio, water quality (temperature, pH, DO, and ammonia), and plant productivity.  The seeds used were catfish seeds aged 16-36 days, 1-2 inches long, and weighed 0.26-0.29 grams. The container used is a fiber tub of 16 units with a size of 70 cm x 70cm x 70 cm. The feed used is commercial feed PF 1000 content crude protein 35%. The data were analyzed using variance with the F test at the 95% confidence level. The best treatment for catfish growth was treatment D (pressure 5.5 atm), which gave the highest SGR value of 7.24 ± 0.5 and FCR value of 1.19 ± 0.09. The value of water quality parameters is in suitable condition for the growth of catfish seed. The best plant productivity was found in treatment D with an average weight of 60.75 g.

scholarly journals Response of vegetative growth and chemical properties in tomato plant (Tariz) for organic fertilization.

2019 ◽  
pp. 51-66
Author(s):  
Halemah M. Amhamed ◽  
Ahmed. I. Sharaf ◽  
Salaheddin B. Elbelazi ◽  
Moftah A. Assofal ◽  
Amal E. Alhishni
Keyword(s):  
Tomato Plant ◽  
Agricultural Land ◽  
Block Design ◽  
Organic Fertilizer ◽  
Chemical Properties ◽  
Cattle Manure ◽  
Control Treatment ◽  
Average Weight ◽  
Organic Fertilization ◽  
And Chemical Properties

The experiment was carried out in agricultural land in Sorman between 4 - 2017 to 7 - 2017, in the design of Randomized Complete Block Design (RCBD) to study the response of vegetative traits and chemical properties in the tomato plant of Tariz for organic fertilization, by 6 treatments and 3 replicates, For SPSS statistical analysis at a significant level of LSD 0.05). The experiment included four types of semi-decomposed organic fertilizer (sheep manure, cattle manure, camel manure, horse manure) in 6 treatments, in addition to the control treatment, which included four treatments for each type of organic fertilizer as: fertilizer: soil by ratio (1: 3) that is 5Kg of fertilizer : 15 kg of soil, mixed treatment of fertilizer : soil (1: 1) (10 kg of fertilizer: 10 kg of soil), and double treatment (3: 1) (15 kg of fertilizer: 5 kg soil). The seedlings were planted in experimental units of 7.20 m2. The seedlings were treated in biological control using the garlic extract for insect protection after 15 days of planting, as well as the addition of simple proportions of hay as a cover to reduce the growth of herbs. The experiment showed that the treatment of cattle manure significantly increased most of the studied morphological traits (length of root, plant length, number of flowers, number of leafy branches), as well as significant increase in fresh and dry weight of leaves and average weight of five fruits.. There were significant differences in the content of the N, P in leaves and NPK elements in the fruits, while there was a significant increase in vitamin C content in fruits for all treatments.

scholarly journals EFFECT OF GRADUAL HIGH SALINITY ON SOME STRESS PARAMETERS (GLUCOSE,TOTAL PROTEIN AND LACTATE) IN BLOOD PLASMA OF COMMON CARP

2017 ◽  
Vol 48 (2) ◽  
Author(s):  
Al- Khshali & Al- Hilalli
Keyword(s):  
Blood Plasma ◽  
Common Carp ◽  
Total Protein ◽  
High Salinity ◽  
Tap Water ◽  
Control Treatment ◽  
Average Weight ◽  
Water Control ◽  
Stress Parameters ◽  
In Blood Plasma

The present study was conducted to investigate the effect of high salinity on the some stress parameters of common carp (Cyprinus carpio) which gradually exposed to salt concentrations of 5, 10 and 15g/liter, as well as tap water (control 0.1g/liter) for 90 days .80 fish were randomly distributed on eight glass tanks with 2 replicates as 10 fish / replicate at average weight of 15 ± 3 g to study the effect of salinity on the Total protein , Glucose ,and Lactate in blood plasma , Fish were fed during the trial on commercial diet with 31.9 % protein content.. Results showed that an increase in the total protein to 4.98 and then decrease to 4.60  and 3.90 g/100 cm3 when the salinity increased to 5, 10 and 15 g/l respectively, compared with the control treatment (4.94 g/100 cm3).  Glucose reached 75.11, 91.20 and 95.17 mg/100 ml at the concentrations of 5, 10 and 15 g/l respectively, compared with the control treatment (66.64 mg/100ml). Lactate also increased to 43.12, 45.38  and 48.53  mg/100 ml , when the salinity increased to  5 , 10 and 15 g/l respectively, in comparison with control treatment  (40.65 mg/100 ml) .

Sign in / Sign up

Export Citation Format

Share Document