scholarly journals Contamination of surgical mask during aerosol-producing dental treatments

2020 ◽  
Author(s):  
Madline Gund ◽  
Jonas Isack ◽  
Matthias Hannig ◽  
Sigrid Thieme-Ruffing ◽  
Barbara Gärtner ◽  
...  
Keyword(s):  
Outer Surface ◽  
Bacterial Contamination ◽  
Dental Treatment ◽  
Treated Patient ◽  
Treatment Modalities ◽  
Sterile Glove ◽  
Surgical Masks ◽  
Colony Forming Units ◽  
Dental Staff ◽  
Treatment Procedures

Abstract Objectives Surgical masks are usually contaminated during dental treatment. So far it has not been investigated whether a surgical mask itself can be a source of microbial transmission. The aim of this study was therefore to investigate the microbiological contamination of surgical masks during dental treatment and the transfer of microorganisms from the mask to the hands. Materials and methods Five dental treatment modalities were studied: carious cavity preparation (P-caries, n = 10), tooth substance preparation (P-tooth, n = 10), trepanation and root canal treatment (P-endo, n = 10), supragingival ultrasonic application (US-supra, n = 10), and subgingival periodontal ultrasonic instrumentation (US-sub, n = 10). Bacterial contamination of mask and gloves worn during treatment was tested by imprinting on agar plates. Additionally, before masks were tested, their outer surface was touched with a new sterile glove. This glove was also imprinted on agar. Bacteria were identified by MALDI TOF mass spectrometry. Colony-forming units (CFU) were scored: score 0: 0 CFU, score 1: < 102 CFU, score 2: > 102 CFU, score 3: dense microbial growth. Results All masks and all gloves used during treatment displayed bacterial contamination (sample scores 0/1/2/3: masks 0/46/3/1 and gloves 0/31/10/9). After touching the masks with new sterile gloves, microorganisms were recovered with the following contamination scores: P-caries: 4/6/0/0, P-tooth: 2/8/0/0: P-endo: 7/3/0/0, US-supra: 0/9/1/0, US-sub: 2/8/0/0. No statistically significant differences were detected between the treatment modalities. Streptococci spp. and Staphylococci spp. representing the oral and cutaneous flora dominated. Conclusions Surgical masks are contaminated after aerosol-producing dental treatment procedures. Used masks have a potential to be a source of bacterial contamination of the hands. Clinical relevance Dental staff should avoid touching the outer surface of masks with their hands to prevent transmission of pathogens. It is recommendable to change the mask after each treated patient followed by hand disinfection.

scholarly journals COVID-19: Prosthodontic Challenges and Opportunities in Dental Practice

2020 ◽  
Vol 11 (2) ◽  
pp. 113-116
Author(s):  
Dinesh Rokaya
Keyword(s):  
High Risk ◽  
Infection Control ◽  
Dental Treatment ◽  
Standards Of Care ◽  
Dental Practice ◽  
Dental Staff ◽  
Challenges And Opportunities ◽  
Treatment Procedures ◽  
Dental Assistants ◽  
Very High

The coronavirus disease (COVID-19) has spread globally. Dentists, dental specialists, dental assistants, dental staff, and patients are potentially at higher risk of COVID-19 infection during dental treatments. The prosthodontic dental treatment procedures may range from low to very high risk of COVID-19. This article presents necessary recommendations regarding the prosthodontic dental treatment procedures with standards of care and infection control.

Restorative dental treatment in times of COVID-19

2020 ◽  
Vol 68 ◽  
Author(s):  
Robson de Lima GOMES ◽  
Marlus da Silva PEDROSA ◽  
Claudio Heliomar Vicente da SILVA
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Dental Treatment ◽  
Close Contact ◽  
Cross Contamination ◽  
Clinical Protocols ◽  
Know How ◽  
Dental Procedures ◽  
Dental Staff ◽  
Dental Professional ◽  
Restrictive Measures

ABSTRACT Since the outbreak of the Coronavirus Disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), numerous restrictive measures have been adopted by governments of different countries. The return to elective dental care in Brazil is a reality even during the COVID-19 pandemic. During restorative dental procedures, the dental professional requires close contact with the patient, being exposed to contaminated saliva and fluids. In addition, transmission of COVID-19 by the generation of aerosol produced by dental handipieces may be possible. Thus, the dental staff must know how to act during restorative dental procedures, putting into practice the correct clinical protocols to avoid cross-contamination and COVID-19 spread. The purpose of this article is to review the literature on the biosafety practices especially in the context of restorative dental procedures in times of COVID-19.

scholarly journals Evaluation of the Most Stressful Dental Treatment Procedures of Conservative Dentistry among Polish Dental Students

2021 ◽  
Vol 18 (9) ◽  
pp. 4448
Author(s):  
Katarzyna Mocny-Pachońska ◽  
Rafał J. Doniec ◽  
Sylwia Wójcik ◽  
Szymon Sieciński ◽  
Natalia J. Piaseczna ◽  
...  
Keyword(s):  
Dental Treatment ◽  
High Stress ◽  
Dental Students ◽  
Endodontic Treatment ◽  
Stressful Environment ◽  
Caries Treatment ◽  
Dental Procedures ◽  
Conservative Dentistry ◽  
High Level ◽  
Treatment Procedures

Background: Dental schools are considered to be a very stressful environment; the stress levels of dental students are higher than those of the general population. The aim of this study was to assess the level of stress among dental students while performing specific dental procedures. Methods: A survey was conducted among 257 participants. We used an original questionnaire, which consisted of 14 questions assigned to three categories: I—Diagnosis, II—Caries Treatment, and III—Endodontic Treatment. Each participant marked their perceived level of stress during the performed dental treatment procedures. The scale included values of 0–6, where 0 indicates no stress, while 6 indicates high stress. Results: Third- (p=0.006) and fourth-year (p=0.009) women were characterized by a higher level of perceived stress during dental procedures related to caries treatment. Caries treatment procedures were the most stressful for 18.3% of third-year students, 4.3% of fourth-year students, and 3.2% of fifth-year students. Furthermore, 63.4% of third-year students, 47.3% of fourth-year students, and 17.2% of fifth-year students indicated that they felt a high level of stress when performing endodontic procedures. Conclusion: Third- and fourth-year female students are characterized by a higher level of stress during caries and endodontic treatment procedures. The most stressful treatments for participants were endodontic treatment procedures.

scholarly journals Susceptibility of Virulent Yersinia pestis Bacteria to Predator Bacteria in the Lungs of Mice

2018 ◽  
Vol 7 (1) ◽  
pp. 2 ◽  
Author(s):  
Riccardo Russo ◽  
Irina Kolesnikova ◽  
Thomas Kim ◽  
Shilpi Gupta ◽  
Androulla Pericleous ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Bacterial Infections ◽  
Treatment Modalities ◽  
The Novel ◽  
Global Public Health ◽  
Antibiotic Resistant ◽  
Colony Forming Units ◽  
Tier 1 ◽  
Predatory Bacteria ◽  
Lethal Doses

Multi-drug resistant bacterial infections are a serious threat to global public health. Changes in treatment modalities and prudent use of antibiotics can assist in reducing the threat, but new approaches are also required for untreatable cases. The use of predatory bacteria, such as Bdellovibrio bacteriovorus, is among the novel approaches being considered as possible therapeutics for antibiotic resistant and/or unidentified bacterial infections. Previous studies have examined the feasibility of using predatory bacteria to reduce colony-forming units (CFUs) in the lungs of rats exposed to lethal doses of Klebsiella pneumoniae; here we apply the approach to the Tier 1 select agent Yersinia pestis, and show that three doses of B. bacteriovorus introduced every six hours reduces the number of CFUs of Y. pestis in the lungs of inoculated mice by 86% after 24 h of infection. These experiments further demonstrate that predatory bacteria may serve to combat Gram negative bacterial infections, including those considered potential bioweapon agents, in the future.

Effectiveness of antimicrobial hospital curtains on reducing bacterial contamination—A multicenter study

2018 ◽  
Vol 40 (2) ◽  
pp. 164-170 ◽  
Author(s):  
Shik Luk ◽  
Viola Chi Ying Chow ◽  
Kelvin Chung Ho Yu ◽  
Enoch Know Hsu ◽  
Ngai Chong Tsang ◽  
...  
Keyword(s):  
Multicenter Study ◽  
Bacterial Contamination ◽  
Indirect Costs ◽  
Multidrug Resistant ◽  
Clinical Settings ◽  
Carbapenem Resistant ◽  
Colony Forming Units ◽  
Multidrug Resistant Organisms ◽  
Bed Days

AbstractObjectiveTo determine the efficacy of 2 types of antimicrobial privacy curtains in clinical settings and the costs involved in replacing standard curtains with antimicrobial curtains.DesignA prospective, open-labeled, multicenter study with a follow-up duration of 6 months.SettingThis study included 12 rooms of patients with multidrug-resistant organisms (MDROs) (668 patient bed days) and 10 cubicles (8,839 patient bed days) in the medical, surgical, neurosurgical, orthopedics, and rehabilitation units of 10 hospitals.MethodCulture samples were collected from curtain surfaces twice a week for 2 weeks, followed by weekly intervals.ResultsWith a median hanging time of 173 days, antimicrobial curtain B (quaternary ammonium chlorides [QAC] plus polyorganosiloxane) was highly effective in reducing the bioburden (colony-forming units/100 cm2, 1 vs 57; P < .001) compared with the standard curtain. The percentages of MDRO contamination were also significantly lower on antimicrobial curtain B than the standard curtain: methicillin-resistant Staphylococcus aureus, 0.5% vs 24% (P < .001); carbapenem-resistant Acinetobacter spp, 0.2% vs 22.1% (P < .001); multidrug-resistant Acinetobacter spp, 0% vs 13.2% (P < .001). Notably, the median time to first contamination by MDROs was 27.6 times longer for antimicrobial curtain B than for the standard curtain (138 days vs 5 days; P = .001).ConclusionsAntimicrobial curtain B (QAC plus polyorganosiloxane) but not antimicrobial curtain A (built-in silver) effectively reduced the microbial burden and MDRO contamination compared with the standard curtain, even after extended use in an active clinical setting. The antimicrobial curtain provided an opportunity to avert indirect costs related to curtain changing and laundering in addition to improving patient safety.

scholarly journals The impact of bacterial contamination of the ejaculate and extender on the quality of swine semen doses

2017 ◽  
Vol 38 (5) ◽  
pp. 3095 ◽  
Author(s):  
Ana Maria Groehs Goldberg ◽  
Marisa Cardoso ◽  
Mari Lourdes Bernardi ◽  
Ivo Wentz ◽  
Fernando Pandolfo Bortolozzo
Keyword(s):  
Bacterial Contamination ◽  
Sperm Morphology ◽  
No Reduction ◽  
Degree Of Contamination ◽  
Colony Forming Units ◽  
Semen Processing ◽  
Semen Extender ◽  
Contamination Levels ◽  
The Impact

The aim of this study was to verify the influence of the degree of bacterial contamination of boar ejaculate and semen extender on the quality of semen doses. The experiment was conducted in four boar studs, from which raw semen and two semen doses from each ejaculate were collected to evaluate the number of colony-forming units (CFU), pH, sperm morphology and motility. Extender samples were also evaluated for CFU. Ejaculates that had higher levels of contamination ( > 220 CFU mL-1) resulted in semen doses with a greater degree of bacterial contamination but with no reduction in motility or alteration in pH. When the semen doses were classified according to the degree of contamination of the extender, a decrease in motility was observed after 108 and 168 h of storage (P < 0.05) in the group whose extender had ? 14,000 CFU mL-1 versus the group whose extender had ? 330 CFU mL-1. The pH remained stable during 168 h of storage in semen doses with extender that had lower contamination levels, but decreased from 7.2 to 6.0 between 24 and 168 h of storage (P < 0.05) in the group with extender that had higher levels of contamination. A higher number of abnormal acrosomes (P < 0.05) were observed after 168 h of storage in the semen doses whose extender was highly contaminated. The production of semen doses with low bacterial contamination and high sperm cell viability will only be possible with a strict hygienic control in semen processing, primarily with respect to the extender, combined with minimal contamination during collection.

scholarly journals A Framework for Equity Access to Primary Dental Care

2020 ◽  
Vol 25 (9) ◽  
pp. 3669-3676
Author(s):  
Denise Silveira Antunes ◽  
Sudeshni Naidoo ◽  
Neil Myburgh ◽  
Juliana Balbinot Hilgert ◽  
Paul Douglas Fisher ◽  
...  
Keyword(s):  
Dental Care ◽  
Dental Treatment ◽  
Waiting Times ◽  
Face Validity ◽  
Oral Disease ◽  
Care Practice ◽  
Dental Services ◽  
Dental Staff ◽  
Dental Rehabilitation ◽  
Oral Disease Prevention

Abstract The objective of this study was to demonstrate face validity with a novel resource allocation framework designed to maximize equity into dental booking systems. The study was carried out in 2014. Eleven experts in primary dental care practice in Southern Brazil participated, using a three-round consensus group technique. First, the experts reached consensus on the items to be included in a 5-level diagnostic scale. They identified 21 clinical conditions and categorized them according to the oral health intervention required. Then, they described workload and activity standards for dental staff to carry out health promotion, oral disease prevention, dental treatment, dental rehabilitation, and urgent dental care. Finally, they agreed upon a set of wait times for primary dental care, establishing maximum waits from 2 to 365 days, according to the diagnostic classification. The framework demonstrated potential ability to promote more equitable access to primary dental services, since equal diagnostic classifications share the same waiting times for the dental care they require.

Evaluation of different cleaning methods for feline inhalation chambers after bacterial contamination

2020 ◽  
pp. 1098612X2091335
Author(s):  
Friederike K Klenk ◽  
Vanessa DeSimoi ◽  
Georg Wolf ◽  
Bianka S Schulz
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Respiratory Disease ◽  
Bacterial Contamination ◽  
Bacterial Culture ◽  
Boiling Water ◽  
Sample Material ◽  
Inhalation Devices ◽  
Colony Forming Units ◽  
Cleaning Methods ◽  
Lukewarm Water

Objectives Inhalation chambers are commonly used for the delivery of aerosol drugs to cats with respiratory disease. The aim of the study was to identify successful cleaning methods for inhalation devices after standardised bacterial contamination. Methods Spacer devices of two different manufacturers were used: RC Chamber (Cegla Medizintechnik) and Aerokat (Trudell Medical International). The bacterial contamination was performed using Pseudomonas aeruginosa. Previously marked areas of the chamber were contaminated with 50 μl of bacterial solution, containing between 2.2 ×105 and 2.1 ×108 colony-forming units/ml each. After cleaning the devices as recommended by each manufacturer (RC Chamber: special microwave cleaning bag [n = 5] or boiling water with liquid dish detergent for 15 mins [n = 5]; Aerokat: rinsing in a solution of lukewarm water and liquid dish detergent for 15 mins), chambers were air-dried for 24 h and samples for bacterial culture were taken from three defined areas. Sample material was applied on Müller–Hinton agar plates and subsequently incubated for 24 h at 37°C. Results Bacterial contamination was not detected in any of the examined inhalation devices using the recommended cleaning methods. Conclusions and relevance If inhalation chambers are cleaned following the manufacturers’ recommendations, successful bacterial decontamination can be expected.

scholarly journals Microbiological evaluation of bristles of frequently used toothbrushes

2012 ◽  
Vol 17 (4) ◽  
pp. 72-76 ◽  
Author(s):  
Celso André Ferreira ◽  
Geovana Dagostim Savi ◽  
Ana Paula Panatto ◽  
Jaqueline da Silva Generoso ◽  
Tatiana Barichello
Keyword(s):  
Streptococcus Pyogenes ◽  
Bacterial Contamination ◽  
Microbiological Analysis ◽  
Healthy Individuals ◽  
Macconkey Agar ◽  
Colony Forming Units ◽  
High Incidence ◽  
The World ◽  
Good Hygiene ◽  
Negative Conclusion

INTRODUCTION: Brushing teeth is probably the practice of oral hygiene most common in the world; however, inadequate use can become a risk to the population health, once they may be contaminated with various microorganisms. OBJECTIVE: The aim of this study was to evaluate the bacterial contamination on toothbrush bristles using different methodologies. METHOD: We used 40 toothbrushes from healthy individuals aged 3 to 58 years. The samples were grown in test tubes containing trypticase soy broth sterile, and with the help of a tracking 0.1 μl samples were placed on plates containing sheep blood agar 5% and MacConkey agar then the samples were stored in a bacteriological incubator at 37°C for 24 hours for later analysis. It was counted the colony forming units and bacteria identification present in the brush. RESULTS: On the microbiological analysis, there was a growth of Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes and Staphylococcus coagulase negative. CONCLUSION: According to the results presented in this study, we observed a high incidence of bacterial contamination in the brushes analyzed. The most frequent microorganisms were members of the Enterobacteriaceae. The usage time of toothbrushes may be related to contamination found and, therefore, not only good hygiene ensures the reduction of microbial load, but replacing the toothbrush can also ensure individuals better oral health.

scholarly journals Upper Lip Horizontal Line: Characteristics of a Dynamic Facial Line

2020 ◽  
Vol 17 (18) ◽  
pp. 6672
Author(s):  
Alexander D Vardimon ◽  
Nir Shpack ◽  
Atalia Wasserstein ◽  
Marilena Skyllouriotou ◽  
Morris Strauss ◽  
...  
Keyword(s):  
Dental Treatment ◽  
Age Groups ◽  
Treatment Modalities ◽  
Horizontal Line ◽  
Facial Morphology ◽  
Upper Lip ◽  
Age Related ◽  
Cast Model ◽  
And Gender ◽  
Gummy Smile

Background: Upper lip appearance received major attention with the introduction of diverse treatment modalities, including lip augmentation, rhinoplasty surgery, and dental treatment designed to support the upper lip. Our objectives were to define the prevalence and characteristics of the upper lip horizontal line (ULHL), which is a dynamic line appearing during a smile, in relation to gender, malocclusions, aging, and facial morphology. Methods: First, the prevalence and gender distribution of ULHL was examined from standardized en-face imaging at full smile of 643 randomly selected patients. Second, cephalometric and dental cast model analyses were made for 97 consecutive patients divided into three age groups. Results: ULHL appears in 13.8% of the population examined, and prevailed significantly more in females (78%). The prevalence of ULHL was not related to age nor to malocclusion. Patients presenting ULHL showed shorter upper lip and deeper lip sulcus. The skeletal pattern showed longer mid-face, shorter lower facial height and greater prevalence of a gummy smile. Conclusions: Female patients with short upper lip, concavity of the upper lip, and gummy smile are more likely to exhibit ULHL. The ULHL is not age-related and can be identified in children and young adults. Therefore, it should be considered when selecting diverse treatment modalities involving the upper lip.

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