Valorization of waste bark for biorefineries: chemical characterization of Eucalyptus camaldulensis inner and outer barks

Bark wastes today are viewed as a high-value resource for biorefinery due to their chemical richness and diversity. This work presents a comprehensive chemical characterization of the inner bark and the outer bark of Eucalyptus camaldulensis cultivated in Algeria. The extractives were first isolated with an Accelerated Solvent Extractor (ASE) and then analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The content of pre-extracted bark in cellulosic polysaccharide and free sugar monomers was determined by Gas Chromatography (GC). The hemicellulose composition and amount was determined after the acid methanolysis and GC. The amount of lignin was determined gravimetrically by the Klason lignin method and the acid soluble lignin was determined by the UV method. Formic and acetic acids in the bark were determined by HPLC after alkaline hydrolysis. It was found that the extractives content were similar in the outer bark (0.85%) and the inner bark (0.88%). The cellulose content was higher in the outer bark (33.4%) than in the inner bark (28.7%). Lignin and the total hemicellulose contents were more abundant in the outer bark (31.7, 26.2%) than in the inner bark (28.6, 19.3%) whereas, sugar monomers were more abundant in the inner bark (4.4%) than in the outer bark (3.8%). The variation in acetic and formic acids and ash contents between the outer bark (1.5, 0.006 and 2.5%) and the inner bark (1.3, 0.005 and 2.4%) was small. The obtained results showed that the bark can be considered a suitable feedstock for lignocellulosic biorefinery and also for the extraction of bioactive compounds that can be used in different sectors.

Novel Intact Polar and Core Lipid Compositions in the Pyrococcus Model Species, P. furiosus and P. yayanosii, Reveal the Largest Lipid Diversity Amongst Thermococcales

Elucidating the lipidome of Archaea is essential to understand their tolerance to extreme environmental conditions. Previous characterizations of the lipid composition of Pyrococcus species, a model genus of hyperthermophilic archaea belonging to the Thermococcales order, led to conflicting results, which hindered the comprehension of their membrane structure and the putative adaptive role of their lipids. In an effort to clarify the lipid composition data of the Pyrococcus genus, we thoroughly investigated the distribution of both the core lipids (CL) and intact polar lipids (IPL) of the model Pyrococcus furiosus and, for the first time, of Pyrococcus yayanosii, the sole obligate piezophilic hyperthermophilic archaeon known to date. We showed a low diversity of IPL in the lipid extract of P. furiosus, which nonetheless allowed the first report of phosphatidyl inositol-based glycerol mono- and trialkyl glycerol tetraethers. With up to 13 different CL structures identified, the acid methanolysis of Pyrococcus furiosus revealed an unprecedented CL diversity and showed strong discrepancies with the IPL compositions reported here and in previous studies. By contrast, P. yayanosii displayed fewer CL structures but a much wider variety of polar heads. Our results showed severe inconsistencies between IPL and CL relative abundances. Such differences highlight the diversity and complexity of the Pyrococcus plasma membrane composition and demonstrate that a large part of its lipids remains uncharacterized. Reassessing the lipid composition of model archaea should lead to a better understanding of the structural diversity of their lipidome and of their physiological and adaptive functions.

TECHNOLOGICAL METHODS OF CASCADE BIO CONVERSION OF SUNFLOWER HUSK

В работе рассматриваются возможности использования подсолнечной лузги в качестве субстрата для культивирования мицелиальных маточных культур вешенки устричной (Pleurotusostreatus) и шампиньона двуспорового. Проведена сравнительная оценка утилиза­ции соломистого субстратамицелием грибов. Изучены особенности развития мицелия и плодовых тел вешенки устричной на субстратах из лузги подсолнечника, дериватизирован­ных путем кислотного метанолиза. Определены возможности повышения урожайности ве­шенки устричной на субстратах, подвергнутых дериватизации. Показаны возможности ис­пользования отработанных субстратов после культивирования вешенки, в качестве биоком­поста, вносимого под культуру огурцов сорта «Кузнечик F1» в условиях открытого грунта. The paper discusses the possibilities of using sunflower husk as a substrate for the cultivation of mycelial stock cultures of oyster mushroom (Pleurotusostreatus) and garden mushroom. A comparative assessment of the utilization of a straw substrate by fungal mycelium was carried out. The features of the development of oyster mushroom mycelium and fruit bodies on substrates from sunflower husks derivatized by acid methanolysis were studied. The possibilities of increasing the yield of oyster mushrooms on substrates subjected to derivatization were determined. The possibilities of using the used substrates after the cultivation of oyster mushroom as a biocompost introduced into cucumbers of the Kuznechik F1 variety in open ground are shown.

Methodology optimization for the analysis of phenolic compounds in chestnut (Castanea sativa Mill.)

Phenols are bioactive substances of great interest because of their involvement in plant physiology, their use in many industrial processes, and their impact on human health. This work aims to summarize the varied approaches to the phenolic analysis of chestnut (bark and wood of trunk and branches, leaves, catkins, burs, and fruit) and to collate the optimal conditions into an easy to follow and execute protocol. Phenolic compounds were extracted by solid–liquid extraction and separated by liquid–liquid extraction. Total phenols content was determined by Folin–Ciocalteu assay, condensed tannins by vanillin assay, and hydrolyzable tannins (gallotannins and ellagitannins) by high-performance liquid chromatography quantification of methyl gallate and ellagic acid following acid methanolysis. The lowest temperature for conservation (–80 ℃), lyophilization, and milling (liquid N2) were the most effective pretreatments for samples. For quantification of tannins, the use of water clearly reduced the sensitivity of the analysis of condensed tannins, whilst the more efficient degradation capacity of sulfuric acid improved the methanolysis of hydrolyzable tannins. These findings were validated using a range of chestnut tissues, and thus confirm the utility and effectiveness of this easy to implement, cost-effective, and efficient protocol.

Xylan accessibility of bleached eucalypt pulp in alkaline solutions

The accessibility of glucuronoxylan from a refined, bleached Eucalyptus globulus industrial kraft pulp in 0–18% aqueous NaOH solution at room temperature (25°C) was studied. The extraction profile revealed a maximum extraction of xylan in the pulp at about 10–12% NaOH concentration and was explained by the maximum swelling of the pulp according to the Gibbs-Donnan equilibrium. The kinetics of xylan removal and the monitoring of its structural features were performed at 5 and 10% NaOH concentrations. The maximum yields of xylans with 10% and 5% NaOH were as high as 90% and 60% for 2 h extraction, respectively. The structural features of xylan were assessed by acid methanolysis and one-dimensional (1D)/two-dimensional (2D) nuclear magnetic resonance (NMR), and the molecular weight by size exclusion chromatography (SEC). The xylan extracted with 10% NaOH had a slightly higher molecular weight and a lower branching with uronic moieties than the xylan extracted with 5% NaOH. The former was less pure (contained more β-cellulose) than the latter. Structural studies by NMR resulted in the conclusion that there are at least two types of xylans removed from the pulp: one xylan with relatively high 4-O-methyl-α-D-glucuronosyl [MeGlcA-(1→] and [→2)-MeGlcA-(1→] substituents and another xylan with a much lower substitution with uronic residues.

Biodiesel Production by Acid Methanolysis of Slaughterhouse Sludge Cake

Biosludge is a normal form of accumulating microbial populations inside the sewage or wastewater treatment facilities. Excessive sludge in the wastewater treatment basins has to be removed periodically to ensure good water quality of the effluent. This study aims to evaluate the feasibility of biodiesel production by transesterification of slaughterhouse sludge cake. The sludge cake was collected from a selected commercial slaughterhouse and transesterified with methanol, n-hexane, and acids (e.g., sulfuric acid or hydrochloric acid) at 55 °C. Three acid concentrations (2%, 4%, and 8%, v/v) in methanol under four reaction time periods (4, 8, 16, and 24 h) were applied. Results showed that the highest accumulated fatty acid methyl ester (FAME) yields of 2.51 ± 0.08% and 2.27 ± 0.09% were achieved when 8% (v/v) of H2SO4 or HCl were added in a 4 h reaction time, respectively. Methyl esters of palmitic acid (C16:0), palmitoleic acid (C16:1), stearic acid (C18:0), and oleic acid (C18:1n9c) were the major components of biodiesel from acid methanolysis of slaughterhouse sludge cake. Experimental and analytical results of acid methanolysis of slaughterhouse sludge cake showed that acid methanolysis of sludge cake was one of the feasible and practical options to recycle sludge waste and produce renewable energy.

Acid methanolysis: An analytical tool for estimating the proportions of different pulp types in deinked pulp or recovered paper

A method was developed to estimate the composition of deinked pulp or recovered paper in termsof hardwood kraft pulp, softwood kraft pulp, and mechanical pulp through analysis of the sugar units in hemicellulosesand pectins. Different pulps (i.e., bleached chemical pulp from hardwoods and softwoods and mechanical pulp) were obtainednfrom different mills. The pulps were mixed in different proportions and dried. The content of arabinose, xylose, mannose, galactose, rhamnose, galacturonic acid, and 4-O-methyl-glucuronic acid, sugar units in hemicelluloses and pectins in the different pulps and in the mixtures were determined by acid methanolysis and gas chromatography. The contents of the sugar units were significantly different in the three pulp types but quite similar in the same pulp type, irrespectively of bleaching sequence. The modeling was performed using standard partial least squares regression (PLSR) and cross-validation using venetian blinds subsets. The data were autoscaled before regressions. The best model had two latent variables. The cumulative variation of X captured by the model was 0.988 (R2X), the cumulative variation of Y captured by the model was also 0.988 (R2Y), and the cumulative cross-validated variation of Y captured by the model was 0.974 (Q2). Accordingly, the PLS model is more than capable of predicting the validation set. It was concluded that the proportion of chemical pulp from hardwoods, chemical pulp from softwoods, and mechanical pulp can be estimated in recovered paper or deinked pulp using a mathematical model based on the content of selected sugar units in hemicelluloses and pectins.

Extraction of galactoglucomannan from spruce wood with pressurised hot water

Sapwood chips and ground wood of spruce have been extracted with pressurised pure water in an accelerated solvent extractor at temperatures from 100 to 180°C. The water extracts were freeze-dried, weighed, and analysed for carbohydrates by acid methanolysis followed by GC determination of monomeric sugars. Lignin was determined by measuring UV absorption and acetyl groups by HPLC after alkaline hydrolysis. Average molar masses of hemicelluloses in water extracts were determined by HPSEC-MALLS after filtration. Typically, approximately 70% of the total weighed extracts were composed of carbohydrates derived from hemicelluloses. Approximately 75% of the extracted carbohydrates were from galactoglucomannan (GGM). Other extracted substances were xylans, arabinogalactans, lignin and acetic acid. As much as 80–90% of the GGM in the wood, i.e., approximately 15% based on the original wood, was extracted from ground wood at 170–180°C for an extraction time of 1 h. The yields from chips were lower: approximately 60% from that of ground wood. The hemicelluloses were partly hydrolysed during the extractions at 160–180°C. Xylans and arabinogalactans were hydrolysed to a higher extent than GGM. Acetyl groups in GGM were also partly hydrolysed, which resulted in lower GGM solubility. A key factor for achieving a high yield of high-molar mass GGM is an optimised pH profile in order to minimise hydrolysis of acetyl groups and hydrolytic cleavage of GGM chains.