Effect of Replacing Bone Ash with Fresh Water Snail (Pila Ampullacea) Shell Ash on Serum Biochemical Indices of Weaned Rabbits

Author(s):  
F. B. P. Abang ◽  
S. S. Emmanuel ◽  
S. Attah

Twenty five (25) mixed breed weaned rabbits were utilized in 84-days experiment to investigate the effect of replacing bone ash with fresh water snail (Pila ampullacea) shell ash on the serum biochemical composition of weaned rabbits. The experiment was conducted at the Teaching and Research Farm, University of Agriculture, Makurdi, Benue State, Nigeria for a period of 12 weeks. The rabbits were randomly assigned to the five dietary treatments and replicated five times giving a total of one rabbit per replicate in a completely randomized design (CRD).   Rabbits were raised intensively in hutches of about 4ft × 4ft. Five experimental diets tagged T1 to T5 were formulated  such that fresh water snail (Pila ampullacea) shell ash replaced bone ash at 0%, 25%, 50%, 75% and 100%. Feed and water were served ad libitum. Bio sanitary and bio security measures were strictly adhered to. At the end of the feeding trial, three rabbits per treatment were selected for the evaluation of serum biochemistry. The parameters assayed for were: total serum protein, serum globulin, serum albumin, cholesterol, serum calcium, and serum phosphorus. The study showed that the serum biochemical indices were not influenced (P˃0.05) by the dietary treatments except for calcium which was significantly (P<0.05) influenced, however, calcium values were within the normal reference values for rabbits. This study had shown that fresh water snail (Pila ampullacea) shell ash can serve as a substitute for bone ash in weaned rabbit diet up to 100% inclusion levels without adverse effect on the serum biochemical indices.

Author(s):  
F. B. P. Abang ◽  
S. S. Emmanuel ◽  
S. Attah

Twenty five weaned male rabbits of mixed breeds were used to investigate the effect of replacing bone ash with fresh water snail shell ash on the performance of weaned rabbits. The experiment was conducted at the Teaching and Research Farm, University of Agriculture, Makurdi, Benue State, Nigeria for a period of 12 weeks. The rabbits were randomly assigned to the five dietary treatments and replicated five times giving a total of one rabbit per replicate in a completely randomized design (CRD). Rabbits were raised intensively in hutches of about 4ft × 4ft. Five experimental diets tagged T1 to T5 were formulated such that fresh water snail shell ash replaced bone ash at 0%, 25%, 50%, 75% and 100%. Feed and water were served ad libitum. Bio sanitary and bio security measures were strictly adhered. The study showed that the growth parameters were not affected (P=.05) by the dietary treatments. This study had shown that fresh water snail shell ash can serve as a substitute for bone ash in weaned rabbits diet up to 100% without compromising their performances.


2020 ◽  
Vol 47 (4) ◽  
pp. 167-175
Author(s):  
I. B. Amaza ◽  
A. Maidala ◽  
C. E. Isidahomen

Haematological and serum biochemical parameters are indicators of physiological, nutritional and pathological status of animals. Investigation of these parameters provide information for diagnostic purposes. The study was conducted to determine the influence of feeding graded levels of yam peel meal (YPM) as a replacement for maize on haematology; blood chemistry was evaluated in rabbits. Thirty- six (New Zealand white X Chinchilla) were randomly assigned to four dietary treatments in a completely randomized design with nine rabbits per treatment. The rabbits were fed diets containing 0, 12.5, 25 and 37.5% of Yam peel meal (YPM) as a replacement for maize, designated as T1, T2, T3 and T4, respectively. The experimental diets and clean drinking water were supplied ad libitum throughout the experimental period of nine weeks. At the end of the feeding trial, three rabbits per treatment were randomly selected for hematological and serum biochemical analysis. All the haematological and serum biochemical indices except AST were affected (P<0.05) by dietary inclusion of YPM. Although most of the parameters investigated were affected by rabbits fed test ingredient, the values fell within the normal literature reference values for rabbits. It can therefore be concluded that up to 37.5% inclusion level of YPM can be fed to growing rabbits without deleterious effects on health status.


2021 ◽  
Vol 44 (3) ◽  
Author(s):  
A. F Agboola

Since the cost of feed is increasing due to the keen competition between humans and animals, there is a need to critically seek for a matching alternative. Castor seed cake, a residue after the extraction of oil from nutrients-rich castor seed constitutes a waste which can be converted into livestock feed. Thus, the study was carried out to investigate the effect of graded levels of castor seed cake diets on haematological and serum biochemical indices of weanling albino rats in an experiment that lasted 21 days. Thirty weanling Wistar albino rats were weighed individually and randomly assigned to 5 dietary treatments with 3 replicates of two rats in a completely randomized design. Diet 1 was a nitrogen-free diet (NFD), while diets 2, 3, 4, and 5 had 0% (without castor seed cake inclusion), 2, 4 and 6% castor seed cake replacing soyabean meal respectively. At day 21, blood samples were obtained from two rats per replicate for haematological and serum analysis. There were no significant differences (P>0.05) observed in all the parameters (packed cell volume, haemaglobin, red blood cell, white blood cell, lymphocyte, neutrophil, monocyte, eosinophil and platelet) measured. Similarly, diet had no effect on the serum biochemical indices (creatinine, urea, total protein and globulin) measured. Significant difference (P<0.05) was however observed in albumin (2.62 – 3.18g/dL) of rats on the different experimental diets. The albumin concentrations of weanling albino rats on dietary treatments were similar to those on the control diet but significantly (P<0.05) higher (3.09 – 3.18g/dL) than those on the nitrogen free diet. It can be concluded that castor seed cake can replace soyabean meal up to 6% inclusion level without eliciting detrimental effect on blood profile of weanling wistar albino rats.


1971 ◽  
Vol 17 (4) ◽  
pp. 301-306 ◽  
Author(s):  
John Savory ◽  
M Geraldine Heintges ◽  
Robert E Sobel

Abstract An automated continuous-flow procedure has been developed for simultaneously measuring total serum protein and globulin. The method for total protein is a minor modification of an existing automated method in which the biuret reagent is used. Total globulin is measured by reaction with glyoxylic acid, and standardized with N-acetyltryptophan. An empirical factor relating concentration of N-acetyltryptophan to human globulin has been derived. Values for total serum globulin obtained by this new automated procedure correlate closely with values obtained by electrophoresis, but do not agree with values obtained by use of procedures involving binding of anionic dye. Recovery of gamma globulin added to serum is essentially quantitative; the day-to-day precision (CV) is 4.31%.


2018 ◽  
Vol 39 (4) ◽  
pp. 1819
Author(s):  
Antonio Diego Brandão Melo ◽  
Angela Oliveira ◽  
Geovane Rosa De Oliveira ◽  
Chiara Domingues ◽  
Kelly Mazutti ◽  
...  

An experiment was conducted to evaluate the toxic effects of short-term ingestion of a zearalenone, fumonisin, and aflatoxin mixture, at low concentration, into the diet of weanling piglets and to assess the protective efficacy of an anti-mycotoxin feed additive. For 21 days, piglets were exposed to control or multi-mycotoxin treatment with or without the anti-mycotoxin additive. Growth performance was measured after 21d. Blood samples were taken to serum biochemical analysis and for quantify levels of circulating mononuclear immune cells. Effects on organs weights and histological changes, and expression levels of COX-2 and TNF-? in the liver and jejunum were evaluated. Overall, the multi-mycotoxin treatment had no effect on measured variables, and no adverse histopathological changes in organs were observed. The total serum protein concentration was higher in animals that received the multi-mycotoxin treatment; however, levels remained within normal limits for weanling piglets. In conclusion, the short-term multi-mycotoxin mixture, at the dose levels evaluated in this study, seems not affect the health of weanling piglets through the evaluated parameters. The absence of toxicity associated with the multi-mycotoxin treatment rendered it impossible to evaluate the efficacy of the anti-mycotoxin additive.


2016 ◽  
Vol 50 (6) ◽  
Author(s):  
S. Khanna ◽  
H. K. Gulati ◽  
S. Kumar ◽  
P. K. Kapoor

The effect of Emblica officianalis and Spirulina platensis was studied in New Zealand White rabbits. Weaned rabbits of both sex were allocated to three different feeding treatments via T1 (Control), T2 (supplemented with 2% Emblica officianalis) and T3 (supplemented with 5% Sprulina platensis) in addition to ad lib. berseem fodder. The concentrate diets formulated were iso-nitrogenous and iso-caloric. The final body weight, weight gain and average daily weight gain of rabbits in all the groups followed the same trend. While, T1 and T2 were found to have better FCR than T3 supplemented feeding treatments, there were no significant changes in the serum biochemical indices, only the serum globulin value was significantly (P less than 0.05) lower (2.41 vs. 3.55, 3.10 g/dl) and albumin value was found significantly higher (4.52 vs. 3.27, 3.56 g/dl) in the groups fed spirulina supplemented diets as compared to groups fed with non supplemented diets. Amla supplementation had no effect on the serum biochemistry indices.


2020 ◽  
Vol 47 (3) ◽  
pp. 59-66
Author(s):  
H. O. Obikaonu ◽  
S. N. Obiora ◽  
P. Obiagwu

Attempts made to improve broiler production had been focused more on feed with little or no consideration on the impact of litter quality which is a major concern in chicken production not only because it affects the flock health and productivity, but also because it may act as potential reservoir and transmission vehicle for pathogens and potential pathogens. Since blood is considered as an important material for the evaluation of health status in animals, there is need therefore, to investigate the impact of different litter materials on the hematological and serum biochemistry of broiler finisher chicken. A56-day feeding trial was conducted to evaulate the effect of different litter materials on the haematological and serum biochemical indices of intensively managed finisher broilers. Four different litter materials were used for the experiment viz wood, shaving, Groundnut hulls; coconut husks and rice hulls. Wood shavings was used as the control owing to the fact that it is the most commonly used litter in this zone. The coconut husks were manually shred to extract the fibres which was used while the other materials were used as sourced. A toal of 120 broiler birds were used for the experiment, they were divided into four treatments (T1= Wood shavings, T2= Groundnut hulls, T3=Coconut husks, and T4=Rice hulls) of 30 birds each and sub divided into 10 birds per replicate. Each replicate was housed in a pen fitted with broding facilities. Feed and water were given ad libilum for eight weeks. At the end of the experiment blood was collected from the birds, three per treatment given a total of 12 birds. They were analyzed for haematological and serum biochemical indices. Haemoglobin values compared favourably (P>0.05) with that of the control. PCVvalues of T2 and T3 compared favourably well with that of control but significant differences existed (P<0.05) between the PCV value of T4 and the other treatments. The lymphocytes and neutrophils were not affected by the bedding materials showing that the litter conditions did not adversely affect the health and welfare of the birds. Serum protein, serum albumin and serum globulin were not affected by treatements (P>0.05) resulting in better protein efficiency ratio recorded in all the treatments. Serum electolytes calcium and phosphorus levels recorded in this study shows no adverse effect on the integrity of the liver in boosting cataion/anaion exchange. The haematological and serum biochemical indices recorded in this study showed no notable health challenges on the birds.


2015 ◽  
Vol 1 (2) ◽  
pp. 259-264 ◽  
Author(s):  
Md Samun Sarker ◽  
Md Ahaduzzaman ◽  
Md Abu Sayeed ◽  
Rajib Sarker ◽  
Minhazul Abedin Nanno ◽  
...  

Physiological status-related changes in serum biochemical values are known to occur in many species. The aim of the current study was to evaluate total serum protein, albumin, glucose, calcium and phosphorus concentration and these parameters were measured by the serum analyzer for lactating (n=50) and non-lactating (n=50) dairy cattle in selected dairy farms of Chittagong district. The results (mean ± standard deviation) of the study showed that among serum biochemical parameters total protein (82.3 ± 2.15 gm/l), albumin (27.45 ± 5.06 gm/l), calcium (12.97±6.67 mg/dl) and phosphorus (7.26 ± 0.41 mg/dl) level in non-lactating cows were higher than the value of lactating cows as (81.28 ± 2.41 gm/l), (14.09 ± 2.05 gm/l), (10.05 ± 2.51 mg/dl) and (6.52 ± 0.30 mg/dl) respectively. Only glucose level in lactating cows (63.01 ± 6.67 mg/dl) was found higher than the non lactating cows (58.47 ± 3.84 mg/dl). In case of parity-wise parameters insignificant variation was noticed. It may be concluded that biochemical variation present between lactating cow and non lactating cow is important for interpretation of laboratory data and also for physiological diagnosis.Asian J. Med. Biol. Res. June 2015, 1(2): 259-264


Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2181
Author(s):  
Silvia Martínez-Miró ◽  
Josefa Madrid ◽  
Miguel José López ◽  
Juan Orengo ◽  
Cristian Jesús Sánchez ◽  
...  

A total of 192 Iberian × Duroc pigs kept under intensive conditions were used to investigate the effects of feeding crude glycerin on growth performance, nutrient digestibility, and blood parameters. Animals were blocked by initial body weight (96.0 ± 11.3 kg) and allotted to pens (16 pigs per pen). Pens were assigned randomly to one of three dietary treatments (four pens per treatment). Dietary treatments contained 0, 5, or 10% of crude glycerin proportionally substituting for wheat (G0, G5, and G10, respectively). Diets were formulated to be isoenergetic and isoaminoacidic. No significant effect of crude glycerin was observed on average daily gain, average feed intake, or feed conversion ratio. The apparent total tract digestibility of dry matter, organic matter, and crude protein was no different between treatments. Total serum protein, albumin, glucose, insulin, and IGF-1 were not affected by glycerin inclusion. In conclusion, crude glycerin up to 100 g/kg can be included in the diets of finishing Iberian crossbred pigs without any negative effect on growth performance, digestibility, or serum metabolic parameters.


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