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2022 ◽  
Vol 15 (1) ◽  
Author(s):  
Seo Young Kim ◽  
Jihye Choi ◽  
Junhee Roh ◽  
Chul Hoon Kim

AbstractIn the CNS, pericytes are important for maintaining the blood–brain barrier (BBB) and for controlling blood flow. Recently, several methods were suggested for the differentiation of human pluripotent stem cells (hPSCs) into brain mural cells, specifically pericytes or vascular smooth muscle cells (vSMCs). Unfortunately, identifying the pericytes from among such hPSC-derived mural cells has been challenging. This is due both to the lack of pericyte-specific markers and to the loss of defining anatomical information inherent to culture conditions. We therefore asked whether NeuroTrace 500/525, a newly developed dye that shows cell-specific uptake into pericytes in the mouse brain, can help identify human induced pluripotent stem cell (hiPSC)-derived brain pericyte-like cells. First, we found that NeuroTrace 500/525 specifically stains primary cultured human brain pericytes, confirming its specificity in vitro. Second, we found that NeuroTrace 500/525 specifically labels hiPSC-derived pericyte-like cells, but not endothelial cells or vSMCs derived from the same hiPSCs. Last, we found that neuroectoderm-derived vSMCs, which have pericyte-like features, also take up NeuroTrace 500/525. These data indicate NeuroTrace 500/525 is useful for identifying pericyte-like cells among hiPSC-derived brain mural cells.


Author(s):  
Hoang Van

Background: Percutaneous coronary angiography is considered the "gold standard" for the diagnosis of coronary artery disease and provides the necessary anatomical information to provide appropriate treatment. The limitation of coronary angiography is the accurate assessment of calcified coronary lesions. Intravascular ultrasonography has many advantages in the assessment of calcified coronary lesions. Methods: The descriptive clinical study. Evaluation of calcified coronary artery lesions by intravascular ultrasound Results: From January 2019 to December 2019, at the Hanoi Heart Institute, 64 patients had 64 coronary artery lesions surveyed by intravascular ultrasound. There were 42 (65,6%) calcified lesions assessed by IVUS and 25 (39,1%) calcified lesions were detected by coronary angiography. In addition, the location of calcified were revealed more in the LAD compared to other: LAD 60%, LCx 24%, RCA 12% and LM 4%. Conclusion: IVUS calcification detection rate is higher than coronary angiography. The most common site of calcification in the LAD.


Author(s):  
Quratulain Javaid

Palmaris longus is a muscle that is located in the anterior compartment of the forearm. Among the muscles belonging to the flexor compartment of forearm, palmaris longus is located at the most superficial position and that is why it is easy to access. It exhibits high variability and its prevalence ranges between 1.5% and 63.9%. The knowledge of prevalence of agenesis of palmaris longus is essential both in terms of updating anatomical information and also for physicians, radiologists, physiotherapists and surgeons. The surgeons must know about the variability as it may be beneficial while they plan surgeries involving the palmaris muscle as a graft. The current narrative review was planned to highlight the variability pattern of palmaris longus muscle in terms of prevalence, gender and laterality. Palmaris longus agenesis is more common in females and on the left side. Besides, unilateral agenesis is more common compared to bilateral agenesis.


2021 ◽  
Vol 9 ◽  
Author(s):  
Eva Chatzinikolaou ◽  
Kleoniki Keklikoglou ◽  
Panagiotis Grigoriou ◽  
Christos Arvanitidis

Digitisation of specimens (e.g. zoological, botanical) can provide access to advanced morphological and anatomical information and promote new research opportunities. The micro-CT technology may support the development of "virtual museums" or "virtual laboratories" where digital 3D imaging data are shared widely and freely. There is currently a lack of universal standards concerning the publication and curation of micro-CT datasets. The aim of the current project was to create a virtual gallery with micro-CT scans of individuals of the marine gastropod Hexaplex trunculus, which were maintained under a combination of increased temperature and low pH conditions, thus simulating future climate change scenarios. The 3D volume-rendering models created were used to visualise the structure properties of the gastropods shells. Finally, the 3D analysis performed on the micro-CT scans was used to investigate potential changes in the shell properties of the gastropods. The derived micro-CT 3D images were annotated with detailed metadata and can be interactively displayed and manipulated using online tools through the micro-CT virtual laboratory, which was developed under the LifeWatchGreece Research Infrastructure for the dissemination of virtual image galleries collection supporting the principles of FAIR data.


Author(s):  
Antonio Gangemi ◽  
Betty Chang ◽  
Paolo Bernante ◽  
Gilberto Poggioli

Since its advent, robotic surgery has redefined the operating room experience. It directly addressed and resolved many of the shortcomings of laparoscopic methods while maintaining a minimally invasive approach that brought benefits in cosmesis and healing for patients but also benefits in ergonomics and precision for surgeons. This new platform has brought with it changes in surgical training and education, principally through the utilization of virtual reality. Accurate depictions of human anatomy seen through augmented reality allow the surgeon-in-training to learn, practice and perfect their skills before they operate on their first patient. However, the anatomical knowledge required for minimally invasive surgery (MIS) is distinct from current methods of dissection and prosection that inherently cater towards open surgery with large cuts and unobstructed field. It is integral that robotic surgeons are also equipped with accurate anatomical information, heralding a new era in which anatomists can work alongside those developing virtual reality technology to create anatomical training curricula for MIS. As the field of surgery and medicine in general moves to include more and more technology, it is only fitting that the building blocks of medical education follow suit and rediscover human anatomy in a modern context.


2021 ◽  
pp. 51-64
Author(s):  
CHAN-GYU YUN

A right frontal bone belonging to the tyrannosaurid theropod Teratophoneus curriei from the Campanian Kaiparowits Formation of Utah provides important anatomical information that is useful in understanding tyrannosaurid taxonomy and relationships. Many aspects of its anatomy indicate a subadult ontogenetic status of this individual, as they compare favorably with subadults of other tyrannosaurids such as Daspletosaurus torosus and Tyrannosaurus rex. This is consistent with the previous interpretation that this individual was subadult, based on different cranial bones such as lacrimal or maxilla. It is likely that a previous assessment of the body mass of this individual was underestimated, and it is presumed here that it was most likely around 1000 kg. This description of the frontal anatomy has several implications for previous works on tyrannosaurid cranial anatomy, and information that corrects aspects of our understanding on tyrannosaurid frontal anatomy is provided. Reanalysis of Nanuqsaurus hoglundi, a tyrannosaurid theropod that has similar frontal morphology with Teratophoneus curriei, implies that parts of its diagnosis are problematic and the proposed evidence for the supposed diminutive body size of this taxon is weak.


Author(s):  
Shraddha P. Diwalkar

Abstract: Medical image fusion is the technique of integrating two or more images from various imaging modalities/scans to get a fused image with information having the details of anatomical information combined from all the modalities for accurate diagnosis and further treatment. This paper performs the analysis of various wavelet functions for decomposition and synthesis. PET (Positron Emission Tomography) and MRI (Magnetic Resonance Imaging) scans of Brain and chest are used and compared using Stationary Wavelet Transform (SWT) and Discrete wavelet Transform (DWT). Entropy is calculated which is a measure of information acquired after the fusion process. Keywords: Wavelet transform, Fusion, Stationary Wavelet Transform, Discrete, Medical image


2021 ◽  
Vol 17 (4) ◽  
pp. 1-24
Author(s):  
Wen Xie ◽  
Zeyang Yao ◽  
Erchao Ji ◽  
Hailong Qiu ◽  
Zewen Chen ◽  
...  

Congenital heart disease (CHD) is the most common birth defect, accounting for one-third of all congenital birth defects. As with complicated intracardiac structural abnormalities, CHD is usually treated with surgical repair, and computed tomography (CT) is the main examination method for diagnosis of CHD and also provides anatomical information to surgeons. Currently, there exists a serious shortage of professional surgeons in developing countries. Compared with developed countries where large hospitals and cardiovascular disease centers have professional surgical teams with rich treatment experience, surgeons in developing countries and remote areas suffer from lack of professional surgical skills resulting with low surgical quality and high mortality. Recently, surgical telementoring has been popular to tackle the above problems, in which less-skilled surgeons can get real-time guidance from skilled surgeons remotely through audio and video transmission. However, there still exists difficulties in applying telementoring to CHD surgeries including high resource consumption on medical data transmission and storage, large image noise, and inconvenient and inefficient discussion between surgeons on CT. In this article, we proposed a framework with an image compression module, an image denoising module, and an image segmentation module based on CT images in CHD. We evaluated the above three modules and compared them with existing works, respectively, and the results show that our methods achieve much better performance. Furthermore, with 3D printing, VR technology, and 5G communications, our framework was successfully used in a real case study to treat a patient who needed surgical treatment.


Author(s):  
Zuzilla ◽  
T.Mohd Yoshandi ◽  
Danil Hulmansyah

Columna cervical vertebrae are a type of long bone that occurs on the back of the head. One of the radiological examinations most frequently performed in the hospital is the examination of the right posterior oblique projection cervical vertebrae (RPO). However, to see the cervical vertebrae column radiograph that is more optimal for cases of HNP, fractures, fissures, root symdrome. with a variation of the angle of 15 ° and 20 ° cranially. To find out which method is the most informative to display the right posterior oblique (RPO) projection with an angle of 15 ° and 20 ° cranially. This research is a type of quantitative research with statistical expansion. This research uses phantom. The research was conducted at the STIKes Awal Bros Pekanbaru in August 2020. The vertebral column results were used well, namely at an angle of 15 ° with clearly visible vertebral foramen, clearly visible intervertebral disc, visible C4 - C7, spinous process, clearly visible intervertebral disc.


Diagnostics ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1841
Author(s):  
Kyungsoo Bae ◽  
Kyung-Nyeo Jeon

Dual-energy computed tomography (CT) is a promising tool, providing both anatomical information and material properties. Using spectral information such as iodine mapping and virtual monoenergetic reconstruction, dual-energy CT showed added value over pulmonary CT angiography in the diagnosis of pulmonary embolism. However, the role of non-contrast-enhanced dual energy CT in pulmonary embolism has never been reported. Here, we report a case of acute pulmonary embolism detected on an electron density image from an unenhanced dual-energy CT using a dual-layer detector system.


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