Contribution of tiller age category to herbage accumulation of Marandu palisadegrass under two fertilization regimes

Based on the hypothesis that climate and fertilizers influence the proportion of tiller age categories in the canopy and, consequently, in herbage accumulation, the objective of this study was to determine the population density and the contribution of tiller age categories for herbage accumulation of Urochloa brizantha cv. Marandu during the year and in response to phosphate and nitrogen fertilization. The treatments consisted of two fertilization strategies: low and high fertilization and three tiller ages (young, mature and old), evaluated at four times of the year: winter, early and late spring, and summer. The total number of tillers and the proportion of young tillers were higher in late spring and summer. The growth and herbage accumulation rates showed a typical seasonal pattern and were higher in the canopy under high fertilization. Old tillers contributed more to control the total stem growth rate, as well as the canopy senescence rate. The highest percentage of young tillers is related to the high herbage accumulation in the Marandu palisadegrass canopy.

Thermal conditions predict intraspecific variation in senescence rate in frogs and toads

Variation in temperature is known to influence mortality patterns in ectotherms. Even though a few experimental studies on model organisms have reported a positive relationship between temperature and actuarial senescence (i.e., the increase in mortality risk with age), how variation in climate influences the senescence rate across the range of a species is still poorly understood in free-ranging animals. We filled this knowledge gap by investigating the relationships linking senescence rate, adult lifespan, and climatic conditions using long-term capture–recapture data from multiple amphibian populations. We considered two pairs of related anuran species from the Ranidae (Rana luteiventris and Rana temporaria) and Bufonidae (Anaxyrus boreas and Bufo bufo) families, which diverged more than 100 Mya and are broadly distributed in North America and Europe. Senescence rates were positively associated with mean annual temperature in all species. In addition, lifespan was negatively correlated with mean annual temperature in all species except A. boreas. In both R. luteiventris and A. boreas, mean annual precipitation and human environmental footprint both had negligible effects on senescence rates or lifespans. Overall, our findings demonstrate the critical influence of thermal conditions on mortality patterns across anuran species from temperate regions. In the current context of further global temperature increases predicted by Intergovernmental Panel on Climate Change scenarios, a widespread acceleration of aging in amphibians is expected to occur in the decades to come, which might threaten even more seriously the viability of populations and exacerbate global decline.

Cellular Senescence Is Associated with Faster Progression of Focal Segmental Glomerulosclerosis

<b><i>Background:</i></b> A current, albeit unproven, hypothesis is that an acceleration of cellular senescence is involved in impaired renal repair and progression of glomerular diseases. Focal segmental glomerulosclerosis (FSGS) is a glomerular disease with a substantial risk for progression to ESRD. However, if and to what extent cell senescence predicts a negative outcome in FSGS is still unknown. <b><i>Methods:</i></b> The hypothesis that cell senescence represents a proximate mechanism by which the kidney is damaged in FSGS (NOS phenotype) was investigated in 26 consecutive kidney biopsies from adult FSGS cases (eGFR 72 ± 4 mL/min, proteinuria 2.3 ± 0.6 g/day) who were incident for 2 years in a Northern Italian nephrology center and had a 6-year clinical follow-up. <b><i>Results:</i></b> Cell senescence (p16^INK4A, SA-β-galactosidase [SA-β-Gal]) was upregulated by ∼3- to 4-fold in both glomerular and tubular cells in kidney biopsies of FSGS as compared to age-matched controls (<i>p</i> &#x3c; 0.05–0.01). Tubular SA-β-Gal correlated with proteinuria and glomerulosclerosis, while only as a trend, tubular p16^INK4A was directly associated with interstitial fibrosis. At univariate analysis, basal eGFR, proteinuria, and tubular expression of SA-β-Gal and p16^INK4A were significantly directly related to the annual loss of eGFR. No correlation was observed between glomerular p16^INK4A and eGFR loss. However, at multivariate analysis, eGFR, proteinuria, and tubular p16^INK4A, but not SA-β-Gal, contributed significantly to the prediction of eGFR loss. <b><i>Conclusions:</i></b> The results indicate that an elevated cell senescence rate, expressed by an upregulation of p16^INK4A in tubules at the time of initial biopsy, represents an independent predictor of progression to ESRD in adult patients with FSGS.

Growth of Megathyrsus maximus cv. Mombaça as affected by grazing strategies and environmental seasonality. II. Dynamics of herbage accumulation

For tropical forage grasses, leaves are the main morphological component accumulated at the beginning of regrowth, and as leaf area index increases, plants change their growth pattern, allocating more resources to stem elongation as a means of optimising light capture. We hypothesised that, for Mombaça guineagrass, stem elongation and leaf senescence rate play a major role in determining net herbage accumulation rate (NHAR) and that senescence would be the key factor driving the reduction in NHAR when regrowth is interrupted beyond the critical leaf area index, that is, when canopy light interception (LI) exceeds 95%. Treatments corresponded to all combinations of two levels of pre-grazing canopy LI during regrowth (LI95% and LIMax) and two post-grazing heights (30 and 50 cm), and were allocated to experimental units according to a randomised complete block design with four replications. Measurements were performed throughout contrasting climatic conditions during four seasons from January 2001 to February 2002. Overall, spring and the two summers were the seasons when rates of leaf appearance, leaf elongation and leaf growth rate were higher, and leaf lifespan was smaller; the inverse occurred during autumn–winter. Despite the lack of statistically significant differences in NHAR between LI treatments during some seasons of the year, the ratio of leaf growth rate to NHAR was consistently higher for LI95% than LIMax. The pre-grazing target of LI95% was also efficient for decreasing stem elongation and leaf senescence rate. Increased senescence on individual tillers was the key driver generating modifications in the patterns of variation in NHAR beyond the critical leaf area index, suggesting that there is no advantage in extending the regrowth period when the main objective is to maximise leaf growth rate or NHAR.

Morphogenetic and structural characteristics of gamba grass subjected to nitrogen fertilization and different defoliation intensities

T This study aimed to evaluate the morphogenetic, structural, and productive characteristics of Andropogon gayanus cv. ‘Planaltina’ subjected to different nitrogen fertilization levels and defoliation intensities. The experiment was done in a completely randomized design with a factorial arrangement of six nitrogen doses (0, 100, 200, 300, 400, and 500 kg N ha-1) and two defoliation intensities (15 and 30 cm). The production and structure data were grouped into rainy and dry periods, while morphogenesis data were compared only among different fertilization and defoliation intensities. The total dry forage biomass (TDFB), dry mass of leaves (DML), dry mass of stems (DMS), and dead forage dry mass (DFDM) were then determined. In addition to these variables, the morphogenetic characteristics of the plants and the numbers of tillers alive and dead were evaluated. There was no interaction (P>0.05) between the effects of nitrogen fertilization doses and the intensity of defoliation on TDFB, DML, DMS, and DFDM in both of the evaluated phases (rainy and dry). It was also observed that the rates of leaf elongation, leaf appearance, and leaf blade elongation increased with nitrogen fertilization. On the other hand, the culm elongation rate and phyllochron decreased as the dose applied increased. The defoliation intensity did not influence (P>0.05) the morphogenetic characteristics examined, nor did it affect the senescence rate of nitrogen in leaves and number of live leaves per tiller. The number of dead tillers increased linearly during the rainy period. Based on these results, to improve the efficiency of production of gamba grass it is recommended that it be managed with a defoliation intensity of 30 cm and nitrogen fertilization of 286.52 kg N ha-1 year-1.

Morphogenesis, structure, and dynamics of paiaguas palisadegrass tillering after intercroping with sorghum for the recovery of pasture in different forage systems

The use of pasture is the most economical alternative for feeding ruminants. However, most pastures in Central Brazil are experiencing some degree of degradation. Crop-livestock integration has been shown to be a sustainable and viable alternative for the recovery of these degraded areas. The objective of this study was to evaluate the morphogenic and structural characteristics of Paiaguas palisadegrass and to determine the population dynamics and the survival rate of the tillers, through a stability diagram of the grass, after intercropping with sorghum for the recovery of pastures in different, integrated, crop-livestock forage systems. The experimental design was a randomized block design, with four replications. The treatments were composed of four forage systems: Paiaguas palisadegrass monoculture, sorghum intercropped with Paiaguas palisadegrass in the row, sorghum intercropped with Paiaguas palisadegrass in the interrow, and sorghum intercropped with oversown Paiaguas palisadegrass. The evaluations were performed in the four climatic seasons of the year (winter, spring, summer, and fall) in the same plots during the period of one year in 16 1042 m2 enclosures under a continuous stocking system. No effect was observed for the forage systems and seasons of the year on the leaf elongation rate, leaf senescence rate, and leaf life span. The development of Paiaguas palisadegrass was reduced in the winter relative to the other seasons of the year. However, it presented satisfactory production during this period despite the low rainfall. The results showed that the Paiaguas palisadegrass showed satisfactory production in all forage systems and may be indicated for intercropping with sorghum to promote the recovery and formation of pastures. The use of crop-livestock integration systems is an efficient cultivation technique for the recovery and formation of low-cost pastures to meet the demands of animal production.

The Sensitivity of a Honeybee Colony to Worker Mortality Depends on Season and Resource Availability

Background: Honeybees have extraordinary phenotypic plasticity in their senescence rate, making them a fascinating model system for the evolution of aging. Seasonal variation in senescence and extrinsic mortality results in a tenfold increase in worker life expectancy in winter as compared to summer. To understand the evolution of this remarkable pattern of aging, we must understand how individual longevity scales up to effects on the entire colony. We develop a matrix model of colony demographics to ask how worker age-dependent and age-independent mortality affect colony fitness and how these effects differ by seasonal conditions.Results: We find that there are seasonal differences in honeybee colony sensitivity to both senescent and extrinsic worker mortality. Colonies are most sensitive to extrinsic (age-independent) nurse and forager mortality during periods of higher extrinsic mortality and resource availability but most sensitive to age-dependent mortality during periods of lower extrinsic mortality and lower resource availability.Conclusions: These results suggest that seasonal changes in the strength of selection on worker senescence partly explain the observed pattern of seasonal differences in worker aging in honey bees. More broadly, these results extend our understanding of the role of extrinsic mortality in the evolution of senescence to social animals.

Morphogenesis of Cynodon cultivars fertilized with nitrogen

To evaluate the biomass flow of two cultivars of Cynodon (Tifton 85 grass and vaquero grass) fertilized with increasing levels of nitrogen (N) (control – without nitrogen; 200; 400 and 600 mg dm-3) under greenhouse conditions, this study was carried out, in a completely randomized design, with factorial arrangement. Forages were examined during three regrowth cycles, with results presented as mean values of the cycles. In the analysis of the effect of nitrogen fertilization, the Tifton 85 grass showed an increasing response to nitrogen levels for the leaf elongation rate (LER). The vaquero grass had a quadractic response for the LER with increasing levels of N. As for the stem elongation rate, there was an increasing linear response in both grasses according to increasing levels of N. The leaf senescence rate (LSR) of the Tifton 85 grass has been enhanced by nitrogen fertilization. The vaquero grass revealed a quadractic response for this variable LSR with nitrogen fertilization, with a minimum value of 0.96 cm tiller-1 day-1 with nitrogen level of 42.5 mg dm-3. The phyllochron and the total number of leaves of both grasses have been influenced by nitrogen fertilization, with downward and upward linear responses, respectively, with increasing levels of nitrogen. For the final mean leaf length (MLL) of both forages, we verified an increasing response with increasing levels of nitrogen. Tifton 85 grass and vaquero grass presented similar LER in the absence of nitrogen, but higher values were observed for the fertilized Tifton 85 grass. The fertilization using nitrogen leads to positive responses of the biomass flow of Tifton 85 grass and vaquero grass under the conditions of the present study.

The morphogenesis of marandu palisadegrass at fixed or variable heights in different seasons of the year

Height variations in a sward over the year may be efficient for increasing plant growth, compared with maintenance of the sward at a constant height. Thus, this experiment was conducted from February 2013 to May 2014 to characterise the development of Urochloa brizantha syn. Brachiaria brizantha cv. Marandu (marandu palisadegrass), managed under three defoliation strategies: constant height (30 cm during the entire experimental period), increasing height (15 cm in the winter, 30 cm in the spring, and 45 cm in the summer), and decreasing height (45 cm in the winter, 30 cm in the spring, and 15 cm in the summer). The experimental design was completely randomised, in a split-plot arrangement, with four replicates. Phyllochron was higher in the winter, but leaf and stem elongation rates and the number of live leaves per tiller were lower compared with the spring and summer. In the summer, the swards under increasing height displayed a higher phyllochron than those under decreasing and constant height. When comparing seasons, the highest leaf senescence rate occurred in spring. In the winter, the sward with increasing height had shorter leaves and stems, in contrast to the summer. The sward under decreasing height showed a high stem elongation rate in the spring. Marandu palisadegrass has great flexibility in terms of defoliation management and a typical seasonal development pattern. Modifying the sward height results in a gradual change in the development of marandu palisadegrass and generates residual effects on the subsequent season.

Unraveling LMNA Mutations in Metabolic Syndrome: Cellular Phenotype and Clinical Pitfalls

This study details the clinical and cellular phenotypes associated with two missense heterozygous mutations in LMNA, c.1745G>T p.(Arg582Leu), and c.1892G>A p.(Gly631Asp), in two patients with early onset of diabetes mellitus, hypertriglyceridemia and non-alcoholic fatty liver disease. In these two patients, subcutaneous adipose tissue was persistent, at least on the abdomen, and the serum leptin level remained in the normal range. Cellular studies showed elevated nuclear anomalies, an accelerated senescence rate and a decrease of replication capacity in patient cells. In cellular models, the overexpression of mutated prelamin A phenocopied misshapen nuclei, while the partial reduction of lamin A expression in patient cells significantly improved nuclear morphology. Altogether, these results suggest a link between lamin A mutant expression and senescence associated phenotypes. Transcriptome analysis of the whole subcutaneous adipose tissue from the two patients and three controls, paired for age and sex using RNA sequencing, showed the up regulation of genes implicated in immunity and the down regulation of genes involved in development and cell differentiation in patient adipose tissue. Therefore, our results suggest that some mutations in LMNA are associated with severe metabolic phenotypes without subcutaneous lipoatrophy, and are associated with nuclear misshaping.