scholarly journals Investigating the Production of Antimicrobial Nanoparticles by Chlorella vulgaris and the Link to Its Loss of Viability

2022 ◽  
Vol 10 (1) ◽  
pp. 145
Author(s):  
Munirah F. Aldayel ◽  
Mayyadah A. Al Kuwayti ◽  
Nermin A. H. El Semary
Keyword(s):  
Silver Nitrate ◽  
Chlorella Vulgaris ◽  
Antibacterial Activities ◽  
Prolonged Incubation ◽  
Synthesis Of Nanoparticles ◽  
Nanosilver Particles ◽  
Antimicrobial Nanoparticles ◽  
Scanning Electronmicroscopy ◽  
Banding Profile ◽  
Uv Visible

Chlorella vulgaris from Al-Ahsa, KSA was proved to be an active silver and gold nanoparticle producer. Nanogold and nanosilver particles were characterized using UV-visible spectroscopy, Fourier-transform infrared spectroscopy, and scanning electronmicroscopy. Both nanoparticles were used in the antimicrobial bioassay. The two nanoparticles showed antibacterial activities, with the silver nanoparticles being the most effective. To investigate the argumentative nature of their biosynthesis (i.e., whether it is a biotic or abiotic process), we isolated total ribonucleic acid RNA as an indicator of vitality. RNA was completely absent in samples taken after one week of incubation with silver nitrate and even after one or two days. However, successful extraction was only achievable in samples taken after incubation for one and four hours with silver nitrate. Most importantly, the gel image showed recognizable shearing of the nucleic acid after 4 h as compared to the control. An assumption can be drawn that the synthesis of nanoparticles may start biotically by the action of enzyme(s) and abiotically by action of reducing entities. Nonetheless, with prolonged incubation, excessive nanoparticle accumulation can be deadly. Hence, their synthesis continues abiotically. From the RNA banding profile, we suggest that nanosilver production starts both biotically and abiotically in the first few hours of incubation and then continues abiotically. Nanosilver particles proved to have more of an antimicrobial impact than nanogold and hence are recommended for different applications as antibacterial agents.

Study of Antibacterial Activity of Nanosilver-Polypropylene Composite against Contaminated Bacteria in Molasses

2018 ◽  
Vol 939 ◽  
pp. 163-169
Author(s):  
Kittiya Pongsapasiritat ◽  
Nongnapat Jumnienkul ◽  
Rapeepun Dangtungee ◽  
Malinee Sriariyanun
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Research Field ◽  
Total Sugar ◽  
Bioethanol Production ◽  
Polypropylene Composite ◽  
Nanosilver Particles ◽  
Sugar Degradation

The nanosilver particles are attractive in the research field of nanotechnology due to one of their applications as an effective antibacterial agent. The main objective of the current research is to inhibit the bacterial growth in the molasses to decrease the rate of total sugar degradation caused by contaminated bacteria. The polypropylene masterbatch containing with AgNPs (PP/AgNPs) was prepared and tested against contaminated bacterial species identified in molasse. The percentage of bacterial inhibition was calculated by observing the viability of bacteria cultures by using spread plate technique after treated with PP/AgNPs masterbatch in batch experiment. The concentrations of nanoparticles in masterbatch were varied to be 200, 500, 1000, 1200 and 1500 ppm. At 1500 ppm, the compromised antibacterial activities were observed in all tested bacterial species with 2-h-long exposure time. The results in this study could be further applied to fit in operation of molasse use in industrial scale in various application, including bioethanol production.

scholarly journals Synthesis and Comparative Biological Properties of Ag-PEG Nanoparticles with Tunable Morphologies from Janus to Multi-Core Shell Structure

Materials ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 1787 ◽  
Author(s):  
Mengda Xu ◽  
Jie Liu ◽  
Xiankui Xu ◽  
Shanhu Liu ◽  
František Peterka ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Silver Nitrate ◽  
Shell Structure ◽  
Irradiation Time ◽  
Antibacterial Activities ◽  
Biological Properties ◽  
Hybrid Nanoparticles ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Core Shell Structure

Silver nanoparticles synthesized with polymers as coating agents is an effective method to overcome their poor stability and aggregation in solution. Silver-polyethylene glycol (Ag-PEG) nanoparticles were synthesized with the thiol-functionalized polyethylene glycol (SH-PEA) as the coating, reducing and stabilizing agent. The UV irradiation time, polymer and silver nitrate concentration for the synthesis were investigated. The concentration of silver nitrate had significant effect on the morphology of Ag-PEG nanoparticles. When increasing the concentration of silver nitrate, SEM and TEM images showed that Ag-PEG nanoparticles changed from Janus to multi-core shell structure. Meanwhile, pure silver particles in the two hybrid nanoparticles presented spherical shape and had the similar size of 15 nm. The antibacterial activities and cytotoxicity of the two structural Ag-PEG nanoparticles were investigated to understand colloid morphology effect on the properties of AgNPs. The results of antibacterial activities showed that the two structural Ag-PEG nanoparticles exhibited strong antibacterial activities against Staphylococcus aureus, Escherichia coli and Bacillus subtilis. The Janus nanoparticles had larger minimal inhibitory concentration (MIC) and minimum bacterial concentration (MBC) values than the multi-core shell counterparts. The results of cytotoxicity showed the Janus Ag-PEG nanoparticles had lower toxicity than the multi-core shell nanoparticles.

scholarly journals Studies of Mixed Ligand Lanthanum Complexes: Synthesis, Spectral Interpretations and Antibacterial Study

2021 ◽  
pp. 161-174
Author(s):  
Sunil Patil ◽  
Bhushan Langi ◽  
Minakshi Gurav ◽  
Digambar Patil
Keyword(s):  
Magnetic Susceptibility ◽  
Ftir Spectroscopy ◽  
Elemental Analysis ◽  
Metal Ion ◽  
Antibacterial Activities ◽  
Decomposition Temperature ◽  
Mixed Ligand ◽  
Visible Spectroscopy ◽  
Uv Visible ◽  
Xrd Techniques

The mixed ligand lanthanum complexes have been synthesized using polydentate ligands. The (2Z)-2-(N-hydroxyimino)-1,2-diphenylethan-1-ol (cupron) was used as a primary ligand and L-isolucine, L-threonine, L-lysine and L-glycine were used as secondary ligands for the synthesis. Conductometry, elemental analysis, magnetic susceptibility measurements, complexometric estimation, UV-Visible spectroscopy, FTIR spectroscopy, thermal analysis and XRD methods were used for structural interpretation of all synthesized complexes. All complexes were solid and white and slight yellow in colour. They are non-electrolytic and diamagnetic in nature, as confirmed by conductometric and magnetic susceptibility methods respectively. All the complexes were synthesized by combination of lanthanum metal ion, primary ligand and secondary ligands in 1:2:1 ratio which was confirmed by elemental analysis. During reaction between lanthanum ion, primary ligand and secondary ligands there was transition of electrons which was confirmed by UV-Visible spectroscopy. Identification of functional group in the complexes was carried out and confirmed by FTIR spectroscopy. The decomposition temperature and thermal behaviour of the complexes have been concluded by thermal and XRD techniques. Tube dilution and agar cup methods have been employed to study antibacterial activities of all synthesized lanthanum complexes. The inhibition potential was seen to some extent.  

scholarly journals AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI DARI DERIVAT METIL EKSTRAK ETANOL DAUN GAMBIR (Uncaria gambir)

2020 ◽  
Vol 15 (4) ◽  
pp. 145
Author(s):  
IRMA KRESNAWATY ◽  
ACHMAD ZAINUDDIN
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Chemistry Laboratory ◽  
Bacteria Growth ◽  
Ft Ir ◽  
Uv Visible ◽  
Chloroform Methanol

<p>ABSTRAK</p><p>Banyak tanaman yang dilaporkan memiliki kandungan senyawabahan aktif antioksidan dan antibakteri. Salah satu tanaman Indonesia yangmemiliki aktivitas ini adalah gambir (Uncaria gambir). Pada penelitian ini,ekstrak etanol daun gambir diubah menjadi derivat metilnya untukmembuatnya lebih larut dalam lemak dan diamati pengaruh derivatisasitersebut terhadap aktivitas antioksidan di laboratorium kimia organik danpengujian aktivitas antibakteri di laboratorium mikrobiologi UniversitasPadjadjaran. Penelitian ini dilaksanakan di laboratorium Kimia OrganikJurusan Kimia dan laboratorium Mikrobiologi Jurusan Biologi UniversitasPadjadjaran dari bulan Desember 2004 - Juli 2005. Ekstrak gambirdimetilasi  menggunakan  dimetil  sulfat  (DMS)  dan  dimurnikanmenggunakan kromatografi kolom dengan pelarut bergradien (kloroform :metanol = 99:1 ; 98:2 ; 95:5 ; 80:20 ; 70:30; dan 50:50 v/v) dan kemudianmenggunakan kloroform : metanol = 99 : 1 v/v. Aktivitas antioksidanmenunjukkan penurunan yang tampak dari peningkatan, yaitu : IC 50  13,41ppm untuk ekstrak etanol menjadi 121,81 ppm untuk hasil metilasi.Aktivitas antibakteri juga menunjukkan penurunan setelah dimetilasikarena adanya penurunan diameter hambat pertumbuhan bakteri. Duaisolat (isolat 1 dan 2) yang diperoleh dari hasil pemurnian dikarakterisasimenggunakan spektrofotometer UV-Visible dan FT-IR. Hasil yangdiperoleh mengindikasikan adanya senyawa fenolik yang ditunjukkan olehregang –OH pada 3445 dan 3448 cm -1 dan regang CH aromatik pada 3010dan 3030 cm -1 . Isolat 1 memiliki aktivitas antioksidan dan antibakteri yanglebih tinggi dibandingkan isolat 2.</p><p>Kata kunci : Uncaria gambir, derivat metil, antibakteri dan aktivitasantioksidan</p><p>ABSTRACT</p><p>The antioxidant and antibacterial activities of ethanolextract of gambir leaves (Uncaria gambir)</p><p>There are many plants in Indonesia reported to contain antioxidantand antibacterial substances. One of them having these activities is gambir(Uncaria gambir). In this research, ethanol extract of gambir leaves waschanged into its methyl derivate in order to make it more soluble in fats.The effect of the derivatization on antioxidant activity was observed atorganic chemistry laboratory and antibacterial activity was observed atmicrobiology laboratory of the Padjadjaran University. This research wascarried out in December 2004 to July 2005. Gambir extract wasmethylized using dimethylsulphate (DMS) and then purified usingcoloumn chromatography with gradient solvents (chloroform : methanol =99:1; 98:2; 50:50; 80:20; 70:30; and 50:50 v/v), and then with chloroform :methanol = 99:1 v/v. The antioxidant activity showed a decrease indicatedby an increase of IC 50 from 13.41 ppm for ethanolic extract to become121.81 ppm for the methylated compounds. The antibacterial activity alsoshowed a decrease after methylization due to the decrease of inhibitiondiameter of bacteria growth. Two isolates (isolate 1 and 2) obtained fromthe  purification  steps  were  characterized  using  UV-Visiblespectrophotometer and FT-IR. The results indicated the existence ofphenolic compunds showed by -OH stretching in 3,445 and 3,448 cm -1 ;and CH aromatic stretching in 3,010 and 3,030 cm -1 . Isolate 1 was higherin antioxidant and antibacterial activities than isolate 2.</p><p>Key words : Uncaria gambir, methyl derivative, antibacterial, antioxidantactivities</p>

scholarly journals Synthesis, Characterization and Antimicrobial Evaluation of Cobalt(III) Complexes of 4-(2-Substituted Phenylimino)-2-(4-Substituted Phenyl)-4H-chromen-3-ol

2019 ◽  
Vol 31 (9) ◽  
pp. 2015-2021
Author(s):  
Ashok K. Singh ◽  
Suresh K. Patel ◽  
Asif Jafri
Keyword(s):  
Magnetic Measurements ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Mass Spectral ◽  
Antimicrobial Evaluation ◽  
Uv Visible ◽  
Paper Disc

A series of eight Co(III) complexes [CoL1-8(H2O)2Cl] (I-1 to I-8) incorporating 4-(2-substituted phenylimino)-2-(4-substituted phenyl)-4H-chromen-3-ol, as a tridentate imino flavone ligands (L1 to L8, 2-sub. = NH2, SH, 4-sub. = OMe, OH, Cl, NMe2) have been synthesized, characterized and the geometry of the complexes were optimized by DFT. The chemical structure of synthesized imino flavone ligands and their complexes were characterized by elemental analysis, 1H NMR, 13C NMR, UV-visible, IR, ESI-mass spectral data, conductometric and magnetic measurements. The synthesized compounds have been screened for their in vitro antibacterial activities against bacteria Vibrio cholerae, Salmonella typhi, Staphylococcus aureus, Escherichia coli and antifungal activities against fungi Candida albicans and Aspergillus flavus by paper disc diffusion method. The complexes I-3, I-4, I-7 and I-8 showed good antimicrobial activities against pathogens.

scholarly journals Metal-Based Scaffolds of Schiff Bases Derived from Naproxen: Synthesis, Antibacterial Activities, and Molecular Docking Studies

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1237 ◽  
Author(s):  
Muhammad Shaheen ◽  
Shanshan Feng ◽  
Mehwish Anthony ◽  
Muhammad Tahir ◽  
Mubashir Hassan ◽  
...  
Keyword(s):  
Schiff Base ◽  
Metal Complexes ◽  
Schiff Base Ligand ◽  
Antibacterial Activities ◽  
Docking Studies ◽  
Computational Docking ◽  
Square Planar ◽  
Uv Visible ◽  
Xrd Techniques ◽  
Bioactivity Profile

We report here the synthesis, characterization, and antibacterial evaluation of transition metal complexes of Ni, Cu, Co, Mn, Zn, and Cd (6a–f), using a Schiff base ligand (5) derived from naproxen (an anti-inflammatory drug) and 5-bromosalicylaldehyde by a series of reactions. The ligand and the synthesized complexes were characterized by elemental analysis, UV-Visible, FTIR, and XRD techniques. The ligand 5 behaves as a bidentate donor and coordinates with metals in square planar or tetrahedral fashion. In order to evaluate its bioactivity profile, we screened the Schiff base ligand and its metal complexes (6a–f) against different species of bacteria and the complexes were found to exhibit significant antibacterial activity. The complexes showed more potency against Bacillus subtilis as compared to the other species. Moreover, we modeled these complexes’ binding affinity against COX1 protein using computational docking.

Nanomaterials for Deep Tumor Treatment.

2020 ◽  
Vol 20 ◽  
Author(s):  
Daria Yu. Kirsanova ◽  
Zaira M. Gadzhimagomedova ◽  
Aleksey Yu. Maksimov ◽  
Alexander V. Soldatov
Keyword(s):  
Visible Light ◽  
Tumor Treatment ◽  
Internal Organs ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
New Methods ◽  
In Vivo Experiments ◽  
Uv Visible

: According to statistics, cancer is the second leading cause of death in the world. Thus, it is important to try to solve this medical and social problem by developing new methods for cancer treatment. An alternative to more wellknown approaches, such as radiotherapy and chemotherapy, is photodynamic therapy (PDT) which is limited to the shallow tissue penetration (< 1 cm) of visible light. Since the PDT process can be initiated in deep tissues by X-ray irradiation (X-ray induced PDT, or XPDT), it has a great potential to treat tumors in internal organs. The article discusses the principles of therapies. The main focus being on various nanoparticles used with or without photosensitizers, which allow the conversion of X-ray irradiation into UV-visible light. Much attention is given to the synthesis of nanoparticles and analysis of their characteristics such as size and spectral features. The results of in vitro and in vivo experiments are also discussed.

Biogenic Gold Nanoparticles and its Antibacterial Activities: Houttuynia Cordata Leaf Extract

2014 ◽  
Vol 1051 ◽  
pp. 392-397
Author(s):  
T.V.M. Sreekanth ◽  
In Yong Eom
Keyword(s):  
Gold Nanoparticles ◽  
Leaf Extract ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Houttuynia Cordata ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Visible Spectra ◽  
Uv Visible

Gold nanoparticles (AuNPs) can be prepared in a number of chemical techniques, which are not environmentally friendly. Biosynthesis of nanoparticles by plant extracts is currently under exploitation. In this work, we describe an eco-friendly technique for green synthesis of AuNPs from AuCl4 solution using the Houttuynia cordata leaf extract as reducing agent. The AuNPs were characterized using UV-Visible spectroscopy, SEM, TEM, FTIR and AFM. The UV-Visible spectra indicate a strong plasma resonance that is located at 535 nm. The antibacterial activity of AuNPs was performed on various gram positive and gram negative bacteria. The AuNPs showed more inhibitory activity on gram negative than gram positive bacteria.

scholarly journals Green Synthesis and Antibacterial Activity of Cobalt Nanoparticle from Calotropis gigantae

2020 ◽  
Vol 64 (2) ◽  
Author(s):  
Sauban Ghani ◽  
Farheen Ghani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activities ◽  
Indirect Measurement ◽  
Cobalt Nanoparticles ◽  
Wild Plant ◽  
Infrared Spectrometry ◽  
Calotropis Gigantea ◽  
Uv Visible ◽  
Average Size

Abstract. Cobalt nanoparticles (CoNPs) were successfully synthesized by taking a simple green synthetic route using metal salts and flower extracts of a wild plant Calotropis gigantea which act as reducing as well as the stabilizing agent. The synthesized Cobalt nanoparticles (CoNPs) were characterized using various techniques such as UV-visible spectrophotometry and Fourier transform infrared spectrometry. Size characterization of the samples was made by Dynamic light-scattering (DLS) and Transmission electron microscope (TEM). The micrographs of the synthesized CoNPs showed the formation of spherical nanoparticles with an average size of 13 ± 5 nm. The CoNPs were stable at room temperature (25 °C) for six months. Zeta potential values provided an indirect measurement of the net charge on the Cobalt nanoparticles (CoNPs) surface. The antibacterial activities of cobalt nanoparticles (CoNPs) were studied under varying concentrations of CoNPs with respect to Staphylococcus aureus and Escherichia coli.                                             Resumen. Las nanopartículas de cobalto (CoNPs) se sintetizaron con éxito al tomar una ruta sintética verde simple usando sales metálicas y extractos de flores de una planta silvestre Calotropis gigantea que actúa como agente reductor y estabilizador. Las nanopartículas de cobalto sintetizadas (CoNPs) se caracterizaron utilizando diversas técnicas, como la espectrofotometría UV-visible y la espectrometría infrarroja por transformada de Fourier. La caracterización del tamaño de las muestras se realizó mediante dispersión dinámica de luz (DLS) y microscopio electrónico de transmisión (TEM). Las micrografías de los CoNPs sintetizados mostraron la formación de nanopartículas esféricas con un tamaño promedio de 13 ± 5 nm. Los CoNPs fueron estables a temperatura ambiente (25 °C) durante seis meses. Los valores potenciales de Zeta proporcionaron una medición indirecta de la carga neta en la superficie de nanopartículas de cobalto (CoNPs). Las actividades antibacterianas de las nanopartículas de cobalto (CoNPs) se estudiaron bajo concentraciones variables de CoNPs con respecto a Staphylococcus aureus y Escherichia coli.

scholarly journals Efficient synthesis of plant-mediated silver nanoparticles and their screening for antimicrobial activity

2017 ◽  
Vol 4 (3) ◽  
pp. 143-150 ◽  
Author(s):  
Kamlesh Shukla ◽  
Bhoopander Giri ◽  
Rashmi Dwivede
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Color Change ◽  
Bacterial Species ◽  
Nitrate Solution ◽  
Reducing Agent ◽  
Curvularia Lunata ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy

Now days, the development of safe, cost effective, reliable and eco-friendly processes for the synthesis of nanoparticles is an important aspect of nanotechnology. Among the various agents, plants show immense potential for the synthesis of nanoparticles. The bio-molecules found in plants induce reduction of Ag+ ions from silver nitrate to silver nanoparticles (AgNPs); therefore, in the present work, the aqueous leaves extract of the plant was used as reducing agent for the synthesis of silver nanoparticles. We synthesized extracellular silver nanoparticles using extract of the leaves of four different medicinal plants which act as a reducing agent at room temperature. The characteristic color change was observed on addition of plant extract to the silver nitrate solution due to their specific properties (Surface Plasmon Resonance). UV-Vis spectroscopy was used for the characterization of the silver nanoparticles. Green synthesized nanoparticles are evaluated for their antimicrobial activity against the Gram-positive and Gram-negative bacteria as well as two pathogenic fungi Aspergillus fumigatus and Curvularia lunata. The silver nanoparticles (SNPs) of selected plant parts have shown more toxicity towards bacterial species than that of the fungal species. Comparing with simple plant extracts, the SNPs exhibited greater antimicrobial efficacy and advantage over conventional antibiotics to which these microorganisms usually impart resistance.

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