Green synthesis, characterization and antimicrobial activities of Copper nanoparticles from the rhizomes extract of Picrorhiza kurroa

2021 ◽  
Vol 09 ◽  
Author(s):  
Vinit Prakash ◽  
Anjana Kumari ◽  
Harpreet Kaur ◽  
Manoj Kumar ◽  
Sumeet Gupta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Copper Nanoparticles ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Spectral Studies ◽  
Blue Color ◽  
Picrorhiza Kurroa ◽  
Fungal Strains ◽  
Gastrointestinal Problems ◽  
Hydroethanolic Extract

Background: Green synthesized nanoparticles from the solvent extract of various plant parts show better biological activities as compared to parent solvent plant extract. Traditionally rhizomes of Picrorhiza kurroa are used to cure various diseases like diarrhea, fever, jaundice, eye infection, skin problems, asthma, arthritis, cancer, diabetes, gastrointestinal problems. Objectives: Present study describes the synthesis of copper nanoparticles from a hydroethanolic extract of P. kurroa rhizomes (CuNPs-Pk) and their evaluation for antimicrobial activities against gram-negative, gram-positive bacterial, and fungal strains. Methods: The solution of copper sulfate and hydroethanolic extract of rhizomes of P. kurroa was mixed with help of a magnetic stirrer at 60°C temperature for 1 h. The blue color of CuSO4.5H2O changed to brownish-black colored copper nanoparticles within 10 minutes. These nanoparticles were centrifuged at 4000 rpm for 20 min, washed with ethanol, followed by deionized water, dried, and were characterized by Ultra violet–visible (UV-Vis) absorption spectra, Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (TEM). Different concentrations of hydroethanolic extract of Picrorhiza kurroa rhizomes (HEEPk), CuNPs-Pk and copper oxide nanoparticles (bare CuO) ranging from 100-400 ppm had been studied against selected bacterial and fungal strains by using the well plate diffusion method. Ciprofloxin and fluconazole were used as standard and Dimethyl sulfoxide (DMSO) as a control for selected strains. Results: The UV–Vis spectral studies confirmed the surface plasmon resonance of green-synthesized CuNPs-Pk. The particle size was found to be 275-285 nm. FTIR analysis of biosynthesis nanoparticles have been confirm the presence of various functional groups (flavonoids, glycosides, tannins, phenols). SEM and TEM of biosynthesized nanoparticles have predicted their spheric al shape and their size (20-40 nm) and These particles have shown effective antimicrobial activities against selected pathogenic organisms viz. Escherichia coli, Staphylococcus aureus, and Aspergillus niger than that of HEEPk and bare CuO. Conclusions: The CuNPs-Pk show effective antimicrobial activities against bacterial and fungal pathogens as compared to HEEPk and bare CuO.

scholarly journals Synthesis, Spectroscopic, and Antimicrobial Study of Binary and Ternary Ruthenium(III) Complexes of Ofloxacin Drug and Amino Acids as Secondary Ligands

Crystals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 225
Author(s):  
Ahmed M. Naglah ◽  
Mohamed A. Al-Omar ◽  
Abdulrahman A. Almehizia ◽  
Ahmad J. Obaidullah ◽  
Mashooq A. Bhat ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Amino Acids ◽  
Analysis Data ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
Element Analysis ◽  
X Ray ◽  
Paper Disc

This article aimed to synthesis, spectroscopic, physicochemical characterizations, and catalytic studies on some ofloxacin (OFL) complexes with ruthenium(III) [Ru(III)] metal ions. The Ru(III)-OFL complex, [Ru(OFL)2(Cl)2]Cl and two mixed-ligand complexes, [Ru(OFL)(AA)(H2O)2]Cl2 derived from OFL as the primary ligand and amino acids [AA; either glycine (Gly) or alanine (Ala)] as the secondary ligands, were synthesized and characterized using microanalytical, spectroscopic, and physical techniques including element composition, molar conductivity, infrared, electronic spectra, thermal, X-ray powder diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX), Scanning electron microscopy (SEM), and Transmittance electron microscopy (TEM) analyses. The element analysis data describe the formation of 1:2 [Ru(III):OFL] and 1:1:1 [Ru(III):OFL:AA] complexes. The octahedral geometry of the complexes was confirmed by their magnetic moment and diffused reflectance. Fourier Transform Infrared spectroscopic (FTIR) measurements suggested that the ligands chelated with Ru(III) ions through the nitrogen atoms of the piperazine ring. In vitro antibacterial efficiency of the OFL compounds was evaluated by paper disc diffusion method. Significant antimicrobial activities were observed for some compounds of the series.

scholarly journals Synthesis, Characterization and Biological Evaluation of Fe(III) and Cu(II) Complexes with 2,4-Dinitrophenyl hydrazine and Thiocyanate Ions

2019 ◽  
Vol 31 (4) ◽  
pp. 780-784
Author(s):  
P. Manimaran ◽  
S. Balasubramaniyan
Keyword(s):  
Antioxidant Activity ◽  
Metal Complexes ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Ligand ◽  
Antimicrobial Activities ◽  
Biological Evaluation ◽  
Diffusion Method ◽  
Spectral Studies

The metal complexes of Fe(III) and Cu(II) were prepared by using 2,4-dinitrophenyl hydrazine (DNPH) and thiocyanate (SCN) with stirrer refluxed for about 6 h. The prepared Fe(III) and Cu(II) complexes were characterized by elemental analysis, molar conductance, magnetic susceptibility and electronic spectrum, FT-IR spectral studies. The result suggested the octahedral geometry for Fe(III) and Cu(II) complexes. Powder X-ray diffraction indicate the crystalline nature of the metal complexes. The antimicrobial activities of the Fe(III) and Cu(II) complexes were tested with various micro organisms by disc diffusion method. The antimicrobial results indicate that the metal complexes are highly active with compared to the free ligand. The in vitro antioxidant activity of the free ligand and its metal complexes was assayed by radical scavenging activity (DPPH). The result proposed that Fe (III) and Cu(II) complexes exhibited strong antioxidant activity than that of the ligand.

scholarly journals FACILE SYNTHESIS, SPECTRAL STUDIES, DFT CALCULATIONS AND BIOLOGICAL ACTIVITIES OF NOVEL NI (II), CU (II), AND PD (II) COMPLEXES OF THIADIAZOLE ANALOGS

2017 ◽  
Vol 9 (4) ◽  
pp. 185
Author(s):  
Priyanka Bhatt ◽  
S. Deepthi ◽  
Ch. Ravi Shankar Kumar ◽  
Anjali Jha
Keyword(s):  
Conventional Method ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Spectral Studies ◽  
Spectroscopic Techniques ◽  
Anticancer Activities ◽  
Facile Synthesis ◽  
Spectral Techniques ◽  
Antibacterial And Antifungal Activities

Objective: A facile synthesis of some novel Schiff base derivatives of 2-substituted-5-amino-thiadiazoles along with their Ni (II), Cu (II), and Pd (II) complexes were achieved by sonication and the conventional method. In addition to establish the structure by DFT studies and to explore antimicrobial and anticancer activities of these novel compounds.Methods: The precursor 2-substituted-5-amino-thiadiazoles (T1-T3), target ligands and their metal complexes were synthesized by ultra-sonication and conventional means. The isolated products were thoroughly characterized by physical and spectroscopic techniques including 1H-NMR, [13]C-NMR and IR spectroscopy. All characterized compounds were screened for antimicrobial activities using well diffusion method, and MTT assay was performed for cytotoxicity.Results: All novel compounds were synthesized by a green route i.e. ultra sonication and a noticeable improvement in yield with shorter reaction time than the conventional method were observed. The octahedral geometry was proposed for Ni (II)/Cu (II) complexes whereas square planar for Pd (II) complexes on the basis of the spectral techniques which were supported by DFT analysis by Gaussian03. On the analysis of antimicrobial activities, the compound T7 and T10 showed maximum antibacterial and antifungal activities respectively. However, compounds T25, T37, T31 found to be a potential cytotoxic compound with IC50 value 0.469, 0.865 and 1.131 μM respectively.Conclusion: Analysis of synthetic protocol, it could be concluded that ultra-sonication is the better method to synthesize these potential biological active moiety. On the whole Cu (II) and Ni (II) complexes showed promising activity towards all microorganisms while Pd (II) complex emerged an excellent moiety in carcinoma cell line.

scholarly journals Synthesis, Characterization and Antimicrobial Activity of Mixed Schiff Base Ligand Complexes of Co(II), Ni(II), Cu(II) and Zn(II)

2011 ◽  
Vol 8 (3) ◽  
pp. 1258-1263 ◽  
Author(s):  
A. K. Mapari ◽  
M. S. Hate ◽  
K. V. Mangaonkar
Keyword(s):  
Antimicrobial Activity ◽  
Thermogravimetric Analysis ◽  
Schiff Bases ◽  
Free Ligand ◽  
Antimicrobial Activities ◽  
Mixed Ligand ◽  
Mixed Ligand Complexes ◽  
Azomethine Nitrogen ◽  
Diffusion Method ◽  
Spectral Studies

The mixed ligand complexes of Co(II), Ni(II), Cu(II) and Zn(II) with Schiff basesN-(2-hydroxy-1-naphthylidene)-4-methylaniline (L1H) andN-(2-hydroxybenzylidene)-2,3-dimethylaniline (L2H) have been synthesized and characterized. The resulting complexes were characterized by elemental analysis, thermogravimetric analysis, magnetic moment measurements, conductivity measurements,1H NMR, IR, UV-visible and ESR spectral studies. The Schiff bases acts as bidentate monobasic ligands, coordinating through deprotonated phenolic oxygen and azomethine nitrogen atoms. The complexes are non-electrolytic in DMSO. The presence of the two coordinated water molecules in these complexes was indicated by IR spectra and thermogravimetric analysis of the complexes. From the analytical and spectral data the stoichiometry of these complexes have been found to be [M(L1)(L2)(H2O)2] {where M = Co(II) , Ni(II), Cu(II) and Zn(II)}. It is found that Co(II), Ni(II), Cu(II) and Zn(II) complexes exhibited octahedral geometry. The antimicrobial activities of ligands and their mixed ligand complexes were screened by disc diffusion method. It is found that the metal complexes have higher antimicrobial activity than the free ligand.

scholarly journals Phytochemical Activities of Artemissia absinthium and Butea monosperma Plant Extracts

2021 ◽  
pp. 1-7
Author(s):  
Fatima Waheed ◽  
Shaista Yaqoob ◽  
Qurban Ali ◽  
Arif Malik
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Plant Extracts ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Antimicrobial Drugs ◽  
Butea Monosperma ◽  
Fungal Strains ◽  
Inhibition Zones ◽  
Agar Well Diffusion Method

With the developing time, there is a tremendous need to deliver highly potential antimicrobial drugs and treatment from different herbaceous plants because plants are now days commonly used to provide resistance against disease-causing pathogens. This study has been done by using agar well diffusion method. The bacterial along with fungal strains were collected and were cultured on agar plates. Afterwards, these plates were put in incubate for 24 hours at 370C temperature. Within this duration, the zones were developing all around the plates, wherever plant extracts were poured. The activities of microorganisms were measured by using inhibition diameter zones. The inhibition zones were shown clearly on petri plates. Antimicrobial activities of plant extracts of Artemissia absinthium and Butea monosperma tested against bacterial and fungal strains viz. Pseudomonas aeruginosa, Bacillus subtilis and Escherichia coli as well as fungal strains were also used viz. Aspergillus niger and Fusarium oxysporum.

scholarly journals BIOSYNTHESIS OF COPPER NANOPARTICLES USING PARTHENIUM HYSTEROPHORUS LEAF EXTRACT AND SCREENING ITS ANTIMICROBIAL ACTIVITY

2019 ◽  
pp. 93-99
Author(s):  
ROHINI SAHAYA MARY ◽  
MAHESH MK ◽  
PRUTHVI ML
Keyword(s):  
Copper Nanoparticles ◽  
Leaf Extract ◽  
Color Change ◽  
Morphological Characteristics ◽  
Antimicrobial Activities ◽  
Parthenium Hysterophorus ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
Resonance Band ◽  
High Inhibition

Objective: The aim of this study is to synthesis the copper nanoparticles (CuNPs) using the leaf extract of Parthenium hysterophorus. Methods: Dry and fresh leaf extract was prepared and CuSo4 was added. The color change was noted and recorded by ultraviolet–visible spectrophotometer. The morphological characteristics were analyzed by scanning electron microscopy (SEM). Antimicrobial activities were performed by the disc diffusion method. Results: The color change indicates the production of CuNPs. Surface plasmon resonance band was observed around 599 nm and 572 nm for fresh and dry samples of P. hysterophorus leaf extract. SEM confirms the formation and the crystalline nature of CuNPs and X-ray diffraction studies show the particle size. The antibacterial potentials of the CuNPs were studied and have shown good high inhibition activity against Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, and Pseudomonas aeruginosa at different concentrations in compare to fungi species. Conclusion: This method is effective and environmental friendly for the synthesis of CuNPs using leaf extract of Parthenium hysterophorus.

scholarly journals Green Synthesis, Characterization and Antimicrobial activity of Silver Nanoparticles from the Extract of Lagerstroemia speciosa

2021 ◽  
Vol 37 (3) ◽  
pp. 648-655
Author(s):  
Vinit Prakash ◽  
Harpreet Kaur ◽  
Anjana Kumari ◽  
Manoj Kumar ◽  
Sumeet Gupta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Kidney Diseases ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Ag Nps

Lagerstroemia speciosais commonly known as Banaba or Jarul which is used to get rid of various ailments such as fever, urinary infection, decongestion, diarrhoea, mouth ulcers, astringent, diabetes mellitus, kidney diseases, abdominal pains etc. The present work, describes the green synthesis of silver nanoparticles from ethanolic extract of fruits of L.speciosa (Ls-Ag NPs) and their analysis for antimicrobial activities. The characterisation of so obtained nanoparticles have been carried out with help of Field emission scanning electron microscopy (FESEM) and High-resolution transmission electron microscopy (HRTEM). Further, antimicrobial activities of ethanolic extract of fruits of L.speciosa(Ee-Ls), silver oxide (Ag2O), and Ls-Ag NPs have been examined by using well-diffusion method against two bacterial strains: Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) and one fungal strain: Aspergillus niger.It has been observed that the biosynthesized Ls-Ag NPs possess much effective antimicrobial activity against selected strains as compared to Ee-Ls and Ag2O.

scholarly journals Nickel Oxide-Catalyzed Synthesis of 4-Amino-2H-Chromenes: Its Application in Antimicrobial Studies and Towards Protein Docking

2019 ◽  
Vol 31 (5) ◽  
pp. 1049-1056
Author(s):  
A. Neela ◽  
T. Clarina ◽  
V. Rama
Keyword(s):  
Electron Microscopy ◽  
Nickel Oxide ◽  
Aromatic Aldehydes ◽  
Catalytic Amount ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
Protein Docking ◽  
Diffusion Method ◽  
Nickel Oxide Nanoparticles ◽  
Antimicrobial Studies

This work involves the synthesis of densely functionalized 2-amino-4H-chromenes by domino Knoevenagel-Michael-cyclization reaction of aromatic aldehydes, β-naphthol and malononitrile in the presence of a catalytic amount of a heterogeneous and reusable green NiO nanoparticle at 50 °C. The biogenic nickel oxide nanoparticles are characterized by Fourier transform infrared radiation, X-ray diffraction analysis, scanning electron microscopy and transmission electron microscopy. The synthesized chromenes are characterized by IR, NMR spectra. The synthesized chromene derivatives are studied for microbial inhibition by Kirby-Bauer disc diffusion method using amikacin and flucanazole as positive control. The compounds are found to have good to moderate antimicrobial activities. Their bio evaluation has been carried out with a protein and identified promising ligands for Mycobacterium tuberculosis InhA through molecular docking.

scholarly journals Evaluation of Phenolic Compounds and Antioxidant and Antimicrobial Activities of Some Common Herbs

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Muhammad Abdul Qadir ◽  
Syeda Kiran Shahzadi ◽  
Asad Bashir ◽  
Adil Munir ◽  
Shabnam Shahzad
Keyword(s):  
Antimicrobial Activity ◽  
Linoleic Acid ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Antioxidant And Antimicrobial Activities

The study was designed to evaluate the phenolic, flavonoid contents and antioxidant and antimicrobial activities of onion (Allium cepa), garlic (Allium sativum), mint (Mentha spicata), thyme (Thymus vulgaris), oak (Quercus), aloe vera (Aloe barbadensis Miller), and ginger (Zingiber officinale). All extracts showed a wide range of total phenolic contents, that is, 4.96 to 98.37 mg/100 g gallic acid equivalents, and total flavonoid contents, that is, 0.41 to 17.64 mg/100 g catechin equivalents. Antioxidant activity (AA) was determined by measuring reducing power, inhibition of peroxidation using linoleic acid system, and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity. Different extracts inhibited oxidation of linoleic acid by 16.6–84.2% while DPPH radical scavenging activity (IC50 values) ranged from 17.8% to 79.1 μg/mL. Reducing power at 10 mg/mL extract concentration ranged from 0.11 to 0.84 nm. Furthermore the extracts of these medicinal herbs in 80% methanol, 80% ethanol, 80% acetone, and 100% water were screened for antimicrobial activity by disc diffusion method against selected bacterial strains, Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pasteurella multocida, and fungal strains, Aspergillus niger, Aspergillus flavus, Rhizopus solani, and Alternaria alternata. The extracts show better antimicrobial activity against bacterial strains as compared to fungal strains. Results of various assays were analyzed statistically by applying appropriate statistical methods.

scholarly journals Solid acidic FeCl3/Bentonite catalyzed solvent-free condensation: Synthesis, spectral studies and antimicrobial activities of some aryl hydrazine Schiff’s bases

2017 ◽  
Vol 71 (2) ◽  
pp. 127
Author(s):  
Vadamalai Ananthi ◽  
Kaliyaraj Rajalakshmi ◽  
Inbasekaran Muthuvel ◽  
Ganesamoorthy Thirunarayanan
Keyword(s):  
Microwave Irradiation ◽  
Spectroscopic Data ◽  
Antimicrobial Activities ◽  
Solvent Free ◽  
Diffusion Method ◽  
Spectral Studies ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Phenyl Hydrazine ◽  
Micro Analysis

<p>Some aryl hydrazide derivatives have been synthesized including 1-(3-chloro-4-nitrophenyl)-2-(3-substituted benzylidene) hydrazines by  FeCl<sub>3</sub>/Bentonite catalyzed solvent-free  condensation of  substituted phenyl hydrazine and aldehydes under microwave irradiation.  The yields of the hydrazides are more than 70%.  The synthesized hydrazides are characterized by the physical constants, micro analysis and spectroscopic data.  Effect of catalyst,  solvent effect  substituent effect and optimization of the catalyst was studied by the percentage of isolated yields.  From the catalyst optimization, the present study catalyst gave the better yield of products.  The antimicrobial activities of all synthesized of 1-(3-chloro-4-nitrophenyl)-2-(3-substituted benzylidene) hydrazines have been evaluated using Bauer-Kirby disc diffusion method.</p>

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