The influence of an inoculant/enzyme preparation as an additive for grass silage offered in combination with three levels of concentrate supplementation on performance of lactating dairy cows

1993 ◽  
Vol 56 (3) ◽  
pp. 301-310 ◽  
Author(s):  
E. J. Smith ◽  
A. R. Henderson ◽  
J. D. Oldham ◽  
D. A. Whitaker ◽  
K. Aitchison ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Formic Acid ◽  
Milk Production ◽  
Enzyme Preparation ◽  
Latin Square ◽  
Metabolizable Energy ◽  
Water Soluble ◽  
Energy Output ◽  
Soluble Carbohydrates ◽  
Concentrate Supplementation

AbstractThree silages were prepared from the primary growth of a predominantly perennial ryegrass sward (dry matter (DM) 175 g/kg; crude protein 142 g/kg DM; water-soluble carbohydrates (WSC) 243 g/kg DM). Herbage was wilted for 24 h and then treated with either an inoculant/enzyme preparation (Lactobacillus plantarum, Streptococcus faecium and Pediococcus acidilactici), formic acid applied at 4·2 lit (Add F, BP Chemicals) or no additive. Time series analysis of laboratory silages revealed that different patterns of fermentation had been achieved: formic acid treatment resulted in high levels of residual WSC and low levels of lactic acid, indicative of an inhibited fermentation, whilst treatment with the inoculant/enzyme preparation resulted in high levels of lactic acid with a low level of residual sugars, as expected with an enhanced fermentation. Analysis of the material ‘as fed’ showed that losses in the WSC content of the formic acid-treated silage had occurred in the clamp. Secondary fermentation of lactic to acetic acid was apparent in the untreated silage, but not in the inoculant/enzyme-treated silage. Digestibility, as determined using Greyface wether lambs, was marginally higher for both additive treatments when compared with the untreated silage. An evaluation of the silages for milk production was carried out at three levels of concentrate supplementation using 18 Ayrshire × British Friesian cows in a replicated 3×3 Latin-square design experiment. Treatment with formic acid resulted in significantly higher DM intakes, but this was not reflected in milk energy output. Cows offered the inoculant/enzyme-treated silage partitioned energy away from milk production toward body tissue deposition (average milk yields 19·9, 19·9 and 15·2 kg/day, and weight gain 0·26, 0·38 and 0·81 kg/day for the untreated, formic acid and inoculant/enzyme-treated silages respectively). The reason for this is not clear, but it is postulated that microbial capture of degraded nitrogen may have been impaired with the inoculant/enzyme-treated silage, resulting in an imbalance in metabolizable protein: metabolizable energy.

Beef production from formic acid-treated and wilted silages

1976 ◽  
Vol 22 (2) ◽  
pp. 217-223 ◽  
Author(s):  
C. E. Hinks ◽  
I. E. Edwards ◽  
A. R. Henderson
Keyword(s):  
Weight Gain ◽  
Formic Acid ◽  
Dry Matter ◽  
Nitrogen Retention ◽  
Live Weight ◽  
Metabolizable Energy ◽  
Water Soluble ◽  
Dry Matter Intake ◽  
Soluble Carbohydrates ◽  
Live Weight Gain

SUMMARY1. Herbage was ensiled, fresh at 17% DM or wilted to 32% DM, with and without the addition of formic acid (19·2 kg/tonne DM). The silages were fed individually ad libitum to 36 fifteen-month-old British Friesian steers of about 280 kg initial live weight (nine animals per treatment).2. Wilting and the application of formic acid both inhibited fermentation, resulting in silages with higher levels of water-soluble carbohydrates, lower concentrations of volatile nitrogen and lower organic acid contents.3. There were no significant dry matter × formic acid interactions in dry-matter intake or daily live-weight gain.4. Treatment with formic acid did not enhance significantly either dry-matter intake or daily live-weight gain. Furthermore, it had little effect on digestibility, nitrogen retention or metabolizable energy (ME) concentration.5. Wilting before ensiling increased dry-matter intake from 5·0 to 8·3 kg/head per day with consequent marked increases in ME intake, nitrogen retention and live-weight gain. Wilting, however, had little effect on digestibility, ME concentration, or the efficiency with which dietary nitrogen intake was retained.

Ensiling grass and strain mixtures: effect of an enzyme additive and urea on chemical composition and nutritive value for sheep

1989 ◽  
Vol 1989 ◽  
pp. 102-102
Author(s):  
R.C. Jakhmola ◽  
J.R. Weddell ◽  
J.F.D. Greenhalgh
Keyword(s):  
Chemical Composition ◽  
Enzyme Preparation ◽  
Nutritive Value ◽  
Latin Square ◽  
Water Soluble ◽  
Barley Straw ◽  
Soluble Carbohydrates ◽  
Feeding Period ◽  
Cellulase Enzyme ◽  
Positive Effects

Straw mixed with grass before ensiling absorbs excess water and reduces effluent, but dilutes nutrients like crude protein and water soluble carbohydrates, and increases cell wall constituents of the mixture. Earlier studies by the authers showed that treating straw with urea before ensiling with grass increased the digestibility of grass+straw silages. A cellulase enzyme preparation had small but positive effects on fermentation and digestibility. The present paper describes the effect of treating straw with urea and a cellulase enzyme preparation on chemical composition and nutritive value of Silages.Shredded barley straw was treated with urea (60 kg/t of straw dry matter) and a cellulase enzyme preparation (FS01, Forum Feeds Finland; 2 1/t of straw), just before mixing with the precision chopped perennial ryegrass in the ratio of 40:60 on a DM basis, in a 2 X 2 factorial experiment. All the four premixes were ensiled in polythene silos ( 1 t capacity). Eight male sheep were fed four silages in a 4 X 4 latin square design. Each feeding period consisted of a 4-day adjustment period, a 10-day preliminary feeding period and a 7-day period for measurement of digestibility.

scholarly journals Formic acid treated whole crop barley and wheat silages in dairy cow diets: effects of crop maturity, proportion in the diet, and level and type of concentrate supplementation

2008 ◽  
Vol 18 (3-4) ◽  
pp. 234 ◽  
Author(s):  
S. JAAKKOLA ◽  
E. SAARISALO ◽  
T. HEIKKILÄ
Keyword(s):  
Formic Acid ◽  
Milk Production ◽  
Milk Yield ◽  
Weather Conditions ◽  
Protein Supplementation ◽  
Concentrate Supplementation ◽  
Fermentation Quality ◽  
Yield Responses ◽  
Crop Maturity

Three trials in dairy cows were carried out to study the effects of replacing grass silage (GS) with wholecrop silage (WCS) made of barley (BS) or wheat (WS) harvested at dough stage with a dry matter (DM) concentration of 300-450 g kg-1. All silages were ensiled using a formic acid based additive 5 l t-1. Milk production responses to energy and protein supplementation of diets were studied. In Exp. 1, BS replaced GS at the rates of 0, 200, 400 or 600 g kg-1 forage DM. Also 10 kg of concentrate containing 0 or 2 kg of rape seed meal was fed. In Exp. 2, barley was harvested at three times (BS1, BS2, BS3) at one week intervals. Barley silages were fed as a mixture with GS (40:60) and in addition BS2 and GS alone. Silages were supplemented with a cereal based farm-made concentrate (FC) or a commercial compound having a lower concentration of starch than FC. In Exp. 3, barley and wheat were harvested at two week intervals, fed as a mixture with GS (40:60) and supplemented with low or high amount of concentrate. The fermentation quality of whole crop silages was good. Weather conditions and maturity affected the proportion of ear in the crop and subsequently the ratio of non-structural carbohydrates to NDF in the silage. The inclusion of WCS depressed diet OM digestibility depending on the digestibility of GS and the proportion of WCS in the diet. However, mixing GS and WCS did not depress intake. Subsequently the use of mixtures maintained or even increased milk yield as compared with GS diet in Exp. 2 and 3. In Exp. 1, higher proportions (400, 600 g kg-1) of BS decreased milk yield. Minor effects of growth stage on milk production were observed with barley whereas delaying wheat harvest increased milk yield. Different types and levels of concentrate induced mainly similar intake and milk yield responses with diets based on GS alone or on mixtures of GS and WCS. The synergistic effect of mixing GS and WCS was more positive in experiments where the protein concentration of concentrate was high (200 g kg-1 DM).;

scholarly journals Effects of biological and chemical additives on fermentation progress in maize silage

2017 ◽  
Vol 62 (No. 7) ◽  
pp. 306-312 ◽  
Author(s):  
Y. Tyrolová ◽  
L. Bartoň ◽  
R. Loučka
Keyword(s):  
Lactic Acid ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Maize Silage ◽  
Chemical Additives ◽  
Chemical Additive ◽  
Treatment Groups ◽  
Acid Ratio ◽  
Group B ◽  
Additive Control

The objective of this study was to evaluate the effects of bacterial and chemical additives on the number of lactic acid bacteria (LAB) and on fermentation indicators in whole maize silage at 1, 3, 5, 10, and 90 days of fermentation. Maize forage was harvested at approximately 34% dry matter (DM) and treated with (1) no additive (control; C); (2) bacterial inoculant (2 g/t of forage; B) containing the homofermentative LAB Lactobacillus plantarum, Lactobacillus paracasei, and Pediococcus pentosaceus (1.5 × 10<sup>11</sup> cfu/g of inoculant); and (3) chemical additive (4 l/t of forage; CH) containing formic acid, propionic acid, ammonium formate, and benzoic acid. Both treatments decreased pH of silage at day 1 of ensiling (P &lt; 0.05), and the lowest value of 4.34 was observed in the CH-treated silage. All silages were well fermented and had pH &lt; 4.0 by day 10 of fermentation. The concentration of lactic acid and the lactic acid : acetic acid ratio increased over time in all treatment groups, and the highest values were 87.5 and 3.62 g/kg of DM, respectively, observed for group B at day 90 (P &lt; 0.05). The concentrations of water-soluble carbohydrates were higher (P &lt; 0.05) for CH compared to C and B at days 3, 5, 10, and 90 of fermentation. The CH silage had fewer LAB (P &lt; 0.05) than did either C or B silages regardless of the days of fermentation. Both additives used in the present study improved fermentation dynamics of the whole crop maize silage.

The associative effect of level of energy and protein intake in the dairy cow

1970 ◽  
Vol 37 (3) ◽  
pp. 481-491 ◽  
Author(s):  
F. J. Gordon ◽  
T. J. Forbes
Keyword(s):  
Energy Intake ◽  
Milk Yield ◽  
Protein Level ◽  
Protein Intake ◽  
Latin Square ◽  
Metabolizable Energy ◽  
Energy Output ◽  
Additional Energy ◽  
Protein Nitrogen ◽  
Low Protein

SummaryEight lactating cows were used in a Latin square experiment, to study the associative effects of level of energy and protein intake on milk yield and composition. Four diets were used, supplying 80 and 120% of estimated energy requirements and 80 and 120% of estimated protein requirements. The level of energy intake significantly affected milk yield, milk energy output, percentage butterfat, ash and non-protein nitrogen. The level of protein intake only significantly affected milk energy output and the non-protein nitrogen content of the milk. Although only the interaction of the effects of energy and protein intake on the milk content of solidsnot-fat (SNF) and ash was significant, it was evident that the effect of each of these factors on milk yield or composition was related to the level of the other in the diet.Input-output relationships within each protein level were used to compute the response in milk energy output and bodyweight change to a change in energy intake. These showed a greater partitioning of additional energy toward milk energy output with the high than with the low protein level. Multiple regression analysis within each level of protein intake was used to partition energy intake between that used for maintenance, milk energy output and liveweight change. The results showed efficiencies of utilization of metabolizable energy for milk output of 63 and 50% on the high- and low-protein diets, respectively.Nitrogen balance data are presented.

The effect of genetic merit and concentrate proportion in the diet on nutrient utilization by lactating dairy cows

1999 ◽  
Vol 132 (4) ◽  
pp. 483-490 ◽  
Author(s):  
C. P. FERRIS ◽  
F. J. GORDON ◽  
D. C. PATTERSON ◽  
M. G. PORTER ◽  
T. YAN
Keyword(s):  
Dairy Cows ◽  
Energy Intake ◽  
Milk Production ◽  
Milk Fat ◽  
Nutrient Utilization ◽  
Metabolizable Energy ◽  
Energy Utilization ◽  
Energy Output ◽  
Genetic Merit ◽  
Gross Energy

Sixty Holstein/Friesian dairy cows, 28 of high genetic merit and 32 of medium genetic merit, were used in a continuous design, 2 (cow genotypes)×4 (concentrate proportion in diet) factorial experiment. High and medium merit animals had Predicted Transmitting Abilities for milk fat plus protein yield, calculated using 1995 as the base year (PTA95 fat plus protein), of 43·3 kg and 1·0 kg respectively. Concentrate proportions in the diet were 0·37, 0·48, 0·59 and 0·70 of total dry matter (DM), with the remainder of the diet being grass silage. During this milk production trial, 24 of these animals, 12 from each genetic merit, representing three animals from each concentrate treatment, were subject to ration digestibility, and nitrogen and energy utilization studies. In addition, the efficiency of energy utilization during the milk production trial was calculated.There were no genotype×concentrate level interactions for any of the variables measured (P>0·05). Neither genetic merit nor concentrate proportion in the diet influenced the digestibility of either the DM or energy components of the ration (P>0·05). When expressed as a proportion of nitrogen intake, medium merit cows exhibited a higher urinary nitrogen output and a lower milk nitrogen output than the high merit cows. Methane energy output, when expressed as a proportion of gross energy intake, was higher for the medium than high merit cows (P<0·05), while urinary energy output tended to decrease with increasing proportion of concentrate in the diet (P<0·05). In the calorimetric studies, neither heat energy production, milk energy output and energy retained, when expressed as a proportion of metabolizable energy intake, nor the efficiency of lactation (kl), were affected by either cow genotype or concentrate proportion in the diet (P>0·05). However when kl was calculated using the production data from the milk production trial the high merit cows were found to have significantly higher kl values than the medium merit cows (0·64 v. 0·59, P<0·05) while k l tended to fall with increasing proportion of concentrate in the ration (P<0·05). However in view of the many assumptions which were used in these latter calculations, a cautious interpretation is required.

Insulin response to feeding forage with varying crude protein and amino acid content in horses at rest and after exercise

2013 ◽  
Vol 9 (3-4) ◽  
pp. 209-217 ◽  
Author(s):  
S. Ringmark ◽  
A. Jansson
Keyword(s):  
Body Weight ◽  
Crude Protein ◽  
Plasma Insulin ◽  
Insulin Response ◽  
Latin Square ◽  
Amino Acid Content ◽  
Nutrient Content ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Plasma Insulin Response

This study assessed the insulin response to forage intake with varying crude protein (CP) content in horses at rest and after exercise. Six geldings were fed three grass haylage-only diets for 7 days according to a 3×3 Latin square design. On day 7, blood samples were collected before and for 120 min after feeding 15% of the daily allowance before exercise (feeding A) and after standardised exercise (feeding B). Feed samples were collected before each feeding. Dry matter (DM) and nutrient content varied (DM: 37-58%, water-soluble carbohydrates minus fructans (WSC-f): 3-12% of DM, CP: 10-15% of DM) which resulted in a variation in nutrient intake within haylage batches. Based on individual CP and WSC-f intakes, intake groups were therefore formed (low and high CP intake; ≤ and ≯180 g CP/100 kg body weight, respectively and low and high WSC-f intake; ≤ and ≯100 g/100 kg body weight, respectively). Amino acids were analysed and intakes were generally higher in the high CP group than in the low CP group. An ANOVA model including horse, CP group and WSC-f intake explained 95% of the variation in plasma insulin response compared to 87% using a model including horse and WSC-f group alone. The plasma insulin area under curve (AUC) following feeding A tended to be higher in the high CP group than in the low CP group (P=0.08), but there was no difference after feeding B. Plasma glucose AUC was not affected by CP group (P≯0.05). The study indicates that the post-prandial plasma insulin response in horses fed a forage-only diet is increased by high WSC-f intake but may also be increased by high CP intake, at least at rest. However, due to the low number of observations further studies are needed.

An evaluation of an inoculant of Lactobacillus plantarum as an additive for grass silage for dairy cattle

1990 ◽  
Vol 51 (1) ◽  
pp. 1-13 ◽  
Author(s):  
C. S. Mayne
Keyword(s):  
Formic Acid ◽  
Lactobacillus Plantarum ◽  
Energy Intake ◽  
Milk Yield ◽  
Acid Treatment ◽  
Water Soluble ◽  
Energy Output ◽  
Yield Response ◽  
Absorbent Polymer ◽  
Polymer Treatments

ABSTRACTHerbage from the first regrowth of perennial ryegrass based swards was direct-ensiled following treatment with either an inoculant of Lactobacillus plantarum (Ecosyl, Imperial Chemical Industries pic) at 3·0 1/t, formic acid (850 g/kg) at 2·9 1/t, or no additive (control). During harvesting, alternate loads of inoculant material were treated with an absorbent polymer (ammonium polyacrylamide) at the rate of 1 kg/t herbage and ensiled in separate 100-t capacity silos. The mean dry matter (DM) and water soluble carbohydrate concentrations of herbage used for the four treatments was 157 and 120 g/kg respectively. Lactic acid levels post ensiling increased more rapidly in inoculant-treated herbage than with the other treatments. Formic acid and inoculant-treated silages were well preserved whereas control and inoculant-plus-polymer silages were only moderately well preserved. Losses of DM during ensilage were greater with the formic acid treatment with DM recovery values of 0·78, 0·72, 0·76 and 0·73 for the control, formic acid, inoculant and inoculant-plus-polymer silages respectively. Treatment of herbage with an absorbent polymer prior to ensiling resulted in a proportional reduction in effluent volume of 0·2 whereas formic acid treatment increased effluent flow by 0·28. The silages were evaluated in a changeover design experiment with two periods each of 4 weeks duration, using 24 British Friesian dairy cows. Animals were housed in individual stalls and in addition to the treatment silages, received 5 kg/day of supplement containing 193 g crude protein per kg DM. Silage intakes were increased by proportionately 0·10, 0·14 and 0·05 respectively with the formic acid, inoculant and inoculant-plus-polymer treatments compared with the control. The increased silage intakes with the inoculant treatment were reflected in an increased milk yield of 1·1 kg milk per day whereas formic acid and inoculant-plus-polymer treatments had no significant effect, although formic acid treatment did result in a significant increase in milk fat concentration. There were no major differences between treatments in energy or nitrogen digestibility, when determined on a complete ration basis. In conclusion, a large milk yield response was obtained as a result of treatment of herbage with inoculant prior to ensiling and this resulted from increased silage and hence energy intake. Treatment with formic acid increased silage and energy intake but had no effect on milk energy output.

Intake, digestibility and aerobic stability of barley silage inoculated with mixtures of Lactobacillus plantarum and Enterococcus faecium

1995 ◽  
Vol 75 (3) ◽  
pp. 425-432 ◽  
Author(s):  
T. A. McAllister ◽  
L. B. Selinger ◽  
L. R. McMahon ◽  
H. D. Bae ◽  
T. J. Lysyk ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Enterococcus Faecium ◽  
Volatile Fatty Acids ◽  
Average Daily Gain ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
Soluble Carbohydrates ◽  
Aerobic Stability ◽  
Barley Silage

The effect of ensiling barley treated with two bacterial inoculants containing mixtures of Lactobacillus plantarum and Enterococcus faecium (1.0 × 105 cfu g−1 as fed silage) on the nutritional value and aerobic stability of barley silage was examined. Inoculants differed in the strains they contained and were originally selected by Pioneer Hi-Bred International for use with corn or alfalfa silage, SILA-BAC® (1174), or with grass silage (X2637). Concentrations of water-soluble carbohydrates were higher (P < 0.05) in inoculated than in control silages. Although inoculants appeared to increase the numbers of lactic acid producing bacteria (LAB) at ensiling, post-ensiling numbers (cfu g−1) of yeasts and molds were lower (P < 0.05) in inoculated than in control silages. Lactic acid concentrations and pH were similar among the silages and variations m the growth of yeast and mold populations could not be explained by differences in the production of volatile fatty acids (VFA) among silages. Inoculation of barley silage with either inoculant increased (P < 0.01) the average daily gain of lambs. A digestibility experiment with 12 growing ram lambs showed that inoculants did not alter (P > 0.05) DM intake, feed efficiency or the digestion of DM, organic matter, acid detergent fiber (ADF) and neutral detergent fiber (NDF). Nitrogen intake and retention were greater (P < 0.05) in lambs fed silage inoculated with 1174 as compared with control silage. Yeast populations were increased (P < 0.05) in control and 1174 after 2 d of exposure to air but it required 13 d for a similar yeast population to be established in X2637 silage. Increases in the mold populations within the silages were noted after 2, 5 and 13 d of exposure to air for control, 1174 and X2637, respectively. The temperature of control silage increased (P < 0.05) 2 d after exposure to air, whereas increases in temperature were delayed for 4 d in 1174 and 8 d in X2637. Temperatures rose as high as 30 °C in control silage, but did not exceed 24 °C in inoculated silages during the 13 d period. Key words: Barley silage, inoculant, digestion, aerobic stability, sheep, gain

scholarly journals The effect of lactic acid additive on the quality and chemical composition of meadow grass silage

2003 ◽  
Vol 19 (3-4) ◽  
pp. 61-69
Author(s):  
Jan Pyś ◽  
Wladislaw Migdal ◽  
Branislav Zivkovic ◽  
Olga Kosovac ◽  
Mihal Fabjan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Crude Protein ◽  
Dry Matter ◽  
Water Solution ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Total N ◽  
Grass Silage ◽  
Fermentation Quality ◽  
Dominant Grasses

In the experiment, forage from the 1st cut of meadow grasses mown at the heading stage of dominant grasses was ensiled. The control silages were made from fresh forage (dry matter 223.5 g?kg-1) and wilted forage (dry matter 351.9 g?kg-1). The experimental silages were made from fresh forages supplemented with a 5% water solution of lactic acid (0.5 and 0.7 l?100 kg-1 of forage) and 8% water solution of lactic acid (0.4 and 0.6 l?100 kg-1 of forage). The highest contents of crude protein, water-soluble carbohydrates and energy were found in silages supplemented with 5% lactic acid applied at 0.7 l?100 kg-1 of forage and in silages supplemented with 8% lactic acid applied at 0.6 l?100 kg-1 of forage. The above silage variants were also characterized by the lowest content of NH3-N in total-N, butyric acid and acetic acid, as well as the highest contents of lactic acid and the best indicators of fermentation quality. No important differences were found between silages made from wilted forage and silages made with lactic acid supplement.

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