scholarly journals The Antibacterial Efficacy of Annona muricata (Linn) Leaves against Some Enteric Bacteria

2020 ◽  
pp. 19-29
Author(s):  
Kehinde Yemisi Akinde ◽  
Ebenezer Oluyemi Dada
Keyword(s):  
Antibacterial Activity ◽  
Aqueous Ethanol ◽  
Enteric Bacteria ◽  
Dilution Method ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Leaf Extracts ◽  
Methanol Extracts ◽  
Test Organisms

Aims: This research was carried out to evaluate the susceptibility patterns of some enteric bacteria to aqueous, ethanol and methanol extracts of Annona muricata leaf. Study Design: Experimental design. Place and Duration of Study: Department of Microbiology, Federal University of Technology, Akure, Ondo State, Nigeria. Between January, 2019 and May, 2019. Methodology: Extraction of bioactive components of leaves was done by maceration and phytochemical screening was carried out on the leaf extracts to determine the bioactive components present. The bacteria isolates were subjected to antibiotic sensitivity test using standard methods while the  antibacterial  activity  of  the  plant  extracts on human enteric bacteria was  determined  using  agar well dilution method. A. muricata bark extracts were purified using column chromatography method. The minimum inhibitory and minimum bactericidal concentrations (MIC/MBC) of the extracts was performed using tube dilution technique. Results: The phytochemical screening of leaf extracts showed presence of saponins, tannins, flavonoids, terpenoid and glycosides. Antibacterial activities of the leaf extracts revealed that aqueous, ethanol and methanol extracts had highest zone of inhibition of 22.00±0.58 mm, 24.67±0.33 mm and 24.00±0.58 mm respectively on Salmonella typhi. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) for ethanol extract ranged between 25-100 mg/ml and 50-200 mg/ml respectively while methanol extract ranged between 25-50mg/ml and 50-100 mg/ml respectively. Ciprofloxacin (CPX) was significantly (P≤0.5) the most active of all the antibiotics used against the test organisms (both clinical and typed isolates). Conclusion: This research revealed that A. muricata leaf extracts possesses antibacterial activity against human enteric bacteria isolates used in this study. The commercial antibiotics were observed to be effective in inhibiting the test organisms. The purified extracts of A. muricata leaf showed higher inhibitory effect compared to crude extracts which can serve as ingredient for the production of novel antibacterial agents for the treatment of infections caused by enteric bacteria.

scholarly journals Susceptibility Patterns of Some Enteric Bacteria to Crude and Purified Bark Extracts of Annona muricata L.

2020 ◽  
pp. 21-33
Author(s):  
Ebenezer Oluyemi Dada ◽  
Kehinde Yemisi Akinde
Keyword(s):  
Antibacterial Activity ◽  
Antibiotic Sensitivity ◽  
Enteric Bacteria ◽  
Antibacterial Activities ◽  
Bark Extract ◽  
Dilution Method ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Chromatography Method

Aims: This research was carried out to evaluate the susceptibility pattern of some enteric bacteria to crude and purified extracts of Annona muricata bark. Study Design: Experimental design. Place and Duration of Study: Department of Microbiology, Federal University of Technology, Akure, Ondo State, Nigeria. Between January, 2019 and May, 2019. Methodology: Extraction of bioactive components of bark was done by maceration and phytochemical screening was carried out on the bark extracts to determine the bioactive components present. The bacteria isolates were subjected to antibiotic sensitivity test using standard methods while the antibacterial activity of the plant extracts on human enteric bacteria was determined using agar well dilution method. A. muricata bark extracts were purified using column chromatography method. The minimum inhibitory and minimum bactericidal concentrations (MIC/MBC) of the extracts were performed using tube dilution technique. Results: The quantitative phytochemical screening for bark extract revealed that glycosides (7.06±0.04, 34.67±0.02 and 19.35±0.01) extracted with aqueous, ethanol and methanol respectively is the most abundant phytochemical constituents. The antibacterial activities of the bark extracts revealed that aqueous showed no inhibition to none while ethanol and methanol inhibited all the test organisms. The highest value of minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) for both ethanol and methanol bark extracts was 50 mg/ml and 100 mg/ml respectively. Conclusion: This research revealed that A. muricata bark extracts possesses antibacterial activity against human enteric bacteria isolates used in this study. The purified extracts of A. muricata bark showed higher zones of inhibition which indicates that it can compete well with standard antibiotics and it may also serve as a substitute to the commercially available antibiotics that can be used for the treatment of infections caused by enteric bacteria.

scholarly journals In-Vitro Antibacterial Properties of Ethanol and Methanol Extracts of Betel Leaves Collected from Different Areas of Bangladesh

2019 ◽  
Vol 34 (2) ◽  
pp. 125-127
Author(s):  
Md Sahadat Hossain ◽  
Kamal Kanta Das ◽  
Mrityunjoy Acharjee
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Antimicrobial Activities ◽  
Human Pathogens ◽  
Leaf Extracts ◽  
Methanol Extracts ◽  
Betel Leaf ◽  
Test Organisms ◽  
Ethanol Extracts

Current investigation depicted in-vitro antibacterial activity of available betel leaf (sweet, hot and fresh betel leaves) collected from different place of Bangladesh. The in-vitro antimicrobial activities of the ethanol and methanol extracts of the betel leaf were noticeable against tested bacteria. Among 3 categories of samples, the antibacterial action of ethanol extracts of hot betal leaf was found the highest against most of the bacteria except Klebsiella sp. and Staphylococcus sp., while the methanol extract showed antibacterial activity against all the pathogenic strains tested. The highest MIC (1mg/ml) and the lowest MIC values (25mg/ml) of hot betel leaf and sweet betel leaf were found against E. coli and all test organisms, respectively. This evidence revealed that betel leaf extracts can be used to combat against human pathogens. Bangladesh J Microbiol, Volume 34 Number 2 December 2017, pp 125-127

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals Evaluation of the Larvicidal Potential of the Leaf Extracts of Hyptis suaveolens Poit against Anopheles Mosquitoes

2018 ◽  
pp. 1-9
Author(s):  
Amaka, John I. ◽  
Attah, D. Daniel ◽  
Obisike, Victor U. ◽  
Benedict, Aboje G.
Keyword(s):  
Mosquito Control ◽  
Larval Instar ◽  
Test Organism ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Anopheles Mosquitoes ◽  
Lc50 Value ◽  
Hyptis Suaveolens ◽  
Test Organisms ◽  
Layer Chromatography

This study evaluated the larvicidal potential of the ethanolic and aqueous leaf extracts of Hyptis suaveolens Poit on the 4th larval instar of laboratory-reared Anopheles spp at varying concentrations of 0.1ml, 0.2ml, 0.3ml, 0.4ml and 0.5ml for specified periods of 24hrs, 48hrs and 72hrs. Qualitative phytochemical screening of the leaf extracts identified bioactive components like alkaloid, saponin, phenol, anthraquinone and flavonoid. The LC50 and LC90 values obtained indicate that the ethanolic leaf extracts of Hyptis suaveolens Poit had the greatest toxicity on the test organisms within 24hrs of exposure at median LC50 value of 0.485ml compared to the LC50 value of 0.625ml by its aqueous extract. The relative median potency estimates indicate that within 24 hrs, the ethanolic Hyptis suaveolens Poit was 0.161 times more potent on the test organism than aqueous Hyptis suaveolens Poit. The result of this research, therefore, underscores the efficacy of Hyptis suaveolens Poit as an eco-friendly alternative in Anopheles mosquito control. It is, therefore, recommended that quantitative phytochemical screening, application of column chromatography as well as thin layer chromatography be carried out on the extracts to isolate and purify toxic phytochemicals with larvicidal potentiality.

scholarly journals Antibacterial Activityof Aqueous And Ethanolic Leaf Extracts Of Vernonia Amygdalina On Selected Species Of Gram Positive And Gram Negative Bacteria

2013 ◽  
Vol 2 (1) ◽  
pp. 147-152 ◽  
Author(s):  
AM Bukar ◽  
MA Isa ◽  
HS Bello ◽  
AS Abdullahi
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Drug ◽  
Gram Negative Bacteria ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Pseudomonas Species

The phytochemical screening and antibacterial activity of ethanolic and Methanolic leaves extract of Vernonia amygdalina against five clinical isolates (Staphylococcus aureus, E. coli, Pseudomonas species, Salmonella species and Proteus species) was determined using standard method of analysis. The results of the antibacterial activity of ethanol, methanol and aqueous extract of leaves of V. amygdalina have diameters ranging between 0.4 to 10mm. The plant extracts from the plants had profound activities against gram-positive than gram negative bacteria. From the above studies, it has clearly indicated that V. amygdalina extract may represent new sources of antibacterial drug, if the phytoactive components are purified and proper dosage are determined for administration. International Journal of Environment, Volume-2, Issue-1, Sep-Nov 2013, Pages 147-152 DOI: http://dx.doi.org/10.3126/ije.v2i1.9217

scholarly journals Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yidnekachew Girma ◽  
Tamene Milkessa Jiru
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Pathogenic Fungi ◽  
Considerable Effect ◽  
Dilution Method ◽  
Leaf Extracts ◽  
Superficial Infection ◽  
Methanol Extracts ◽  
Conyza Bonariensis

Plants have been used since long time ago to treat infectious diseases and are considered as important sources of new antimicrobial agents. In this study, crude extracts from leaves of Conyza bonariensis were prepared using methanol, ethyl acetate, hexane, and chloroform. Antimicrobial activity of the extracts was evaluated against pathogenic fungi causing superficial infection (Candida albicans, Malassezia globosa, and Malassezia furfur). Results demonstrated that all extracts had different effects against all the tested fungi with the exception of crude extract using hexane which did not show any effect against M. furfur. A strong effect was observed with chloroform and hexane extracts on C. albicans (32.60 ± 4.69 mm and 27.00 ± 1.00 mm), respectively. While, ethyl acetate and methanol extracts showed the best effect against M. furfur (30.80 ± 1.71 mm and 27.00 ± 1.00 mm), respectively. Moreover, the ethyl acetate showed a considerable effect on M. globosa (25.03 ± 1.05 mm). Minimum inhibitory concentration (MIC) of the fractions was also determined by the microbroth dilution method. The results recorded as the MIC values of the tested extracts against fungi varied from 0.19 ± 0.00 to 66.66 ± 2.86 mg/mL. Ethyl acetate was the best and powerful extract with the lowest MIC value of 0.190.19 ± 0.00 mg/mL for all tested fungi followed by chloroform and methanol extracts with the MIC values ranging from 0.19 ± 0.00 to 0.78 ± 0.00 mg/mL and 0.84 ± 0.68 to 1.56 mg/mL, respectively. Concerning minimum bactericidal concentration (MFC), ethyl acetate was the most potent extract with a MFC value of 0.190.19 ± 0.00 mg/mL for C. albicans and M. furfur. Higher (0.39 mg/mL) MFC was recorded against M. globosa by this extract. In conclusion, solvent extracts of some C. bonariensis can be used to treat infections with pathogenic fungi such as C. albicans, M. furfur, and M. globosa. Further studies should consider this plant as one of the best candidates for the discovery of potent antimicrobial compounds that treat superficial infections.

scholarly journals PHYTOCHEMICAL, GAS CHROMATOGRAPHY WITH MASS SPECTROMETRY ANALYSIS OF ANDROGRAPHIS SERPYLLIFOLIA METHANOL LEAF EXTRACT AND ITS ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES

2019 ◽  
pp. 343-347
Author(s):  
MADHANKUMAR R ◽  
MURUGESAN S
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Mass Spectrometry Analysis ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Agar Disc ◽  
Soxhlet Apparatus

Objective: The present study is to evaluate the preliminary study of phytochemical screening and biological applications of Andrographis serpyllifolia methanol leaf extracts. Methods: The methanol leaf extracts of A. serpyllifolia was prepared using Soxhlet apparatus and the extract was analyzed using gas chromatography with mass spectrometry (GC-MS). In vitro antioxidant activity was determined by superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase. Further, the antibacterial activity of methanolic leaf extract of A. serpyllifolia was tested against various human pathogens by using agar disc diffusion method. Results: Preliminary phytochemical screening and GC-MS results revealed phenols, aromatic carboxylic acids, and esters in the chloroform extract to be the molecules responsible for the antioxidant and antibacterial activity of A. serpyllifolia methanol extract and fractions showed the presence of various secondary metabolites present. Conclusion: The present study strongly recommended that the methanolic extract of A. serpyllifolia leaves possesses compounds that inhibit the growth of microbes as wells excellent antioxidant activities. The study further suggested the potential therapeutic use of these extract in cancer study.

scholarly journals The Antibacterial Activity of Aqueous and Ethanolic Leaf Extracts of Balanites aegyptiaca (l.) Del Plant on Some Selected Clinical Human Pathogens

2020 ◽  
pp. 51-66
Author(s):  
G. C. Ezemokwe ◽  
J. C. Aguiyi ◽  
F. P. Chollom
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Activity Index ◽  
Phytochemical Screening ◽  
Human Pathogens ◽  
Leaf Extracts ◽  
Balanites Aegyptiaca ◽  
Minimal Inhibitory Concentrations ◽  
Significant Difference ◽  
Zones Of Inhibition

The antibacterial activity of the leaf extract of Balanites aegyptiaca plant was investigated on five selected clinical common human pathogens: Staphylococcus aureus, Streptococcus pneumonia, Salmonella typhimurium, Shigella dysentriae and Escherichia coli, in vitro. The phytochemical screening, susceptibility testing and Minimal Inhibitory Concentrations were determined. Preliminary phytochemical screening of the leaf extracts indicated the presence of alkaloids, cardiac glycosides, flavonoids, glycosides, resins, saponins, steroids, tannins, and carbohydrates in varying concentrations. Ethanolic leaf extract was more effective. The extracts inhibited the growth of isolates with increasing concentrations, ranging from 8-12 mm zones of inhibitions, showing the susceptibility of the test organisms. S. dysentriae had the highest susceptibility for both extracts with 12 mm and 13 mm zones of inhibition for aqueous and ethanolic extracts respectively at the highest concentration of 400 mg/ml. S. aureus showed the least susceptibility with 8 mm for aqueous extract while S. typhimurium showed the least susceptibility for the ethanolic extract with 9mm zones of inhibition. The analysis of variance results on zones of inhibition revealed a significant difference for both extract treatments, concentrations, and interactions between the treatments and concentrations. The Minimal Inhibitory Concentrations determined from the corresponding concentration-response curves showed that S. dysentriae had the least value of 79.433 mg/ml while the aqueous extracts against S. aureus and S. typhimurium were the largest with 125.893 mg/ml value. The activity index and activity were also deduced to measure activity. The antimicrobial activity of leaf extracts of B. aegyptiaca on bacteria of the tested isolates have been established in this study and justify the claims by the traditional healers in its use to treat infectious diseases.

scholarly journals Antibacterial activities and phytochemical screening of crude extract of Carica papaya leaf against selected pathogens

2020 ◽  
Vol 26 (2) ◽  
pp. 165-170
Author(s):  
A.E. Ajiboye ◽  
R.A. Olawoyin
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Aqueous Extract ◽  
Carica Papaya ◽  
Antibacterial Activities ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Crude Extracts ◽  
Test Organisms

Carica papaya commonly known as paw paw belongs to the family of Curcubitaceae and commonly grown in tropical regions. It possesses  antimicrobial, antihelmintic and antioxidant properties. The study assessed the antibacterial potency of Carica papaya against Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus. Acetone and aqueous extracts of the leaves of Carica papaya were obtained using standard methods. The antibacterial activity of the extracts was done using agar well diffusion methods. The Minimum inhibitory and Minimum bactericidal concentrations were done using standard procedures. The antibacterial activities of the crude extracts of Carica papaya against the test organisms revealed that acetone extract showed maximum zone of inhibition on Staphylococcus aureus with a diameter of 17.90±0.10mm at 500 mg/ml and the lowest inhibitory effect on Klebsiella pneumoniae with a zone of 6.50±0.50mm at 100 mg/ml, the aqueous extract showed maximum zone of  inhibition on Staphylococcus aureus with a diameter of 15.50±0.50mm at 500 mg/ml and the lowest zone of inhibition was on Staphylococcus aureus with a diameter of 6.50±0.50mm at 100 g/ml. The Minimum Inhibitory Concentration of acetone and aqueous extract was 40 mg/ml and 50mg/ml against Klebsiella pneumoniae respectively. The Minimum Bactericidal Concentration of the extracts ranges from 40-60mg/ml. The qualitative  phytochemical screening result revealed the presence of tannins, saponnin, alkaloids and steroid. The quantitative phytochemicals revealed 0.70%  of flavonoids, 0.48% of alkaloids, 1.02% of tannin, 0.11% of steroids and 1.08% of glycoside. The result obtained revealed that crude extracts of Carica papaya leaves has antibacterial activities against the test organisms. Keywords: Carica papaya, Phytochemical screening, Pathogens, Antibacterial  activity

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