Chemical treatments for increasing the digestibility of cotton straw: 1. Effect of ozone and sodium hydroxide treatments on rumen metabolism and on the digestibility of cell walls and organic matter

1983 ◽  
Vol 100 (2) ◽  
pp. 393-400 ◽  
Author(s):  
D. Ben-Ghedalia ◽  
G. Shefet ◽  
Y. Dror
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Sodium Hydroxide ◽  
Cell Walls ◽  
Volatile Fatty Acids ◽  
Positive Relationship ◽  
Apparent Digestibility ◽  
Total Volatile ◽  
Chemical Treatments ◽  
Rumen Metabolism

SUMMARYThe digestibility and rumen metabolism of diets containing as 50% of their organic matter (OM), cotton straw (CS) untreated, treated with sodium hydroxide and treated with ozone, were studied in sheep cannulated in the rumen and at the duodenum with simple cannulae. The concentration of total volatile fatty acids (VFA) in the rumen of sheep given the ozone and NaOH treatments was higher than in the untreated diet; however, the VFA profiles were not different. The rumen dehydrogenase activity, suggested to reflect general microbial activity, was higher by 83 and 81% in the ozone and NaOH treatments respectively, than in the untreated.Apparent digestibility of organic matter in the ozone-treated diet was 74·6%; 1·25 and 1·17 times higher than in the untreated and NaOH-treated diets respectively. The calculated values for organic matter and cell-wall digestibilities of the cotton straw in the complete diets were: 30·0, 20·0; 60·8, 60·0; and 39·6, 39·7%, respectively, for the untreated, ozone and NaOH-treated cotton straw. Nitrogen metabolism was not impaired by the presence of formic acid in the ozonated cotton straw; the apparent absorption of N from the intestine and the apparent digestibility of N were higher in the ozonetreated diet than in the untreated or NaOH-treated diet.The proportion of organic matter and cell walls digested in the rumen was higher in the NaOH and ozone treatments than in the untreated, and the possible reasons for that are discussed. A positive relationship was found between cell-wall digestion in the rumen (% of intake) and the rate of passage (% per h) of particulate matter from the rumen. The interpretation of this relationship is discussed in general and in view of the results of the present study.

scholarly journals The composition of the cell wall of Fusicoccum amygdali

1971 ◽  
Vol 125 (2) ◽  
pp. 461-471 ◽  
Author(s):  
K. W. Buck ◽  
M. A. Obaidah
Keyword(s):  
Cell Wall ◽  
Sodium Hydroxide ◽  
Cell Walls ◽  
Digestive Enzymes ◽  
Helix Pomatia ◽  
Water Soluble ◽  
Extracellular Polysaccharides ◽  
Lytic Enzymes ◽  
Similar Nature ◽  
Dark Blue

1. The cell wall of Fusicoccum amygdali consisted of polysaccharides (85%), protein (4–6%), lipid (5%) and phosphorus (0.1%). 2. The main carbohydrate constituent was d-glucose; smaller amounts of d-glucosamine, d-galactose, d-mannose, l-rhamnose, xylose and arabinose were also identified, and 16 common amino acids were detected. 3. Chitin, which accounted for most of the cell-wall glucosamine, was isolated in an undegraded form by an enzymic method. Chitosan was not detected, but traces of glucosamine were found in alkali-soluble and water-soluble fractions. 4. Cell walls were stained dark blue by iodine and were attacked by α-amylase, with liberation of glucose, maltose and maltotriose, indicating the existence of chains of α-(1→4)-linked glucopyranose residues. 5. Glucose and gentiobiose were liberated from cell walls by the action of an exo-β-(1→3)-glucanase, giving evidence for both β-(1→3)- and β-(1→6)-glucopyranose linkages. 6. Incubation of cell walls with Helix pomatia digestive enzymes released glucose, N-acetyl-d-glucosamine and a non-diffusible fraction, containing most of the cell-wall galactose, mannose and rhamnose. Part of this fraction was released by incubating cell walls with Pronase; acid hydrolysis yielded galactose 6-phosphate and small amounts of mannose 6-phosphate and glucose 6-phosphate as well as other materials. Extracellular polysaccharides of a similar nature were isolated and may be formed by the action of lytic enzymes on the cell wall. 7. About 30% of the cell wall was resistant to the action of the H. pomatia digestive enzymes; the resistant fraction was shown to be a predominantly α-(1→3)-glucan. 8. Fractionation of the cell-wall complex with 1m-sodium hydroxide gave three principal glucan fractions: fraction BB had [α]D +236° (in 1m-sodium hydroxide) and showed two components on sedimentation analysis; fraction AA2 had [α]D −71° (in 1m-sodium hydroxide) and contained predominantly β-linkages; fraction AA1 had [α]D +40° (in 1m-sodium hydroxide) and may contain both α- and β-linkages.

The effect of different extraction procedures on the recovery of cell walls in forage and faeces from cattle and sheep

1972 ◽  
Vol 78 (3) ◽  
pp. 351-353 ◽  
Author(s):  
K. W. Moir
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Sequential Extraction ◽  
Cell Walls ◽  
Ammonium Oxalate ◽  
Hot Water ◽  
Sodium Sulphite ◽  
Detergent Solution ◽  
Organic Residues ◽  
Cattle And Sheep

SUMMARYForages and faeces from 28 digestibility experiments with cattle or sheep were extracted with neutral detergent solution, with and without sodium sulphite, or sequentially extracted with acid-pepsin, ethyl alcohol, diethyl ether and either ammonium oxalate or hot water. Ammonium oxalate was used to extract small amounts of non-protein material not extracted from legumes by hot water. Compared with sequential extraction the average protein-free organic residues after extraction with detergent, with and without sodium sulphite, were significantly lower in legumes and faeces, but not in grasses. The largest differences occurred in faeces from grasses for which the recoveries of cell walls as percentages of the faecal organic matter were found to be 59·2, 62·0 and 66·4 respectively, after extraction with neutral detergent plus sodium sulphite, neutral detergent alone and sequential extraction with various solvents. It was considered that the differences were due to extraction of cell-wall constituents by both detergent and sulphite.

scholarly journals Effect of Supplementation of Monensin Sodium on rumen metabolism and Milk Yield in Early Lactating Buffalo (Bubalus Bubalus)

2018 ◽  
Vol 14 (2) ◽  
Author(s):  
Prathviraj . ◽  
Shrikant Kulkarni ◽  
N. M. Soren ◽  
Sathisha K. B. ◽  
Srinivas Reddy Bellur ◽  
...  
Keyword(s):  
Milk Yield ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids ◽  
Experimental Period ◽  
Control Group ◽  
Total Volatile ◽  
Rumen Metabolism ◽  
Volatile Fatty Acid Concentration ◽  
Standard Ration ◽  
Sodium Supplementation

The study was carried out to assess the effect of monensin sodium supplementation on rumen fermentation metabolites and milk yield in early lactating buffaloes. Twelve buffaloes in their 2nd week of lactation were selected. Control group was fed on standard ration whereas the treatment group was supplemented with monensin sodium @ 200 mg/head/day in addition to standard ration. Rumen liquor and blood sample was collected at 2nd and 12th week of lactation. Total and individual volatile fatty acids concentration was estimated by gas chromatography. Monensin sodium (P less than 0.05) decreases acetate and increases propionate concentration and decreases the ratio of acetate to propionate in the rumen liquor without altering the total volatile fatty acid concentration in experimental period. Supplemented buffaloes yielded 8.22 per cent more milk than the control.

Optimalisasi Penggunaan Level Sabun Kalsium Minyak Kedelai dalam Ransum Terhadap Karakteristik Fermentasi, Populasi Mikroba dan Kecernaan Nutrien Secara In Vitro Menggunakan Cairan Rumen Sapi Bali

2018 ◽  
Vol 5 (2) ◽  
pp. 11
Author(s):  
Ali Bain ◽  
Komang G. Wiryawan ◽  
Dewi Apri Astuti ◽  
Chairrusyuhur Arman ◽  
Sri Suharti
Keyword(s):  
Fatty Acids ◽  
Organic Matter ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Microbial Population ◽  
Total Volatile ◽  
Calcium Soap ◽  
Total Bacteria ◽  
Range Test

ABSTRAKPenelitian ini bertujuan untuk mengevaluasi optimalisasi penggunaan level sabun kalsium yang berbeda dalam ransum secara in vitro terhadap karakteristik fermentasi, populasi mikroba dan kecernaan nutrien ransum, menggunakan sumber inokulum cairan rumen sapi Bali. Penelitian dilaksanakan menggunakan rancangan acak kelompok dengan 4 jenis perlakuan dalam 3 ulangan.  Ransum penelitian terdiri atas : R1, 40% rumput lapang (RL) + 60% konsentrat (K), R2 (40% RL + 60% K, mengandungn 2.5% SCa-kedelai), R3 (40%  RL + 60% K, mengandung 5% SCa-kedelai), R4 (40%  RL + 60% K, mengandung 7.5% SCa-kedelai). Variabel yang diukur terdiri atas karakteristik fermentasi in vitro (pH, N-NH3, produksi total volatile fatty acids), populasi mikroorganisme (total bakteri dan total protozoa), dan kecernaan nutrien (kecernaan bahan kering dan kecernaan bahan organik). Data dianalisis menggunakan analysis of varians  (ANOVA) dan perbedaan antara perlakuan diuji dengan Duncan’s Multiple Range Test.  Hasil penelitian menunjukkan, penambahan SCa-kedelai pada level yang berbeda dalam konsentrat tidak mempengaruhi pH, konsentrasi amonia, populasi mikroba, kecernaan bahan kering dan kecernaan bahan organik fermentasi tetapi berpengaruh sangat nyata (P<0.002) terhadap produksi total VFA. Produksi total VFA tertinggi diperoleh pada ransum perlakuan R4 (konsentrat yang mengandung 7.5% SCa-kedelai). Produksi total VFA tertinggi diperoleh pada perlakuan R4 dan produksi total VFA paling rendah terjadi pada perlakuan ransum R1 (kontrol).  Produksi total VFA  ransum R1 dan R2 dan ransum R2 dan R3 tidak berbeda nyata namun produksi total VFA ransum R3 lebih tinggi dibanding ransum kontrol. Penambahan SCa-kedelai dalam konsentrat menghasilkan produksi total VFA tetinggi namun mulai menekan populasi total bakteri. Mempertimbangkan hasil peubah fermentasi dan populasi mikroba serta biaya pembuatan produk sabun kalsium, SCa-kedelai pada level 5% merupakan level penggunaan SCa-kedelai yang terbaik dalam ransum.Kata kunci: sabun kalsium, fermentasi, in vitro, kecernaanABSTRACTThe study to evaluate the optimalization the different level of soybean oil calcium soap (CaS-soybean) in ration on in vitro fermentation using Bali cattle rumen fluid. The experiment was arranged in a complete randomized block design with 4 different ration treatments and 3 replicates. Ration treatments were R1: 40% native grass (NG) + 60% concentrate (C), R2 : 40% NG + 60% C, supplemented with 2.5% soybean oil calcium soap (CaS-soybean), and R3 (40% NG + 60% C, supplemented with 5.0% CaS-soybean and R4 (40% NG + 60% C, supplemented with 7.5% CaS-soybean). Variables measured were fermentation characteristics (pH, NH3-N, total volatile fatty acids), microbial population (total bacteria and total protozoa), and nutrient digestibility (dry matter and organic matter digestibility). Data were analyzed using analysis of variance (ANOVA) and the differences between treatments means were examined by Duncan Multiple Range Test. Results of the study  showed that the four different level of CaS-soybean in ration did not have any significant effect (P>0,05) on pH, NH3-N, total bacteria, total protozoa, dry matter and organic matter digestibility. The treatments significantly increased (P<0.05) the production of total volatile fatty acids.  The highest production of total VFA was obtained from ration R4 (concentrate containing 7.5% CaS-soybean) and the lowest was obtained from treatment R1 (control). There were no significant differences between R1 and  R2, and between R2 and R3 on the total VFA production. However, the total productions of VFA in ration R3 were higher than that of the control ration. The addition of CaS-soybean in the concentrate had increased the total production of VFA, but at the same time, it began pressing the total population of bacteria. Considering the results of the fermentation variables and microbial population as well as the cost of making calcium soap products, CaS-soybean at 5% level was selected as the best level of CaS-soybean in ration.Keywords : calcium soap, fermentation, in vitro, digestibility

A source of error in the estimation of the organic matter content of samples containing calcium carbonate

1972 ◽  
Vol 79 (3) ◽  
pp. 541-542 ◽  
Author(s):  
F. D. DeB. Hovell ◽  
E. R. Ørskov
Keyword(s):  
Organic Matter ◽  
Calcium Carbonate ◽  
Volatile Fatty Acids ◽  
Organic Matter Content ◽  
Matter Content ◽  
Carbonate Content ◽  
Apparent Digestibility ◽  
High Calcium ◽  
The Difference

The conventional method for the determination of the organic matter content of diets and faeces is by ashing the sample at 550 °C (A.O.A.C, 1965)Organic matter (O.M.) = dry matter (D.M.)–ash.If the sample contains calcium carbonate, then some of this carbonate will be converted to oxide (Vogel, 1951) and the weight of carbon dioxide evolved will be calculated as organic matter. The amount of carbonate converted will be dependent upon the temperature at which the sample is ashed and the duration of the ashing process. We have found (Ørskov, Hovell & Allen, 1966; Hovell & Greenhalgh, 1972) that when calcium salts of volatile fatty acids are included in diets given to sheep, most of the calcium is excreted in the faeces as carbonate, and since apparent digestibility is the difference between the amounts of nutrient ingested and excreted, the estimation of the apparent digestibility of organic matter will be in error if no correction is made for the high calcium carbonate content of the faeces. For example, a diet which contained 9·2% of calcium acetate had a true organic matterapparent digestibility of 86·8%. Had no correction been made this would have been estimated as 84·9%.

The digestion of pasture plants by sheep. III. The digestion of forage oats varying in maturity and in the content of protein and soluble carbohydrate

1969 ◽  
Vol 20 (2) ◽  
pp. 347 ◽  
Author(s):  
JP Hogan ◽  
RH Weston
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Cell Wall ◽  
Organic Matter ◽  
Volatile Fatty Acids ◽  
Fertilizer Application ◽  
Feed Consumption ◽  
Soluble Carbohydrate ◽  
Fertilizer Treatment ◽  
Soluble Nitrogen

A comparison has been made of the composition, intake, and digestion of forage oats grown with and without the application of nitrogen fertilizer and harvested at three stages of maturity. The chemical composition of the forages showed the usual changes with maturity. Fertilizer application had little effect on the levels of cell wall constituents but, as expected, decreased the levels of soluble carbohydrate and increased those of total nitrogen, alcohol-soluble nitrogen, and nitrate. The digestibility of organic matter, cell wall constituents, and nitrogen declined with advancing maturity, all three parameters being little affected by the fertilizer treatment. Feed consumption declined only with the most mature diet and was not affected by the fertilizer treatment even though the high nitrogen (HN) diets supplied 4–5 g nitrate nitrogen per day and relatively small amounts of soluble carbohydrate. There was a loss of dietary nitrogen from the stomach with the HN diets and a gain with the low nitrogen (LN). The amount of nitrogen in the digesta leaving the stomach per unit intake of nitrogen increased with maturity. The quantities of protein leaving the stomach were too great to be accounted for as microbial protein, and hence appreciable quantities of plant protein must have passed through the stomach. The digestibility of crude protein in the intestines was not affected either by maturity of the forages or by fertilizer treatment. There was little effect of advancing maturity or fertilizer application on: (a) the extent of digestion of organic matter and the structural carbohydrates in the stomach relative to that occurring in the intestines; (b) the proportion of digestible organic matter derived from rumen volatile fatty acids and amino acids; � the potential value of the metabolizable energy from volatile fatty acids and amino acids to provide net energy for fattening; (d) most parameters associated with the movement of digesta through the stomach. Advancing maturity of the diets was associated with increased expenditure of time in chewing activities.

scholarly journals THE EFFECT OF CONCENTRATE ON THE DIGESTION OF BROMEGRASS CONSTITUENTS

1981 ◽  
Vol 61 (4) ◽  
pp. 935-944 ◽  
Author(s):  
K. K. FRANKLIN ◽  
J. E. WINCH ◽  
G. K. MACLEOD
Keyword(s):  
Fatty Acids ◽  
Cell Wall ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Neutral Detergent Fiber ◽  
Total Volatile ◽  
Acid Detergent Fiber ◽  
Concentrate Supplementation ◽  
Wall Components

A nylon bag technique was used to study the effect of concentrate feeding on the rate and extent of digestion of bromegrass constituents in eight fistulated sheep fed rations at or slightly above maintenance, at concentrate to bromegrass ratios of 3:97, 22:78, 44:56 and 66:34. The eight sheep were arranged in a double 4 × 4 latin square design with nylon bag incubation times of 3, 6, 12, 24 and 48 h. Rations containing 44 and 66% concentrate depressed rumen digestion of bromegrass dry matter at both 24- and 48-h incubation times. The extent of digestion depression varied among sheep and was related to the animal's ability to buffer increases in rumen acidity. Digestibilities of neutral detergent fiber, acid detergent fiber, cellulose and hemicellulose followed trends similar to that of dry matter. Rumen osmolality, total volatile fatty acids and ammonia levels appeared to have little relationship to bromegrass dry matter digestion. The apparent or total gut digestibility of cell wall components was not depressed. This suggested that additional digestion took place in the ileum, cecum and colon and that the proportion of concentrate supplementation may affect ruminal but not the total gut utilization of roughage at maintenance levels of intake.

The estimation of the metabolizable energy of forage from its cell-wall content and digestible cell wall

1974 ◽  
Vol 82 (3) ◽  
pp. 423-426 ◽  
Author(s):  
K. W. Moir
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Cell Walls ◽  
Metabolizable Energy ◽  
Published Data ◽  
Energy Value ◽  
Image Position ◽  
The Relationship

SUMMARYPublished data are used in support of a hypothesis that equal amounts of digestible cell walls and digestible non-cell walls do not contribute equally to the energy value of forages. The relationship between the concentration in the organic matter of metabolizable energy (kcal/g organic matter) determined at the maintenance level of feeding, and the percentages of cell wall and digestible cell wall in the forage organic matter is defined by:This equation derived from published data on 12 grasses appears to be applicable to both grasses and legumes.

Effects of supplementing low-quality hay with groundnut foliage and cassava tops on feed intake, apparent digestibility and rumen fermentation in crossbred cattle

2019 ◽  
Vol 59 (9) ◽  
pp. 1660 ◽  
Author(s):  
Pok Samkol ◽  
Keo Sath ◽  
Mikaela Patel ◽  
Mom Seng ◽  
Kjell Holtenius
Keyword(s):  
Organic Matter ◽  
Feed Intake ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Basal Diet ◽  
Rumen Fermentation ◽  
Apparent Digestibility ◽  
Crossbred Cattle ◽  
Manihot Esculenta Crantz ◽  
Arachis Hypogea

Four adult crossbred cattle, fitted with rumen fistula in the dorsal sac, were randomly allocated to four treatments in a 4 × 4 Latin-square design to study the effects of supplementing sun-dried groundnut (Arachis hypogea L.) foliage (DGF) and sun-dried cassava (Manihot esculenta, Crantz) tops (DCT) on feed intake, apparent digestibility and rumen fermentation. All animals were fed a basal diet of rice straw ad libitum and para grass (Brachiaria mutica) at 1% of liveweight (LW). The four experimental diets were as follows: (1) control, basal diet without supplement; (2) DGF, basal diet + DGF; (3) DCT, basal diet + DCT; and (4) DGF+DCT, basal diet + DGF + DCT. The supplementary feeds were fed to the level of 2 g crude protein (CP)/kg LW in Diets 2 and 3 and 1 g CP/kg LW of each feed in Diet 4. The results indicated that total intake of dry matter (DM), organic matter and CP increased with DGF and DGF+DCT diets, while neutral detergent fibre (NDF) intake was highest in the DGF diet. Digestibilities of DM, organic matter, CP and NDF were improved in DGF and DGF+DCT treatments. However, the diet with DCT supplement had lowest CP and NDF digestibility. Ruminal NH3-N and total volatile fatty acids increased in all diets with supplements, but ruminal pH, volatile fatty acid profile and in sacco losses of DM, CP and NDF of sun-dried para grass in the rumen did not differ among the treatments.

scholarly journals Relative Contributions of Bacteria, Protozoa, and Fungi to In Vitro Degradation of Orchard Grass Cell Walls and Their Interactions

2000 ◽  
Vol 66 (9) ◽  
pp. 3807-3813 ◽  
Author(s):  
S. S. Lee ◽  
J. K. Ha ◽  
K.-J. Cheng
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Rumen Fluid ◽  
Wall Material ◽  
P System ◽  
Cell Wall Material ◽  
Cell Wall Degradation ◽  
Chemical Treatments ◽  
System A ◽  
Microbial Groups

ABSTRACT To assess the relative contributions of microbial groups (bacteria, protozoa, and fungi) in rumen fluids to the overall process of plant cell wall digestion in the rumen, representatives of these groups were selected by physical and chemical treatments of whole rumen fluid and used to construct an artificial rumen ecosystem. Physical treatments involved homogenization, centrifugation, filtration, and heat sterilization. Chemical treatments involved the addition of antibiotics and various chemicals to rumen fluid. To evaluate the potential activity and relative contribution to degradation of cell walls by specific microbial groups, the following fractions were prepared: a positive system (whole ruminal fluid), a bacterial (B) system, a protozoal (P) system, a fungal (F) system, and a negative system (cell-free rumen fluid). To assess the interactions between specific microbial fractions, mixed cultures (B+P, B+F, and P+F systems) were also assigned. Patterns of degradation due to the various treatments resulted in three distinct groups of data based on the degradation rate of cell wall material and on cell wall-degrading enzyme activities. The order of degradation was as follows: positive and F systems > B system > negative and P systems. Therefore, fungal activity was responsible for most of the cell wall degradation. Cell wall degradation by the anaerobic bacterial fraction was significantly less than by the fungal fraction, and the protozoal fraction failed to grow under the conditions used. In general, in the mixed culture systems the coculture systems demonstrated a decrease in cellulolysis compared with that of the monoculture systems. When one microbial fraction was associated with another microbial fraction, two types of results were obtained. The protozoal fraction inhibited cellulolysis of cell wall material by both the bacterial and the fungal fractions, while in the coculture between the bacterial fraction and the fungal fraction a synergistic interaction was detected.

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