Influence of early spring feeding on the development of bee families in the natural lands of Polissya

To ensure intensive early spring development of bee colonies in the natural lands of Polissya Zhytomyr region in the winter-spring period it is necessary to feed stimulating food. Therefore, the aim of the work is to study the influence of spring feeding with sugar-honey dough on the development and honey productivity of bee families of Ukrainian breed in conditions of clean and radioactively contaminated natural lands of Zhytomyr Polissya. The influence of feeding bee colonies with sugar-honey dough (powdered sugar, centrifuged honey, water) using pure and radioactively contaminated honey on the intensity of development and honey productivity was studied. To do this, two groups were created with experimental families of Ukrainian bee breeds, selected on the principle of analogs, one of which was in a radioactively clean area, and the other in radioactively contaminated soils 137 Cs within 15 Ki/km² and above. A comparative assessment of the early spring development of families was made: from March 16 to May 20, with an interval of 21 days, the number of open and sealed brood, egg production of queens during the honey harvest season was determined. Honey productivity at the honey harvest from natural lands was determined by the amount of honey obtained in spring and summer. Early spring feeding with sugar-honey dough promotes intensive development of families, and the content of radioactively contaminated honey does not have a negative impact on their development. After the end of the season of honey collection from natural lands, bee families of Ukrainian breed, both in the radioactively contaminated and clean zone, had a medium strength and could successfully overwinter.

COVID-19 or not COVID-19, that is the question. The role of screening criteria and point-of-care lung ultrasound in the triage decision-making process in an Emergency Department during the phase 2 of the COVID-19 Italian epidemic

In the COVID-19 era the real challenge for the Emergency Departments (ED) is to avoid the spread of the viral infection within the so called “clean area” of the emergency room and the hospital. Different protocols have been proposed and adopted in the EDs to quickly identify suspected COVID-19 patients and to correctly manage these patients, all based on clinical and epidemiological criteria. To the best of our knowledge, our pre-triage decisional making-process first integrates the pre-triage interview with pointof- care Lung Ultrasound (LUS) performed in the triage area. The aim of our study is to assess the sensitivity and specificity of our screening clinical and/or epidemiological criteria, and to investigate the role of LUS in the triage decision-making process during the “phase 2” of the COVID-19 Italian epidemic. Our study confirms the pivotal role of the triage in the decision-making process and the management of the entire ED, and it demonstrates that further studies are necessary to validate the role of LUS as tool to promptly identify COVID-19 patients, if combined with a correct pre-triage interview.

Scots pine in different ecological conditions in the south of the Central Black Earth region: features of the genetic structure of the glutamate dehydrogenase locus

A comparative analysis of two Scots pine plantations growing in different ecological conditions of the steppe zone in the south of the Central Black Earth region (Kantemirovsky District of the Voronezh Region) has been carried out. One plantation is located in a relatively ecologically clean area, outside the urban environment, the other one - within the village Kantemirovka. The enzyme system has been studied; it is an important link in the main metabolic pathways in plant organisms influencing the general condition of plants. In the studied stands the genetic structure of the glutamate dehydrogenase locus has been analyzed, one of the alleles of which ( Gdh-1 ) in Scots pine is an embryonic semi-metal. It has been found that in a weakened plantation located in an urban environment and exposed to increased anthropogenic impact, the proportion of this allele and the proportion of genotypes homozygous for it is significantly (p 0,01) higher than in the population from an ecologically clean zone. It is known that organisms with semi-lethal genes in their genotype possess a complex of compensatory genes, which determines their increased resistance to unfavorable factors. An increase in the proportion of semi-lethal alleles in a plantation growing under anthropogenic load can be an adaptive mechanism for increasing resistance at the population level to stress effects of different nature.

KEMAMPUAN BAKTERI ASAM LAKTAT DALAM MENGHAMBAT Salmonella thypii

Lactic acid bacteria are known to have the ability to produce antimicrobial compounds known as bacteriocins. This study aims to determine the ability of lactic acid bacteria to inhibit the growth of Salmonella thypii bacteria. In this study, the lactic acid bacteria used were a collection of the Biology Laboratory of the University of Medan Area. Eight types of lactic acid bacterial isolates were used, including IN01, IN02, SF01, SF02, NN01, NN02 SPU01 and SPU04. A confirmation test was carried out on the growth of lactic acid bacteria and Salmonella thypii by visual and microcopic confirmation through differential staining. The results showed that all lactic acid bacteria were a group of gram-positive bacteria in the form of bacilli. The survival of lactic acid bacterial isolates in the environment with variations in pH and salt levels was indicated by the number of colonies that grew after the incubation period. The antagonistic ability of lactic acid bacteria in suppressing pathogenic bacteria was proven by the presence of an inhibition zone in the form of a clean area around the pathogenic colony. It is known that the SPU01 and SF01 bacterial isolates have the highest inhibition zone values, namely 29.79 mm and 28.13 mm.

Identification of the Source for Salmonella Contamination of Carcasses in a Large Pig Slaughterhouse

To identify the major source of Salmonella contamination in a pig slaughterhouse, samples were collected from the clean and unclean area and Salmonella isolates were further typed. Carcasses entering the clean area showed a Salmonella contamination rate of 96.7% in the oral cavity and 55.0% in the rectum content samples. Evisceration seemed not to be critical as the contamination rate of the carcasses was similar before (16.7%) and after (18.3%) this slaughter step. In the unclean area, a limited number of oral cavity samples were positive after bleeding, while a dramatic increase of positives was observed after dehairing. Salmonella was detected in up to 0.01 mL of the recycled water collected from the dehairing machine. Genotyping of Salmonella isolates showed that similar pulsotypes were present in the oral cavity and recycled water. Based on these observations it can be concluded that the recycled water used in the dehairing machine was the major source for the carcass contamination in this slaughterhouse.

CLASSIFICATION OF RECREATIONAL LOCATIONS OF RURAL GREEN TOURISM IN ODESA REGION

The article highlights the importance of the role of rural green tourism in restarting the Ukrainian economy, given that the inertia of the surge in domestic tourism caused by the coronavirus COVID-19 pandemic will work in the coming years. The importance of recreational activities in the field of rural green tourism is considered. The problems of the conceptual and terminological description of the infrastructure of the recreational industry are outlined. Expansion of the nomenclature of key terms in the field of rural green tourism in the context of highlighting the point elements of the territorial structure is proposed. The concept of “recreational location of rural green tourism” has been introduced for use in the practice of the recreational industry in order to strengthen the territorial aspects of the research of recreational activities. The content of the concept “recreational location of rural green tourism” is characterized as a point element of the territorial infrastructure in the field of rural tourism in the region, which combines the territorial localization of the place of receiving tourist services (rural ecologically clean area), accommodation facility (local object) and indicates the special nature of the activity (recreation). It is indicated that, according to the taxonomic criterion, it is a micro-site, a place, and according to the typological criterion, it is a recreational and tourist object with the functions of accommodation and provision of recreational services. The components of the recreational potential of the Odesa region, which have the necessary properties for the successful functioning of a competitive tourist product in the regional and national tourist markets, have been analyzed. The importance of the classification of recreational locations of rural green tourism in the Odessa region for further systematization of information for the purpose of convenient practical use by specialists from various industries and consumers of recreational services has been substantiated. The classification of the locations of rural green tourism in the Odessa region according to three criteria has been proposed and five types of recreational locations have been identified.

The Impact of Radiation pollution of Environment on students’ physical Development and health

Aim: To study the impact of small doses of radiation pollution of environment on the physical development and health of students from different areas of residence. materials and methods: The study was conducted at Polissya National University (Zhytomyr, Ukraine) during 2012-2020. 647 students of different specialties in the age of 17-23 were interviewed about the use of preventive measures to eliminate the consequences of the Chernobyl Nuclear Power Plant (CNPP) accident in 1986 and other factors of radiation pollution of environment. In addition, 127 students who were born, lived for some time or periodically lived in the area of low doses of radioactive contamination (58 males and 69 females) and 238 students who lived in the clean area from radioactive contamination (113 males and 125 females) were interviewed to compare the samples. Results: The comparative analysis of the students’ physical development from different areas of residence showed that the students from the clean area have better indicators of average body height, body weight, Erisman index with no significant differences (P>0.05). Significant differences were revealed in the indicators of the circumference of the chest and the dynamometry of the hand (P<0.05-0.01). Conclusions: It was established that long-term residence in the area with low doses of radioactive contamination has a negative impact on the physical development and health of the students. The students who have lived for a long time in the area of radioactive contamination more often suffer from a disease, miss classes and get tired faster during physical education sessions. It was clarified that the majority of the students are not involved in health preservation activities, but only in cases when they face significant health problems associated with the need to see a doctor, disability, etc.

TRAVELLERS' PERCEPTION OF WORSHIP FACILITIES FOR MULTIMODAL USERS OF MRT SBK LINE

The complete opening of the new Mass Rapid Transit System (MRT) Sungai Buloh - Kajang (SBK) of Klang Valley, Malaysia since July 2017 has been supported positively by transit researchers and public transport advocators alike. Multimodal public transport users make trips, usually involving transferring at interchanges. These interchanges are provided with several amenities facilitating each trip leg. This research recorded the perception of users of worship facilities provided at an interchanging node where several rails, bus, taxi, and other paratransit systems meet. This new rail system intersects with the other systems at Muzium Negara station. Using intercept off-board face to face questionnaire survey method, perception of the quality of worship facilities was captured between May and August 2019. Several explanatory variables such as socio economic and trip characteristics were set against the perception to identify factors influencing the perception. Results of the correlational analysis showed that both socio-demographic and trip characteristics influenced perception to various statistical significance degrees. Findings suggested that worship facilities were most utilised by passengers of MRT who relatively lower income, engaged in the public, specifically educational sector. Usage of MRT for commuting within the Klang Valley on monthly basis. The improvements in ventilation systems, prayer hall size and design, floor cleanliness, lighting and mirror design as well as shoe rack/clean area design were most sought by these passengers. As such, these should be the focus of strategies to be promptly adopted by interchanging nodes building managers to increase the quality of worship places provision.