Time Mating Guinea Pigs by Monitoring Changes to the Vaginal Membrane throughout the Estrus Cycle and with Ultrasound Confirmation

One of the greatest challenges to the development and implementation of pregnancy therapeutics is the ability to rigorously test treatments in clinically relevant animal models. Guinea pigs offer a unique advantage in studying the placenta, fetal development, and reproductive health as they have similar developmental milestones to humans, both throughout gestation and following birth. Tracking the guinea pig estrus cycle is imperative to ensuring appropriately timed mating and can be performed by monitoring the guinea pig vaginal membrane. Here, we describe a methodology to efficiently and accurately time mate guinea pigs, and provide a picture representation of changes to the guinea pig vaginal membrane throughout the estrus cycle. Utilization of this monitoring enabled a 100% pregnancy success rate on the first mating attempt in a cohort of five guinea pigs. This approach, along with early pregnancy ultrasounds as a secondary method to confirm pregnancy, offers a reliable approach to timed mating in the guinea pig.

Applicability of emergency management theory in the management of Covid-19 health pandemic in Nigeria

Emergency has become a global reoccurrence and when it occurs in a country, the government usually adopt measures to curtail the negative effects of the phenomenon. COVID-19 as the present global emergency needs to be tackled with proper and effective management measures. The principles of Emergency Management Theory are among the global highly rated and most common measures used to tackle emergencies to achieve minimal effect on lives and economies. It is based on the above statements that this study examined the Application of the principles of Emergency Management Theory in the Federal Government of Nigeria‟s management of COVID-19 to ascertain the extent the Federal Government is utilizing the theory‟s principles in its fight against COVID-19 in Nigeria. The study utilized the secondary method of data collection and used observations and documents as its tools of evaluation. The study found that the principles of Emergency Management Theory have been applied by the Federal Government in the management of COVID-19 in Nigeria. The study concludes that the application of the principles Emergency Management Theory in the management of COVID-19 has greatly enhanced Government‟s efforts in the fight against the disease in Nigeria. To improve on emergency management in Nigeria, the study recommended that: Individual researchers and research institutes be supported to develop more proactive ways of dealing with emergencies in the country and Nigerians should all be re-orientated and sensitized on how to prevent, respond and deal with emergencies.

Innovation In Indian Hospitality Industry: A Reflection Of Culinary Perspectives

The hospitality and restaurant industry is changing rapidly, but silently and inevitable from many points of view. These innovations are motivated by demographic patterns, by the demands of modern consumers and are also influenced by the global economic crisis. Modern consumers and their ever-changing needs prompt response and service personalised to their needs. It is a specific sector due to several factors that affect the customer's view of experience, and hence innovation in this sector is a dynamic and multidimensional process. In order to succeed in a competitive market and make sustainable profits, the hospitality industry needs to be different for competitive advantage and innovation. When the various theoretical backgrounds on culinary innovation in hospitality sectors are checked, it is found that studies representing the views of the hotel and restaurant chefs and cooks are inadequate and many of them are also unaware of this particular word called “innovation”. Hence this paper tends to explore the various existing knowledge on food innovation and try to communicate it among the chefs and cooks working in hotels and restaurants. For this purpose, the secondary method of data collection has been initiated to find out through knowledge about it.

A Non-invasive Detection Technique of Adenocarcinoma with the Use of Streptavidin-Coated Iron Nanoparticles and Biotinylated-Antibodies and the Primary-Secondary Approach

The streptavidin and biotin interaction is one of the strongest non-covalent interactions in nature. As a result, this non-covalent interaction has been of great interest when it comes to biochemical assays, diagnosis of diseases, and cell-targeted drug delivery. Past research has proven that biotin-streptavidin is useful in biosensor development to improve the detection of a system when conjugated to nanoparticles. This study aims to prove that streptavidin-coated nanoparticles can be conjugated with biotinylated antibodies using the primary-secondary method to non-invasively detect adenocarcinoma in-vitro. While the use of nanoparticles is not uncommon to the diagnostics area of scientific research, the technique this research aims to investigate is a non-invasive one, utilizing the primary-secondary method. Specifically, the increased stability of fluorophores when bound to antibodies as opposed to nanoparticles directly can be indicative of the particles conjugated through the primary-secondary method’s ability to specifically bind to overexpressed transferrin receptors in the A549 cell line. In this paper, streptavidin-coated nanoparticles were conjugated with biotinylated anti-transferrin receptor antibodies and AlexaFluor-488 secondary antibodies were used to enable fluorescence-based detection. The efficiency of these particles were observed quantitatively through a plate reader and qualitatively through a fluorescence microscope. I demonstrated that these nanoparticles are able to specifically bind to the target proteins in this study. These findings contribute to the field of nanoparticle diagnostics and can be extended to different diseases caused by overexpression of proteins in the future. While this was conducted in-vitro, conjugates can be prepared to detect cancer in-vivo and can be tested with magnetic relaxometry in the future.

Between Magnanimity and Malevolence: Nigeria’s Commitment to South Africa’s Political Freedom in the Lens of Reciprocity

The history of South Africa’s long walk to political freedom is dotted with Nigeria’s undaunted commitment and involvement, propelled by Nigeria’s Afrocentric foreign policy stance. This study therefore, demonstrates Nigeria’s concern for Africa’s political liberation, and in particular, presents Nigeria’s commitment to South Africa’s struggle for political freedom during the colonial years. It adopts the secondary method of data collection, and borrows from the conceptual framework and doctrinal provisions of reciprocity to weigh South Africa’s attitude towards Nigeria’s commitment to her (South Africa’s) political emancipation. Passing Nigeria’s involvement in South Africa’s liberation struggle and South Africa’s treatments of Nigeria through the critical lens of historical and theoretical analysis, this study makes a finding that Nigeria’s magnanimity to South Africa is at variance with South Africa’s response to Nigeria. The study recommends that Nigeria’s relations with her African brothers, informed by her foreign policy of Afrocentrism, should reflect reciprocity. In sum, that in her foreign relations, Nigeria should treat as she is treated.

Symplectic P-stable additive Runge—Kutta methods

<p style='text-indent:20px;'>Classical symplectic partitioned Runge–Kutta methods can be obtained from a variational formulation where all the terms in the discrete Lagrangian are treated with the same quadrature formula. We construct a family of symplectic methods allowing the use of different quadrature formulas (primary and secondary) for different terms of the Lagrangian. In particular, we study a family of methods using Lobatto quadrature (with corresponding Lobatto IIIA-B symplectic pair) as a primary method and Gauss–Legendre quadrature as a secondary method. The methods have the same implicitness as the underlying Lobatto IIIA-B pair, and, in addition, they are <i>P-stable</i>, therefore suitable for application to highly oscillatory problems.</p>

THE DYNAMICS OF MANAGING THE RISING UNEMPLOYMENT HURDLES IN NIGERIA: A FOCUS ON IMO STATE

This study examines the causes and effects of unemployment in Nigeria with particular reference to Imo State. The National Bureau of Statistics (NBS) report on unemployment showed that the country unemployment rate increased to 27.1% in the second quarter of the year (Q2 2020) compared to 23.1% of the third quarter (Q3 2018). Imo State both in terms of unemployment and underemployment records 48.7% and 75.s% respectively. The report is worrisome as it poses threat to the State development, security and peaceful co-existence. The main objective is to look into the causes, effects and solution to unemployment problem. The study reviewed extant literature anchored on the theory of Neo-liberalism of Marxist. Also, historical and descriptive research design with secondary method as sources of its data collection were utilized, while content analysis was adopted. The result of the study reveals that unemployment is a multidimensional problem caused by holistic variables vis: corruption, ineptitude leadership, neglect of agriculture, weak infrastructure, with negative implication as poverty, low income and insecurity. The study recommends comprehensive approaches which include functional governance, provision of enabling environment and re-calibration of policies that will strengthen effective governance, investment and economic

Trade Union amendment act 2005 and the entrenchment of industrial democracy in the Nigeria Public Service: An x-ray of the political undercurrent

The Federal Government of Nigeria in 2005 amended some sections of the Trade Union Act referred to as “The Principal Act” 1996 to form the new Trade Union (Amendment) Act 2005. The main objective of the new Act was the entrenchment of democracy in Nigeria public service by giving workers the freedom to choose which union to belong and opportunity to form more central union organizations, thereby encouraging industrial democracy. It is on this note that this study examined the political underlying motive in enacting the new Act in relation to industrial democracy in Nigeria Public Service, the weaknesses and strength of the Act were examined. The study relied on the secondary method of data collection. The study concluded that the enactment of the Trade Union (Amendment) Act 2005 was politically motivated to give the government the much-needed space to implement certain policies and decisions that are comfortable for the government. The study then recommended that the governmentshould embark on nation-wide consultation with stakeholders to develop valid amendments in Trade Union Acts for the betterment of all and not for the exploitation of the masses. Keywords: Trade Union Amendment Act, industrial democracy and Public service.

A Review on the Criminal Justice System: Understanding its Structural Components and Process in Pakistan

Criminal justice system is fundamental to the cause of establishing a peaceful and nonviolent society; therefore, it is pertinent to understand criminal justice procedure in detail. This study was planned to know the procedure, structure and various parts involving in the criminal justice system. A secondary method of study was adopted by reviewing academic articles and books in order to review the criminal justice system. The article presents definitions of the criminal justice system, its components and also presents a detailed account of each component. Besides, the role and functions of different components including police, court procedures, and role of judges, bail process and correctional institutions were reviewed. It is concluded that criminal justice system has evolved and still evolving in the contemporary world including Pakistan and its complex elements may be made understandable to the common citizens while looking at the changing needs and problems in various times and spaces.

Time mating guinea pigs by monitoring changes to the vaginal membrane throughout the estrus cycle and with ultrasound confirmation

Guinea pig development in utero is more similar to humans than any other rodent species. As such, their importance to reproductive studies is evident, particularly those studies focused on therapeutic interventions to improve human pregnancy outcome. Tracking the guinea pig estrus cycle is imperative to ensuring appropriately timed mating and can be performed by monitoring the guinea pig vaginal membrane. Here, we describe, and provide picture representation, of changes to the guinea pig vaginal membrane throughout the estrus cycle. Utilization of this monitoring enabled a 100% pregnancy success rate on the first mating attempt in a cohort of five guinea pigs. This approach, along with early pregnancy ultrasounds as a secondary method to confirm pregnancy, offers a cost effective and reliable approach to timed-mating in the guinea pig.