scholarly journals Untargeted Metabolomic Profiling Reveals Variation in Metabolites Associated with Nutritional Values in Tef Accessions

2021 ◽  
Author(s):  
Aiswarya Girija ◽  
Rattan Yadav ◽  
Fiona Corke ◽  
John Doonan ◽  
Luis A. J. Mur
Keyword(s):  
Amino Acid ◽  
Untargeted Metabolomics ◽  
Eragrostis Tef ◽  
Gluten Free ◽  
Vitamin B2 ◽  
Cereal Crop ◽  
Vitamin B3 ◽  
Nutritional Values ◽  
Metabolomic Profiling ◽  
Vitamin B9

Abstract Tef (Eragrostis tef), is a gluten-free orphan cereal, crop of nutritional and economical significance. Here we used untargeted metabolomics to survey metabolite variation in 14 diverse tef accessions at 15-days post germination. Tef genotypes were classified into four metabolomic groups where variation was linked to flavones and flavonols. Further analysis on white seeded accessions shows variation related to sucrose and important vitamins, nicotinamides (vitamin B3) riboflavin (vitamin B2) and folate (vitamin B9). Coloured seeded accessions showed variation in metabolism related to amino acid and sugars. This study highlights the potential of metabolomics in exploring the nutritional traits in tef.

scholarly journals Chemical evaluation of proximate, vitamin and amino acid profile of leaf, stem bark and root of indigofera tinctoria

2020 ◽  
Vol 3 (10) ◽  
pp. 150-157
Author(s):  
Alagbe, J.O
Keyword(s):  
Amino Acid ◽  
Vitamin B1 ◽  
Stem Bark ◽  
Amino Acid Profile ◽  
Vitamin B3 ◽  
Crude Fibre ◽  
Chemical Evaluation ◽  
Vitamin B9 ◽  
Therapeutic Value ◽  
Indigofera Tinctoria

Medicinal plants contain substances with high therapeutic value because they contain multiple bioactive chemicals. Chemical analysis of Indigofera tinctoria leaves, stem bark and roots was evaluated. The result revealed that proximate composition of Indigofera tinctoria leaves contained 12.49 % moisture content (M.C), 87.51 % dry matter (DM), 30.53 % crude protein (CP), 19.02 % crude fibre (CF), 2.44 % ether extract (EE), 13.86 % ash, 36.59 % carbohydrate (CHO) and 254.1 kcal/100g energy (ME). Indigofera tinctoria stem bark contained M.C (6.40 %), DM (93.60 %), CP (5.11 %), CF (54.49 %), EE (2.00 %), ash (10.42 %), CHO (29.98 %) and ME (156.0 kcal/100g). Indigofera tinctoria roots contained MC, DM, CP, CF, EE, ash, CHO and ME at 10.04 %, 89.60 %, 8.22 %, 40.88 %, 1.21 %, 8.43 %, 42.47 % and 210.0 kcal/100g respectively. Vitamin analysis showed that Indigofera tinctoria leaves, stem bark and roots contained β- carotene (8.45, 2.88 and 5.11 mg/100 g), Vitamin B1 (1.94, 0.33 and 1.00 mg/100 g), Vitamin B2 (0.71, 0.21 and 0.50 mg/100 g), Vitamin B3 (0.66, 0.34 and 0.48 mg/100 g), Vitamin B6 (0.32, 0.21 and 0.30 mg/100 g), Vitamin B7 (0.63, 0.01 and 0.16 mg/100 g), Vitamin B9 (0.26, 0.10 and 0.18 mg/100 g), Vitamin B12 (0.21, 0.03 and 0.10 mg/100 g), Vitamin C (14.0, 3.56 and 9.44 mg/100 g), Vitamin D (0.10, 0.01 and 0.06 mg/100 g) and Vitamin K (0.17, 0.07 and 0.12 mg/100 g). Amino acid analysis revealed the presence of threonine, leucine, lysine, valine, tryptophan, glycine, phenylalanine, histidine, methionine, alanine, serine, proline, aspartate, glutamic acid, tryrosine and cysteine in Indigofera tinctoria leaves, stem bark and roots at (7.65 %, 1.22 % and 3.03 %), (5.76, 1.09 % and 2.46 %), (3.11 %, 1.21% and 2.00 %), (7.21 %, 3.53 % and 4.09 %), (1.45%, 0.03% and 1.00 %), (4.76 %, 0.08 % and 2.33 %), (6.33 %, 2.45 % and 3.49 %), (7.42 %, 2.00 % and 3.00 %), (3.49 %, 0.01 % and 2.00 %), (2.41 %, 0.56 % and 1.20 %), (5.23 %, 1.22 % and 1.76 %), (2.87 %, 0.57 % and 1.00 %), (5.32 %, 2.11 % and 3.56 %), (9.66 %, 4.21 % and 5.11 %), (2.45 %, 0.57 % and 1.67 %) and (1.85 %, 0.81 % and 0.89 %) respectively. It was concluded that Indigofera tinctoria leaves, stem bark and roots are loaded with significant quantity of nutrients, vitamins and amino acid (leaves ˃ roots ˃ stem bark).

scholarly journals Chemical Evaluation of Proximate, Vitamin and Amino Acid Profile of Leaf, Stem Bark and Root of Indigofera Tinctoria

2021 ◽  
Vol 3 (1) ◽  
pp. 01-06
Author(s):  
Alagbe J.O
Keyword(s):  
Amino Acid ◽  
Vitamin B1 ◽  
Stem Bark ◽  
Amino Acid Profile ◽  
Vitamin B3 ◽  
Crude Fibre ◽  
Chemical Evaluation ◽  
Vitamin B9 ◽  
Therapeutic Value ◽  
Indigofera Tinctoria

Medicinal plants contain substances with high therapeutic value because they contain multiple bioactive chemicals. Chemical analysis of Indigofera tinctoria leaves, stem bark and roots was evaluated. The result revealed that proximate composition of Indigofera tinctoria leaves contained 12.49 % moisture content (M.C), 87.51 % dry matter (DM), 30.53 % crude protein (CP), 19.02 % crude fibre (CF), 2.44 % ether extract (EE), 13.86 % ash, 36.59 % carbohydrate (CHO) and 254.1 kcal/100g energy (ME). Indigofera tinctoria stem bark contained M.C (6.40 %), DM (93.60 %), CP (5.11 %), CF (54.49 %), EE (2.00 %), ash (10.42 %), CHO (29.98 %) and ME (156.0 kcal/100g). Indigofera tinctoria roots contained MC, DM, CP, CF, EE, ash, CHO and ME at 10.04 %, 89.60 %, 8.22 %, 40.88 %, 1.21 %, 8.43 %, 42.47 % and 210.0 kcal/100g respectively. Vitamin analysis showed that Indigofera tinctoria leaves, stem bark and roots contained β- carotene (8.45, 2.88 and 5.11 mg/100 g), Vitamin B1 (1.94, 0.33 and 1.00 mg/100 g), Vitamin B2 (0.71, 0.21 and 0.50 mg/100 g), Vitamin B3 (0.66, 0.34 and 0.48 mg/100 g), Vitamin B6 (0.32, 0.21 and 0.30 mg/100 g), Vitamin B7 (0.63, 0.01 and 0.16 mg/100 g), Vitamin B9 (0.26, 0.10 and 0.18 mg/100 g), Vitamin B12 (0.21, 0.03 and 0.10 mg/100 g), Vitamin C (14.0, 3.56 and 9.44 mg/100 g), Vitamin D (0.10, 0.01 and 0.06 mg/100 g) and Vitamin K (0.17, 0.07 and 0.12 mg/100 g). Amino acid analysis revealed the presence of threonine, leucine, lysine, valine, tryptophan, glycine, phenylalanine, histidine, methionine, alanine, serine, proline, aspartate, glutamic acid, tryrosine and cysteine in Indigofera tinctoria leaves, stem bark and roots at (7.65 %, 1.22 % and 3.03 %), (5.76, 1.09 % and 2.46 %), (3.11 %, 1.21% and 2.00 %), (7.21 %, 3.53 % and 4.09 %), (1.45%, 0.03% and 1.00 %), (4.76 %, 0.08 % and 2.33 %), (6.33 %, 2.45 % and 3.49 %), (7.42 %, 2.00 % and 3.00 %), (3.49 %, 0.01 % and 2.00 %), (2.41 %, 0.56 % and 1.20 %), (5.23 %, 1.22 % and 1.76 %), (2.87 %, 0.57 % and 1.00 %), (5.32 %, 2.11 % and 3.56 %), (9.66 %, 4.21 % and 5.11 %), (2.45 %, 0.57 % and 1.67 %) and (1.85 %, 0.81 % and 0.89 %) respectively. It was concluded that Indigofera tinctoria leaves, stem bark and roots are loaded with significant quantity of nutrients, vitamins and amino acid (leaves ˃ roots ˃ stem bark).

scholarly journals Evaluation of Amino Acid and Vitamin B2 and E Composition of Genetically Modified Antidwarf Mosaic Maize by Automatic Amino acid Analyzer and HPLC

2013 ◽  
Vol 25 (6) ◽  
pp. 3525-3526 ◽  
Author(s):  
Pingmei Yan ◽  
Qing Wang ◽  
Xiaoyan Yan ◽  
Xiao-Hui Chang ◽  
Jian-Zhong Du ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genetically Modified ◽  
Vitamin B2 ◽  
Automatic Amino Acid Analyzer ◽  
Amino Acid Analyzer

scholarly journals Citrus polymethoxyflavones attenuate metabolic syndrome by regulating gut microbiome and amino acid metabolism

2020 ◽  
Vol 6 (1) ◽  
pp. eaax6208 ◽  
Author(s):  
Su-Ling Zeng ◽  
Shang-Zhen Li ◽  
Ping-Ting Xiao ◽  
Yuan-Yuan Cai ◽  
Chu Chu ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Amino Acid ◽  
Gut Microbiota ◽  
Metabolic Diseases ◽  
Amplicon Sequencing ◽  
Therapeutic Interventions ◽  
Protective Effects ◽  
Fecal Microbiome ◽  
Metabolomic Profiling ◽  
Branched Chain

Metabolic syndrome (MetS) is intricately linked to dysregulation of gut microbiota and host metabolomes. Here, we first find that a purified citrus polymethoxyflavone-rich extract (PMFE) potently ameliorates high-fat diet (HFD)–induced MetS, alleviates gut dysbiosis, and regulates branched-chain amino acid (BCAA) metabolism using 16S rDNA amplicon sequencing and metabolomic profiling. The metabolic protective effects of PMFE are gut microbiota dependent, as demonstrated by antibiotic treatment and fecal microbiome transplantation (FMT). The modulation of gut microbiota altered BCAA levels in the host serum and feces, which were significantly associated with metabolic features and actively responsive to therapeutic interventions with PMFE. Notably, PMFE greatly enriched the commensal bacterium Bacteroides ovatus, and gavage with B. ovatus reduced BCAA concentrations and alleviated MetS in HFD mice. PMFE may be used as a prebiotic agent to attenuate MetS, and target-specific microbial species may have unique therapeutic promise for metabolic diseases.

Chemical Composition and Nutritive Value of Protein of the Pea Seeds - Effect of Harvesting Year and Variety

2011 ◽  
Vol 75 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Anna Kotlarz ◽  
Agnieszka Sujak ◽  
Wacław Strobel ◽  
Wilhelm Grzesiak
Keyword(s):  
Amino Acid ◽  
Chemical Composition ◽  
Crude Oil ◽  
Crude Protein ◽  
Nutritive Value ◽  
Amino Acid Content ◽  
Crude Fibre ◽  
Nutritional Values ◽  
Fiber Fractions ◽  
Pea Seeds

Chemical Composition and Nutritive Value of Protein of the Pea Seeds - Effect of Harvesting Year and VarietyWe tested nutritional usability of pea seeds (Pisum sativumL.) at full maturity. Four white-flowering and 5 colour-flowering new Polish cultivars were compared. We determined proximate composition of ground seeds collected over 4 years period as well as amino acids, minerals (Ca, P, Na, K), tannins and fiber fractions (NDF, ADF, ADL). The seeds contained between 224 and 260 g·kg-1of crude protein which was rich in Lys (6.8±0.8 g) but poor in Met+Cys (2.0±0.2 g·16 g-1N). The amount of tannins (as tannic acid equivalent) in white-flowering cvs was 4.3±0.9 g and in colour-flowering - 7.4±2.2 g. The calculated protein nutritional values were compared against amino acid standards of human and animal nutrition and the whole egg protein. Effects of the cropping year and variety variance in respect of chemical composition of seeds were examined. Crude protein, crude oil, N-free extractives, tannins, K, amino acid content: Leu, Phe+Tyr and Ala content were influenced significantly by the cultivar, while the cropping year had a significant influence on dry matter, crude ash, crude protein, crude fibre, crude oil, N-free extractives ADF and ADL fiber fractions, content of P, Na and K, most of the amino acid levels and on nutritional values of the protein measured by means of CS and EAAI indices.

scholarly journals Glucagon Receptor Signaling and Glucagon Resistance

2019 ◽  
Vol 20 (13) ◽  
pp. 3314 ◽  
Author(s):  
Janah ◽  
Kjeldsen ◽  
Galsgaard ◽  
Winther-Sørensen ◽  
Stojanovska ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Metabolic Diseases ◽  
Physiological Role ◽  
Glucagon Receptor ◽  
Metabolomic Profiling ◽  
Hepatic Fat

Hundred years after the discovery of glucagon, its biology remains enigmatic. Accurate measurement of glucagon has been essential for uncovering its pathological hypersecretion that underlies various metabolic diseases including not only diabetes and liver diseases but also cancers (glucagonomas). The suggested key role of glucagon in the development of diabetes has been termed the bihormonal hypothesis. However, studying tissue-specific knockout of the glucagon receptor has revealed that the physiological role of glucagon may extend beyond blood-glucose regulation. Decades ago, animal and human studies reported an important role of glucagon in amino acid metabolism through ureagenesis. Using modern technologies such as metabolomic profiling, knowledge about the effects of glucagon on amino acid metabolism has been expanded and the mechanisms involved further delineated. Glucagon receptor antagonists have indirectly put focus on glucagon’s potential role in lipid metabolism, as individuals treated with these antagonists showed dyslipidemia and increased hepatic fat. One emerging field in glucagon biology now seems to include the concept of hepatic glucagon resistance. Here, we discuss the roles of glucagon in glucose homeostasis, amino acid metabolism, and lipid metabolism and present speculations on the molecular pathways causing and associating with postulated hepatic glucagon resistance.

Biochemical and technological characterization of two C 4 gluten‐free cereals: Sorghum bicolor and Eragrostis tef

2019 ◽  
Vol 97 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Elena Galassi ◽  
Federica Taddei ◽  
Roberto Ciccoritti ◽  
Francesca Nocente ◽  
Laura Gazza
Keyword(s):  
Sorghum Bicolor ◽  
Eragrostis Tef ◽  
Gluten Free

Effect of Teff (Eragrostis tef) on Chemical and Technological Quality of Gluten-free Breads

2019 ◽  
Vol 18 (6) ◽  
pp. 535-548 ◽  
Author(s):  
Raisa Vieira Homem ◽  
Aline dos Santos Joaquim ◽  
Henrique Pimentel da Silva ◽  
Sabrina Mello Evangelista ◽  
Marina Rocha Komeroski ◽  
...  
Keyword(s):  
Eragrostis Tef ◽  
Gluten Free ◽  
Technological Quality

scholarly journals Effect of Egg White Protein and Soy Protein Isolate Addition on Nutritional Properties and In-Vitro Digestibility of Gluten-Free Pasta Based on Banana Flour

Foods ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 589 ◽  
Author(s):  
Adetiya Rachman ◽  
Margaret A. Brennan ◽  
James Morton ◽  
Charles S. Brennan
Keyword(s):  
Amino Acid ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Protein Digestibility ◽  
Egg White ◽  
Protein Isolate ◽  
Gluten Free ◽  
Egg White Protein ◽  
Amino Acid Profiles ◽  
Banana Flour

The effects of egg white protein and soy protein isolate addition on the nutritional and digestibility of gluten-free pasta based on banana flour were studied. The level of protein additions (soy protein or egg white protein) were 0, 5, 10 and 15% of banana flour (w/w). Pasta made from 100% durum wheat semolina was used as a control. Soy protein isolate inclusion into banana pasta increased total phenolic content (TPC) and antioxidant capacities, while egg white protein decreased the TPC and antioxidant capacities with the increasing level of addition. Starch digestibility was affected by the type of protein addition. Egg white protein lowered starch digestibility compared to soy protein isolate. Protein inclusion in banana pasta also altered protein digestibility, amino acid profiles and protein digestibility-corrected amino acid score (PDCAAS). Soy protein isolate increased protein digestibility of gluten-free pasta compared to egg white protein. Protein enrichment gave better amino acid profiles of banana pasta compared to semolina pasta with egg white protein and performed a better PDCAAS compared to soy protein isolate. These results showed that soy protein isolate and egg white protein addition enhanced nutritional qualities and digestibility properties of gluten-free banana pasta.

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