Extraction, Quantification, and Antioxidant Activities of Phenolics from Pericarp and Seeds of Bitter Melons (Momordica charantia) Harvested at Three Maturity Stages (Immature, Mature, and Ripe)

2010 ◽  
Vol 58 (7) ◽  
pp. 4428-4433 ◽  
Author(s):  
Ronny Horax ◽  
Navam Hettiarachchy ◽  
Pengyin Chen
Keyword(s):  
Antioxidant Activities ◽  
Momordica Charantia ◽  
Maturity Stages

scholarly journals Comparison of Phenols Content and Antioxidant Activity of Fruits from Different Maturity Stages of Ribes stenocarpum Maxim

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3148 ◽  
Author(s):  
Yuwei Wang ◽  
Delin Qi ◽  
Shulin Wang ◽  
Xiaohai Cao ◽  
Ying Ye ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Design Method ◽  
Extraction Process ◽  
Coumaric Acid ◽  
Solvent Concentration ◽  
Box Behnken Design ◽  
Maturity Stages ◽  
Ripening Stages ◽  
Intracellular Ros ◽  
Unripe Fruits

Differences in the content of nine phenols and the antioxidant capacity of Ribes stenocarpum Maxim (RSM) fruits at different stages of maturity were investigated, and the extraction process of polyphenols from RSM was also optimized using Box-Behnken design method. Results showed that the content of the nine phenols varied considerably at different ripening stages; catechin, chlorogenic acid, coumaric acid, and ferulic acid were abundant in immature fruits but decreased with fruit ripening, whereas the levels of rosemary acid and querctin acid were low in immature fruits and increased with time, reaching the highest value after the fruit was completely mature. The phenols extracted from RSM fruits possessed good antioxidant activities for effective and rapid scavenging of DPPH and ABTS free radicals, as well as intracellular ROS. Analysis of the phenols content at different maturity stages indicated that the unripe fruits had significantly higher polyphenols content than mature fruits. Consequently, unripe fruits possessed higher antioxidant activities. According to the overall results of the extraction process optimization, the selected optimal conditions for extracting polyphenols from RSM were as follows: extraction time, 95 min; solvent concentration, 60%; ratio of sample to solvent, 1:25.

scholarly journals EFFECT OF MATURITY STAGES AND SOLVENT POLARITY ON PHENOLIC CONTENT AND ANTIOXIDANT CAPACITY OF GUAVA CULTIVARS

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Surabhi Shukla ◽  
Radha Kushwaha ◽  
Monika Singh ◽  
Jyotsana Singh ◽  
Vinita Puranik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Solvent Polarity ◽  
Total Phenolic ◽  
Maturity Stages ◽  
Ripening Stages

Tropical fruits claim to have phenolic compounds that have been reported to possess strong antioxidant activity. The study investigatedantioxidant activity and total phenolic content of five Guava cultivars namely Allahabad Safeda, Allahabad Surkha, Lalit, Chittidar and Apple color harvested at three fruit ripening stages (unripe, semi-ripe and ripe) and extracted by four solvents (ethanol, methanol, acetone and water) with different polarities (50%, 70% and 100%).The findings suggest that guava cultivars with maturity stages and extracting solvent affected its phenolic content and antioxidant activity significantly. Pure solvents were inefficient extraction medium for antioxidants. Enhanced extraction yields were obtained from solvent containing higher water concentrations and 50% ethanol is a recommended solvent for extracting antioxidants compounds from guava fruit. High correlations between phenolic compositions and antioxidant activities of guava extracts were observed. The total phenolics and antioxidant capacity decreases as the ripening proceeds (unripe>semi-ripe>ripe) in all five cultivars.This study facilitates information on the total phenolic content and antioxidant capacities of guava cultivars which is an important commercial fruit. Results obtained showed that solvent polarities and ripening stages played the major role in extraction of ployphenolics from Guava. The significant declines in the levels of total phenolics and antioxidant capacity during fruit development suggest that the unripe fruits can also be taken for processing into nutraceutical foods.

Antioxidant activities of various extracts and fractions of Sorbus domestica fruits at different maturity stages

2006 ◽  
Vol 98 (4) ◽  
pp. 599-608 ◽  
Author(s):  
Aikaterini Termentzi ◽  
Panagiotis Kefalas ◽  
Eugene Kokkalou
Keyword(s):  
Antioxidant Activities ◽  
Maturity Stages

scholarly journals Antioxidants Capacity, Phenolic and Oxalate Content from Two Varieties of Solanum melongena at Different Maturity Stages

2021 ◽  
pp. 54-63
Author(s):  
A. Mohd Zulkhairi ◽  
M. N. Siti Aisyah ◽  
M. Razali ◽  
G. Nur Syafini ◽  
M. B. Umikalsum ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Agricultural Research ◽  
Antioxidant Activities ◽  
Solanum Melongena ◽  
Mature Stage ◽  
Total Phenolic ◽  
Maturity Stages ◽  
Freeze Dried ◽  
Stage 4 ◽  
Stage 1

Aims: To investigate the antioxidant activities, total phenolic and oxalate contents in two varieties of eggplant (Solanum melongena) (terung telunjuk (TT) and terung rapuh (TR)) at different maturity stages. Study design: Each sample was extracted three times (n=3) for the antioxidant activities, total phenolic and oxalates content. All the data were analysed by using ANOVA and Tukey Pairwise tests. Place and duration of study: Malaysian Agricultural Research and Development Institute (MARDI), between December 2019 and October 2020. Methodology: Two varieties of eggplant (TT and TR) were cultivated and the samples were tagged and the fruits were harvested according to their maturity stages (stage 1 – stage 4). Samples were freeze dried and extracted to evaluate the antioxidant activities as well as the total phenolic and oxalate contents. Results: Total phenolic contents (TPC) in TR were lower from stage 1 to stage 3 but high at over mature stage (stage 4) meanwhile TPC in TT increased upon maturity. The DPPH assay from the fruit extracts of TT in all maturity stages showed a stronger antioxidant activity as compared to TR, in which fruit of TT from stage 3 was double in antioxidant activity as compared to TR. The FRAP assay of both eggplants showed extracts of TT having a higher ferric reduction power in all stages as compared to TR. Meanwhile, both eggplant varieties showed different total, soluble and insoluble oxalate contents in all maturity indices. TT had the highest total oxalate content at stage 1 as compared to TR while the soluble oxalate content increased in TR in all maturity stages. The highest percentage of soluble oxalate content was observed in TR at stage 4 with 95.5%. Conclusion: Phytochemical findings from these eggplant varieties showed their potentials to improve livelihood and public health. More comprehensive studies on the bioactive compounds, structural elucidation and pharmacological evaluation are to be conducted to understand the possible effects of these phytochemical results.

scholarly journals Comparison of Bioactive Compounds and Antioxidant Activities of Maclura tricuspidata Fruit Extracts at Different Maturity Stages

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 567 ◽  
Author(s):  
Dae-Woon Kim ◽  
Won-Jae Lee ◽  
Yoseph Asmelash Gebru ◽  
Han-Seok Choi ◽  
Soo-Hwan Yeo ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Antioxidant Properties ◽  
Polyphenolic Compounds ◽  
Total Carotenoids ◽  
Food Material ◽  
Maturity Stages ◽  
Flight Mass Spectrometry ◽  
First Time

Abstract: Maclura tricuspidata fruit contains various bioactive compounds and has traditionally been used in folk medicine and as valuable food material in Korea. The composition and contents of bioactive compounds in the fruit can be influenced by its maturity stages. In this study, total phenol, total flavonoid, individual polyphenolic compounds, total carotenoids and antioxidant activities at four maturity stages of the fruit were determined. Polyphenolic compounds were analyzed using high-pressure liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) and HPLC. Among 18 polyphenolic compounds identified in this study, five parishin derivatives (gastrodin, parishin A, B, C, E) were positively identified for the first time in this plant. These compounds were also validated and quantified using authentic standards. Parishin A was the most abundant component, followed by chlorogenic acid, gastrodin, eriodictyol glucoside, parishin C, parishin E and parishin B. The contents of all the polyphenolic compounds were higher at the immature and premature stages than at fully mature and overmature stages, while total carotenoid was found to be higher in the mature and overmature stages. Overall antioxidant activities by three different assays (DPPH, ABTS, FRAP) decreased as maturation progressed. Antioxidant properties of the fruit extract are suggested to be attributed to the polyphenols.

scholarly journals Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda lucida and Momordica charantia Leaves from Benin

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 434 ◽  
Author(s):  
Michaelle Chokki ◽  
Mihaela Cudălbeanu ◽  
Cheikna Zongo ◽  
Durand Dah-Nouvlessounon ◽  
Ioana Otilia Ghinea ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Momordica Charantia ◽  
Antidiabetic Activity ◽  
Array Detector ◽  
Scavenging Activity ◽  
Inhibition Assay ◽  
Medicinal Uses

Background: Momordica charantia Linn. (Cucurbitaceae), the wild variety of bitter melon and Morinda lucida Benth (Rubiaceae) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of M. charantia and M. lucida leaves and their antidiabetic activity. Methods: Antioxidant activities were evaluated by micro-dilution technique using DPPH free radical scavenging activity and β-carotene-linoleate bleaching assay. The α-amylase inhibition assay was carried out utilizing the 3,5-dinitrosalicylic acid procedure, while β-glucosidase inhibition assay was demonstrated using as substrate p-nitrophenyl-β-D-glucopyranoside (PNPG). HPLC-DAD analysis was realized using a high-performance liquid chromatography systems with diode-array detector, L-3000. Results: Chlorogenic acid, epicatechin, daidzein, rutin, naringin, quercetin, naringenin and genistein were identified as polyphenol compounds in the both plants extract. Dichloromethane and ethyl acetate extracts showed a good α-amylase inhibitory activity (56.46 ± 1.96% and 58.76 ± 2.74% respectively). M. lucida methanolic extract has shown IC50 of 0.51 ± 0.01 mg/mL, which is the lowest for DPPH scavenging activity. M. lucida dichloromethane extract showed the highest inhibitory capacity of β-glucosidase activity (82.11. ± 2.15%). Conclusion: These results justify some traditional medicinal uses of both plants. The purified fractions could be used in future formulations, possibly incorporated in functional foods to combat certain diseases.

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