scholarly journals Phytochemical Screening, GC-MS Analysis and Antioxidant Activity of Three Medicinal Plants From Nigeria

2020 ◽  
pp. 14-26
Author(s):  
Ezekwe Ahamefula S. ◽  
Ordu Kenneth S. ◽  
Oruamabo Ralphael S.
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Retention Time ◽  
Antioxidant Property ◽  
Phytochemical Screening ◽  
Piper Guineense ◽  
Entire Study ◽  
Ms Analysis ◽  
Study Results

Phytochemical screening, GC-MS analysis and antioxidant activity of some medicinal plants were investigated using standard methods. Leaves of Gongronema latifolium, Petrocarpus mildbraedii, and Piper guineense plants were prepared properly and used in the entire study. Results of phytochemical screening for the plants revealed the presence of tannins, saponins, alkaloids, phenolic compounds, reducing sugars amongst others at different concentrations. Results of GC-MS analysis for G.latifolium showed a total of thirty-three compounds of which Cyclohexano, 2-l(methlaminomethyl)-trans had the highest retention time, squalene had the highest molecular weight and phytol had the highest peak area. GC-MS result for P.mildbraedii revealed a total of twenty-three of which Acatamide,2,2,2-trichloro- had the highest retention time, Erucic acid had the highest molecular weight whereas n-Hexadecanoic acid the highest peak area. Only ten compound were observed in P.guineense of  which Ethyl (3-hydroxyphenyl) carbamate had the highest retention time, Benzaminde, N-(4,5-dichloro-1,3-benzothiazol-2yl)-3,5-dimethoxy- had the highest molecular weight and Benzene, 1,2,3-trimethoxy-5-(2-propenyl)- has the highest peak area in P. guineense. The antioxidant activity of the plant extracts as assessed, followed the order P.guineense > G.latifolium> P. mildbraedii in comparative with ascorbic acid used as the control. The observed compounds become very important when the usefulness of these plants as medicinal plants are considered. They could as well be behind the antioxidant property of the plants in this study. This study has revealed the phytochemical screening, GC-MS analysis and antioxidant activity of some medicinal plants.

scholarly journals Phytochemical Screening, GC-MS Analysis and Antioxidant Activity of CurcurbitapepoL. usingIts Leaf Sample

2021 ◽  
pp. 33-45
Author(s):  
Ezekwe Ahamefula Sunday ◽  
Nwadike Constance Nnedimma ◽  
Wokocha Gift Peter ◽  
George Boma Orlando
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Antioxidant Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
State University ◽  
Phytochemical Screening ◽  
Leaf Sample ◽  
Standard Methods ◽  
Ms Analysis ◽  
Ethylhexyl Phthalate

This study evaluated the phytochemical screening, gas chromatography-mass spectrometry (GC-MS) analysis and antioxidant activity of Curcurbitapepo L. using its leaf sample with standard methods. The sample used for the study was procured from Imo State University school farm and was properly identified. Result of phytochemical screening revealed the presence of  saponins, flavonoids, alkaloids, steroids, phlobactannins, proteins, and anthraquinnones, while the GC-MS analysis revealed a total of 78 compounds, out which Bis(2-ethylhexyl) phthalate (C24H38O4) had the highest molecular weight, 2,4,6-Octatriene, 2,6-dimethyl- (C10H16) had the highest peak area of 10.21% while Morphinan-6-ol, 4,5-epoxy-N-methyl-, (5α 6α- (C17H21NO2) had the highest retention time. The antioxidant activity of the studied sample was enhanced against the control. Some of the compounds as revealed by GC-MS analysis could be of healthcare or industrial importance.  There is need for further studies on the leaf sample to ascertain further the observations of the present study. This study has evaluated the phytochemical screening, GC-MS analysis and antioxidant activity of C.pepo L. using its leaf sample.

scholarly journals Phytochemical screening and antioxidant activity of some medicinal plants’ crude juices

2020 ◽  
Vol 28 ◽  
pp. e00536
Author(s):  
Radwan S. Farag ◽  
Mohamed S. Abdel-Latif ◽  
Hanaa H. Abd El Baky ◽  
Layla S. Tawfeek
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Phytochemical Screening

scholarly journals Phytochemical screening and GC MS analysis of methanolic extract of Abelmoschus moschatus

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 3049-3052
Author(s):  
Rani Sebastian ◽  
Jayakar B ◽  
Gomathi V
Keyword(s):  
Methanolic Extract ◽  
Phytochemical Screening ◽  
Cytoprotective Activity ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Acetate Methyl ◽  
Study Results ◽  
Gas Chromatography Mass Spectroscopy ◽  
Vinyl Phenol

The study was designed to evaluate the phytoconstituents present in the methanolic extract of aerial parts of Abelmoschus moschatus. The qualitative phytochemical screening of different extracts of aerial parts revealed the presence of some bioactive compounds. GC – MS analysis was performed using Shimadzu Gas chromatography-mass spectroscopy (Model Number: QP2010S) instrument. GC-MS detection of phytoconstituents was done by computer evaluation of mass spectra of samples through National Institute Standard and Technology (NIST II) and WILEY 8 library. GC – MS analysis detected the presence of 14 compounds. GC – MS profile of the methanolic extract revealed the presence of megastigmatrienone, phytol, loliolide, farnesyl acetate, methyl linoleates, gamma-sitosterol, cis, cis, cis-7,10,13-Hexadecatrienal, thymine, pyranone, coumarin, 2 – methoxy 4 – vinyl phenol, guanosine, chinasaure and 3- cyclopentyl propionic acid 2 dimethyl aminoethyl ester. The current study suggests that methanolic extracts of aerial parts of Abelmoschus moschatus contain phytoconstituents with antioxidant and cytoprotective activity. The study results will pave a way for the production of therapeutic agents which can be used for the treatment of various diseases.

Phytochemical screening and evaluation of antioxidant activity of the stem bark of Anacardium occidentale L. and Vitellaria paradoxa C.F. Gaertn, two medicinal plants from Poro region (Northern of Côte d'Ivoire)

2021 ◽  
Vol 1 (2) ◽  
pp. 022-028
Author(s):  
Gboko Abiba Ouattara ◽  
Tiekpa Wawa Justine ◽  
Kamagaté Tidiane ◽  
Boni Ahoussi Pascal ◽  
Kabran Aka Faustin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Stem Bark ◽  
Anacardium Occidentale ◽  
Phytochemical Screening ◽  
Vitellaria Paradoxa ◽  
Dpph Method ◽  
Treatment Of Diabetes ◽  
Major Chemical ◽  
Chemical Groups

Anacardium occidentale and Vitellaria paradoxa are two medicinal plants traditionally used by Ivorian, particularly in the treatment of diabetes, arterial hypertension and diarrhea. The objective of this work was to evaluate the phytochemical constituents and the antioxidant activity of the stem barks of A. occidentale L. and V. paradoxa. The stem bark of both plants was used for the aqueous extraction. Major chemical groups were identified using the tube staining method followed by confirmation by thin layer chromatography (TLC). The antioxidant activity of the extracts was evaluated by DPPH method. The results of phytochemical screening of plant extracts revealed the presence of polyphenols, anthraquinones, tannins, sterols, terpenes and saponins. In addition, the evaluation of the antioxidant activity by spectrophotometry using the DPPH method showed that all extracts had significant and different antioxydant activity compared to the reference molecule (Vitamin C, IC50 = 0.80 ± 0.70 µg/mL) with respective IC50 of 0.42 ± 0.32 µg/mL for V. paradoxa and 0.87 ± 0.77 µg/mL for A. occidentale. V. paradoxa therefore has the best antioxydant activity. These results could explain the therapeutic virtues of A. occidentale and V. paradoxa in the treatment of various pathologies.

scholarly journals Nigella sativa callus treated with sodium azide exhibit augmented antioxidant activity and DNA damage inhibition

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammed Shariq Iqbal ◽  
Zahra Iqbal ◽  
Abeer Hashem ◽  
Al-Bandari Fahad Al-Arjani ◽  
Elsayed Fathi Abd-Allah ◽  
...  
Keyword(s):  
Dna Damage ◽  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Negative Correlation ◽  
Sodium Azide ◽  
Retention Time ◽  
Nigella Sativa ◽  
Antioxidant Property ◽  
Fresh Weight

AbstractNigella sativa L. (NS) is an herbaceous plant, possessing phytochemicals of therapeutic importance. Thymoquinone is one of the active phytochemicals of NS that confers noteworthy antioxidant properties. Sodium azide, an agent of abiotic stress, can modulates antioxidant system in plants. In the present investigation, sodium azide (0, 5 µM, 10 µM, 20 µM, 50 µM, 100 µM and 200 µM) doses administered to the in vitro NS callus cultures for production/modification of secondary metabolites with augmented activity. 200 µM sodium azide treated NS callus exhibited maximum peroxidase activity (1.286 ± 0.101 nanokatal mg−1 protein) and polyphenol oxidase activity (1.590 ± 0.110 nanokatal mg−1 protein), while 100 µM sodium azide treated NS callus for optimum catalase activity (1.250 ± 0.105 nanokatal mg−1 protein). Further, 200 µM sodium azide treated NS callus obtained significantly the highest phenolics (3.666 ± 0.475 mg g−1 callus fresh weight), 20 µM sodium azide treated NS callus, the highest flavonoids (1.308 ± 0.082 mg g−1 callus fresh weight) and 100 µM sodium azide treated NS callus, the highest carotenes (1.273 ± 0.066 mg g−1 callus fresh weight). However, NS callus exhibited a decrease in thymoquinone yield/content vis-à-vis possible emergence of its analog with 5.3 min retention time and an increase in antioxidant property. Treatment with 200 µM sodium azide registered significantly the lowest percent yield of callus extract (4.6 ± 0.36 mg g−1 callus fresh weight) and thymoquinone yield (16.65 ± 2.52 µg g−1 callus fresh weight) and content (0.36 ± 0.07 mg g−1 callus dry weight) and the highest antioxidant activity (3.873 ± 0.402%), signifying a negative correlation of the former with the latter. DNA damage inhibition (24.3 ± 1.7%) was recorded significantly maximum at 200 µM sodium azide treatment. Sodium azide treated callus also recorded emergence of a new peak at 5.3 min retention time (possibly an analog of thymoquinone with augmented antioxidant activity) whose area exhibits significantly negative correlation with callus extract yield and thymoquinone yield/content and positive correlation with antioxidant activity and in vitro DNA damage inhibition. Thus, sodium azide treatment to NS callus confers possible production of secondary metabolites or thymoquinone analog (s) responsible for elevated antioxidant property and inhibition to DNA damage. The formation of potent antioxidants through sodium azide treatment to NS could be worthy for nutraceutical and pharmaceutical industries.

scholarly journals ANTIOXIDANT ACTIVITY IN COMBINATION EXTRACT OF Acorus calamus L. (DLINGU) AND Allium sativum (GARLIC)

2019 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Pramita Laksitarahmi Isrianto ◽  
Putu Oky Ari Tania
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Experimental Research ◽  
Allium Sativum ◽  
Acorus Calamus ◽  
Test Results ◽  
Phytochemical Screening ◽  
Natural Materials ◽  
Activity Test ◽  
Ic50 Values

One of the efforts to optimize the utilization of natural materials that is used them as medicinal plants, including Dlingu (Acorus calamus L.) and Garlic (Allium sativum) which have the potential as antioxidants. This study aims to determine the effectiveness of a combination extract of Acorus calamus L. (Dlingu) and Allium sativum (Garlic) on antioxidant activity. This research is an experimental research which is the test of antioxidant content and antioxidant activity test with 5 series of concentrations and 4 times of repetition. The research materials, namely dlingu stem and single clove garlic tubers were extracted, phytochemical screening was carried out and then antiradical free IC50 was determined. Based on the test results of the chemical extract of dligu and single clove garlic, the highest content was alkaloid 5.16%, tannin 4.05%, saponin 3.01%, and flavonoid 2.18%. The test results of the chemical antioxidant activity of extracts dligu and single clove garlic contain IC50 values of 17.062 ppm, including a very strong class of antioxidants.Keywords: antioxidant, Dlingu, Single Clove (Male) Garlic.

In vitro Antioxidant, Antibacterial Activities and HPLC-TOF/MS Analysis of Anvillea radiata (Asteraceae) Extracts

2019 ◽  
Vol 15 (4) ◽  
pp. 376-383
Author(s):  
Abderrahim Benslama ◽  
Abdenassar Harrar ◽  
Fatih Gül ◽  
Ibrahim Demirtaş
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Fumaric Acid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Tof Ms

Background:Algeria has a particular geographical position with a wide band of very varied vegetation including the aromatic and medicinal plants. These plants are able to produce much diversified natural compounds, which can be very useful health care and treatment of diseases.Objective:This study was carried out to evaluate the antioxidant capacity and antibacterial activity of Anvillea radiata (Asteraceae) extracts, one of the medicinal plants used as food and in folk medicine in Algeria.Methods:The extraction was carried on the aerial part by methanol and water to give Met.E and Aq.E. The ABTS and DPPH free radicals-scavenging tests are used to evaluate the antioxidant activity of extracts. Moreover, the antibacterial activity was evaluated using disc diffusion method. Finally, the extracts were subject to the phytochemical analysis using the HPLC-TOF/MS equipment.Results:The Met.E showed the highest amount of total phenolic and flavonoids content (266.12 µg EAG/mg E and 50.83 µg EQ/mg E, respectively). The results of the antioxidant activity revealed that the Met.E showed a good scavenging activity against ABTS and DPPH free radical with EC50=0.067 mg/ml and EC50=0.33 mg/ml, respectively. Moreover, the A. radiata extracts present a highest antibacterial activity against M. luteus NRRL B-4375, B. subtilis NRRL NRS-744 and S. aureus ATCC 25923, with inhibition zone ranging from 18 to 23 mm to Met.E and 12-18 mm to the Aq.E. In addition, the Met.E of A. radiata showed a better MIC against S. aureus, M. luteus and B. subtilis (MIC= 6.25, 25 and 25 µg/ml, respectively). The HPLC-TOF/MS analysis of Met.E reveals the presence of two major compounds, rutin and fumaric acid, when Aq.E contains the syringic acid as a major compound.Conclusion:The data of our study reported that the Met.E extract of A. radiata had a considerable antioxidant capacity and antibacterial effect, which may be due to their richness by phenolic compounds such as rutin and fumaric acid.

EVALUATION OF ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF HARDWIKIA BINATA (ROXB.)

2021 ◽  
pp. 256-258
Author(s):  
Varsha D. Hutke ◽  
Vaishali Rathod
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Secondary Metabolites ◽  
Aqueous Extract ◽  
Ethanol Extract ◽  
Single Species ◽  
Antioxidant Property ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
The Family

The genus Hardwickia of the family Caesalpiniaceae is represented by a single species Hardwickia binata Roxb. The present study deals with screening of secondary metabolites and antioxidant activity of different extracts of leaf and bark of Hardwickia binata. Phytochemical screening revealed the presence of various secondary metabolites viz. alkaloids, saponins, phenolics compound, tannins, phenols and steroids. Both leaf and bark extracts scavenged free radicals signicantly. Whereas aqueous extract of bark and ethanol extract of leaf exhibited good antioxidant property

scholarly journals Phytochemical Screening and Antioxidant Activity of some Egyptian Medicinal Plants

2022 ◽  
Vol 4 (1) ◽  
pp. 14-20
Author(s):  
Heba sayed ◽  
mohammady issa ◽  
magda mahmoud ◽  
emad abdelkader ◽  
hamadi ismail
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Phytochemical Screening

scholarly journals Phytochemistry and Antioxidant Activity of Irvingiagabonensis (Bush mango) Seed Sample

2021 ◽  
pp. 25-34
Author(s):  
Ezekwe Ahamefula Sunday ◽  
Nwadike Constance Nnedimma ◽  
Wokocha Gift Peter ◽  
George Boma Orlando
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Reducing Sugars ◽  
Gas Chromatography Mass Spectrometry ◽  
Seed Sample ◽  
Phytochemical Screening ◽  
Ms Analysis ◽  
Bush Mango ◽  
Medicinal Food ◽  
Mango Seed

Phytochemistry and antioxidant activity of Irvingiagabonensis (Bush mango) seed sample were evaluated. Dried milled seeds of I.gabonensis popularly known as “ogbono” passed through phytochemical screening, Gas chromatography-mass spectrometry (GC-MS) analysis and antioxidant studies. Tannins, saponins, flavonoids, alkaloids, steroids, and reducing sugars were among the phytochemicals found present after screening. About 20 constituents were revealed by the GC-MS analysis out of which 1,3-O-Benzylidine glyceryl-2-myristate had the highest molecular weight, 2-Undecanone had the highest peak area of 45.39% whereas 3-Dibenzofuranamine had the highest retention time. Some of these constituents could have one or two applications in medicinal, food or cosmetic industries. I.gabonensis seeds showed better antioxidant activity against the control at concentrations considered in this study. Some of these constituents as well could be behind the observed antioxidant activity. This study has evaluated the phytochemistry and antioxidant activity of I.gabonensis (Bush mango) seed sample.

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