scholarly journals Effects of growth conditions and cultivability on the content of cyclitols in Medicago sativa

2020 ◽  
Author(s):  
I. A. Ratiu ◽  
H. Al-Suod ◽  
M. Ligor ◽  
F. Monedeiro ◽  
B. Buszewski
Keyword(s):  
Secondary Metabolites ◽  
Medicago Sativa ◽  
Abiotic Factors ◽  
Growth Conditions ◽  
Alfalfa Plant ◽  
Naturally Occurring ◽  
Bioactive Properties ◽  
Seasonal Impact ◽  
Low Humidity ◽  
Plant Treatment

Abstract Cyclitols are secondary metabolites with bioactive properties, naturally occurring in plants. Accumulation of such metabolites is directly connected with abiotic stressing factors. This article investigates the influence exercised by a series of abiotic factors including plant treatment with elicitors and cultivability in various regions of Europe, in some cases during two seasons, onto the amounts of cyclitols produced in alfalfa (Medicago sativa L.). The obtained results highlighted that NaCl elicitation acts to increase the quantity of cyclitols, while AgNO3 and Zn(NO3)2 generally decreased the obtained amount of cyclitols. When considering the seasonal impact, samples harvested in August presented a double amount of cyclitols in comparison with those collected in May. Correlation analysis proved that this phenomenon is related mainly to sunshine period versus low humidity. However, our investigation suggests that soil type, salinity level, lack of humidity, the number of sunny days, and plant elicitation play a role on the amount of cyclitols produced in alfalfa plant.

Screening for naturally occurring oxyprenylated secondary metabolites as cancer cell growth inhibitory agents

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
F Epifano ◽  
S Genovese ◽  
P Lullo ◽  
S Fiorito ◽  
G Trivisonno ◽  
...  
Keyword(s):  
Cell Growth ◽  
Secondary Metabolites ◽  
Cancer Cell ◽  
Cancer Cell Growth ◽  
Naturally Occurring ◽  
Growth Inhibitory ◽  
Inhibitory Agents

Assimilation of various organic carbon sources by Haematococcus strains

2009 ◽  
Vol 57 (2) ◽  
pp. 231-237
Author(s):  
M. Zych ◽  
A. Stolarczyk ◽  
K. Maca ◽  
A. Banaś ◽  
K. Termińska-Pabis ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Organic Compounds ◽  
Present Experiment ◽  
Carbon Sources ◽  
Growth Conditions ◽  
Mixotrophic Growth ◽  
Naturally Occurring ◽  
Organic Carbon Sources ◽  
Secondary Carotenoids ◽  
Colour Changes

Differences in the assimilation of individual organic compounds (5 mM sugars and L-asparagine) under mixotrophic growth conditions were described for three naturally occurring Haematococcus strains.The effects of assimilation were measured by the growth intensity and size of algal cells, and the effect of colour changes in the cultures was observed. Some compounds caused the cell colouration to change from green to yellow, being the result of chlorophyll disappearance and the accumulation of yellow secondary carotenoids. In the present experiment none of the cultures turned red, thus excluding the intense accumulation of the commercially interesting carotenoid, astaxanthin.

scholarly journals Naturally Occurring Ecdysteroids in Triticum aestivum L. and Evaluation of Fenarimol as a Potential Inhibitor of Their Biosynthesis in Plants

2021 ◽  
Vol 22 (6) ◽  
pp. 2855
Author(s):  
Anna Janeczko ◽  
Jana Oklestkova ◽  
Danuše Tarkowská ◽  
Barbara Drygaś
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Triticum Aestivum L ◽  
Growth Conditions ◽  
Animal Kingdom ◽  
Experimental Conditions ◽  
P450 Monooxygenase ◽  
Naturally Occurring ◽  
Regulatory Effects ◽  
Potential Use

Ecdysteroids (ECs) are steroid hormones originally found in the animal kingdom where they function as insect molting hormones. Interestingly, a relatively high number of these substances can also be formed in plant cells. Moreover, ECs have certain regulatory effects on plant physiology, but their role in plants still requires further study. One of the main aims of the present study was to verify a hypothesis that fenarimol, an inhibitor of the biosynthesis of ECs in the animal kingdom, also affects the content of endogenous ECs in plants using winter wheat Triticum aestivum L. as a model plant. The levels of endogenous ECs in winter wheat, including the estimation of their changes during a course of different temperature treatments, have been determined using a sensitive analytical method based on UHPLC-MS/MS. Under our experimental conditions, four substances of EC character were detected in the tissue of interest in amounts ranging from less than 1 to over 200 pg·g−1 FW: 20-hydroxyecdysone, polypodine B, turkesterone, and isovitexirone. Among them, turkesterone was observed to be the most abundant EC and accumulated mainly in the crowns and leaves of wheat. Importantly, the level of ECs was observed to be dependent on the age of the plants, as well as on growth conditions (especially temperature). Fenarimol, an inhibitor of a cytochrome P450 monooxygenase, was shown to significantly decrease the level of naturally occurring ECs in experimental plants, which may indicate its potential use in studies related to the biosynthesis and physiological function of these substances in plants.

scholarly journals Soil microbiomes mediate degradation of vinyl ester-based polymer composites

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Adam M. Breister ◽  
Muhammad A. Imam ◽  
Zhichao Zhou ◽  
Md Ariful Ahsan ◽  
Juan C. Noveron ◽  
...  
Keyword(s):  
Polymer Composites ◽  
Polymer Composite ◽  
Structural Integrity ◽  
Abiotic Factors ◽  
High Strength ◽  
Strength Properties ◽  
Naturally Occurring ◽  
Structural Applications ◽  
Acrylate Esters ◽  
Bacterial Groups

AbstractPolymer composites are attractive for structural applications in the built environment due to their lightweight and high strength properties but suffer from degradation due to environmental factors. While abiotic factors like temperature, moisture, and ultraviolet light are well studied, little is known about the impacts of naturally occurring microbial communities on their structural integrity. Here we apply complementary time-series multi-omics of biofilms growing on polymer composites and materials characterization to elucidate the processes driving their degradation. We measured a reduction in mechanical properties due to biologically driven molecular chain breakage of esters and reconstructed 121 microbial genomes to describe microbial diversity and pathways associated with polymer composite degradation. The polymer composite microbiome is dominated by four bacterial groups including the Candidate Phyla Radiation that possess pathways for breakdown of acrylate, esters, and bisphenol, abundant in composites. We provide a foundation for understanding interactions of next-generation structural materials with their natural environment that can predict their durability and drive future designs.

scholarly journals Influence of stress conditions on the quality of obtained sprouts – modification of their chemical composition

2021 ◽  
Vol 13 (2) ◽  
pp. 1-12
Author(s):  
Magdalena Zielińska-Dawidziak
Keyword(s):  
Chemical Composition ◽  
Secondary Metabolites ◽  
Metal Ions ◽  
Nutritional Quality ◽  
Growth Conditions ◽  
Stress Conditions ◽  
Light Illumination ◽  
Health Promoting ◽  
Chemical Factors

Sprouts are generally accepted as a pro-healthy food. They are consumed as a source of valuable macronutrients, antioxidants, microelements, and vitamins. Changing growth conditions of sprouts enables modification of their nutritional quality, as well as their safety. Thus, in order to achieve the most desirable composition of the produced sprouts, the conditions for their production are optimized. The aim of this review is to present methods currently used to modify the nutritional quality of plant sprouts. Most scientific works focus on stress conditions inducing the synthesis of secondary metabolites, mainly antioxidants. An increase in their content is achieved after application of physical (e.g., light illumination, temperature) or chemical factors (e.g., salinity stress, phytohormones, metal ions, etc). Though the application of these modifications on a larger scale is problematic. These problems include difficulties in predicting the effect of the stressor and an increased price of the obtained sprouts. However, since it is possible to enrich sprouts with valuable health-promoting substances, these methods are still considered very promising.

scholarly journals Urgensi dan Mekanisme Biosintesis Metabolit Sekunder Mikroba Laut

2012 ◽  
Vol 10 (2) ◽  
pp. 120 ◽  
Author(s):  
Risa Nofiani
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Metabolic Pathways ◽  
Abiotic Factors ◽  
Biologically Active ◽  
Secondary Metabolite Production ◽  
Marine Microorganism ◽  
Metabolite Production ◽  
Biotic And Abiotic Factors ◽  
Living Materials

Marine microorganism is one of biologically active potential resources of secondary metabolites. Its potency areso promising that the knowledge of how its secondary metabolite occured need to be studied and collected. Thoseknowledges will enable further study is improving secondary metabolite production in the laboratory. In nature,secondary metabolites synthesis occur when there are effect of both biotic and abiotic factors such as sea waterand microbe symbiosis with other living materials. When this is explained in metabolic pathways, secondarymetabolite synthesis affected by available nutrient and regulated by autoinducer molecules through quorum sensingmechanism

scholarly journals SEASONAL VARIATION OF PHENOLS AND FLAVONOIDS IN NON-GALLED AND GALLED LEAVES OF CARYOCAR BRASILIENSE (CARYOCARACEAE)

2021 ◽  
Vol 18 (38) ◽  
pp. 38-46
Author(s):  
Edson Cardoso TEIXEIRA ◽  
Rafaela Oliveira FERREIRA
Keyword(s):  
Secondary Metabolites ◽  
Abiotic Factors ◽  
Complexation Reaction ◽  
Strong Positive Correlation ◽  
Total Phenols ◽  
Leaf Extracts ◽  
Strong Negative Correlation ◽  
Caryocar Brasiliense ◽  
Maximum Temperatures ◽  
Galling Insects

Introduction: Galling insects manipulate the development of their host plants. Galls are rich in secondary metabolites, such as phenolic compounds and terpenes. Caryocar brasiliense acts as a host of galling insects. Few studies report the impacts of this plant-insect interaction on the species' morphology, physiology, and secondary metabolism. Objective: The purpose of this study was to verify if there is a correlation between the levels of total phenols and flavonoids in C. brasiliense leaf extracts and environmental variables, such as temperature and precipitation, throughout the year, and to evaluate how galling insects can interfere in this seasonality. Methods: Ethanol extracts from leaves were obtained by soxhlet extraction. The main classes of secondary metabolites were detected by phytochemical prospecting. The levels of total phenols and flavonoids were quantified by the Folin-Dennis tests and complexation reaction with aluminum chloride, respectively. Pearson 's correlation coefficient shows that the levels of phenols and flavonoids were correlated with the average monthly maximum temperatures and precipitation accumulation, respectively, recorded in Gurupi - Tocantins. Results and Discussion: Phytochemical prospecting indicated flavonoids, saponins, tannins, triterpenes, steroids, and alkaloids in both extracts. In C. brasiliense, the samples of healthy leaves suffered a greater variation in the levels of phenols (68.39±2.3 to 279.34±3.1 mg GAE g-1 extract) and flavonoids (164.06± 3.0 at 269.03±3.6 mg QE g-1 extract) compared to leaf samples with galls. A strong positive correlation was found between the contents of total phenols and maximum temperatures and a strong negative correlation with precipitation through Pearson's correlation. Conclusions: The variations observed in the levels of phenols and flavonoids in the extracts of C. brasiliense seem to be related to abiotic factors and the herbivory promoted by the galling insect.

scholarly journals Bioactive Compounds from Brassicaceae: a Beneficial Contribution in our Everyday Diet

2011 ◽  
Vol 68 (2) ◽  
Author(s):  
Renato IORI ◽  
Gina R. DE NICOLA ◽  
Manuela BAGATTA ◽  
Eleonora PAGNOTTA
Keyword(s):  
Secondary Metabolites ◽  
Dietary Intake ◽  
Bioactive Compounds ◽  
Chemical Structure ◽  
Side Chain ◽  
Variable Part ◽  
Naturally Occurring ◽  
Interesting Class

Dietary intake of Brassicaceae (Crucifers) provides not only nutrients, but also a highly interesting class of secondary metabolites beneficial to health, known as glucosinolates (GLs). These compounds possess a -D-glucopyranosyl unit connected to a O-sulfated anomeric Z-thiohydroximate function, and a side chain R which is the only variable part of the chemical structure. Up to now, more than 120 naturally-occurring GLs have been carachterized

scholarly journals Influence of soluble content losses on the ability of aerobic fungi to improve the nutritional content of pre-treated forages

2020 ◽  
Vol 47 (3) ◽  
pp. 298-308
Author(s):  
K. R. Idowu ◽  
A. S. Chaudhry ◽  
J. Dolfing ◽  
V. O. A. Ojo
Keyword(s):  
Secondary Metabolites ◽  
Growth Conditions ◽  
Water Soluble ◽  
Optimal Conditions ◽  
Similar Reduction ◽  
Filtering Method ◽  
Nutritive Quality ◽  
Andropogon Gayanus ◽  
The Impact

Fungal improvement of the nutritive content of low-quality forages can be affected by several factors among which loss of water-soluble content (WSC) plays a major role. To achieve this aim, two growth conditions i.e. forage-liquid ratios (1:3 and 1:5) and two inoculation times (14 and 28 days) were used to cultivate the selected fungi i.e. Pleurotus ostreatus (PO) at 30°C and Ceriporiopsis rivulosus (CR) at 20°C on Brachiaria decumbens (BD), Andropogon gayanus (AG), Triticum aestivum (TA) straw, Lolium perenne (LP), respectively with the view of selecting the optimal conditions that facilitate the release of WSC. The impact that losses of WSC have on the ability of fungi to improve the nutritive content (i.e. proximate, fibre, secondary metabolites and total antioxidant content) of the forages were then measured using 2 filtering methods i.e. light pump filtering method (LFM) and free flow filtering method (FFM). The optimal conditions that supported increased th soluble was identified as 28th day for both fungi; forage-liquid ratio of 1:5 for both fungi in most of the forages except BD (CR) and BD & AG (PO). The LFM led to lesser or no improvement in the nutritive quality of the upgraded forages while the opposite was recorded with the LFM. The LFM as against the FFM produced upgraded forages with lesser reduction in NDF; similar or higher ADF and lignin contents; and similar reduction in secondary metabolites and TAC when compared with un-improved forages. It can be concluded that the fungal improvement of the nutritive content of low-quality forages was negatively affected by the loss of WSC. It is therefore recommended that fungal improvement of the nutritive quality of low quality forages should be carried out with methods or techniques that facilitates little or no WSC loss in the upgraded forages.

scholarly journals Ecometabolomics for a Better Understanding of Plant Responses and Acclimation to Abiotic Factors Linked to Global Change

Metabolites ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 239 ◽  
Author(s):  
Jordi Sardans ◽  
Albert Gargallo-Garriga ◽  
Otmar Urban ◽  
Karel Klem ◽  
Tom W.N. Walker ◽  
...  
Keyword(s):  
Amino Acids ◽  
Heat Stress ◽  
Global Change ◽  
Secondary Metabolites ◽  
De Novo ◽  
Abiotic Factors ◽  
Soluble Sugars ◽  
Metabolic Responses ◽  
Plant Responses ◽  
Co2 Concentrations

The number of ecometabolomic studies, which use metabolomic analyses to disentangle organisms’ metabolic responses and acclimation to a changing environment, has grown exponentially in recent years. Here, we review the results and conclusions of ecometabolomic studies on the impacts of four main drivers of global change (increasing frequencies of drought episodes, heat stress, increasing atmospheric carbon dioxide (CO2) concentrations and increasing nitrogen (N) loads) on plant metabolism. Ecometabolomic studies of drought effects confirmed findings of previous target studies, in which most changes in metabolism are characterized by increased concentrations of soluble sugars and carbohydrate derivatives and frequently also by elevated concentrations of free amino acids. Secondary metabolites, especially flavonoids and terpenes, also commonly exhibited increased concentrations when drought intensified. Under heat and increasing N loads, soluble amino acids derived from glutamate and glutamine were the most responsive metabolites. Foliar metabolic responses to elevated atmospheric CO2 concentrations were dominated by greater production of monosaccharides and associated synthesis of secondary metabolites, such as terpenes, rather than secondary metabolites synthesized along longer sugar pathways involving N-rich precursor molecules, such as those formed from cyclic amino acids and along the shikimate pathway. We suggest that breeding for crop genotypes tolerant to drought and heat stress should be based on their capacity to increase the concentrations of C-rich compounds more than the concentrations of smaller N-rich molecules, such as amino acids. This could facilitate rapid and efficient stress response by reducing protein catabolism without compromising enzymatic capacity or increasing the requirement for re-transcription and de novo biosynthesis of proteins.

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