Chemical composition, antioxidant capacity, toxicity and antibacterial activity of the essential oils from Acantholippia deserticola (Phil.) Moldenke (Rica rica) and Artemisia copa Phil. (Copa copa) extracted by microwave-assisted hydrodistillation

2019 ◽  
Vol 142 ◽  
pp. 111830 ◽  
Author(s):  
María Larrazabal-Fuentes ◽  
Jenifer Palma ◽  
Adrián Paredes ◽  
Ana Mercado ◽  
Iván Neira ◽  
...  
2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.


2020 ◽  
Vol 10 (3) ◽  
pp. 272-278
Author(s):  
Ardalan Pasdaran ◽  
Satyajit D. Sarker ◽  
Lutfun Nahar ◽  
Azadeh Hamedi

Background: The essential oil from the Acantholimon genus have been an integral part of the traditional food additive in Middle East. Most of the plants in Acantholimon genus have not been studied scientifically. The aim of this study is to investigate the chemical composition, antibacterial, insecticidal and anti-oxidant activities of three Acantholimon species including Acantholimon atropatanum, A. gilliatii and A. tragacanthium. Method: The essential oils of the aerial parts were extracted by hydrodistillation. Chemical constitutions were identified by gas chromatography- mass spectroscopy technique, also their toxicities were assessed against the two important grain products pests, Oryzeaphilus mercator and Tribolium castaneum. Antibacterial activity was assessed against the three foodborne bacteria that include Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus based on the disc diffusion assay. Free-radical-scavenging property was identified based on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Results: 2-hexahydrofarnesyl acetone was the main compound in A. gilliatii and A. tragacanthium, whilst farnesyl acetone, heptacosane and germacrene D were the principal components of A. atropatanum essential oil. These oils exhibited 40-90% mortality of O. mercator and/or T. castaneum at a dose of 12 μl/l air after 48h of exposure, and exhibited significant free-radicalscavenging property (RC50 = 3.7 × 10-3 - 8.3 × 10-3 mg/ml). The oils of A. tragacanthium and A. gilliatii showed a weaker antibacterial activity compared to A. atropatanum. Conclusion: A. atropatanum, A. gilliatii and A. tragacanthium essential oils had significant insecticidal and anti-oxidant properties. They also showed week to moderate antibacterial activity against P. aeruginosa and S. aureus.


2020 ◽  
Vol 71 (6) ◽  
pp. 267-273 ◽  
Author(s):  
Branislava D. Kocic ◽  
Dobrila M. Stankovic Dordevic ◽  
Marija V. Dimitrijevic ◽  
Marija S. Markovic ◽  
Dragoljub L. Miladinovic

The susceptibility of Helicobacter pylori to three essential oils (EOs), 12 naturally occurring monoterpene hydrocarbons, oxygenated and phenolic monoterpenes and three reference antibiotics were studied. Classification and comparison of essential oils and monoterpenes on the basis of their chemical composition and antibacterial activity were made by the utilization of principal component analyses (PCA) and agglomerative hierarchical clustering (AHC). The most abundant compound in the Thymus glabrescens Willd. and Thymus pulegioides L. EOs is geraniol (33.8% and 52.5%), while the main constituent in Satureja kitaibelii Wierzb. ex Heuff. EO is limonene (16,1%). The compound that was the most active against H. pylori was carvacrol. EOs of T. glabrescens and S. kitaibelii exhibit higher antibacterial ability in comparison with all monoterpenes, except carvacrol, probably based on the concept of synergistic activity of essential oil components. PCA separated essential oils based on chemical composition and explain 96.5% of the total variance in the first two principal components. Essential oils, phenolic monoterpenes and two antibiotics were classified in the same sub-cluster within AHC analyses. EOs of T. glabrescens and S. kitaibelii can be used to treat infections caused by H. pylori, as a potentially effective, cheap and safe natural products. Further research of antibacterial activity of selected monoterpenes, essential oils and standard antibiotic combinations, as well as clinical study are required.


2014 ◽  
Vol 04 (04) ◽  
pp. 246-252 ◽  
Author(s):  
Lateef S. Kasim ◽  
Kafayat O. Olaleye ◽  
A. B. Fagbohun ◽  
S. F. Ibitoye ◽  
O. E. Adejumo

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5848
Author(s):  
Oberdan Oliveira Ferreira ◽  
Celeste de Jesus Pereira Franco ◽  
Everton Luiz Pompeu Varela ◽  
Sebastião Gomes Silva ◽  
Márcia Moraes Cascaes ◽  
...  

Eugenia florida DC. belongs to the Myrtaceae family, which is present in almost all of Brazil. This species is popularly known as pitanga-preta or guamirim and is used in folk medicine to treat gastrointestinal problems. In this study, two specimens of Eugenia florida (Efl) were collected in different areas of the same region. Specimen A (EflA) was collected in an area of secondary forest (capoeira), while specimen B (EflB) was collected in a floodplain area. The essential oils (EOs) were extracted from both specimens of Eugenia florida by means of hydrodistillation. Gas chromatography coupled to mass spectrometry (GC/MS) was used to identify the volatile compounds present, and the antioxidant capacity of the EOs was determined by antioxidant capacity (AC-DPPH) and the Trolox equivalent antioxidant (TEAC) assay. For E. florida, limonene (11.98%), spathulenol (10.94%) and α-pinene (5.21%) were identified as the main compounds of the EO extracted from sample A, while sample B comprised selina-3,11-dien-6α-ol (12.03%), eremoligenol (11.0%) and γ-elemene (10.70%). This difference in chemical composition impacted the antioxidant activity of the EOs between the studied samples, especially in sample B of E. florida. This study is the first to report on the antioxidant activity of Eugenia florida DC. essential oils.


Author(s):  
Fatima El Kamari ◽  
Amal Taroq ◽  
Yassine El Atki ◽  
Imane Aouam ◽  
Badiaa Lyoussi ◽  
...  

Objective: The aim of the current study is to determine the chemical composition and evaluate antibacterial activity of Vitex agnus-castus L. (VAC) essential oils against some bacteria causing nosocomial infections in the neonatal and intensive care rooms at the university hospital center of Fez Morocco. Methods: The phytochemical screening of essential oils was determined using gas chromatography (GC) and GC-mass spectrometry analysis. The antibacterial test was evaluated against Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria species (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis) using disc diffusion method. Results: Twenty-nine components were identified in the fruits’ oil representing 93.1% of total oil. The major components in the fruits oil are 1,8-cineole (11.6%), α-thujene (9.3%), phyllocladene (8.2%), α-pinene (7.9%), caryophyllene (5.9%), and cubenol (5%). Furthermore, 28 components were identified in the leaf essential oil. The main component was caryophyllene (9.5%), followed by 1,8-cineole (8.7%), manoyl oxide (7.3%), eugenyl acetate (7.1%), phyllocladene (6.8%), and α-pinene (5.2%). Antibacterial activity of both oils showed a strong activity against nosocomial bacteria tested. Conclusion: Essential oils of Moroccan VAC could be exploited as natural drugs for bacteria, especially those who have acquired resistance to conventional antibiotics.


Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 148 ◽  
Author(s):  
Thekla I. Anastasiou ◽  
Manolis Mandalakis ◽  
Nikos Krigas ◽  
Thomas Vézignol ◽  
Diamanto Lazari ◽  
...  

The administration of antibiotics in aquaculture has raised concern about the impact of their overuse in marine ecosystems, seafood safety and consumers’ health. This “green consumerism” has forced researchers to find new alternatives against fish pathogens. The present study focused on 12 Mediterranean medicinal-aromatic plants as potential antimicrobials and antioxidant agents that could be used in fish aquaculture. In vitro assays showed that the essential oils (EOs) from all studied plants had anti-bacterial and antioxidant properties, with their efficacy being dependent on their chemical composition. More specifically, EOs rich in carvacrol, p-cymene and γ-terpinene exhibited not only the strongest inhibitory activity against the growth of bacterial pathogens (inhibitory concentration: 26–88 μg mL−1), but also the greatest total antioxidant capacity (ABTS: 2591–5879 μmole mL−1; CUPRAC: 931–2733 μmole mL−1). These compounds were mainly found in the EOs from Greek oregano (Origanum vulgare subsp. hirtum), Spanish oregano (Thymbra capitata) and savoury (Satureja thymbra) collected from cultivations in Greece. The specific EOs stand out as promising candidates for the treatment of bacterial diseases and oxidative stress in farmed fish. Further in vivo experiments are needed to fully understand the effects of EO dietary supplementation on fish farming processes.


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