The dietary arachidonic acid improved growth and immunity of honey bee (Apis mellifera ligustica)

Author(s):  
Jing Yu ◽  
Weixing Zhang ◽  
Xuepeng Chi ◽  
Wenfeng Chen ◽  
Zhenfang Li ◽  
...  

Abstract Honeybees cannot synthesize arachidonic acid (ARA) themselves, only obtain it from food. Most pollen is deficient or contains a small amount of ARA. The necessity of supplementary ARA in bees’ diet has not been studied. The objective of this study was to investigate the effects of dietary ARA levels on the growth and immunity of Apis mellifera ligustica. A total of 25 honeybee colonies were randomly assigned to five dietary groups which were fed basic diets supplemented with 0, 2, 4, 6, and 8% of ARA. The diet with 4% ARA improved the body weight of newly emerged worker bees compared with the control group. Supplement of ARA in honeybee diets changed the fatty acid composition of honeybee body. SFA and MUFA contents of bees’ body declined, and PUFA content rised in the ARA group. Compared with the control group, the supplement of ARA in honeybee diets increased the contents of ARA, C22:6n-3 (DHA) and C18:3n-6 in bees’ body significantly, but decreased the contents of C16:1 and C18:3n-3. The diet supplied with 4% ARA reduced the mortality rate of honeybee infected with Escherichia coli. The activity of immune enzymes (phenoloxidase, antitrypsin, and lysozyme) and the mRNA expression levels of immune genes (defensin-2, toll, myd88, and dorsal) were improved by ARA diets to varying degrees depending on the ARA levels, especially 4% ARA. These results suggested that dietary ARA could improve the growth, survival, and immune functions of honeybees. Supplement of ARA in bees’ diet would be valuable for the fitness of honeybees.

2021 ◽  
Vol 12 ◽  
Author(s):  
Azmat Rozjan ◽  
Weibi Shan ◽  
Qiaoling Yao

ObjectiveThis study aims to investigate whether hypoxia-inducible factor 1α (HIF1α) in the neurons of the mediobasal hypothalamus is involved in the regulation of body weight, glucose, and lipid metabolism in mice and to explore the underlying molecular mechanisms.MethodsHIF1αflox/flox mice were used. The adeno-associated virus that contained either cre, GFP and syn, or GFP and syn (controls) was injected into the mediobasal hypothalamus to selectively knock out HIF1α in the neurons of the mediobasal hypothalamus. The body weight and food intake were weighed daily. The levels of blood glucose, insulin, total cholesterol (TC), triglyceride (TG), free fatty acid (FFA), high-density lipoprotein (HDL), and low-density lipoprotein (LDL)were tested. Intraperitoneal glucose tolerance test (IPGTT) was performed. The insulin-stimulated Akt phosphorylation in the liver, epididymal fat, and skeletal muscle were examined. Also, the mRNA expression levels of HIF1α, proopiomelanocortin (POMC), neuropeptide Y (NPY), and glucose transporter protein 4 (Glut4) in the hypothalamus were checked.ResultsAfter selectively knocking out HIF1α in the neurons of the mediobasal hypothalamus (HIF1αKOMBH), the body weights and food intake of mice increased significantly compared with the control mice (p < 0.001 at 4 weeks). Compared with that of the control group, the insulin level of HIF1αKOMBH mice was 3.5 times higher (p < 0.01). The results of the IPGTT showed that the blood glucose level of the HIF1αKOMBH group at 20–120 min was significantly higher than that of the control group (p < 0.05). The serum TC, FFA, HDL, and LDL content of the HIF1αKOMBH group was significantly higher than those of the control group (p < 0.05). Western blot results showed that compared with those in the control group, insulin-induced AKT phosphorylation levels in liver, epididymal fat, and skeletal muscle in the HIF1αKOMBH group were not as significantly elevated as in the control group. Reverse transcription-polymerase chain reaction (RT-PCR) results in the whole hypothalamus showed a significant decrease in Glut4 mRNA expression. And the mRNA expression levels of HIF1α, POMC, and NPY of the HIF1αKOMBH group decreased significantly in ventral hypothalamus.ConclusionsThe hypothalamic neuronal HIF1α plays an important role in the regulation of body weight balance in mice under normoxic condition. In the absence of hypothalamic neuronal HIF1α, the mice gained weight with increased appetite, accompanied with abnormal glucose and lipid metabolism. POMC and Glut4 may be responsible for this effect of HIF1α.


Author(s):  
L. Dyachenko ◽  
E. Syvachenko ◽  
T. Syvyk

In two scientific-field experiments with broiler chicken cross-breeding ”Kobb-500", the goal was set to learn the influence of different dosages, forms and types of feeding the acidifier FRA LBB DRY onto the contents of the microflora of intestine, retention and productivity of broiler chickens. The first experiment was done using three groups-analogs of broiler chickens, 46 birds per group. The broiler chickens of the first group were the control group. This group was fed a regular mixed feed. The second and third groups were fed the same mixed feed with an addition of the acidifier of FRA LBB DRY feed added; 3kg per ton of feed for the 2-nd, and 5 kg per ton for the third group. The second experiment was done using 6 similar groups-analogs of broiler chickens, 100 birds per group, with an equal ratio of male to female chicken. Throw-out the experiment all six groups were fed the same amount of fully nutritional mixed feed, in accordance with their age growth groups. As far as drinking went, the groups were hydrated differently. Birds of the first group were getting plain water, broiler chickens of the 2, 3, 4, and 5 groups were getting a mix of FRA LBB DRY acidifier in the quantities of 1,0; 1,33; 1,66 and 2,0 ml/L accordingly. The last group, group 6, was getting a dose of liquid Norfolk antibiotic in the quantity of 1 ml/L. As the bacteriological experiments have shown, after 7 days of feeding different doses of dry acidifier, the stool sample of the second and third groups of broiler chickens, when compared to the first group, had shown to contain less undesirable microflora in particular Escherichia coli – by 26,7 and 29,0 %, enterococcus by 21,4 and 24,7 % with a high probability of difference (P<0,01). At the same time the test samples contained higher concentration of useful microflora such as Bifidobacterium and lacto bacteria. The difference, when compared to the- test sample, was 18,3–24,1 % (P<0,01) and 16,5-17,8 % (P<0,05). In the second experiment adding acidifier FRA LBB DRY to water for the 2, 3, 4, and 5-th groups by 1,0; 1,33; 1,66 and 2 ml/l of water had caused a decrease of escherichia coli by 12,7 % (P<0,05), 17,4 % (P<0,01), 18,8 % (P<0,01) and 16,0 % (P<0,05), enterococcus by 14,9; 20,9; 17,8; and 16,9 % with a high degree of probability (P<0,01), at the same time increasing the amount of bifidobacteria by 19,1; 20,6; 17,9 and 21,3 %, (P<0,01) and lactobacteria by 14,4; 16,5; 15,8; 12,4 % (P<0,01). The changes that had taken place in the contents of the microflora of intestine obviously had a positive effect on digestion and processing of nutrients, which, as a result, had improved the upkeep and productivity of the broiler chickens which were exposed to the acidifier. In the first experiment departure of the broiler chickens was almost three times less than that of a control group. In the second experiment the upkeep of broilers in test groups was larger than the control by 6–7 absolute percent. Adding an acidifier into the mixed feed also increased the rate of growth in birds. Case in point, in the first experiment the growth of the body mass of one broiler of the 2-nd and 3-rd test groups was by 5,05 and 5,23 % larger than the control group. In the second experiment, both absolute and daily weight gains of 2–5-th groups were by 3,8-4,5 % larger than those of the control group. As far as comparing the expenses in terms of quantities of food per 1 kg of gains, both experiments have shown that test groups 2–3 and 2–5 consumed 3,4 and 2,7–4,3 % less than the control group. Among the objective measures that show the efficiency of the experiment, we can use the European efficiency index, which in first and second experiments was 37,8-38-6, and 32,0-50,1 points higher than the control group. Alongside with the acidifier, the experiment was also set to text the effectiveness of the use of the Norfolk antibiotic, which was given to the birds in group 6 of the second experiment. The data had shown that by absolute and daily weight gain, feed conversion, livestock retention, and European efficiency index, the birds in the 6th group definitely surpassed the control group in all categories, but fell short of the other experiment groups in almost all categories. This data lets us conclude that there is a good possibility that we can exchange the antibiotics for acidifier, with little to no loss in productivity. In summary, the data of this microbiological research shows that including acidifier into a fixed feed has a positive impact on the broiler chicken intestines microflora, which could quite possibly be one of the factors that improves the digestion and consumption process and an improved upkeep of the birds as a result. By the general evaluation of the results of the experiment, the optimal dosage of dry and liquid acidifier FRA LBB DRY for the broiler chickens can be considered 3kg/tons of mixed feed or 1,33 –1,66 ml/l of water. As a perspective of further development, the research might look into a search of acidifiers of a different biochemical nature and of a more potent action. Key words: broiler chickens, acidifier, antibiotic, microflora of the intestines, productivity.


2019 ◽  
Vol 21 (95) ◽  
pp. 55-61 ◽  
Author(s):  
I. Salamon ◽  
J. Poracova ◽  
M. Hrytsyna

The article describes the results of the study of the physiological effect on the organism of pigs - mammals and weaned piglets. Feed additives were made from the essential oil of Origanum vulgare “Aditívum do kŕmnych zmesí na báze pamajoránu”. It has been established that O. vulgare dry grass contains essential oils (0.15–0.50%), where carvacrol, thymol, cymol and other terpenes are main components. Extract of O. vulgare essential oil has a pronounced inhibitory effect on the enteropathogenic bacteria Escherichia coli hemolytic and Salmonella enteritica var. Enteritidis. In quantifying, it was found that 20% of the essential oil extract has inhibition role, which is 4 times more effective than 100%, has the largest inhibitory role. The resistant symbiotic bacterium of the gut Enterococcus faecium M-7 was not affected by essential oil. The following indicators were used to establish the physiological effect of herbal supplements on the body of piglets: increasment in body weight, resistance of piglets to diseases, the state of intestinal microflora. A control and two experimental groups were added for the experiment, where the phyto-additive was included in to the feed: the first consisted of bacteria Lactobacillus plantarum with 0.05% extract of O. vulgare (LO) essential oil and the second – only 0.05% essential oil extract О. vulgare. (O). In the two experimental groups, the pigs' health was good and their mortality was not observed either before or after weaning. The incidence was 18% before weaning and 36% after weaning in the control group. The average daily weight gaining was significant in both groups (31–36%). Moreover, piglets were larger at birth and before weaning they increased in weight to 8 times, whereas over the next 24 days their weight increased only to 50%. The difference in gaining weight between the two expirimantal groups was insignificant. The use of the herbal supplement LO is more effective than O, because the stability index of the gut microbiota gradually increases from 2.2 to 3.37 due to decrease in the number of opportunistic bacteria Escherichia coli and enterobacteria and growth of lactobacilli. Therefore, a phyto-supplement consisting of Lactobacillus plantarum bacteria with 0.05% O. vulgare (LO) essential oil extract can be recommended as a probiotic to feed mammals and weaned pigs for improving health and disease prevention.


2016 ◽  
Vol 60 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Kai Wang ◽  
Yu Qi ◽  
Shushuai Yi ◽  
Zhihua Pei ◽  
Na Pan ◽  
...  

Abstract Introduction: The aim of the experiment was to establish the enterotoxigenic Escherichia coli K88 (ETEC K88)-induced BALB/c mouse duodenum inflammation model. Material and Methods: Mice were administered different concentrations of E. coli K88 (1.0 × 107-109 CFU/mL) for 3 d by means of an esophageal catheter. Results: The results showed that the treated group expressed several significant clinical symptoms, such as reduced dietary demands and weight loss, an increased presence of IL-1α, TNF-α, and MPO in the peripheral blood, and some pathological changes in the duodenum. On the 6th-8th days, the body weight of the mice was the lowest. On the 8th day, there were significant differences in IL-1α, TNF-α, and MPO levels compared to the control group (P < 0.05), the gap between the duodenum mucous layer and the muscular layer had widened, the number of goblet cells was increased, and the inflammatory infiltrate and inflammation changes in the lamina propria and the mucous layer were the most obvious. Conclusion: The duodenum inflammation was the most severe on day 8; thus, the model was successfully established. In addition, varying concentrations of ETEC K88 did not significantly influence the duodenum inflammation (P > 0.05).


2020 ◽  
Vol 72 (5) ◽  
pp. 1586-1598
Author(s):  
M.A.F. Sá ◽  
T.F.G. Lima ◽  
R.C.L. Morais ◽  
G.A. Dutra ◽  
V.L.T. Jesus ◽  
...  

ABSTRACT The objectives of this study were to characterize the endometritis induced in mares using color Doppler ultrasonography and traditional exams. Experiment 1. Mares (n=20) were submitted to intrauterine inoculation with Escherichia coli. Uterine evaluation was performed at M0 and M1. Experiment 2. Animals were divided into two groups: control group (n=10), and treated group (n=10) using phytotherapeutic solution. In both groups, the uterine evaluation was performed at time T1, T2, and T3. Experiment 3: Uterine evaluation was compared after antibiotic therapy, phytotherapy, and M0. For statistical analysis, the Tukey test, t Student, and Anova test were applied. Experiment 1. The mean values of vascularization at M1 were significantly higher than those obtained at M0 (P<0.05). Bacterial growth was observed in all samples collected. Experiment 2. The mean value of vascularization at time T1 in both groups was significantly higher (P<0.05) compared to M2 and M3. Experiment 3. After antibiotic therapy, the vascularization of the body and uterine horns was not equivalent to the vascularization presented at M0. We can conclude that it was not possible to correlate results obtained by color Doppler ultrasonography with the traditional findings for the diagnosis of endometritis.


Author(s):  
Zhe Pan ◽  
Yanhong Chen ◽  
Tim A. McAllister ◽  
Michael Gänzle ◽  
Graham Plastow ◽  
...  

Shiga toxin (Stx) is the main virulence factor of Shiga toxin-producing Escherichia coli (STEC), and ruminants are the main reservoir of STEC. This study assessed the abundance and expression of Stx genes and the expression of host immune genes, aiming to determine factors affecting these measures and potential gene markers to differentiate Stx gene expression in the recto-anal junction of feedlot beef cattle. Rectal tissue and content samples were collected from 143 feedlot steers of three breeds (Angus, Charolais, and Kinsella Composite) over 2 consecutive years 2014 (n=71) and 2015 (n=72). The abundance and expression of stx1 and stx2 were quantified using qPCR and reverse-transcription-qPCR (RT-qPCR), respectively. Four immune genes (MS4A1, CCL21, CD19, and LTB), previously reported to be down-regulated in super-shedder cattle (i.e., &gt; 104 CFU g-1) were selected, and their expression was evaluated using RT-qPCR. The stx1 gene abundance was only detected in tissue samples collected in year 2 and did not differ among breeds. The stx2 gene was detected in STEC from all samples collected in both years and did not vary among breeds. The abundance of stx1 and stx2 differed (P &lt; 0.001) in content samples collected across breeds (stx1:AN&gt;CH&gt;KC, stx2: AN=CH&gt;KC) in year 1, but not in year 2. Expression of stx2 was detected in 13 RAJ tissue samples (2014: n=6, 2015: n=7), while expression of stx1 was not detected. Correlation analysis showed that the expression of stx2 was negatively correlated with the expression of MS4A1 (R=-0.56, P=0.05) and positively correlated with the expression of LTB (R=0.60, P=0.05). The random forest model and Boruta method revealed that expression of selected immune genes could be predictive indicators of stx2 expression with prediction accuracy of MS4A1 &gt;LTB &gt;CCL21 &gt;CD19. Our results indicate that the abundance of Stx could be affected by cattle breed and sampling year, suggesting that host genetics and environment may influence STEC colonization of the recto-anal junction of feedlot cattle. Additionally, the identified relationship between expressions of host immune genes and stx2 suggests that the host animal may regulate stx2 expression in colonizing STEC through immune functions.


2018 ◽  
Vol 7 (2) ◽  
pp. 35
Author(s):  
Sri Hidanah ◽  
Emy Koestanti Sabdoningrum ◽  
Retno Sri Wahyuni ◽  
Arini Rahmi Dewi ◽  
Erma - Safitri

ABSTRACT               Escherichia coli (E. coli) can be isolated from the environment both inside and outside the hospes body. There were 89 serotypes in which 21% showed resistance to various antibiotics, such as E. coli enterotoxin. Alternative efforts were needed as a substitute for antibiotics, one of them through the use of medicinal plants, such as meniran (Phyllanthus niruri Linn).  Meniran plant is an immunomodulator that serves to repair the immune system of the body. The research was done through several stages: isolation and identification of  E. coli enterotoxin from several broiler farms in East Java using the polymerase chain reaction (PCR) method, E. coli resistance test against some antibiotics, making meniran extract and activation test against E. coli enterotoxin The study was divided into five treatments: T0+ (group of chickens were infected by E. coli enterotoxin), T0- (control group, not infected), T1 (infected by E. coli enterotoksin + 20% meniran extract), T2 (infected by E. coli enterotoksin + 25% extract meniran), T3 (infected by E. coli enterotoxin + 30% extract meniran). Data were analyzed by ANOVA (Analysis of Variance). The results were showed that all of  E. coli DNA isolates which tested by the PCR method was showed positive reactions at 600 bp. In the next stage, that E. coli enterotoxin are resistance to some antibiotics, such as Amoxicillin, Amphicillin, Erythromycin, Cephalosporins, Tetracycline, Cloxacillin and Gentamicin. Furthermore, 30% Phyllanthus niruri linn extract  effective as an antibacterial for the prevention of antibiotic resistance from E. coli enterotoxin. 


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xu Song ◽  
Yi Yang ◽  
Junzhi Li ◽  
Mengxue He ◽  
Yuanfeng Zou ◽  
...  

Abstract Background Enterotoxigenic Escherichia coli (ETEC) is classically associated with acute secretory diarrhea, which induces 2 million people death in developing countries over a year, predominantly children in the first years of life. Previously, tannins (47.75%) were extracted from Galla Chinensis and prepared as Galla Chinensis oral solution (GOS) which showed significant antidiarrheal activity in a castor oil-induced diarrhea in mice. Whether the tannins extract were also effective in treatment of ETEC-induced diarrhea was determined in this study. Methods Mice were randomly divided into 6 groups (n = 22). The mice in the normal and untreated groups were given normal saline. Three GOS-treated groups were received different concentrations of GOS (5, 10 and 15%, respectively) at a dose of 10 mL/kg. Mice in the positive control group were fed with loperamide (10 mg/kg). The treatment with GOS started 3 days before infection with ETEC and continued for 4 consecutive days after infection. On day 3, mice were all infected with one dose of LD50 of ETEC, except those in the normal group. Survival of mice was observed daily and recorded throughout the study. On days 4 and 7, samples were collected from 6 mice in each group. Results GOS could increase the survival rate up to 75%, while in the untreated group it is 43.75%. The body weights of mice treated with 15% GOS were significantly increased on day 7 in comparison with the untreated group and the normal group. GOS-treatment recovered the small intestine coefficient enhanced by ETEC-infection. The diarrhea index of mice treated with GOS was significantly decreased. GOS increased the levels of IgG and sIgA in the terminal ileum and decreased the levels of pro-inflammatory cytokines (IFN-γ, TNF-α, IL-1β, IL-6 and IL-8) in serum. GOS could increase the amount of intestinal probiotics, Lactobacilli and Bifidobacteria. GOS could alleviate colon lesions induced by ETEC-infection. GOS showed higher potency than loperamide. Conclusions GOS could be a promising drug candidate for treating ETEC infections.


2014 ◽  
Vol 84 (1-2) ◽  
pp. 5-11 ◽  
Author(s):  
Eun Y. Jung ◽  
Sung C. Jun ◽  
Un J. Chang ◽  
Hyung J. Suh

Previously, we have found that the addition of L-ascorbic acid to chitosan enhanced the reduction in body weight gain in guinea pigs fed a high-fat diet. We hypothesized that the addition of L-ascorbic acid to chitosan would accelerate the reduction of body weight in humans, similar to the animal model. Overweight subjects administered chitosan with or without L-ascorbic acid for 8 weeks, were assigned to three groups: Control group (N = 26, placebo, vehicle only), Chito group (N = 27, 3 g/day chitosan), and Chito-vita group (N = 27, 3 g/day chitosan plus 2 g/day L-ascorbic acid). The body weights and body mass index (BMI) of the Chito and Chito-vita groups decreased significantly (p < 0.05) compared to the Control group. The BMI of the Chito-vita group decreased significantly compared to the Chito group (Chito: -1.0 kg/m2 vs. Chito-vita: -1.6 kg/m2, p < 0.05). The results showed that the chitosan enhanced reduction of body weight and BMI was accentuated by the addition of L-ascorbic acid. The fat mass, percentage body fat, body circumference, and skinfold thickness in the Chito and Chito-vita groups decreased more than the Control group; however, these parameters were not significantly different between the three groups. Chitosan combined with L-ascorbic acid may be useful for controlling body weight.


2018 ◽  
Vol 32 (1) ◽  
pp. 30-42 ◽  
Author(s):  
Claudia Traunmüller ◽  
Kerstin Gaisbachgrabner ◽  
Helmut Karl Lackner ◽  
Andreas R. Schwerdtfeger

Abstract. In the present paper we investigate whether patients with a clinical diagnosis of burnout show physiological signs of burden across multiple physiological systems referred to as allostatic load (AL). Measures of the sympathetic-adrenergic-medullary (SAM) axis and the hypothalamic-pituitary-adrenal (HPA) axis were assessed. We examined patients who had been diagnosed with burnout by their physicians (n = 32) and were also identified as burnout patients based on their score in the Maslach Burnout Inventory-General Survey (MBI-GS) and compared them with a nonclinical control group (n = 19) with regard to indicators of allostatic load (i.e., ambulatory ECG, nocturnal urinary catecholamines, salivary morning cortisol secretion, blood pressure, and waist-to-hip ratio [WHR]). Contrary to expectations, a higher AL index suggesting elevated load in several of the parameters of the HPA and SAM axes was found in the control group but not in the burnout group. The control group showed higher norepinephrine values, higher blood pressure, higher WHR, higher sympathovagal balance, and lower percentage of cortisol increase within the first hour after awakening as compared to the patient group. Burnout was not associated with AL. Results seem to indicate a discrepancy between self-reported burnout symptoms and psychobiological load.


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