EFEKTIVITAS ANTIMIKROBA BUAH LAKUM (Cayratia trifolia [L.] DOMIN) TERHADAP PERTUMBUHAN BAKTERI Streptococcus sp. (L.10.3)

Streptococcus sp. (L.10.3) is one of the bacteria that can cause dental plaque. This study was aimed to determine the antimicrobial activity of the fraction of methanol fraction of fruit against Streptococcus sp. (L.10.3). This study used a Completely Randomized Design (CRD), 3 levels of concentrations of fraction methanol fraction 0.3 g/mL, 0.6 g/mL and 0.9 g/mL and 2 positive control treatments of amoxicillin and negative control using distilled water with three times repeated. Testing the antibacterial activity using the Kirby-Bauer method using disc paper. The results of the study showed the average diameter of inhibition zones of Streptococcus sp. (L.10.3) in the 24th hour incubation ranged from 7.70-10.72 mm and in the 48th hour incubation ranged from 8.70-10.37 mm. A concentration of 0.9 g/mL is an effective concentration with a moderate inhibition response category. Giving fraction of methanol fraction of fruit at the lowest concentration of 0.3 g/mL is able to provide a moderate inhibition response with the bacteriocidal antibacterial category.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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UJI EFEKTIVITAS EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) SEBAGAI ANTIDIABETES TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽

10.35799/pha.10.2021.32767 ◽

2021 ◽

Vol 10 (1) ◽

pp. 736

Author(s):

Grace Laury Tulung ◽

Widdhi Bodhi ◽

Jainer Pasca Siampa

Keyword(s):

Rattus Norvegicus ◽

Design Method ◽

Centella Asiatica ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Flavonoid Compound ◽

Randomized Design ◽

Decrease Blood Glucose ◽

Completely Randomized Design

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords: Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus ABSTRAK Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus

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Penggunaan Kulit Nanas Fermentasi dalam Ransum yang Mengandung Gulma Berkhasiat Obat Terhadap Konsumsi Nutrient Ayam Broiler

Jurnal Agripet ◽

10.17969/agripet.v16i2.4142 ◽

2016 ◽

Vol 16 (2) ◽

pp. 76

Author(s):

Wasir Ibrahim ◽

Rita Mutia ◽

Nurhayati Nurhayati ◽

Nelwida Nelwida ◽

Berliana Berliana

Keyword(s):

Nutrient Intake ◽

Broiler Chicken ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Pineapple Peel ◽

Nutrient Consumption ◽

Before And After ◽

Completely Randomized Design ◽

And Performance

ABSTRAK. Penelitian ini bertujuan untuk mengetahui penggunaan kulit nanas fermentasi ke dalam ransum yang mengandung gulma berkhasiat obat terhadap konsumsi nutrient ayam broiler. Penelitian ini menggunakan DOC jantan strain cob umur 2 hari sebanyak 200 ekor yang dipelihara selama 42 hari. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. P0 = 0% tepung kulit nanas fermentasi (TKNF) dalam ransum mengandung 0% gulma obat(GO), (kontrol positif), P1 = 0% TKNF dalam ransum mengandung 2 % gulma obat (GO),(kontrol negatif), P2 = 7.5% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat, P3 = 15% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat, P4 = 22.5% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat. Parameter yang diamati kandungan gizi kulit nanas sebelum dan sesudah fermentasi dan konsumsi nutrient. Pengaruh yang nyata terhadap parameter yang diamati dilanjutkan dengan Uji Jarak Berganda Duncan, Data yang diperoleh dari setiap parameter dianalisis ragam (ANOVA) menggunakan bantuan software SPSS 16.0. Dari hasil penelitian disimpulkan bahwa penggunaan kulit nanas yang fermentasi dengan yoghurt dalam ransum yang mengandung gulma berkhasiat obat pada taraf 15% memberikan hasil terbaik pada konsumsi nutrient ayam broiler. (Fermented pineapple peel supplementation with addition of medicinal weeds on nutrient intake consumption of broiler chicken) ABSTRACT. The aim of this study was to determine the effect of using fermented pineapple peel in the ration containing medicinal weeds on nutrient consumption and performance of broiler . Two hundred 2 days male broiler chicken cob strain were used in this study and kept for 42 days. Completely randomized design (CRD)was applied with 5 treatments and 5 replicates. Treatments consisted of P0 = 0% of fermented pineapple peels meal (FPPM) in ration containing 0% medicinal weed (MW), positive control). P1 = 0% of fermented pineapple peels meal (FPPM) in the ration containing 2 % medicinal weed (MW), negative control). P2 = 7.5% of FPPM in the ration containing 2 % medicinal weed (MW). P3 = 15% of FPPM in the ration containing 2 % medicinal weed (MW). P4 = 22,5% of FPPM in the ration containing 2 % medicinal weed (MW). The parameters measured was nutrien intake before and after fermented peel and consumption. The observed data were analyzed by statistical product and service solution (SPSS 16.0). Results showed that use of fermented pineapple peel meal up to 15% with addition medicinal weeds was the best on nutrient consumption of broiler chicken.

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Probiotic and synbiotic in broiler diet: performance and Enterobacteriaceae

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-12035 ◽

2020 ◽

Vol 72 (6) ◽

pp. 2365-2372

Author(s):

P.R.S.C. Leite ◽

H.B. Oliveira ◽

V.B.L. Souza ◽

F.O. Rocha ◽

T.H. Oliveira

Keyword(s):

Broiler Chickens ◽

Positive Control ◽

Negative Control ◽

Broiler Chicks ◽

Broiler Performance ◽

Randomized Design ◽

Colony Forming Units ◽

Completely Randomized Design ◽

Zinc Bacitracin ◽

Experimental Treatments

ABSTRACT The objective of this study was to evaluate the effects of probiotics and synbiotics on the performance and Enterobacteriaceae count of broiler chickens. A total of 640 one-day-old male broiler chicks were distributed in a completely randomized design with four treatments and eight replicates with 20 birds each. The treatments were: ration with performance enhancer (zinc bacitracin; positive control); ration without performance enhancer and probiotic/synbiotic (negative control); ration with probiotics; and ration with synbiotics. At 35 days, five birds from each treatment were euthanized and intestinal contents were harvested for determining the Enterobacteriaceae count. The performance data and average colony-forming units (CFUs) transformed as log CFU/g were subjected to analysis of variance and Tukey’s test. The effects of probiotics and synbiotics were observed in the initial phase, with supplemented birds exhibiting comparable weight gain to those supplemented with bacitracin. No effect of the treatment on broiler performance was observed after 42 days. The enterobacterial count was comparable among all experimental treatments. Supplementation with probiotics and synbiotics did not compromise the performance of broilers and did not alter the Enterobacteriaceae count.

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Effects of alphamune G on the performance, serum and haematological parameters of Escherichia coli challenged turkey poults

Nigerian Journal of Animal Production ◽

10.51791/njap.v40i2.1137 ◽

2020 ◽

Vol 40 (2) ◽

pp. 112-121

Author(s):

S. A. Bolu ◽

M. T. Adelakun

Keyword(s):

Escherichia Coli ◽

Poor Performance ◽

Positive Control ◽

Negative Control ◽

E Coli ◽

Turkey Poults ◽

Randomized Design ◽

Completely Randomized Design ◽

Improved Performance ◽

Haematological Indices

A study was conducted to determine the response of Turkey poults to graded levels of Alphamune G (0.00+, 0.03, 0.04, 0.05, 0.06 and 0.00 %-) when challenged with Escherichia coli orally for 7 days. The graded levels were the treatments viz 0.00%+ (positive control), Alphamune G at 0.03, 0.04, 0.05, 0.06% and 0.00%- (negative control; infected without Alphamune G supplementation). Each treatment was allotted 3 replicates of 6 poults. The experiment which was conducted for 56 days employed a completely randomized design. E. coli was isolated from the intestinal digesta of a colisepticaemic chicken. 108 turkey poults were used in this study. Poults were infected with E.coli for 7 days through the drinking water and given the treatment. The performance parameters of Alphamune G supplementation were significantly affected. The cumulative weight, Feed intake and weight gain were highest for turkey poults fed 0.06% Alphamune G supplementation. These values were also directly proportional to the supplementation levels of Alphamune G. The birds given the negative treatment (0.00 %-) had relatively poor performance compared to the other treatments. The specific enzymes studied were significantly affected (p<0.05) by the treatments. ALT and AST were significantly highest for turkey poults fed the negative control. Enzyme values became optimum at 0.05% Alphamune G supplementation. At 0.06% of Alphamune G supplementation, cellular mitigations of the effects of E. coli was measurable. Urea and creatinine were not significantly (p>0.05) influenced by the treatments. Haematological indices such as WBC and specific differential counts (lymphocytes and neutrophils) were affected significantly (P<0.05) by supplemental levels of Alphamune G The Inclusion of Alphamune G at 0.06% in the diets improved performance of turkey poults when challenged with Escherichia coli.

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Aktivitas Antifungi Ekstrak Etanol Daun Eryngium foetidum L. Terhadap Pertumbuhan Xeromyces sp. secara in vitro

Jurnal Protobiont ◽

10.26418/protobiont.v8i2.34056 ◽

2019 ◽

Vol 8 (2) ◽

Author(s):

Ines S Simatupang Elvi Rusmiyanto PW, Rikhsan Kurniatuhadi

Keyword(s):

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Activity Level ◽

Piper Nigrum ◽

Dilution Method ◽

Randomized Design ◽

Eryngium Foetidum ◽

Completely Randomized Design

Eryngium foetidum is a plant commonly used a seasoning cuisine and has a variety of chemical compounds that potentially an antifungal. The ethanol extract Eryngium foetidum was tested against fungal isolate Xi.01. The isolat Xi.01 isolated from the pepper (Piper Nigrum) stem was identified as Xeromyces sp. This study aimed to determine the ability of the antifungal ethanol extract of Eryngium foetidum against Xeromyces sp. (Xi.01). This study used solid dilution method and completely randomized design using 18 treatments, i.e negative control, positive control, diethanolamide concentration of 2.5; 5; 7.5 and 10% combined with the ethanol extract concentration of Eryngium foetidum of 5; 10; 20 and 40%. The results showed 16 treatment combinations had the same low antifungal activity level in the range 13,59-22,40%

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Effectivity of Trichoderma harzianum as Bio-fungicide Against Moler Disease and Bio-stimulator of Shallot Growth

Journal of Agri-Food Science and Technology ◽

10.12928/jafost.v1i1.1941 ◽

2020 ◽

Vol 1 (1) ◽

pp. 12

Author(s):

Icha Rusita ◽

Hadi Sasongko

Keyword(s):

Trichoderma Harzianum ◽

Dry Weight ◽

Positive Control ◽

Negative Control ◽

Correlation Pattern ◽

Randomized Design ◽

Significant Difference ◽

Wet Weight ◽

Number Of Leaves ◽

Completely Randomized Design

Moler disease is the primary disease that disturbs the shallot’ cultivation. Hence, it is beneficial to have preventive measures to mitigate the risk and to improve growth. The research objective is to know the effectivity of Trichoderma harzianum suspension as a bio-fungicide and bio-stimulator. The study used seven treatments, i.e., immersion of shallot seeds on negative control (distilled water), positive control (synthetic fungicide+ZPT 1g/liter), T.harzianum suspension with different concentrations of 2 ppm, 4 ppm, 6 ppm, 8 ppm, and 10 ppm, assigned in a Completely Randomized Design (CRD). Treatment that results in a significant level will be continued to the Least Significance Different (LSD) 5% test. The observed variables are the intensity of moler disease and the growth of shallot seeds. Experiment results show the significant difference between treatments. The optimal treatment to reduce the percentage of moler disease intensity is the immersion of shallot seeds in 10 ppm-concentrated T.harzianum suspension, while to improve the shallot growth is in 8 ppm-concentrated T.harzianum suspension. The intensity of moler disease has a negative correlation to the plant’s height and leaf’s diameter but shows no correlation pattern to the number of leaves, wet weight, dry weight, and length of roots.

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Xylanase and β-glucanase in maize- and soybean meal-based diets for broilers

Semina Ciências Agrárias ◽

10.5433/1679-0359.2020v41n6supl2p3259 ◽

2020 ◽

Vol 41 (6supl2) ◽

pp. 3259-3274

Author(s):

Thiago Ferreira Diana ◽

◽

Luiz Fernando Teixeira Albino ◽

Horácio Santiago Rostagno ◽

Bruno Figueiredo de Almeida ◽

...

Keyword(s):

Soybean Meal ◽

Production Efficiency ◽

Efficiency Index ◽

Positive Control ◽

Negative Control ◽

Metabolizable Energy ◽

Experimental Unit ◽

Feed Conversion ◽

Randomized Design ◽

Completely Randomized Design

The objective of this study was to evaluate the effect of adding different xylanase and ?-glucanase enzyme blends to maize- and soybean meal-based diets on performance and energy metabolizability in broilers. Two experiments were carried out with broilers of the COBB 500 strain. In the first experiment, 1960 chicks were assigned to a completely randomized design with a 2 × 3 + 1 factorial arrangement, totaling seven treatments, namely, T1- Positive control (PC); T2 - Negative control 1 (NC1; PC minus 200 kcal kg-1 ME); T3 - NC1 + Blend A; T4 - NC1 + Blend B; T5 - Negative control 2 (NC2; PC minus 167 kcal kg-1 ME and 5% amino acids); T6 - NC2 + Blend A; and T7 - NC2 + Blend B. Fourteen replicates were used per treatment and 20 birds per experimental unit. The parameters evaluated at 21 and 42 days of age were weight gain (WG), feed intake (FI) and feed conversion (FC). At 42 days, production efficiency index (PEI), viability and the yields of cuts were also calculated. Birds that received diets with a reduced nutritional value showed a reduction in WG and PEI and worsened FC as compared those of PC treatment (p < 0.05). However, the birds that consumed the NC2 diet with Blend B exhibited a similar WG to those in PC group (p > 0.05) from 1 to 21 days of life. For the yield of thigh + drumstick, the factors were statistically similar (p > 0.05) to those observed in the PC birds. In the second experiment, 432 fourteen-day-old chicks were distributed in a completely randomized design with seven treatments, with eight replicates per treatment and six birds per experimental unit. The apparent metabolizable energy (AME) and nitrogen-corrected AME (AMEn) values were determined. Overall, the NC2 diet with Blend B provided the highest AME and AMEn values; however, NC1 with the same enzyme blend was the treatment which provided the lowest values. The addition of xylanase and ?-glucanase enzyme blends to maize- and soybean meal-based diets improves WG at 21 days as well as PEI in broilers; however, it does not influence the yield of cuts. Enzymes (Blend B) improve the energy metabolization of broiler diets with reduced energy and amino acid levels.

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Evaluation of integrated sludge worm and catfish farming with biofloc system

Jurnal Akuakultur Indonesia ◽

10.19027/jai.13.28-35 ◽

2015 ◽

Vol 13 (1) ◽

pp. 28

Author(s):

Dedi Pardiansyah ◽

Eddy Supriyono ◽

Daniel Djokosetianto

Keyword(s):

Culture Media ◽

Positive Control ◽

Negative Control ◽

Chicken Manure ◽

Biomass Growth ◽

Fish Hatchery ◽

Randomized Design ◽

Completely Randomized Design ◽

System Water ◽

Intensive System

ABSTRACTCurrently, supply of sludge worm (Tubifex sp.) merely came from wild, so that it could not meet demand for fish hatchery. Additionally, harvest from the wild did not have a quality assurance, due to the possibility of sludge worm becoming an agent of disease. This study was conducted to evaluate the production of sludge worm culture by utilizing catfish culture waste (Clarias sp. ) in bioflok system. In this system, water from catfish culture media flowed into sludge worm culture media using recirculation systems. This study used a completely randomized design with four treatments and two replications, in which treatment A (addition of catfish culture waste from intensive system), treatment B (addition of catfish culture waste from biofloc system), treatment C (the addition of fermented chicken manure at the beginning of experiment as negative control), and treatment D (addition of fermented chicken manure at the beginning of experiment and then every five days as positive control). The results showed that the highest production was obtained by treatment B at biomass growth of 0.97 kg/m2 and sludge worm density of 388.000 individu/m2. Keywords: sludge worm, biofloc, chicken manure, catfish, catfish culture waste ABSTRAK Saat ini pasokan cacing sutra (Tubifex sp.) hanya berasal dari alam, sehingga belum mencukupi permintaan untuk kegiatan pembenihan ikan. Selain itu, hasil tangkapan dari alam tidak memiliki jaminan kualitas, karena cacing sutra dapat menjadi agen penyakit. Penelitian ini dilakukan untuk mengevaluasi hasil produksi budidaya cacing sutra dengan memanfaatkan limbah budidaya ikan lele (Clarias sp.) sistem bioflok. Pada sistem ini, air dari media budidaya ikan lele dialirkan ke media pemeliharaan cacing sutra menggunakan sistem resirkulasi. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan dan dua ulangan, yaitu perlakuan A (pemberian limbah ikan lele sistem intensif), perlakuan B (pemberian limbah ikan lele sistem bioflok), perlakuan C (penambahan fermentasi kotoran ayam pada awal penelitian sebagai kontrol negatif), serta perlakuan D (penambahan fermentasi kotoran ayam pada awal penelitian yang dilanjutkan setiap lima hari sekali sebagai kontrol positif). Hasil penelitian menunjukkan bahwa produksi cacing sutra tertinggi diperoleh pada perlakuan B dengan pertumbuhan bobot sebesar 0,97 kg/m2 dan kepadatan cacing sutra sebesar 388.000 individu/m2. Kata kunci : cacing sutra, bioflok, kotoran ayam, ikan lele, limbah budidaya ikan lele

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PENGARUH EKSTRAK Sargassum cristaefolium PADA MULTIPLIKASI Dendrobium antennatum Rchb.f SECARA IN VITRO

Bioscientist : Jurnal Ilmiah Biologi ◽

10.33394/bjib.v8i1.2672 ◽

2020 ◽

Vol 8 (1) ◽

pp. 18

Author(s):

Faisal Ansyarif ◽

Mursal Ghazali ◽

Aida Muspiah ◽

Rina Kurnianingsih

Keyword(s):

Culture Media ◽

Positive Control ◽

Negative Control ◽

Growth Media ◽

Extract Concentration ◽

Tissue Culture Media ◽

Randomized Design ◽

Completely Randomized Design ◽

Natural Cytokinin

The purpose of this study was to determine the potential and concentration of Sargassum cristaefolium extract as a natural cytokinin in tissue culture media of Dendrobium antennatum Rchb.f. This study is experimental with a completely randomized design, using several extract concentrations compared with the positive control (BAP 1.5 ppm) and negative control (MS0 media). Extract concentrations used 5 ppm, 10 ppm, 15 ppm, 20 ppm, and 25 ppm. Based on analysis of variance (ANOVA), the effect of Sargassum cristaefolium extract on the growth media significant on all parameters. Sargassum cristaefolium extracts caused different responses at certain levels of concentration. Extract concentration of 10 ppm was able to initiate the highest number of shoots and leaves compared to other extract concentrations, where as the concentration 20 ppm was able to accelerate and increase root growth.

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