Reproductive Toxicity & Biomarker Response of Male Albino Rats (Rattus norvegicus) to a Daily Dose of Local Gin (Ogogoro)

2019 ◽  
pp. 1-7
Author(s):  
E. Oriakpono, Obemeata ◽  
N. Gbule, Ronald
Keyword(s):  
Blood Cell ◽  
Sperm Count ◽  
Reproductive Toxicity ◽  
Blood Parameters ◽  
Albino Rats ◽  
Sodium Potassium ◽  
Biomarker Response ◽  
Significant Difference ◽  
Average Control ◽  
The Mean

This study evaluates the effect of local gin (ogogoro) on Hepato-renal parameters such as aspartate amino transferase, alanine amino transferase, sodium, potassium, chlorine and bicarbonate, haematological parameters such as total protein, packed cell volume, red blood cell, white blood cell haemoglobin, platelet and lymphocytes and sperm count parameter. The results show that: The mean serum electrolytes were for week 1 (Na 165.0, K 5.27, Cl 99.67 and HCO3 19.67), week 2 (Na 138, K 5.77, Cl 89.67 and HCO3 20), week 3 (Na 126.67, K 3.67, Cl 87.67 and HCO3 19) and week 4 (Na 117.67, K 2.70, Cl 73.67 and HCO3 22) and showed a significant difference in Na, Cl and HCO3 only when compared with the average control at (P<0.05), AST had a mean of 30.0 in week 1 which increased to 45.0 in week 4 while ALT had a mean of 15.0 in week 1 and increased to 30.67 in week 4. The mean serum protein reduced from 51.15 in week 1 to 42.53 in week 4 with significant difference (P<0.05). Mean PCV reduced from 36.0 in week 1 to 24.40 in week 4, Hb from 12.07 in week 1 to 8.80 in week 4 with a significant difference (P<0.05) when comparing the test with the average control, WBC from 6.17 in week 1 to 5.40 in week 4, Platelet increased from a mean of 255 on week 1 to 683 on week 4 with significant difference (P<0.05), RBC had a mean of 5.27 in week 1 and 5.25 on week 4 with no significant difference (P>0.05). Lymphocyte reduced from a mean of 69.0 week 1 to 45.50 in week 4 but when the test was compared with the average control it had a significant difference (P<0.05). While the mean sperm count was 275 in week 1 and 325 in week 4. These investigations demonstrated that local gin changes blood parameters which could lead to anaemia in mammals when constantly taken and also cause a detrimental effect on sperm count which could cause infertility in males as well as kidney and liver disease.

scholarly journals Reproductive & Biomarker Response of Male Albino Rats (Rattus norvegicus) to a Daily Dose of Soft Drink

2019 ◽  
pp. 1-8
Author(s):  
Oriakpono, Obemeata E. ◽  
Owolabi, Iyabo S.
Keyword(s):  
Blood Cell ◽  
Sperm Count ◽  
Soft Drink ◽  
Test Group ◽  
Mean Value ◽  
Male Rats ◽  
Biomarker Response ◽  
Significant Difference ◽  
Average Control ◽  
The Mean

The effect of a daily consumption of soft drink was evaluated using 24 albino male rats divided into two groups viz:control and treatment. The experiment was carried out for four (4) weeks. The treatment (a brand of soft drink) was administered to the test group for three weeks while on the fourth week no treatment was given to the test group. The parameters analysed include; Sperm count, kidney function test, liver test, red blood cell, pack cell volume, haemoglobin, white blood cell, platelets, lymphocytes. The results showed that: The mean serum electrolyte for Na (mmol/l) was low for week 1, 2, 3 and 4 having 142, 140, 133.6 and 141.66 respectively when compared to the average control (147.3) with a significant difference (P<0.05) in week 1 and 4, K (mmol/l)  were all lower than the average control (5.4) across the week with no significant difference (P>0.05) but had the least mean value of 4.8 in week 2. Bicarbonate (mmol/l) was also significantly lower (P<0.05) in the treated group when compared to the average control (24.3) with the least mean value in week 4 (18.67) and Cl (mmol/l) had a mean of 93.0 in week 1, 94.67 in week 2, 108.66 in week 3 and 107.67 in week 4 with an average control of 99.33. AST (U/L) mean value was 20.67 in week 1 which increased to 31.67 in week 4 while ALT (U/L) mean value was 10 in week 1 which also increased to 13 in week 4. The mean serum protein (g/dL) reduced from 81.83 in week 1 to 73.24 in week 4. Mean PCV (%) reduced from 33.67 in week 1 to 32.7 in week 4, Hb (g/dL) increased from 11.2 in week 1 to 13.4 in week 4 with a significant difference (P<0.05) when comparing the test with the average control, WBC (X109) increased from a mean  5.26 in week 1 to 11.9 in week 4 with a significant difference (P<0.05), Platelet (X109) mean value was 315 on week 1 and 419 in week 4 with significant difference (P<0.05) in week 3 and 4 when compared with its control, RBC (X1012) increased from a mean of 4.23 in week 1 to 6.90 in week 4 with significant difference (P<0.05). Lymphocyte (X109) mean value for week 1 was 70 and 82.26 in week 4 with significant difference (P<0.05) across the week. While the mean sperm count (X106) reduced significantly (P<0.05) from 425 in week 1 to 400 in week 4 when compared to the average control (566). These findings demonstrate that regular consumption of soft drink had a detrimental effect on the sperm count, liver, kidney and on the haematological parameters.

scholarly journals Reproductive & Biomarker Response to a Daily Dose of Instant Noodle Seasoning in Male Albino Rats (Rattus norvegicus)

2019 ◽  
pp. 1-8
Author(s):  
E. Oriakpono, Obemeata ◽  
C. Ibanibo, Blessing
Keyword(s):  
Sperm Count ◽  
Monosodium Glutamate ◽  
Treated Group ◽  
Albino Rats ◽  
Daily Consumption ◽  
Biomarker Response ◽  
Significant Difference ◽  
Average Control ◽  
The Mean ◽  
Instant Noodle

The effect of a daily consumption of Instant noodle seasoning containing the Monosodium glutamate (MSG) on rat was evaluated, The parameters investigated include; Alkaline aminotransferase (ALT), Aspartate aminotransferase  (AST). Hemoglobin (Hb), packed cell volume (PCV) white blood cell (WBC), protein, platelets, lymphocytes and Serum electrolytes; sodium (Na+), potassium (K+) chloride (Cl), bicarbonate (HCO3 - ). Sperm count was also investigated. The results revealed the following, the mean PCV was 29 and 25.13on week 1 and week 4, with an average control of 30.69, mean Hb was 10 in week 1 and 6.57 in week 4, RBC had an average control of 5.28 while week 1 had a mean of 4.77 and week 4 3.67, there was a significant difference (P<0.05) for PCV and Hb. The mean WBC and Lymphocyte were 6 and 61 in the first week, and 5.8 and 60.17 on the fourth week, with an average control of 5.28 for WBC and 77.53 for lymphocytes. Platelet had a mean of 251 on the first week and a mean of 532 on the fourth week with a significant difference across the group in WBC and platelets (P<0.05). The           mean serum Na, K and Cl reduced from 140.67, 4.13 and 100.67 in week 1 to 116, 2.5 and 98 in week 4 with a significant difference (P<0.05) across the group when compared to the average control for Na and K. HCO3 had a mean of 23.67 in week 1 and a mean of 22.67 in week 4 in the treated group. AST had a mean of 24 in week 1 which increased to 41.67 in week 4 while ALT increased from a mean of 4.00 in week 1 and 28 in week 4 with a significant difference (P<0.05) across the group. The mean serum protein was 51.93 in week 1 and a 74.29 in week 4. The           mean sperm count was 800, 299.67, 450.67 and 501 for week 1, 2, 3 and 4 respectively. The results indicates that daily consumption of Instant noodle seasoning may cause liver damage,            and kidney dysfunction and has been discovered to have negative effects on blood and sperm cells.

scholarly journals Effects of Telfairia occidentalis (Hook F.) Methanol Leaf Extract on the Haematological Indices and Histomorphology of the Endometrium and Liver of Female Albino Rats

2016 ◽  
Vol 8 (3) ◽  
pp. 272-276
Author(s):  
Chidozie Nwabuisi OKOYE ◽  
David OGWU ◽  
John I. IHEDIOHA ◽  
Izuchukwu S. OCHIOGU ◽  
Anietie F. UDOUMOH ◽  
...  
Keyword(s):  
Blood Cell ◽  
Histological Examination ◽  
Leaf Extract ◽  
Hematological Parameters ◽  
Female Rats ◽  
Albino Rats ◽  
Telfairia Occidentalis ◽  
Significant Difference ◽  
The Mean ◽  
Liver Tissues

The objective of this study was to evaluate the effects of administration of Telfairia occidentalis (Hook f.) methanol leaf extract on some hematological indices and histomorphology of the uterus and liver of female albino rats. Acute toxicity study of the extract was performed with female rats, following standard procedure. They were randomly assigned into 3 groups. The first group (A) served as the untreated control and received distilled water, while the second group (B) and third (C) received 200 and 800 mg/kg mg/kg body weight of the methanol leaf extract of T. occidentalis respectively. Administration of the extract was done daily via the oral route for 21 days. Blood was collected for hematological evaluation at weekly intervals. Hematological parameters assessed were the packed cell volume (PCV), red blood cell (RBC) count, hemoglobin concentration (HB) and total white blood cell (TWBC) count. At the end of the study period uterine and liver tissues were excised and prepared for histological examination. Results showed that there was a significant decrease (P < 0.05) in the mean PCV, RBC and HB values of the treatment groups, but only at the end of the second week of administration of the extract. There was no significant difference (P > 0.05) in the mean total WBC throughout the duration of the study. Histological examination of uterine and liver tissues of the different groups did not show any lesions. It was concluded that the T. occidentalis leaf methanolic extract does not adversely affect the reproductive functions of the uterus.

scholarly journals Effect of Tramadol on Sperm Profile of Male Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
I. S. Esua ◽  
U. U. Uno ◽  
U. B. Ekaluo
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Head ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Sperm Viability ◽  
Albino Rat ◽  
Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

The Treatment of Salicylate Poisoning Using Mannitol and Forced Alkaline Diuresis

1970 ◽  
Vol 38 (3) ◽  
pp. 327-337 ◽  
Author(s):  
K. Prowse ◽  
M. Pain ◽  
A. D. Marston ◽  
G. Cumming
Keyword(s):  
Serum Potassium ◽  
Serum Levels ◽  
Urine Flow ◽  
Serum Electrolyte ◽  
Acid Base ◽  
Sodium Potassium ◽  
Salicylate Poisoning ◽  
Significant Difference ◽  
Rapid Removal ◽  
The Mean

1. A comparison has been made between alkaline mannitol diuresis and alkaline glucose diuresis in the treatment of salicylate poisoning during an 8 hr period. 2. Mannitol gave a higher rate of urine flow, but there was no significant difference in the rate of salicylate excretion. 3. Serum levels of sodium, potassium and calcium fell in both groups, mainly during the first 2 hr. The mean fall in serum sodium was greater (21·4 mEq/l compared with 10·0 mEq/l) and that in serum potassium less (0·6 mEq/l compared with 1·5 mEq/l) in patients who received mannitol than in those who did not. 4. All serum electrolyte values had returned to normal within 24 hr of the end of the treatment period. 5. Acid—base changes occurring during treatment are described. 6. No adverse clinical features were seen in either treatment group. 7. Syrup of ipecacuanha was used to induce emesis. The mean recovery of salicylate in the vomitus was 38% of the apparent total ingested. The variability in recovery (0–81%) was not related to the delay between ingestion and vomiting. 8. It is concluded that mannitol does not increase the excretion of salicylate. It maintains good urinary excretion, thereby increasing the safety of the procedure. It is associated with a smaller fall in serum potassium and permits rapid removal of salicylate for a smaller rise in serum pH than does forced alkaline diuresis alone.

scholarly journals Effect of heat stress on blood parameters in indigenous sheep

2014 ◽  
Vol 12 (1) ◽  
pp. 91-94 ◽  
Author(s):  
MS Rana ◽  
MA Hashem ◽  
MN Sakib ◽  
A Kumar
Keyword(s):  
Heat Stress ◽  
Blood Cell ◽  
Heat Exposure ◽  
Experimental Period ◽  
Blood Parameters ◽  
Treatment Groups ◽  
Indigenous Sheep ◽  
Significant Difference ◽  
Heat Stress Condition ◽  
Humidity Index

The research was conducted to study the effect of heat stress on blood parameters in indigenous sheep. Nine sheep were divided into three groups which were almost similar in age, sex and weight. Three groups were divided as zero hour (T0), four hours (T4) and eight hours (T8) heat exposure to direct sunlight. During experimental period temperature–humidity index (THI) value was calculated as 27.09 which indicate T4 and T8 groups were subjected to heat stress condition for at least four hours and eight hours respectively every day. The amount of red blood cell (RBC), hemoglobin (Hb%) and packed cell volume (PCV%) were increased significantly (p<0.05) with the increase of heat stress but the amount of white blood cell (WBC) had no significant difference (P>0.05) among the treatment groups. It can be concluded that heat stress had significant changes on some blood parameters in indigenous sheep. DOI: http://dx.doi.org/10.3329/jbau.v12i1.21253 J. Bangladesh Agril. Univ. 12(1): 91-94, June 2014

scholarly journals Reproductive Toxicity and Biomarker Response to a Daily Dose of Tramadol in Male Albino Rats (Rattus norvegicus)

2019 ◽  
pp. 1-7
Author(s):  
E. Oriakpono, Obemeata ◽  
M. Ajah, Ogechi
Keyword(s):  
Treatment Group ◽  
Liver Damage ◽  
Sperm Count ◽  
Control Group ◽  
Albino Rats ◽  
Biomarker Response ◽  
Daily Dose ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

This study was designed to evaluate the effect of a daily dose of tramadol on selected biomarkers viz: haematological parameters, sperm count, kidney and liver damage in male albino rats. Twenty four wistar rats were divided randomly into two groups: control group and treated groups, the treated group were further divided into four groups and housed in cages. Clean drinking water was served to control (group 1), and 1.6 mg/kg bodyweight of tramadol was administered to group 2 (7 days treatment), group 3 (14 days treatment), group 4 (21 days treatment) and group 5 (21 days treatment +7 days withdrawal) in addition to a daily standard diet for all groups. Treatment of rats with tramadol caused significant decrease (P<0.05) in WBC, platelet and lymph. in group 2, on bicarbonate, AST and protein, it showed significant decrease (P<0.05) in group 3, and it showed significant decrease (P<0.05) in group 5 on Cl-, AST, ALT, bicarbonate, AST, PCV, Hb, RBC, WBC, platelet, lymphocytes and sperm count. The results indicates that tramadol has negative effects on the liver which may induce severe liver damage when used for a prolonged period, the results also shows that tramadol can cause anaemia as seen by the observed negative changes in the blood parameters evaluated. Therefore, administration should be with great caution and from a licensed pharmacist or doctor while self prescription or over the counter administration should be avoided considering the associated adverse effects.

scholarly journals Toxicological and Positive Changes in Hematological Pattern of Albino Rats Treated with Termitomyces Titanicus: Potential Valorization of an Indigenous Practice in the Treatment of Oris Cancrum (Noma) in Nigeria

2020 ◽  
pp. 1-9
Author(s):  
Kenneth Yongabi Anchang ◽  
◽  
Augustine Oko ◽  
Seino Richard ◽  
◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Hematological Parameters ◽  
Test Group ◽  
Hemoglobin Level ◽  
Sub Saharan Africa ◽  
Albino Rats ◽  
Vitamin B ◽  
North West ◽  
Significant Difference

A neglected disease affecting many malnourished children and immunosuppressed patients in sub Saharan Africa is noma caused mainly by Fusobacterium necrophorum. Poverty and malnutrition are highly implicated in the epidemiology, prevalence and poor management of noma. Local mushroomsof the gens Termitomyces have been used in parts of northern Nigeria and Cameroon to improve health of noma patients, but detail studies to authenticate the effect of this medicinal mushroom on specific chronic diseases such as noma remain inadequate. Blood transfusion remains one of the critical palliative care strategies to manage anemia in noma disease. The current preliminary study was undertaken to establish the effect of a strain of termitomyces titanicus mushroom, which has been used in Nigerian and Cameroonian folk medicine in Itas Gadau Local government and parts of Bauchi State and North west Region respectively for the management of anemia, using hematological parameters such as hemoglobin level, white blood cell stabilization and packed cell volume of albino rats and also to ascertain its safety for consumption using liver enzyme profiling. The results indicated that rats treated with Termitomyces titanicus had a higher hemoglobin level (12.2 g/dl) compared to a treatment with vitamin B complex (11.3 g/dl) and untreated (10.1 g/dl). A significant increase in the total white blood cell level (26300cells/mm3) was observed with rats treated with mushrooms compared with 7500 cells/mm3 for rats treated with vitamin B complex. Hematological parameters showed a significant difference amongst the test group compared with the controls group. The results further revealed that rats treated with termitomyces had a cleaner liver than with rats treated with vitamin B complex. The results authenticate the practice of using this mushroom in the local management of anemic conditions in Oris cancrum (noma) patients and other chronic disease conditions such as cancer.

scholarly journals RERATA VOLUME TROMBOSIT, HITUNG LEUKOSIT DAN TROMBOSIT DI APENDISITIS AKUT

2018 ◽  
Vol 20 (2) ◽  
pp. 103
Author(s):  
Jayadi Festiawan ◽  
Nurhayana Sennang ◽  
Ibrahim Abdul Samad
Keyword(s):  
Platelet Count ◽  
Retrospective Study ◽  
Acute Appendicitis ◽  
Blood Cell ◽  
Medical Records ◽  
Normal Values ◽  
Platelet Volume ◽  
Blood Tests ◽  
Significant Difference ◽  
The Mean

The diagnosis of acute appendicitis and its complications still depends on the radiological examination and laparoscopy techniques.The aim of this study was to know the Mean Platelet Volume (MPV), WBC and platelet value in the diagnosis of acute appendicitis,associated with the incidence of existing complications. This research was carried out by a retrospective study by taking secondary datafrom the medical records, including the results of routine blood tests of acute appendicitis patients with and without complications atthe Dr. Wahidin Sudirohusodo Hospital between May 2011− May 2012. The data obtained in the study were MPV, White Blood Cell(WBC) and platelet count which were then analyzed using independent T test. The results obtained from 44 samples were that MPVvalues showed a significant difference (p=0.02), while WBC (p=0.12) and platelet count (p=0.13) were statistically not significantlydifferent between patients with and without complications. In conclusion, it was found that acute appendicitis with complications hadhigher MPV average values than those without complications and although there were significant differences between them, these werestill within the normal values.

scholarly journals Haemostatic Alterations after Sildenafil and Tramadol Administration in Rats

2020 ◽  
pp. 153-158
Author(s):  
Evelyn Mgbeoma Eze ◽  
Ransom Baribefii Jacob
Keyword(s):  
Coagulation Factors ◽  
Coagulation System ◽  
Albino Rats ◽  
Platelet Distribution Width ◽  
Platelet Volume ◽  
Extrinsic Pathway ◽  
Distribution Width ◽  
Significant Difference ◽  
Group A ◽  
The Mean

Haemostatic parameters constitute measurable indices in the haemostatic system used to assess the functionality of the coagulation system of an individual to establish a state of health or disorder. This study evaluated haemostatic parameter such as platelets count, mean platelet volume (MPV), platelets distribution width (PDW), prothrombin time (PT) and activated partial thromboplastin time (APTT) in 22 Male Albino Rats grouped and orally treated daily for three weeks with Sildenafil (4 mg/200 g.bwt), Tramadol(6 mg/200 g.bwt) and Sildenafil/Tramadol combination (4+6 mg/220 g.bwt). Rats were sacrificed by cardiac puncture and 5 mls of blood collected for the analysis of the parameters using Sysmex haematology analyser and Agape Diagnostic reagents kits. Results obtained shows a statistically significant increase in platelet count, PT and APTT compared with control across the various groups (p<0.05). A statistically significant decrease was observed in MPV, PDW in Sildenafil+tramadol group, significant decrease in platelets distribution width for Tramadol group when compared with control (p<0.05). No significant difference was observed in the mean platelets volume and platelet distribution width in Sildenafil group. A comparison of Sildenafil+tramadol and Sildenafil groups shows no statistically significant difference in all the parameters analysed. There was also no significant difference in the mean platelets count, PDW, PT and APTT when Sildenafil+tramadol and Tramadol groups were compared (p<0.05). However, a statistically significant increase was seen in platelets count when Sildenafil+tramadol and tramadol were compared (p<0.05). Sildenafil and tramadol causes significant increase in platelets count, prolonged PT and APTT following single/combined daily administration in rats. Further research on these parameters, assessment of liver function, and measurement of intrinsic and extrinsic pathway coagulation factors in human taking this medication is recommended.

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