Elucidation of The Effects and Underlying Mechanism of Aerobic Interval Training Combined With Liraglutide On Diabetic Cardiomyopathy

objective: This study intended to explore the hypoglycemic and cardioprotective effects of 8-week aerobic interval training combined with liraglutide and elucidate the underlying mechanisms.Method: Male Wistar rats were randomly divided into 5 groups - normal control (CON), diabetic cardiomyopathy (DCM), high-dose liraglutide (DH), low-dose liraglutide DL , and aerobic interval training combined with liraglutide (DLE). The cardiac function of rats ,the FBG the levels of fasting insulin (FIN), HbA1c, the total collagen content , AGEs, the mRNA expression of myocardial remodeling genes BNP, GSK3β, α-MHC, and β-MHC ,the expression of GLP-1 and GLP-1R proteins, Insulin resistance (HOMA-IR) and beta-cell function (HOMA-β) was analyze. Results: During the intervention, the FBG in each intervention group significantly decreased compared to the DCM group. After 8 weeks,the DH, DL, and DLE groups showed improved blood glucose-related indices and cleared the accumulated AGEs in the DCM groups. The heart function in the DLE groups was significantly improved than that in the DH and DL groups. The relative expression of BNP mRNA in the DH, DL, and DLE groups significantly reduced compared to the CON and the DCM group .Compared to the DCM group,the relative expression of α-MHC mRNA increased significantly and β-MHC mRNA decreased notably in the myocardium of the DH, DL, and the DLE group.The expression of GLP-1 in the myocardial tissue of rats in the DH group was higher than that in the DL and DLE groups. GLP-1R expression in the myocardial tissue in the DLE group was higher than that in the DH , DL and the DCM groups .Conclusion: Liraglutide combined with AIT intervention significantly reduced FBG and the fluctuations in FBG, alleviated myocardial fibrosis, improved cardiac function in DCM rats, supporting the efficacy of the combined pharmaceutical and physical intervention, and reduced the cost of treatment.

Aerobic interval training improves irisin and chemerin levels of both liver and visceral adipose tissues and circulating asprosin in rats with metabolic syndrome

The perturbation of adipokinetic hormones, such as irisin, chemerin, and asprosin has been reported to participate in pathological conditions (e.g., insulin resistance) and chronic inflammation. However, exercise training has been long established as an effective intervention for prevention and treatment of these chronic and metabolic diseases. This study was to examine the effects of aerobic continuous training (ACT) and aerobic interval training (AIT) on irisin and chemerin levels of liver tissue (LT) and visceral adipose tissue (VAT), circulating asprosin, and their relationships with cardiometabolic risk factors in rats with metabolic syndrome (MetS). Thirty-two male Wistar rats were randomly divided into four equal groups: normal control (N-Ctr), control (Ctr-MetS), ACT, and AIT. After familiarization, rats with exercise intervention performed either ACT or AIT five times a week over eight weeks. The level of irisin in both ACT and AIT groups was higher than the Ctr-MetS group in LT and VAT, with a greater improvement of LT level observed in AIT vs. ACT groups. Furthermore, the level of chemerin in LT and VAT was lower in both ACT and AIT groups than the Ctr-MetS group, whereas only AIT group exhibited a reduction of serum asprosin when compared to ACT and Ctr-MetS, along with the improvements of cardiometabolic markers, such as HOMA-IR and lipid profile. These findings may support the efficiency and effectiveness of AIT intervention in the modulation of these novel metabolic hormones and cardiometabolic risk factors for reduced risk of metabolic syndrome.

Efficacy of Vitamin D Supplementation in Addition to Aerobic Exercise Training in Obese Women with Perceived Myalgia: A Single-Blinded Randomized Controlled Clinical Trial

Obese women were more susceptible to myalgia because of their significantly lower vitamin D concentrations; the present study investigated the efficacy of vitamin D in addition to an aerobic interval training in the management of obese women with myalgia. Forty-five obese women with vitamin D deficiency and myalgia (30 to 40 years old) were assigned randomly into three equal groups. Group A received an aerobic interval training with vitamin D supplementation, Group B received vitamin D supplementation only, and Group C received aerobic interval training only; participants in all groups were on calorie deficient diets. The study outcomes were the Visual Analog Scale (VAS) for Pain Evaluation, serum vitamin D level, and Cooper 12-Minute Walk Test for Functional Capacity Evaluation, while the Short-Form Health Survey (SF) was used for assessment of quality of life. We detected a significant improvement in pain intensity level, serum vitamin D level, and quality of life in all groups with significant difference between Group A and groups B and C. We also detected a significant improvement in functional capacity in groups A and C, with no significant change in Group B. Aerobic interval training with vitamin D supplementation was more effective for the management of obese women with perceived myalgia.

Effect of Eight Weeks of Aerobic Interval Training on the Serum Concentrations of Alkaline Phosphatase, Osteocalcin and Parathyroid Hormone in Middle-aged Men

Background: Osteoporosis is the most common disorder of bone and mineral metabolism. This disease is associated with decreased bone density and loss of bone microstructure, leading to increased fragility and risk of bone fractures. Objectives: The aim of this study was to evaluate the effect of eight weeks of aerobic interval training on the serum concentrations of alkaline phosphatase, osteocalcin, and parathyroid hormone in middle-aged men. Methods: This was a quasi-experimental study of 24 men, who were divided into two groups of control (n = 12) and aerobic interval training (n = 12). Training performed during eight weeks, three times a week, each session for 45 to 60 minutes. Blood samples were analyzed for the concentrations of calcium, phosphorus, alkaline phosphatase, osteocalcin, and parathyroid hormone before and after completion of the training program. Paired samples t-test and analysis of covariance (ANCOVA) were run to analyze the data (P ≤ 0.05). Results: Weight and body mass index decreased significantly, and the maximum oxygen consumption at the end of the training program increased significantly in the experimental group. Eight weeks of aerobic exercise led to a significant increase in the amounts of calcium (P = 0.001), phosphorus (P = 0.001), alkaline phosphatase (P = 0.001), osteocalcin (P = 0.001), and parathyroid hormone (P = 0.001) in the experimental group. Conclusions: It can be stated that eight weeks of aerobic interval training may be effective in preventing osteoporosis by reducing body composition indices and increasing calcium, phosphorus, alkaline phosphatase, osteocalcin, and serum parathyroid hormone in inactive men.

Digoxin Combined with Aerobic Interval Training Improved Cardiomyocyte Contractility

Digoxin is a cardiotonic that increases the cardiac output without causing deleterious effects on heart, as well as improves the left ventricular performance during physical exercise. We tested whether the association between chronic digoxin administration and aerobic interval training (AIT) promotes beneficial cardiovascular adaptations by improving the myocardial contractility and calcium (Ca2+) handling. Male Wistar rats were randomly assigned to sedentary control (C), interval training (T), sedentary digoxin (DIGO) and T associated to digoxin (TDIGO). AIT was performed on a treadmill (1h/day, 5 days/week) for 60 days, consisting of successive 8-min periods at 80% and 20% of VO2máx for 2 min. Digoxin was administered by orogastric gavage for 60 days. Left ventricle samples were collected to analysis of Ca2+ handling proteins; contractility and Ca2+ handling were performed on isolated cardiomyocytes. TDIGO group had a greater elevation in fractional shortening (44%) than DIGO, suggesting a cardiomyocyte contractile improvement. In addition, T or TDIGO groups showed no change in cardiomyocytes properties after Fura2-acetoxymethyl ester, as well as in sarcoplasmic reticulum Ca2+-ATPase (SERCA2a), phospholamban and calcineurin expressions. The main findings indicate that association of digoxin and aerobic interval training improved the cardiomyocyte contractile function, but these effects seem to be unrelated to Ca2+ handling.

Comparative efficiency of continuous trainings and aerobic interval training in patients after myocardial infarction

Background The basis of cardiac rehabilitation programs (CRP) is aerobic physical training, which improves exercise capacity in patients with an acute myocardial infarction (MI) after primary angioplasty of the infarct-related vessel. However, which type - aerobic interval training (AIT) or aerobic continuous trainings (ACT) are most effective for improving peak oxygen uptake (VO2 max) remains unclear and controversial. Purpose To evaluate the effects of CRP, including AIT or ACT, on exercise capacity and lung function in patients with MI after primary angioplasty of the infarct-related vessel. Methods Seventy MI patients (58 men and 12 women, mean age – 59,2±8,0 years) were undergone the three-week CRP in the Ivanovo State Medical Academy Clinic. The CRP for all patients included: daily controlled physical training on the Bicycle Ergometer and Treadmill, group exercises in therapeutic gymnastics, dosed walking and walking on the stairs with an individually calculated pace. Depending on the mode of physical training all patients were randomized of five weekly sessions in to two groups, comparable in age and gender: AIT group (35 patients) and ACT group (35 patients). The ACT group was training at 50–60% of peak heart rate. The AIT group protocol consisted of ten 1-min intervals at 85–90% of maximal heart rate separated by ten 1-min active recovery periods of moderate intensity at 40–60% of the maximal heart rate. Changes in 6-min walk distance test (6MWT), peak oxygen uptake (VO2max), relative load power, total lung capacity (TLC) and forced expiratory volume in one second (FEV1) before and after CRP were investigated. Data are presented as a median (interquartile ratio [Q1; Q3]). Results After finishing CRP 6MWT distance increased in both groups, but was higher in AIT group 492 [460–510] m compared to the ACT group – 465 [424–510] m (p<0.05). Peak oxygen uptake increased more (p<0.05) after AIT: from 16.8 [15.3–19.8] to 20.7 [16.5–21.9] ml kg–1 min–1 (p<0.05), than after ACT – from 15.8 [14.7–18.6] to 16.1 [15.8–20.5] ml kg–1 min–1 (p>0.05). At the end of the CRP relative load power during cardiopulmonary exercise test was higher in AIT group compared to the ACT group: 1.5 [1.3–1.7] vs 1.2 [1.0–1.5] Wt/kg respectively (p<0.05). In the AIT group TLC increased significantly from 3.13 [2.63–4.05] to 4.14 [3.91–4.87] L (p<0.05) then in ACT group: from 3.19 [2.82–3.74] to 3.21 [3.03–3.57] L (p>0.05). FEV1 had no changes in both groups. Both programs reduced anxiety and depression, systolic and diastolic blood pressure and increased quality of life. Conclusion AIT as compared to ACT provided a more pronounced training effect on the cardiorespiratory system due to high-intensity loading phases. Therefore, AIT is more preferable in cardiac rehabilitation of patients with MI after primary angioplasty of the infarct-related vessel. Funding Acknowledgement Type of funding source: Public Institution(s). Main funding source(s): Ivanovo State Medical Academy

Efecto de un programa de entrenamiento interválico aeróbico de alta intensidad en población escolar femenina con sobrepeso u obesidad (Effect of a high intensity aerobic interval training program on an overweight or obese female school population)

El presente estudio de alcance explicativo, realizado bajo un diseño cuasiexperimental de preprueba-posprueba y grupo control, determinó el efecto de un programa de entrenamiento interválico aeróbico de alta intensidad (HIIT) que utilizó juegos motores y circuitos sociomotrices, durante 16 semanas (tres sesiones semanales) para el incremento del consumo de oxígeno pico (VO2pico) y disminución del índice de masa corporal (IMC) y porcentaje de grasa (%GC) en niñas con sobrepeso u obesidad de 10 y 11 años de la sede Marino Dávalos de la Institución Educativa Moderna de Tuluá, distribuidas en dos grupos, experimental (n=8) y control (n=8). Entre los resultados más importantes, se destaca el aumento del VO2pico en 3.6 ml/kg/min, la disminución del IMC en 1.62 y del %GC donde se apreció una disminución del 3.72% en el grupo experimental; mientras que en el grupo control, no se observaron modificaciones estadísticamente significativas; concluyendo el estudio que el programa HIIT que utilizó como medio los juegos motores y circuitos sociomotrices, presentó efectos significativos en el incremento del VO2pico y disminución del IMC y el %GC de las niñas con sobrepeso u obesidad, validando de esta manera la hipótesis de investigación, con niveles de significancia estadística (p< .05) para la prueba de muestras relacionadas (p= .000 para el VO2pico, p=.000 para %GC y p=.000 para IMC). Abstract. The present explanatory scope study, carried out under a quasi-experimental design of pretest-posttest and control group, determined the effect of a high intensity aerobic interval training (HIIT) program that used motor games and sociomotor circuits, for 16 weeks (three sessions weekly) for the increase in peak oxygen consumption (VO2peak) and decrease in body mass index (BMI) and percentage of fat (% GC) in overweight or obese girls of 10 and 11 years of age from the Marino Dávalos headquarters of the Institution Educativa Moderna de Tuluá, divided into two groups, experimental (n = 8) and control (n = 8). Among the most important results, the increase in VO2peak by 3.6 ml/kg/min, the decrease in BMI by 1.62 and the% GC where a decrease of 3.72% was observed in the experimental group stand out; while in the control group, no statistically significant changes were observed; The study concluded that the HIIT program that used motor games and sociomotor circuits as a means, presented significant effects in the increase of the VO2peak and decrease of the BMI and the% GC of the girls with overweight or obesity, thus validating the research hypothesis. , with levels of statistical significance (p <.05) for the test of related samples (p = .000 for VO2peak, p = .000 for % GC and p = .000 for BMI).