Preserving protein homeostasis prevents motor impairment in DNA Damage Response-compromised C. elegans

To maintain genome integrity, cells rely on a complex system of DNA repair pathways and cell cycle checkpoints, together referred to as the DNA damage response (DDR). Impairments in DDR pathways are linked to cancer, but also to a wide range of degenerative processes, frequently including progressive neuropathy and accelerated aging. How defects in mechanistically distinct DDR pathways can drive similar degenerative phenotypes is not understood. Here we show that defects in various DDR components are linked to a loss of protein homeostasis in Caenorhabditis elegans. Prolonged silencing of atm-1, brc-1 or ung-1, central components in respectively checkpoint signaling, double strand break repair and base excision repair enhances the global aggregation of proteins occurring in adult animals, and accelerates polyglutamine protein aggregation in a model for neurodegenerative diseases. Overexpression of the molecular chaperone HSP-16.2 prevents enhanced protein aggregation in atm-1, brc-1 or ung-1-compromised animals. Strikingly, rebalancing protein homeostasis with HSP-16.2 almost completely rescues age-associated impaired motor function in these animals as well. This reveals that the consequences of a loss of atm-1, brc-1 or ung-1 converge on an impaired protein homeostasis to cause degeneration. These findings indicate that a loss of protein homeostasis is a crucial downstream consequence of DNA repair defects, and thereby provide an attractive novel framework for understanding the broad link between DDR defects and degenerative processes.

ADA PENGARUH YANG SIGNIFIKAN PEMBERIAN NECK CAILLIET EXERCISE TERHADAP PENURURAN NYERI LEHER PADA PEGAWAI ADMINISTRASI DI UNIT PELAKSANA TEKNIS DAERAH PUSKESMAS HILIDUHO TAHUN 2020

Neck pain is a collection of symptoms related to degenerative processes as well as those that are not related to degenerative processes such as poor positioning in job activity, direct trauma or repetitive micro-trauma. In helping to reduce neck pain in patients, the treatment given is neck cailliet exercise. To find out the influence of neck cailliet exercise to decrease neck pain in administration. This research is a quantitative study, a pseudo-experimental quasi by using the one group pre-Test post-Test design, having a sample of 18 respondents with a total sampling technique. Data analysis obtained using T-Test paired sample test which obtained the mean result prior to the administration neck Cailliet exercise is 5.50 with a standard deviation of 0.985 and the mean after giving neck cailliet exercise is 3.44 with a standard deviation 0.856, which means there is a decrease in neck pain after given the neck cailliet exercise with P value = 0.000 (P < 0.05). Result showed that the giving of neck cailliet exercise was influential for the reduction of neck pain in administration. Conclusion: giving neck cailliet exercise is very effective against reduction of neck pain in administration. Suggestion: The patient must re-apply the instruction given by the physiotherapy at home

Etiology and pathogenesis of depression.

Found a basic physiological need (X-need), a deficiency of which leads to depression. The cause of depression is a lack of fulfillment of the underlying physiological X-need. X-need is a powerful regulator of the balance of neurotransmitters and is associated with neurodegenerative processes in the body, ensures the normal functioning of physiological and mental processes. Chronic deficiency of X-need leads to degenerative processes in the body.

Clinical and Morphological Manifestations of Disorders of the Gastrointestinal Tract of Cats with Regular Consumption of Lactose

Annotation currently, in domestic cats (felis silvestris catus), degenerative processes of the intestine and stomach are widespread. The leading place among pathologies is occupied by conditions caused by the regular consumption of lactose. Veterinary gastroenterology as an independent area is a relatively young science, which is being improved from year to year. The increase in cases of visits to veterinary clinics of owners of cats with pathological conditions of the intestines and stomach causes increased professional interest among veterinarians of general practice, as well as the felinological community. The article presents the results of studies proving the important role of food antigens in the early formation of degenerative processes of the intestine and stomach, leading to a significant deterioration in the standard of living of the animal with a subsequent fatal outcome.

Adverse effects associated to the application of ultrasound-guided percutaneous needle electrolysis

Background Percutaneous needle electrolysis is a technique of invasive physical therapy which is increasingly used by physical therapists in their clinical practice. However, to date, no studies have analyzed the presence of adverse effects.The aim of the present study was to evaluate the incidence of adverse effects and the associated impact of the application of ultrasound-guided percutaneous needle electrolysis in disorders of the neuro-musculoskeletal system. Material and Method A prospective case series study was performed over a period of six months at the Sannus Clinic center (Madrid). A sample of patients was identified and recruited, and follow-up was performed up to six months after discharge. Initial information was collected regarding demographic data (age and sex) and clinical data (affected structure, area, type of pain and process associated to the pathology). During each of the sessions performed, percutaneous needle electrolysis was applied in an isolated manner and data were gathered on the treatment received, as well as the presence of any adverse effects. An adverse effect was considered as being any incident related with the application of percutaneous needle electrolysis which caused any damage, as perceived by both the patient and the physical therapist who applied the treatment. The type of adverse effect was recorded (pain, bleeding, hematoma, post-intervention vegetative reactions [sweating, pallor, abdominal discomfort], syncope, skin lesions, damage to organs, nerve lesions, pneumothorax, metal allergy), the moment these appeared (during application, after application, days after the application), its severity (transitory (<48h), reversible (resolved at discharge), irreversible), its impact (did not require any specific intervention, required an additional specific physical therapy intervention, required intervention from medical staff (without hospitalization), and cause (insufficient skill with the technique, malpractice, inappropriate protocol). The adverse effects were classified as mild or severe depending on whether or not an intervention was required. Results 214 patients (60.7% men; 39.3% women) received a total of 772 sessions, the mean number (and standard deviation) of sessions was 3.6 (1.1). The totality of patients treated with ultrasound-guided percutaneous electrolysis received more than one session, according to the methodology described by Valera & Minaya. The main reasons for consultation were tendinous pathologies (70.5%), muscle pathologies (11.7%), ligaments (6.5%), joint capsule-synovia (5.6%), nerve entrapments (4.2%) and others (1.4%). Degenerative processes were more common than acute inflammatory processes. The greatest incidence was in the lower limbs. Degenerative processes were significantly more frequent than tendinous problems. During the 772 sessions of ultrasound-guided percutaneous needle electrolysis, the most common adverse effects were pain during the intervention (96.1%) and in the days following treatment (71.1%), as well as mild vasovagal responses post-intervention (80.1%). One syncope was recorded (0.13%). All the effects were transitory and without impact. No hematomas were detected in the days after a mild bleeding, when this occurred (9.3%). Interventions were performed on the thorax in 1.5% of the procedures, close to organs (0.5%) or close to peripheral nerves (4.2%) without any adverse effect. In the 6-week follow-up after discharge no adverse effects were detected. Conclusions Percutaneous needle electrolysis is a safe technique. The adverse effects provoked by the application of percutaneous needle electrolysis are mild, transitory, without impact on the person's health and following a homogenous pattern. The pain and the mild vasovagal response associated with the intervention are frequent and inherent to the stimulus generated by the needling and the electric current employed.

Effectiveness of the Arthrofon in the treatment of dogs with knee osteoarthritis

The article describes the results of using the drug Arthrofon in complex postoperative treatment of rupture of the cranial cruciate ligament of the knee in dogs. The studies were conducted on two groups selected by the method of analogues. Based on the results of orthopedic examination, radiography and cytological studies of the synovial fluid of the knee joint, it was found that the use of the drug Arthrofon prevents the development of degenerative processes in the knee joint in the first 45 days after surgery. Animals on the background of the use of Arthrofon in complex therapy did not have X-ray signs of progression of osteoarthritis, and the synovial fluid recovered to normal values for this species.

Neuroprotective Potential of Ginkgo biloba in Retinal Diseases

Like other tissues of the central nervous system, the retina is susceptible to damage by oxidative processes that result in several neurodegenerative disease such as age-related macular degeneration, diabetic retinopathy, glaucoma, ischaemic retinal disease, retinal disease produced by light oxidation, and detached retina, among other diseases. The use of antioxidant substances is a solution to some health problems caused by oxidative stress, because they regulate redox homeostasis and reduce oxidative stress. This is important for neurodegeneration linked to oxidation processes. In line with this, Ginkgo biloba is a medicinal plant with excellent antioxidant properties whose effects have been demonstrated in several degenerative processes, including retinal diseases associated with neurodegeneration. This review describes the current literature on the role of ginkgo in retinal diseases associated with neurodegeneration. The information leads to the conclusion that G. biloba extracts might be a good option to improve certain neurodegenerative retinal diseases, but more research is needed to determine the safety and efficacy of G. biloba in these retinal degenerative processes.