Analytical Strategies and Applications in Lipidomics

2022 ◽  
pp. 1-26
Author(s):  
Weronika Hewelt-Belka ◽  
Agata Kot-Wasik
Keyword(s):  
Analytical Strategies

Effects of Psychotherapy on Brain Activation Patterns in Anxiety Disorders

2016 ◽  
Vol 224 (2) ◽  
pp. 62-70 ◽  
Author(s):  
Thomas Straube
Keyword(s):  
Anxiety Disorders ◽  
Neuronal Plasticity ◽  
Functional Neuroimaging ◽  
State Of The Art ◽  
Methodological Issues ◽  
Future Directions ◽  
Activation Patterns ◽  
New Learning ◽  
Analytical Strategies ◽  
Sample Characteristics

Abstract. Psychotherapy is an effective treatment for most mental disorders, including anxiety disorders. Successful psychotherapy implies new learning experiences and therefore neural alterations. With the increasing availability of functional neuroimaging methods, it has become possible to investigate psychotherapeutically induced neuronal plasticity across the whole brain in controlled studies. However, the detectable effects strongly depend on neuroscientific methods, experimental paradigms, analytical strategies, and sample characteristics. This article summarizes the state of the art, discusses current theoretical and methodological issues, and suggests future directions of the research on the neurobiology of psychotherapy in anxiety disorders.

scholarly journals Analyzing Failed Institutional Change Attempts

2021 ◽  
pp. 106591292198944
Author(s):  
Sukriti Issar ◽  
Matthias Dilling
Keyword(s):  
Institutional Change ◽  
Comparative Politics ◽  
Change Agents ◽  
The United States ◽  
Coalition Building ◽  
Strategic Action ◽  
Formal Stability ◽  
Political Effects ◽  
Analytical Strategies ◽  
Empirical Regularity

Theoretical advances in the study of institutional change center around a productive paradox. While change agents can take strategic action to change institutions, institutions display a remarkable level of formal stability. From this paradox, we expect that attempts to change institutions are an empirical regularity and that many formal change attempts will fail. This article contributes to historical institutionalism by analyzing the political effects of failed formal institutional change attempts on institutional sequences. Failed institutional change attempts could be mere blips, having little effect on subsequent institutional trajectories, or even inoculate against future attempts. Failed attempts could also lay the ideational groundwork, aid in coalition building, and garner concessions for subsequent institutional change, or convince change agents to alter their strategy. The article suggests analytical strategies to assess the effects of failed institutional change attempts, drawing on examples from comparative politics and two extended case illustrations from Italian party politics and the Affordable Care Act in the United States.

Mass spectrometry as a versatile ancillary technique for the rapid in situ identification of lichen metabolites directly from TLC plates

2017 ◽  
Vol 49 (5) ◽  
pp. 507-520 ◽  
Author(s):  
Pierre LE POGAM ◽  
Aline PILLOT ◽  
Françoise LOHEZIC-LE DEVEHAT ◽  
Anne-Cécile LE LAMER ◽  
Béatrice LEGOUIN ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Accurate Identification ◽  
Chemical Profiling ◽  
Analytical Strategies ◽  
Tlc Plates ◽  
Lichen Metabolites ◽  
Acetone Extracts ◽  
Layer Chromatography ◽  
Structural Classes

AbstractThin-layer chromatography (TLC) still enjoys widespread popularity among lichenologists as one of the fastest and simplest analytical strategies, today remaining the primary method of assessing the secondary product content of lichens. The pitfalls associated with this approach are well known as TLC leads to characterizing compounds by comparison with standards rather than properly identifying them, which might lead to erroneous assignments, accounting for the long-held interest in hyphenating TLC with dedicated identification tools. As such, commercially available TLC/Mass Spectrometry (MS) interfaces can be easily connected to any brand of mass spectrometer without adjustments. The spots of interest are extracted from the TLC plate to retrieve mass spectrometric signals within one minute, thereby ensuring accurate identification of the chromatographed substances. The results of this hyphenated strategy for lichens are presented here by 1) describing the TLC migration and direct MS analysis of single lichen metabolites of various structural classes, 2) highlighting it through the chemical profiling of crude acetone extracts of a set of lichens of known chemical composition, and finally 3) applying it to a lichen of unknown profile, Usnea trachycarpa.

The Discovery Channel: microfluidics and microengineered systems in drug screening

2015 ◽  
Vol 7 (3) ◽  
pp. 285-288 ◽  
Author(s):  
Christopher Moraes
Keyword(s):  
Drug Discovery ◽  
Engineering Design ◽  
High Throughput ◽  
Drug Screening ◽  
High Throughput Screening ◽  
Recent Literature ◽  
Analytical Strategies ◽  
Discovery Pipeline

We highlight exciting findings and promising approaches in the recent literature in which researchers integrate advanced micro-engineering, design, and analytical strategies to improve the relevance and utility of high-throughput screening in the drug discovery pipeline.

scholarly journals Multidimensional Analytical Characterization of Water-Soluble Organic Aerosols: Challenges and New Perspectives

2021 ◽  
Vol 11 (6) ◽  
pp. 2539
Author(s):  
Regina M. B. O. Duarte ◽  
João T. V. Matos ◽  
Armando C. Duarte
Keyword(s):  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Organic Aerosols ◽  
Analytical Techniques ◽  
Water Soluble ◽  
Chemical Complexity ◽  
Molecular Features ◽  
High Resolution Mass ◽  
Analytical Strategies ◽  
Resolution Mass

Water-soluble organic aerosols (OA) are an important component of air particles and one of the key drivers that impact both climate and human health. Understanding the processes involving water-soluble OA depends on how well the chemical composition of this aerosol component is decoded. Yet, obtaining detailed information faces several challenges, including water-soluble OA collection, extraction, and chemical complexity. This review highlights the multidimensional non-targeted analytical strategies that have been developed and employed for providing new insights into the structural and molecular features of water-soluble organic components present in air particles. First, the most prominent high-resolution mass spectrometric methods for near real-time measurements of water-soluble OA and their limitations are discussed. Afterward, a special emphasis is given to the degree of compositional information provided by offline multidimensional analytical techniques, namely excitation–emission (EEM) fluorescence spectroscopy, high-resolution mass spectrometry and two-dimensional nuclear magnetic resonance (NMR) spectroscopy and their hyphenation with chromatographic systems. The major challenges ahead on the application of these multidimensional analytical strategies for OA research are also addressed so that they can be used advantageously in future studies.

scholarly journals The Trials and Tribulations of Applied Triangulation: Weighing Different Data Sources

2016 ◽  
Vol 12 (2) ◽  
pp. 166-181 ◽  
Author(s):  
Florian G. Kern
Keyword(s):  
Quantitative Research ◽  
Political Power ◽  
Data Sources ◽  
Qualitative And Quantitative ◽  
Practical Research ◽  
The Status ◽  
Analytical Strategies ◽  
Depth Interviews ◽  
Shed Light ◽  
Illustrative Application

Researchers employ triangulation to increase the validity of inference in qualitative and quantitative research. Leuffen, Shikano, and Walter have presented guidance as to which strategies to use when triangulating data sources. In this article, I explore how their findings can be translated for practical research purposes. I offer an illustrative application concentrating on the political power of traditional political authorities in Uganda and Tanzania. I analyze the status quo of political power and the preferred political power of traditional leaders. To triangulate, I use three sources: (1) constitutional-legal texts, (2) the Afrobarometer survey, and (3) in-depth interviews. I shed light on possible problems and analytical strategies for triangulation in practice, with a specific focus on convergence and divergence of sources.

scholarly journals Open access resources for genome-wide association mapping in rice

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Susan R. McCouch ◽  
Mark H. Wright ◽  
Chih-Wei Tung ◽  
Lyza G. Maron ◽  
Kenneth L. McNally ◽  
...  
Keyword(s):  
Open Access ◽  
Association Mapping ◽  
High Density ◽  
Genome Wide Association ◽  
Minor Effect ◽  
Data Set ◽  
Genome Wide ◽  
Analytical Strategies ◽  
Biological Interpretation ◽  
Rice Improvement

Abstract Increasing food production is essential to meet the demands of a growing human population, with its rising income levels and nutritional expectations. To address the demand, plant breeders seek new sources of genetic variation to enhance the productivity, sustainability and resilience of crop varieties. Here we launch a high-resolution, open-access research platform to facilitate genome-wide association mapping in rice, a staple food crop. The platform provides an immortal collection of diverse germplasm, a high-density single-nucleotide polymorphism data set tailored for gene discovery, well-documented analytical strategies, and a suite of bioinformatics resources to facilitate biological interpretation. Using grain length, we demonstrate the power and resolution of our new high-density rice array, the accompanying genotypic data set, and an expanded diversity panel for detecting major and minor effect QTLs and subpopulation-specific alleles, with immediate implications for rice improvement.

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