scholarly journals Phytochemical Activities of Artemissia absinthium and Butea monosperma Plant Extracts

2021 ◽  
pp. 1-7
Author(s):  
Fatima Waheed ◽  
Shaista Yaqoob ◽  
Qurban Ali ◽  
Arif Malik
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Plant Extracts ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Antimicrobial Drugs ◽  
Butea Monosperma ◽  
Fungal Strains ◽  
Inhibition Zones ◽  
Agar Well Diffusion Method

With the developing time, there is a tremendous need to deliver highly potential antimicrobial drugs and treatment from different herbaceous plants because plants are now days commonly used to provide resistance against disease-causing pathogens. This study has been done by using agar well diffusion method. The bacterial along with fungal strains were collected and were cultured on agar plates. Afterwards, these plates were put in incubate for 24 hours at 370C temperature. Within this duration, the zones were developing all around the plates, wherever plant extracts were poured. The activities of microorganisms were measured by using inhibition diameter zones. The inhibition zones were shown clearly on petri plates. Antimicrobial activities of plant extracts of Artemissia absinthium and Butea monosperma tested against bacterial and fungal strains viz. Pseudomonas aeruginosa, Bacillus subtilis and Escherichia coli as well as fungal strains were also used viz. Aspergillus niger and Fusarium oxysporum.

scholarly journals PENGARUH PEMANASAN, TINGKAT KEASAMAN (pH) DAN KONSENTRASI GARAM TERHADAP STABILITAS ANTI BAKTERI EKSTRAK ETANOL KULIT KAYU AKWAY (Drimys piperita Hook f.)

2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Isak Silamba ◽  
Nitia Nilawati ◽  
Eka Syartika
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Inhibitory Concentration ◽  
Heating Time ◽  
Diffusion Method ◽  
Ethanol Extracts ◽  
And Staphylococcus Aureus ◽  
Agar Well Diffusion Method

ABSTRACT  Akway (Drimys piperita) is a woody, evergreen and aromatic plant that belongs to family winteraceae. This plant is used by Sougb tribe lived in Sururey village, District of Anggi, to enhance the vitality of body. The objectives of the research were to determine antimicrobial stability of akway bark extracts influenced by heating time of 100OC, levels of acidity (pH) and salt contents.. Antimicrobial assays were done by using agar well diffusion method against four species of bacteria, i.e.  Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. The results showed that ethanolic extracts of akway bark only inhibited growth of B. cereus and S. aureus with minimum inhibitory concentration 0,99% and 0,89% . The levels of concentration and acidity of ethanol extracts  influenced the antimicrobial capacity of extracts.. Whereas heating time on 100OC during 25 minutes and salt contents  up to 5% of extract solution did not influence the antimicrobial stability of  akway bark extracts. Key words : akway, extracts, antimicrobe, pH, Heating, salt ABSTRAK Akway (Drimys piperita) adalah tumbuhan berkayu, aromatik dan hijau sepanjang tahun dan tergolong dalam suku winteraceae. Tumbuhan ini digunakan oleh Suku Sougb yang bermukim di desa Sururey Distrik Anggi, untuk mengobati malaria dan meningkatkan vitalitas tubuh. Tujuan dari penelitian ini adalah untuk menentukan stabilitas antimikroba ekstrak kulit kayu akway pada waktu pemanasan ekstrak pada 100OC, tingkat keasaman (pH) dan kandungan garam. Pengujian antimikroba dilakukan dengan menggunakan metode difusi agar terhadap empat spesies bakteri yaitu Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. Hasil menunjukkan bahwa ekstrak etanol kulit kayu akway hanya dapat menghambat bakteri Bacillus cereus dan Staphylococcus aureus dengan konsentrasi hambat minimum masing-masing adalah 0,99% dan 0,89%. Tingkat konsentrasi dan keasaman (pH) mempengaruhi kapasitas antimikroba ekstrak etanol kulit kayu akway. Sedangkan perlakuan pemanasan pada suhu 100OC dengan lama pemanasan sampai dengan 25 menit dan penambahan garam NaCl sampai konsentrasi 5%  tidak berpengaruh pada stabilitas antimikroba ekstrak etanol kulit kayu akway.  

scholarly journals Evaluation of antimicrobial potential and phytochemical screening of Moringa oleifera leave extract: An Emerging folkloric medicinal plant in Maiduguri, Borno state, Nigeria

2020 ◽  
Vol 4 (3) ◽  
pp. 043-047
Author(s):  
Fatima Adamu Lawan ◽  
Binta Baba Shehu ◽  
Ali Abba Gana Benisheikh ◽  
Abba Mohammad Dige ◽  
Hassan Arabi Jidda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Medicinal Plant ◽  
Moringa Oleifera ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Antimicrobial Potential ◽  
Agar Disc

The aim of this study was to evaluate the antimicrobial potential and phytochemical screening of Moringa oleifera leave extract as folkloric medicinal alternative. The phytochemical screening result revealed the presence of Alkanoids, Saponins, Fats & oil, Terpenoids and Tanin in all the solvents used for the study. Agar-disc diffusion method were used to determine the antimicrobial activities of the Moringa oleifera extracts on microorganisms. Maximum activities of inhibition were recorded on Ethyl acetate (50mg/ml) against Pseudomonas auroginusa, moderarate activities against Escherichia coli. Likewise 50mg/ml of Haxane extract show weak activities on Pseudomonas aeruginosa, moderate in both Staphalococcus aureus and Escherichia coli. Whereas 50mg/ml of Methanol show moderate activity on Pseudomonas aeruginosa, Staphalococcus aureus and weak activities on Escherichia coli respectively. The research revealed that Moringa oleifera bioactive components would be a safer alternative for curative and preventive emerging folkloric medicinal plant for treatment of bacterial ailments in Maiduguri, Borno state, Nigeria.

scholarly journals SYNTHESIS AND ANTIMICROBIAL PROFILE OF SOME NEWER HETEROCYCLES BEARING THIAZOLE MOIETY

2012 ◽  
Vol 20 (20) ◽  
pp. 61-67
Author(s):  
RAJUL GUPTA ◽  
Neeraj Kumar FULORIA ◽  
Shivkanya FULORIA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Acetic Anhydride ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Disk Diffusion Method ◽  
Antibacterial And Antifungal

Various substituted acetophenones on treatment. with iodine and thiourea yielded 2-amino-4-( substituted-phenyl)-thiazole, which on further treatment with acetic anhydride generated N-(4-(substituted phenyl)thiazol-2-ylacetamide(1-5). All the synthesized compounds were characterized by their respective FTIR, 1H NMR, and mass data. Synthesized compounds (l, 2, 3, 4, 5) when subjected to investigation for their antimicrobial activities i.e. antibacterial and antifungal studies against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Aspergillus flavus, and Aspergillus fumigatus by disk diffusion method, revealed that compound 2 deemed to be most potent with the largest zone of inhibition.

scholarly journals Phytochemical Activities of Artemissia vulgaris and Acacia nilotica Plant Extracts

2021 ◽  
pp. 66-73
Author(s):  
Shaista Yaqoob ◽  
Fatima Waheed ◽  
Qurban Ali ◽  
Arif Malik
Keyword(s):  
Plant Extracts ◽  
Multiple Drug Resistance ◽  
Antimicrobial Activities ◽  
Acacia Nilotica ◽  
Diffusion Method ◽  
Multiple Drug ◽  
Bacterial Strains ◽  
Antimicrobial Drugs ◽  
Water Extracts ◽  
Bacteria And Fungi

With the growing era, there is tremendous need to produce high potential antimicrobial drugs and medicines from various herbaceous plants and natural resources has been increasing due to increasing multiple drug resistance in pathogens. An experiment was performed to know about antimicrobial activities of various plant extracts. These activities were tested by using agar diffusion method. The bacterial as well as fungal strains were collected and were cultured on agar plates. After that these plates were left in incubator for 24 hours at 37°C to develop zones clearly all round the plant extracts. The activities of bacteria and fungi were determined by using inhibition diameter zones. The clear inhibition zones were found against bacterial strains in study. Antimicrobial activity of acetone, n-hexane and water extracts viz. Acacia nilotica and Artemissia vulgaris tested against Pseudomonas aeruginosa, Bacillus subtils and E. coli as well as Aspergillus niger and Fusarium oxysporum. Acetone extracts were found to be more effective as compared with n-hexane while water extracts show no activity.  

scholarly journals MOMORDICA CHARANTIA L. (bitter gourd) AS A CANDIDATE FOR THE CONTROL OF BACTERIAL AND FUNGAL GROWTH

2019 ◽  
Vol 56 (04) ◽  
pp. 1031-1036
Author(s):  
M. Shahid Mahmood
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Cost Effective ◽  
Salmonella Typhi ◽  
Bitter Gourd ◽  
Penicillium Expansum ◽  
Diffusion Method ◽  
Zones Of Inhibition ◽  
Agar Well Diffusion Method

The plants use for medicinal purposes have been experienced by an extensive proportion of population of population for many centuries. The present study was designed to formulate cost effective dietary interventions to prevent and treat certain microbial diseases. The current experiment was conducted for evaluation of antifungal and antibacterial effects of bitter gourd (Momordica charantia L.), which is locally named as Karella. All parts of bitter gourd were used including seeds, pulp and skin. Its methanolic extracts were taken to check its antibacterial efficacy against Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli and methicillin resistance Staphylococcus aureus (MRSA) by paper disc diffusion test. Antifungal efficacy against selected species of Penicillium and Aspergillus was done by Agar Well-Diffusion Method. Broth Dilution method and Agar Well-Diffusion Method were used to determine the minimum inhibitory concentration (MIC). Zones of inhibition diameters were 11mm, 7mm, 11mm and 9mm for Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa and Escherichia coli, respectively. There was no zone of inhibition against MRSA. The zones of inhibition for bitter gourd seed were 12mm, 8mm, 12mm and 11mm for Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi, respectively. The MIC of bitter gourd pulp against Aspergillus niger and Penicillium expansum was 400mg/ml and 600mg/ml, respectively. The MIC of bitter gourd seed against Aspergillus niger and Penicillium expansum was 300mg/ml and 400mg/ml, respectively. Taking together, bitter ground was found a cost effective antibacterial and antifungal agent, it may be used an effective alternate remedial option

scholarly journals Antibacterial Activity of Selected Ethnomedicinal Plants Popular in Magar Ethnic Community of Palpa District, Western Nepal

2021 ◽  
Vol 3 (1) ◽  
pp. 13-19
Author(s):  
Bimala Pangeni ◽  
Shandesh Bhattarai ◽  
Hari Paudyal ◽  
Ram Chaudhary
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Plant Extracts ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Positive Effects ◽  
Medicinal Plant Extracts

The main objective of this research was to explore the potential antibacterial activity of 25 selected medicinal plant extracts against four strains of bacteria. The ethnomedicinal knowledge was documented using semi-structured, open-ended questionnaires, informal interviews, and group discussions with traditional healers and knowledgeable persons about plants and plant-based remedies. The evaluation of antibacterial activities of twenty-five extracts of different plants was carried out by adopting the disc diffusion method for four bacterial strains, namely - Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus. The results were reported by observing the inhibition zones. The results indicated that among 25 plant extracts examined, 16 (64%) plant extracts showed antibacterial property against Escherichia coli followed by 15 (60%) plant extracts against Pseudomonas aeruginosa, and 19 (76%) extracts each against Staphylococcus aureus and Bacillus subtilis. Four plant extracts were not able to produce the zone of inhibition with any of the tested bacteria. Gram-positive bacteria are found to show more positive effects as compared to Gram-negative. Present findings of this study indicate that ethnomedicinal plant extracts have antibacterial activity against the different strains of tested bacteria. This activity supports their use in the treatment of infections caused by such resistant bacteria.

Green synthesis, characterization and antimicrobial activities of Copper nanoparticles from the rhizomes extract of Picrorhiza kurroa

2021 ◽  
Vol 09 ◽  
Author(s):  
Vinit Prakash ◽  
Anjana Kumari ◽  
Harpreet Kaur ◽  
Manoj Kumar ◽  
Sumeet Gupta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Copper Nanoparticles ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Spectral Studies ◽  
Blue Color ◽  
Picrorhiza Kurroa ◽  
Fungal Strains ◽  
Gastrointestinal Problems ◽  
Hydroethanolic Extract

Background: Green synthesized nanoparticles from the solvent extract of various plant parts show better biological activities as compared to parent solvent plant extract. Traditionally rhizomes of Picrorhiza kurroa are used to cure various diseases like diarrhea, fever, jaundice, eye infection, skin problems, asthma, arthritis, cancer, diabetes, gastrointestinal problems. Objectives: Present study describes the synthesis of copper nanoparticles from a hydroethanolic extract of P. kurroa rhizomes (CuNPs-Pk) and their evaluation for antimicrobial activities against gram-negative, gram-positive bacterial, and fungal strains. Methods: The solution of copper sulfate and hydroethanolic extract of rhizomes of P. kurroa was mixed with help of a magnetic stirrer at 60°C temperature for 1 h. The blue color of CuSO4.5H2O changed to brownish-black colored copper nanoparticles within 10 minutes. These nanoparticles were centrifuged at 4000 rpm for 20 min, washed with ethanol, followed by deionized water, dried, and were characterized by Ultra violet–visible (UV-Vis) absorption spectra, Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (TEM). Different concentrations of hydroethanolic extract of Picrorhiza kurroa rhizomes (HEEPk), CuNPs-Pk and copper oxide nanoparticles (bare CuO) ranging from 100-400 ppm had been studied against selected bacterial and fungal strains by using the well plate diffusion method. Ciprofloxin and fluconazole were used as standard and Dimethyl sulfoxide (DMSO) as a control for selected strains. Results: The UV–Vis spectral studies confirmed the surface plasmon resonance of green-synthesized CuNPs-Pk. The particle size was found to be 275-285 nm. FTIR analysis of biosynthesis nanoparticles have been confirm the presence of various functional groups (flavonoids, glycosides, tannins, phenols). SEM and TEM of biosynthesized nanoparticles have predicted their spheric al shape and their size (20-40 nm) and These particles have shown effective antimicrobial activities against selected pathogenic organisms viz. Escherichia coli, Staphylococcus aureus, and Aspergillus niger than that of HEEPk and bare CuO. Conclusions: The CuNPs-Pk show effective antimicrobial activities against bacterial and fungal pathogens as compared to HEEPk and bare CuO.

scholarly journals ANTIFUNGAL AND ANTIBACTERIAL ACTIVITY OF ALOE VERA PLANT EXTRACT

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
P Danish ◽  
Q Ali ◽  
MM Hafeez ◽  
A Malik
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Aspergillus Niger ◽  
Bacillus Megaterium ◽  
Aloe Vera ◽  
Water Soluble ◽  
Root Extract ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Fungal Strains

Aloe vera is a well-known medicinal plant used in many therapeutic purposes. Naturally it is composed of many useful compounds that have ability to use for treatment of many diseases. The active compounds reported in this plant are saponins, sugar, enzymes, vitamins, aloesin, aloeemodin, aloin, acemannan aloemannan, aloeride, methylchromones, flavonoids, naftoquinones, sterols, minerals, anthraquinones, amino acids, lignin and salicylic acid and other different compounds including fat-soluble and water-soluble vitamins, enzymes, minerals, simple/complex sugars, organic acid and phenolic compounds. In this study aloe vera is used for antibacterial and antifulgal activity against different strains of bacteria and pathogenic fungal strains. Ethanol extract of Aloe vera leaves and roots is applied on these bacterial and fungal strains in different concentrations (15, 20, 25, 30µl). Bacillus cereus, Bacillus subtitis, Bacillus megaterium, Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, and some other bacterial strains are used for this study. Escherichia coli and Agrobacterium tumefacins shows zone of inhibition around 18mm which consider as good result. Bacillus subtitis and Bacillus megaterium also shows good result around 16mm. Proteus mirabilis and Pseudomonas aeruginosa shows minimum zone of inhibition which is around 11mm. among all used fungal strains (fuserium oxysporum, Candida albicans, Aspergillus fumigatus, Aspergillus niger) fuserium oxysporum and Aspergillus niger shows excellent results around 19mm both against root extract and leaves extract.

scholarly journals ANTIMICROBIAL ACTIVITY OF SELECTED INDIAN FOLK MEDICINAL PLANTS: MYRISTICA FATUA, ALSTONIA BOONEI, HELICTERES ISORA, VITEX ALTISSIMA AND ATALANTIA RACEMOSA

2017 ◽  
Vol 10 (2) ◽  
pp. 277
Author(s):  
Kokkaiah Irulandi ◽  
Sethupandian Geetha ◽  
Palanichamy Mehalingam
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Alternative Source ◽  
Helicteres Isora ◽  
Acetone Extracts

Objective: To determine antimicrobial activity of methanol, ethyl acetate and acetone extracts of Myristica fatua, Alstonia boonei, Helicteres isora, Vitex altissima and Atalantia racemosa  against different species of pathogens, Streptococcus feacalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Staphyllococcus aureus, and Candida albicans.Methods: Antimicrobial activity of plant extracts was measured by agar well diffusion method.Results: Acetone extracts of Alstonia boonei showed the highest inhibitory effect against Escherichia coli (21.00 ± 1.00 mm) and Streptococcus faecalis (19.00 ± 1.00 mm). All the extracts of Heliteres isora leaves showed different zone of inhibition observed in all the tested pathogens ranges between (8.13 ± 1.53 – 15.25 ± 1.23 mm). Ethyl acetate extract of Vitex altissima showed highest activity against Bacillus sublilis (19.67 ± 1.53 mm). Methanol and acetone leaves extracts of Atalantia racemosa have good fungal activity against the Candida albicans (19.33 ± 1.26 mm - 16.00 ± 1.00 mm). Methanol extract of Myristica fatua showed high antimicrobial activity against Pseudomonas aeruginosa (15.10 ± 0.17 mm) and Bacillus subtilis (14.23 ± 0.21 mm).Conclusion: The results from the study suggest that the leaves Myristica fatua, Alstonia boonei, Helicteres isora, Vitex altissima and Atalantia racemosa showed good antimicrobial activity against the different pathogens. They are used as the alternative source for the control and treatment of microbial infections.Keywords: Antimicrobial activity, Leaves extracts,  Well diffusion method, Pathogenic strains.

scholarly journals A New 3-Prenyl-2-flavene and Other Extractives from Baphia massaiensis and Their Antimicrobial Activities

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Ngonye Keroletswe ◽  
Runner R. T. Majinda ◽  
Ishmael B. Masesane
Keyword(s):  
Escherichia Coli ◽  
2D Nmr ◽  
Antimicrobial Activities ◽  
Spectroscopic Techniques ◽  
Good Activity ◽  
Gram Negative ◽  
E Coli ◽  
Chromatographic Techniques ◽  
Inhibition Zones ◽  
First Time

One new 3-prenyl-2-flavene, named baphiflavene A, 1, and eleven known compounds, 2-12, were isolated and reported for the first time from Baphia massaiensis using several chromatographic techniques. Their structures were elucidated using different spectroscopic techniques; 1D and 2D-NMR, HRMS, GC-MS, UV/Vis, FTIR and by comparison with literature data. The isolates were tested against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli and Candida albicans to establish their preliminary antimicrobial activities. The results revealed that compound 1 had moderate activities against both Gram positive ( B. subtilis and S. aureus) and Gram negative ( E. coli and P. aeruginosa) bacteria, and good activity against C. albicans with inhibition zones of 10–23 mm (compared to 19 mm for chloramphenicol and miconazole standards). To the best of our knowledge, this is the first phytochemical work reported on Baphia massaiensis.

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