scholarly journals In situ rumen degradation characteristics and bacterial colonization of whole cottonseed, cottonseed hull and cottonseed meal with different gossypol content

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wei-Kang Wang ◽  
Yan-Lu Wang ◽  
Wen-Juan Li ◽  
Qi-Chao Wu ◽  
Kai-Lun Yang ◽  
...  
Keyword(s):  
Cottonseed Meal ◽  
Free Gossypol ◽  
Degradation Mechanism ◽  
Bacterial Colonization ◽  
Nutrient Use Efficiency ◽  
Neutral Detergent Fibre ◽  
Cottonseed Hull ◽  
Whole Cottonseed ◽  
By Products

AbstractRegarding whole cottonseed (WCS), cottonseed meal (CSM), and cottonseed hull (CSH), in situ rumen incubation was applied to determine their nutrient and gossypol degradation characteristics and bacterial colonization profile in lactating Holstein cows. Nylon bags containing the cotton by-products were incubated for 0, 6, 12, 24, 36, 48 and 72 h in the rumen, respectively. The relationship between nutrient degradability and free gossypol (FG) content were examined, and the differences in the composition and inferred gene function of the colonized microbiota were studied. As a result, CSM presented highest effective degradability of dry matter, neutral detergent fibre and acid detergent fibre, but the highest effective degradability of crude protein was found in WCS. Free gossypol disappearance rate increased significantly in the first 6 h, and it reached approximately 94% at 72 h of incubation among all samples. The level of FG did not affect nutrient degradability of cotton by-products. Significant differences were noted in attached bacterial community structure among cotton by-products after 24 h rumen incubation. Among the most abundant taxa at genus level, a greater abundance of Cercis gigantea and Succiniclasticum was observed in WCS samples, whereas the CSH and CSM samples contained a greater proportion of Prevotella 1 and Rikenellaceae RC9 gut group. The redundancy analysis revealed that the level of neutral detergent fibre, ether extract, and FG in cotton by-products were significantly positive related with the composition of the attached bacteria. Collectively, our results revealed the dynamics of degradation characteristics, and the difference in the composition of bacterial colonization. These findings are of importance for the targeted improvement of cotton by-products nutrient use efficiency in ruminants and further understanding of the gossypol degradation mechanism in the rumen.

scholarly journals A cookbook for DNase Hi-C

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Maria Gridina ◽  
Evgeniy Mozheiko ◽  
Emil Valeev ◽  
Ludmila P. Nazarenko ◽  
Maria E. Lopatkina ◽  
...  
Keyword(s):  
Rational Design ◽  
Restriction Enzymes ◽  
Nucleotide Exchange ◽  
Computational Tools ◽  
3 Dimensional ◽  
Cultured Human Cells ◽  
Dna Ends ◽  
By Products ◽  
Robust Protocol

Abstract Background The Hi-C technique is widely employed to study the 3-dimensional chromatin architecture and to assemble genomes. The conventional in situ Hi-C protocol employs restriction enzymes to digest chromatin, which results in nonuniform genomic coverage. Using sequence-agnostic restriction enzymes, such as DNAse I, could help to overcome this limitation. Results In this study, we compare different DNAse Hi-C protocols and identify the critical steps that significantly affect the efficiency of the protocol. In particular, we show that the SDS quenching strategy strongly affects subsequent chromatin digestion. The presence of biotinylated oligonucleotide adapters may lead to ligase reaction by-products, which can be avoided by rational design of the adapter sequences. Moreover, the use of nucleotide-exchange enzymes for biotin fill-in enables simultaneous labelling and repair of DNA ends, similar to the conventional Hi-C protocol. These improvements simplify the protocol, making it less expensive and time-consuming. Conclusions We propose a new robust protocol for the preparation of DNAse Hi-C libraries from cultured human cells and blood samples supplemented with experimental controls and computational tools for the evaluation of library quality.

Evaluation of ozone technology as an alternative for degradation of free gossypol in cottonseed meal: a prospective study

2021 ◽  
pp. 1-11
Author(s):  
Alessandra de Cássia Romero ◽  
Maria Antonia Calori-Domingues ◽  
Adibe Luiz Abdalla ◽  
Pedro Esteves Duarte Augusto
Keyword(s):  
Prospective Study ◽  
Cottonseed Meal ◽  
Free Gossypol ◽  
A Prospective Study

scholarly journals Degradation Mechanism of Porous Metal-Organic Frameworks by In Situ Atomic Force Microscopy

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 722
Author(s):  
Ioanna Christodoulou ◽  
Tom Bourguignon ◽  
Xue Li ◽  
Gilles Patriarche ◽  
Christian Serre ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Degradation Mechanism ◽  
Metal Organic Frameworks ◽  
Porous Metal ◽  
Force Microscopy ◽  
Atomic Force ◽  
Metal Organic ◽  
The Media ◽  
Ph Conditions

In recent years, Metal-Organic Frameworks (MOFs) have attracted a growing interest for biomedical applications. The design of MOFs should take into consideration the subtle balance between stability and biodegradability. However, only few studies have focused on the MOFs’ stability in physiological media and their degradation mechanism. Here, we investigate the degradation of mesoporous iron (III) carboxylate MOFs, which are among the most employed MOFs for drug delivery, by a set of complementary methods. In situ AFM allowed monitoring with nanoscale resolution the morphological, dimensional, and mechanical properties of a series of MOFs in phosphate buffer saline and in real time. Depending on the synthetic route, the external surface presented either well-defined crystalline planes or initial defects, which influenced the degradation mechanism of the particles. Moreover, MOF stability was investigated under different pH conditions, from acidic to neutral. Interestingly, despite pronounced erosion, especially at neutral pH, the dimensions of the crystals were unchanged. It was revealed that the external surfaces of MOF crystals rapidly respond to in situ changes of the composition of the media they are in contact with. These observations are of a crucial importance for the design of nanosized MOFs for drug delivery applications.

Dietary Selection against Cottonseed Meal by Captive Northern Bobwhites

2018 ◽  
Vol 11 (3) ◽  
pp. 191-195 ◽  
Author(s):  
Amy L. Farthing ◽  
Thomas W. Schwertner ◽  
Heather A. Mathewson ◽  
Kimberly A. Guay
Keyword(s):  
Cottonseed Meal ◽  
Cultural Value ◽  
Feed Consumption ◽  
Published Data ◽  
Northern Bobwhites ◽  
Dietary Preference ◽  
Game Bird ◽  
Selection For ◽  
Whole Cottonseed ◽  
Preference Study

Gossypol, a secondary plant compound found in cotton (Gossypium spp.), is known to be toxic to a variety of animals, particularly monogastric mammals and birds. Because ruminants are resistant to gossypol, whole cottonseed and cottonseed meal have been used as a feed supplement for many decades. Concerns over gossypol toxicity arise because of its presence in cottonseed products, particularly livestock and wildlife feed. The concentration of cottonseed in the environment near livestock and wildlife feeding stations presents the possibility that it may be ingested in significant amounts by non-target wild animals, resulting in inadvertent gossypol dosing. A species of significant economic and cultural value is the Northern Bobwhite (Colinus virginianus). However, there are no published data regarding if Northern Bobwhites would consume cottonseed meal given the opportunity. We assessed selection for or against cottonseed meal by performing a dietary preference study. Birds (n =120) were given the choice between commercial game bird feed, scratch grains, and cottonseed meal containing 0.74% total gossypol. We measured feed consumption for five days. The birds’ diet consisted of 51.74% game bird feed, 37.72% scratch grains, and 10.54% cottonseed meal, and percentages varied significantly among all feed types (P≤0.001, n =360). We concluded that Northern Bobwhites selected against the cottonseed meal in favour of the game bird feed first and the scratch grains second. Our results suggest that given the choice, Northern Bobwhites will select against cottonseed meal if other feed choices are available.

Prediction of crude protein and neutral detergent fibre concentration in residues of in situ ruminal degradation of pasture samples by near-infrared spectroscopy (NIRS)

2016 ◽  
Vol 56 (9) ◽  
pp. 1504 ◽  
Author(s):  
J. P. Keim ◽  
H. Charles ◽  
D. Alomar
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Crude Protein ◽  
Near Infrared ◽  
External Validation ◽  
Neutral Detergent Fibre ◽  
Insufficient Amount ◽  
Ruminal Degradation ◽  
Animal Feedstuffs

An important constraint of in situ degradability studies is the need to analyse a high number of samples and often with insufficient amount of residue, especially after the longer incubations of high-quality forages, that impede the study of more than one nutritional component. Near-infrared spectroscopy (NIRS) has been established as a reliable method for predicting composition of many entities, including forages and other animal feedstuffs. The objective of this work was to evaluate the potential of NIRS for predicting the crude protein (CP) and neutral detergent fibre (NDF) concentration in rumen incubation residues of permanent and sown temperate pastures in a vegetative stage. In situ residues (n = 236) from four swards were scanned for their visible-NIR spectra and analysed for CP and NDF. Selected equations developed by partial least-squares multivariate regression presented high coefficients of determination (CP = 0.99, NDF = 0.95) and low standard errors (CP = 4.17 g/kg, NDF = 7.91 g/kg) in cross-validation. These errors compare favourably to the average concentrations of CP and NDF (146.5 and 711.2 g/kg, respectively) and represent a low fraction of their standard deviation (CP = 38.2 g/kg, NDF = 34.4 g/kg). An external validation was not as successful, with R2 of 0.83 and 0.82 and a standard error of prediction of 14.8 and 15.2 g/kg, for CP and NDF, respectively. It is concluded that NIRS has the potential to predict CP and NDF of in situ incubation residues of leafy pastures typical of humid temperate zones, but more robust calibrations should be developed.

Surface modification of sewage sludge derived carbonaceous catalyst for m-cresol catalytic wet peroxide oxidation and degradation mechanism

RSC Advances ◽  
2015 ◽  
Vol 5 (52) ◽  
pp. 41867-41876 ◽  
Author(s):  
Yang Yu ◽  
Huangzhao Wei ◽  
Li Yu ◽  
Tong Zhang ◽  
Sen Wang ◽  
...  
Keyword(s):  
Surface Modification ◽  
Sewage Sludge ◽  
Organic Synthesis ◽  
Degradation Mechanism ◽  
Peroxide Oxidation ◽  
Wet Peroxide Oxidation ◽  
Carbonaceous Catalyst ◽  
Catalytic Wet Peroxide Oxidation

Organic synthesis is used to investigate the degradation of m-cresol and the intermediates are identified by in situ NMR.

317 Effect of the Whole Cottonseed with Pre-treatment on the Digestibility, Productivity, and Milk Fat Profile of Dairy Cattle

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 172-173
Author(s):  
Xiaoge Sun ◽  
Yitong Su ◽  
YangYi Hao ◽  
Gaokun Liu ◽  
Yue Gong ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Milk Fat ◽  
Alkali Treatment ◽  
Mixed Alkali ◽  
Group I ◽  
Animal Feeding ◽  
Group Ii ◽  
Whole Cottonseed

Abstract To improve the efficiency of whole cottonseed (WCS) on dairy cattle, crush and alkali treatment were combined (Table 1) in this study. The gastrointestinal tract digestibility of WCS were measured by in situ ruminal incubation and in vitro fermentation, respectively. Finally, animal feeding experiment was conducted to evaluate the effect of processed WCS on dairy cattle. A total of 30 Holstein dairy cows with similar physiological status were allocated to three groups (CON, Group I, and Group II). Ration for the CON group was a basic ration with no WCS, ration with about 8% non-processed WCS was for Group I (DM basis), while the Group II was provided by the ration added with 8% Crush-Alkali (4% mixed alkali solids with NaOH/CaO ratio at 1:1) treated WCS. Results of the in situ and in vitro experiments showed that, compared with non-treated WCS, Crushing-Alkali treatment groups significantly improved WCS ruminal effective degradation rate of dry matter (DM), crude protein (CP), ether extract (EE), and neutral detergent fiber (NDF) (P < 0.05). Furthermore, Crush-Alkali (4% mixed alkali solids with NaOH/CaO ratio at 1:1) was the most beneficial to improve WCS gastrointestinal degradation of DM, CP and NDF, as well as enhance WCS rumen-bypass of EE. With the lowest free gossypol content (P< 0.05). Results of animal feeding experiment showed that DMI, 4% fat corrected milk production (4% FCM), milk protein, milk fat, and content of short-chain saturated fatty acids in milk in Group II significantly increased (P < 0.05) compared with CON group. DMI, ω-6 polyunsaturated fatty acids (PUFA) in milk, and the ratio of ω-6 to ω-3 PUFA were significantly higher in Group II than that in Group I (P < 0.05). Additionally, free gossypol concentration in serum and milk, as well as parameters reflecting liver function were not notably different among the three groups (P > 0.05).

scholarly journals Sources and sinks of acetone, methanol, and acetaldehyde in North Atlantic marine air

2005 ◽  
Vol 5 (7) ◽  
pp. 1963-1974 ◽  
Author(s):  
A. C. Lewis ◽  
J. R. Hopkins ◽  
L. J. Carpenter ◽  
J. Stanton ◽  
K. A. Read ◽  
...  
Keyword(s):  
North Atlantic ◽  
Degradation Mechanism ◽  
Chemical Degradation ◽  
Oh Radical ◽  
Detailed Model ◽  
Atmospheric Lifetime ◽  
Sources And Sinks ◽  
Marine Air ◽  
Made In

Abstract. Measurements of acetone, methanol, acetaldehyde and a range of non-methane hydrocarbons have been made in North Atlantic marine air at the Mace Head observatory. Under maritime conditions the combination of OVOCs (acetone, methanol and acetaldehyde) contributed up to 85% of the total mass of measured non methane organics in air and up to 80% of the OH radical organic sink, when compared with the sum of all other organic compounds including non-methane hydrocarbons, DMS and OH-reactive halocarbons (trichloromethane and tetrachloroethylene). The observations showed anomalies in the variance and abundance of acetaldehyde and acetone over that expected for species with a remote terrestrial emission source and OH controlled chemical lifetime. A detailed model incorporating an explicit chemical degradation mechanism indicated in situ formation during air mass transport was on timescales longer than the atmospheric lifetime of precursor hydrocarbons or primary emission. The period over which this process was significant was similar to that of airmass motion on intercontinental scales, and formation via this route may reproduce that of a widespread diffuse source. The model indicates that continued short chain OVOC formation occurs many days from the point of emission, via longer lived intermediates of oxidation such as organic peroxides and long chain alcohols.

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