Robust identification of low-Cd rice varieties by boosting the genotypic effect of grain Cd accumulation in combination with marker-assisted selection

2021 ◽  
pp. 127703
Author(s):  
Liang Sun ◽  
Ruigang Wang ◽  
Wenbang Tang ◽  
Yuchao Chen ◽  
Jieqiang Zhou ◽  
...  
Keyword(s):  
Marker Assisted Selection ◽  
Genotypic Effect ◽  
Rice Varieties ◽  
Robust Identification ◽  
Cd Accumulation

scholarly journals Stability and Adaptability Analysis of BRRI Developed Aus Varieties in Different Locations of Bangladesh

2019 ◽  
Vol 22 (1) ◽  
pp. 65-72
Author(s):  
NMF Rahman ◽  
MAA Mamun ◽  
R Ahmed ◽  
MI Hossain ◽  
MA Qayum ◽  
...  
Keyword(s):  
Grain Yield ◽  
Environmental Conditions ◽  
Environmental Effect ◽  
Genotypic Effect ◽  
Rice Varieties ◽  
Specific Adaptation ◽  
Genotype By Environment ◽  
Wide Adaptation ◽  
Main Effects ◽  
Better Than

The genotype by environment (G×E) interactions can be observed by differential genotypic responses to varied environmental conditions. Its effect is to limit the accuracy of yield estimates and complicate the identification of specific genotypes for specific environments. The frequently used model additive main effects and multiplicative interaction (AMMI) were used in this study to identify high yielding Aus rice varieties having wide adaptation and or specific adaptation to environment. Multi environment trials on eight Aus rice varieties were conducted at four environmental conditions in 2016. Genotype by environment (G×E) interactions contribution was much higher than the genotypic effect and environmental effect on grain yield. The genotypes BRRI dhan65, BRRI dhan48 and BRRI dhan43 display higher adaptability and stability. Therefore they are included in the study and recommended to be used in all environments. The variety BRRI dhan65 was identified as the most suitable variety with wider adaptability in the region Kushtia followed by BRRI dhan48 and BRRI dhan43. Latest varieties performed better than the oldest ones based on grain yield and could be replaced through ensuring the supply chain of new promising varieties of that locality. Bangladesh Rice j. 2018, 22(1): 65-72

scholarly journals Agronomic Management and Rice Varieties Controlling Cd Bioaccumulation in Rice

2019 ◽  
Vol 16 (13) ◽  
pp. 2376 ◽  
Author(s):  
Liangmei Chen ◽  
Wenge Wu ◽  
Fengxiang Han ◽  
Jiangxia Li ◽  
Wenling Ye ◽  
...  
Keyword(s):  
Grain Quality ◽  
Rice Varieties ◽  
Cd Accumulation ◽  
Safety Standards ◽  
Average Decrease ◽  
Rice Grains ◽  
Agronomic Management ◽  
Rice Yields ◽  
Selection Of

Selection of rice varieties and application of amendments are effective measures to ensure food safety. Here we report that in the non-Cd area, the grain quality of all rice varieties met the Chinese National Grain Safety Standards (CNGSS). In the high-Cd area, rice varieties showed significant different bioaccumulation of Cd with lower rice yields than those in non-Cd area with the average decrease of 31.1%. There was a negative correlation between grain Cd content and yields. A total of 19 rice varieties were selected as low Cd accumulating rice varieties and their Cd content met CNGSS in the low-Cd area. Six of them met CNGSS in the high-Cd area. The application of amendments significantly reduced Cd content in rice grains by 1.0–84.7% with an average of 52.6% and 13 of varieties met CNGSS. The amendments reduced available Cd content in soils by 1.1–75.8% but had no significant effects on rice yields. Therefore, the current study implied that proper agronomic management with selection of rice varieties and soil amendments was essential in controlling Cd accumulation in rice grains.

scholarly journals Combination of rice blast resistance genes in the genotypes of russian rice varieties with the use of marker assisted selection

2017 ◽  
Vol 15 (3) ◽  
pp. 54-63
Author(s):  
Pavel I. Kostylev ◽  
Elena V. Krasnova ◽  
Aleksandr A. Redkin ◽  
Elena V. Dubina ◽  
Zhanna M. Mukhina
Keyword(s):  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Blast Resistance ◽  
Pcr Analysis ◽  
Rice Varieties ◽  
Rice Blast Resistance ◽  
Second Stage ◽  
Grain Productivity ◽  
Third Stage ◽  
Blast Resistance Genes

Grain productivity of rice is significantly reduced by dangerous disease – blast. Therefore, the development of resistant high yielding rice varieties with the Pi group of genes is important. Use of molecular markers linked with the loci of resistance significantly optimizes the breeding process. The purpose of the research is to develop rice lines combining 2-6 loci of resistance to blast: Pi-1, Pi-2, Pi-33, Pi-ta, Pi-b, Pi-40 by molecular marking. Materials and Methods. As donors of resistance genes foreign samples were used, recipient – Russian varieties. In the studies we used micro-satellites markers and PCR analysis. Results. In the first stage of the research as a result of hybridization domestic lines with genes Pi-l, Pi-2, Pi-33 were obtained. At the second stage – hybrids with all 3 genes were developed. In the third stage genes Pi-b and Pi-ta and on the fourth – Pi-40 were introduced. Conclusion. As a result, rice genotypes, combining 6 loci of blast resistance were developed with use of marker assisted selection.

scholarly journals Comprehensive analysis of variation of cadmium accumulation in rice and detection of a new weak allele of OsHMA3

2019 ◽  
Vol 70 (21) ◽  
pp. 6389-6400 ◽  
Author(s):  
Cuiju Sun ◽  
Meng Yang ◽  
Yuan Li ◽  
Jingjing Tian ◽  
Yuanyuan Zhang ◽  
...  
Keyword(s):  
Cadmium Accumulation ◽  
Rice Cultivars ◽  
Cultivated Rice ◽  
Potential Threat ◽  
Rice Varieties ◽  
Cd Accumulation ◽  
Transfer Rates ◽  
Protein Levels ◽  
Analysis Of Variation ◽  
Cd Translocation

AbstractExcessive cadmium (Cd) accumulation in rice poses a potential threat to human health. Rice varieties vary in their Cd content, which depends mainly on root-to-shoot translocation of Cd. However, cultivars accumulating high Cd in the natural population have not been completely investigated. In this study, we analyzed the variation in Cd accumulation in a diverse panel of 529 rice cultivars. Only a small proportion (11 of 529) showed extremely high root-to-shoot Cd transfer rates, and in seven of these cultivars this was caused by two known OsHMA3 alleles. Using quantitative trait loci mapping, we identified a new OsHMA3 allele that was associated with high Cd accumulation in three of the remaining cultivars. Using heterologous expression in yeast and comparative analysis among different rice cultivars, we observed that this new allele was weak at both the transcriptional and protein levels compared with the functional OsHMA3 genotypes. The weak Cd transport activity was further demonstrated to be caused by a Gly to Arg substitution at position 512. Our study comprehensively analyzed the variation in root-to-shoot Cd translocation rates in cultivated rice and identified a new OsHMA3 allele that caused high Cd accumulation in a few rice cultivars.

scholarly journals The Road to Practical Application of Cadmium Phytoremediation Using Rice

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1926
Author(s):  
Ryuichi Takahashi ◽  
Masashi Ito ◽  
Tomohiko Kawamoto
Keyword(s):  
Heavy Metal ◽  
Genotypic Variation ◽  
Health Issues ◽  
Rice Varieties ◽  
Toxic Heavy Metal ◽  
Cd Accumulation ◽  
The Road ◽  
Metal Transporter ◽  
Marker Selection ◽  
Trait Locus

Cadmium (Cd) is a toxic heavy metal that causes severe health issues in humans. Cd accumulates in the human body when foods produced in Cd-contaminated fields are eaten. Therefore, soil remediation of contaminated fields is necessary to provide safe foods. Rice is one of the primary candidates for phytoremediation. There is a genotypic variation of Cd concentration in the shoots and grains of rice. Using the world rice core collection, ‘Jarjan’, ‘Anjana Dhan’, and ‘Cho-ko-koku’ were observed with a significantly higher level of Cd accumulation in the shoots and grains. Moreover, OsHMA3, a heavy metal transporter, was identified as a responsive gene of quantitative trait locus (QTL) for high Cd concentration in the shoots of these three varieties likewise. However, it is difficult to apply practical phytoremediation to these varieties because of their unfavorable agricultural traits, such as shatter and easily lodged. New rice varieties and lines were bred for Cd phytoremediation using OsHMA3 as a DNA marker selection. All of them accumulated Cd in the shoots equal to or higher than ‘Cho-ko-koku’ with improved cultivation traits. Therefore, they can be used for practical Cd phytoremediation.

scholarly journals Recent Advances in Rice Varietal Development for Durable Resistance to Biotic and Abiotic Stresses through Marker-Assisted Gene Pyramiding

2021 ◽  
Vol 13 (19) ◽  
pp. 10806
Author(s):  
Md Azadul Haque ◽  
Mohd Y. Rafii ◽  
Martini Mohammad Yusoff ◽  
Nusaibah Syd Ali ◽  
Oladosu Yusuff ◽  
...  
Keyword(s):  
Biotic Stress ◽  
Marker Assisted Selection ◽  
Target Genes ◽  
Durable Resistance ◽  
Gene Pyramiding ◽  
Molecular Techniques ◽  
Abiotic And Biotic Stress ◽  
Rice Varieties ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses

Abiotic and biotic stresses adversely affect rice growth, development and grain yield. Traditional rice breeding techniques are insufficient in modern agriculture to meet the growing population’s food needs on a long-term basis. The development of DNA markers closely linked to target genes or QTLs on rice chromosomes, and advanced molecular techniques, such as marker-assisted selection (MAS), have encouraged the evolution of contemporary techniques in rice genetics and breeding, such as gene pyramiding. Gene pyramiding refers to the act of combining two or more genes from multiple parents into a single genotype, which allows the overexpression of more than one gene for broad-spectrum abiotic and biotic stress resistance. Marker-assisted pedigree, backcrossing and pseudo-backcrossing methods can increase the conventional breeding speed by reducing the number of breeding generations in order to enhance the pyramiding process. Pyramiding is affected by several factors: the number of transferred genes; the range within gene and flanking markers; the number of chosen populations in every breeding generation; the features of genes and germplasms; and the potentiality of breeders to identify the target genes. Modern breeding methods, such as the marker-assisted backcrossing approach, have made gene pyramiding more precise and reliable for the development of stress-tolerant rice varieties in the coming decades. This review presents up-to-date knowledge on gene pyramiding schemes, marker-assisted gene pyramiding techniques, the efficiency of marker-assisted gene pyramiding and the advantages and limitations of gene pyramiding methods. This review also reports on the potential application of marker-assisted selection breeding to develop stress-tolerant rice varieties that stabilize abiotic and biotic stresses. This review will help rice breeders to improve yields by increasing rice productivity under abiotic and biotic stress conditions.

scholarly journals Diversity analysis of some selected rice genotypes through SSR- based molecular markers

2016 ◽  
Vol 12 (2) ◽  
pp. 307-311 ◽  
Author(s):  
MH Shahriar ◽  
AHK Robin ◽  
SN Begum ◽  
A Hoque
Keyword(s):  
Genetic Diversity ◽  
Marker Assisted Selection ◽  
Oryza Sativa L ◽  
Diversity Analysis ◽  
Growth Duration ◽  
Rice Varieties ◽  
Breeding Programs ◽  
Breeding Lines ◽  
Early Maturing ◽  
Rice Genotypes

An experiment was carried out to assess the genetic diversity of advanced rice (Oryza sativa L.) breeding lines using three SSR markers viz., RM147, RM167 and RM215. Thirty T- aman advanced breeding lines at F9 generation along with 4 check varieties were assessed. All three primers showed polymorphism. A total of 29 alleles were detected among the rice genotypes with an average of 9.67 alleles per locus. Polymorphism information content (PIC) ranged from 0.47 to 0.88 with an average of 0.71. A dendrogram was constructed based on total microsatellite polymorphism and 34 genotypes were grouped into four major clusters at 0.36 similarity co-efficient differentiating the early maturing genotypes from the late maturing types. The information about the genetic diversity might be utilized in future breeding programs for developing rice varieties with much shorter growth duration. The results also suggested that microsatellite markers which are linked to genes or QTLs responsible for growth duration properties are suitable tools for marker assisted selection (MAS) to select the rice genotypes of shorter growth duration.J. Bangladesh Agril. Univ. 12(2): 307-311, December 2014

scholarly journals Advances in the Uptake and Transport Mechanisms and QTLs Mapping of Cadmium in Rice

2019 ◽  
Vol 20 (14) ◽  
pp. 3417 ◽  
Author(s):  
Jingguang Chen ◽  
Wenli Zou ◽  
Lijun Meng ◽  
Xiaorong Fan ◽  
Guohua Xu ◽  
...  
Keyword(s):  
Transport Mechanisms ◽  
Rice Varieties ◽  
Cd Uptake ◽  
Genetic Traits ◽  
Cd Accumulation ◽  
Existing Problems ◽  
The Status ◽  
Harmful Effects ◽  
Mapping Populations ◽  
Qtls Mapping

Cadmium (Cd), as a heavy metal, presents substantial biological toxicity and has harmful effects on human health. To lower the ingress levels of human Cd, it is necessary for Cd content in food crops to be reduced, which is of considerable significance for ensuring food safety. This review will summarize the genetic traits of Cd accumulation in rice and examine the mechanism of Cd uptake and translocation in rice. The status of genes related to Cd stress and Cd accumulation in rice in recent years will be summarized, and the genes related to Cd accumulation in rice will be classified according to their functions. In addition, an overview of quantitative trait loci (QTLs) mapping populations in rice will be introduced, aiming to provide a theoretical reference for the breeding of rice varieties with low Cd accumulation. Finally, existing problems and prospects will be put forward.

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