Impact of Cymbopogon flexuosus (Poaceae) essential oil and primary components on the eclosion and larval development of Aedes aegypti

AbstractThe current study describes the effects of sub-lethal concentrations and constituent compounds (citral and geranyl acetate) of Cymbopogon flexuosus essential oil (EO) on the development of Aedes aegypti. We treated eggs with 6, 18, or 30 mg L−1 and larvae with 3 or 6 mg L−1 of EO and its major compounds (citral and geranyl acetate). Citral and geranyl acetate were evaluated at 18, 30, and 42 mg L−1 and compared with commercial growth inhibitors (diflubenzuron and methoprene). We measured larval head diameter, siphon length, and larval length. Finally, we examined concentrations of molt hormone (MH) and juvenile hormone III (JH III) using high-performance liquid chromatography coupled to mass spectrometry. All geranyl acetate concentrations decreased egg hatching, while EO altered molting among larval instars and between larvae and pupae, with an increase in the larval length (3 mg L−1: 6 ± 0.0 mm; 6 mg L−1: 6 ± 0.7 mm) and head width (3 mg L−1: 0.8 ± 0 mm; 6 mg L−1: 0.8 ± 0.0 mm) compared with the control group. We did not detect chromatographic signals of MH and JH III in larvae treated with C. flexuosus EO or their major compounds. The sub-lethal concentrations C. flexuosus EO caused a similar effect to diflubenzuron, namely decreased hormone concentrations, an extended larval period, and death.

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Impact of Cymbopogon Flexuosus (Poaceae) Essential Oil and Primary Components on the Eclosion and Larval Development of Aedes Aegypti

10.21203/rs.3.rs-388057/v1 ◽

2021 ◽

Author(s):

Ruth Mariela Castillo-Morales ◽

Sugey Ortiz Serrano ◽

Adriana Lisseth Rodríguez Villamizar ◽

Stelia Carolina Méndez-Sánchez ◽

Jonny Edward Duque

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

High Performance ◽

Geranyl Acetate ◽

Control Group ◽

Head Width ◽

Growth Inhibitors ◽

Egg Hatching ◽

Cymbopogon Flexuosus ◽

Moult Hormone

Abstract The current study describes the effects of sub-lethal concentrations and constituent compounds (citral and geranyl acetate) of Cymbopogon flexuosus essential oil (EO) on the development of Aedes aegypti. We treated eggs with 6, 18, and 30 mg.L-1 and larvae with 3 and 6 mg.L-1 EO concentrations. Citral and geranyl acetate were evaluated at 18, 30, and 42 mg.L-1 and compared to the commercial growth inhibitors (diflubenzuron and methoprene). We measured larval head diameter, siphon length, and body length. Finally, we examined concentrations of moult hormone (MH) and juvenile hormone III (JH III) using high-performance liquid chromatography coupled to mass spectrometry. The EO decreased egg hatching at all concentrations and altered molting among larval instars and between larvae and pupae, with an increase in the length (3 mg.L-1: 6 ± 0.0 mm; 6 mg.L-1: 6 ± 0.7 mm) and head width (3 mg.L-1: 0.8 ± 0 mm; 6 mg.L-1: 0.8 ± 0.0 mm) compared with the control group. We did not detect chromatographic signals of MH and JH III in larvae treated with C. flexuosus EO or their major compounds. The sub-lethal concentrations C. flexuosus EO caused a similar effect to diflubenzuron, decreasing hormone concentration, extending the larval period, and death.

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Impact of Cymbopogon flexuosus (Poaceae) essential oil and primary components on the eclosion and larval development of Aedes aegypti (Diptera: Culicidae)

10.1101/2020.12.30.424851 ◽

2021 ◽

Author(s):

Ruth Mariela Castillo-Morales ◽

Sugey Ortiz Serrano ◽

Adriana Lisseth Rodríguez Villamizar ◽

Stelia Carolina Mendez-Sanchez ◽

Jonny E. Duque

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

High Performance ◽

Morphological Analysis ◽

Geranyl Acetate ◽

Control Group ◽

Head Width ◽

Egg Hatching ◽

Cymbopogon Flexuosus ◽

Eggs And Larvae

AbstractThe current study describes the effects of sub-lethal concentrations and constituent compounds (citral and geranyl acetate) of Cymbopogon flexuosus essential oil (EO) on the development of Aedes aegypti (L.) eggs and larvae. To demonstrate the ovicidal activity of EO, we treated embryonated eggs with 6, 18, and 30 mg.L-1 and larvae with 3 and 6 mg.L-1 EO concentrations. Citral and geranyl acetate were evaluated at 18, 30, and 42 mg.L-1 and compared with commercial growth inhibitors (diflubenzuron and methoprene) at 3 and 6 mg.L-1 concentrations. For each treatment, we measured larval head diameter, siphon length, and body length. To complement the morphological analysis, we examined concentrations of moult hormone (MH) and juvenile hormone III (JH III) using high-performance liquid chromatography (HPLC) coupled to mass spectrometry. The EO decreased egg hatching at all concentrations: 6 mg.L-1 in 45.3%; 18 mg.L-1 in 23.3%, and 30 mg.L-1 in 34.6%. EO also altered molting among larval instars and between larvae and pupae, with an increase in the length (3 mg.L-1: 6 ± 0.0 mm; 6 mg.L-1: 6 ± 0.7 mm) and head width (3 mg.L-1: 0.8 ± 0 mm; 6 mg.L-1: 0.8 ± 0.0 mm) compared with the control group (length: 5.3 ± 0 mm; head width: 0.7 ± 0.0 mm). We did not detect chromatographic signals of MH and JH III in larvae treated with C. flexuosus EO or their major compounds. The sub-lethal concentrations C. flexuosus EO caused a similar effect to diflubenzuron, decreasing hormone concentration, extending the larval period, and death.

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Anthelmintic activity of Cymbopogon citratus against Haemonchus contortus

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612015059 ◽

2015 ◽

Vol 24 (3) ◽

pp. 268-275 ◽

Cited By ~ 14

Author(s):

Iara Tersia Freitas Macedo ◽

Lorena Mayana Beserra de Oliveira ◽

Wesley Lyeverton Correia Ribeiro ◽

Jessica Maria Leite dos Santos ◽

Kaline das Chagas Silva ◽

...

Keyword(s):

Essential Oil ◽

Haemonchus Contortus ◽

Anthelmintic Activity ◽

Meriones Unguiculatus ◽

Parasitic Nematodes ◽

Control Group ◽

Cymbopogon Citratus ◽

Egg Hatching

Parasitic nematodes are of major economic importance in livestock. An alternative for the control of parasites is phytotherapy. This study evaluated the efficacy of Cymbopogon citratus decoction (CcD), C. citratus essential oil (CcEo) and citral against Haemonchus contortus using in vitro egg hatch test (EHT) and larval development test (LDT) and an in vivo test using a Meriones unguiculatus (gerbil) model. The effect of 800 mg/kg CcEo was evaluated in gerbils that had been artificially infected with 5,000 third-stage H. contortus larvae. The effective concentrations required to inhibit 50% (EC50) of egg hatching were 0.46, 0.14 and 0.13 mg/mL for CcD, CcEo and citral, respectively. The EC50 values in the LDT were 5.04, 1.92 and 1.37 mg/mL for CcD, CcEo and citral, respectively. H. contortus population in the group treated with C. citratus essential oil was reduced by 38.5% (P< 0.05) in comparison to the control group. These results suggest that it may be possible to use C. citratusessential oil to control of H. contortus parasite of small ruminant.

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Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0009587 ◽

2021 ◽

Vol 15 (7) ◽

pp. e0009587

Author(s):

Ivana Sierra ◽

Jose Manuel Latorre-Estivalis ◽

Lucila Traverso ◽

Paula V. Gonzalez ◽

Ariel Aptekmann ◽

...

Keyword(s):

Molecular Docking ◽

Essential Oil ◽

Aedes Aegypti ◽

Vector Control ◽

Gene Families ◽

Control Group ◽

Mosquito Vector ◽

Rna Seq ◽

Chemosensory Proteins ◽

The Impact

Background Aedes aegypti (L.) is an urban mosquito, vector of several arboviruses that cause severe diseases in hundreds of million people each year. The resistance to synthetic insecticides developed by Ae. aegypti populations worldwide has contributed to failures in vector control campaigns, increasing the impact of arbovirus diseases. In this context, plant-derived essential oils with larvicidal activity could be an attractive alternative for vector control. However, the mode of action and the detoxificant response of mosquitoes to plant derived compounds have not been established, impairing the optimization of their use. Methods and findings Here we compare gene expression in Ae. aegypti larvae after 14 hrs of exposure to Eucalyptus camaldulensis essential oil with a control group exposed to vehicle (acetone) for the same lapse, by using RNA-Seq. We found differentially expressed genes encoding for cuticle proteins, fatty-acid synthesis, membrane transporters and detoxificant related gene families (i.e. heat shock proteins, cytochromes P450, glutathione transferases, UDP-glycosyltransferases and ABC transporters). Finally, our RNA-Seq and molecular docking results provide evidence pointing to a central involvement of chemosensory proteins in the detoxificant response in mosquitoes. Conclusions and significance Our work contributes to the understanding of the physiological response of Ae. aegypti larvae to an intoxication with a natural toxic distilled from Eucalyptus leafs. The results suggest an involvement of most of the gene families associated to detoxification of xenobiotics in insects. Noteworthy, this work provides important information regarding the implication of chemosensory proteins in the detoxification of a natural larvicide. Understanding the mode of detoxification of Eucalyptus distilled compounds could contribute to their implementation as a tool in mosquito control.

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PERBEDAAN KEMAMPUAN DAYA TOLAK MINYAK ATSIRI BUNGA MELATI (Jasminum sambac) DAN DAUN SElASIH (Ocimum basilicum) SEBAGAI REPElEN NYAMUK Aedes aegypti

GEMA LINGKUNGAN KESEHATAN ◽

10.36568/kesling.v11i1.178 ◽

2013 ◽

Vol 11 (1) ◽

Author(s):

Meilina Yuhanita Dewi ◽

Koerniasari . ◽

Irwan Sulistyo

Keyword(s):

Infectious Disease ◽

Essential Oil ◽

Aedes Aegypti ◽

Ocimum Basilicum ◽

Control Group ◽

Human Hand ◽

Significant Difference ◽

Quasi Experimental ◽

Randomized Control ◽

Jasminum Sambac

Dengue fever is an infectious disease caused by dengue virus and is transmitted by Aedesaegypti and Aedes albopictus. In the year 2010 there had been 150.000 cases in Indonesia and thenumber of deaths was 1317. Transmission of this disease can be prevented using plant essentialoils to be applied as repellents. This study was aimed at investigating the differences betweenjasmine essential oil (Jasminum sambac) and leaf basil (Ocimum basilicum) as repellents againstAedes aegypti mosquito.This is a quasi-experimental study using a non-randomized control group pretest-posttestdesign. Subjects were female Aedes aegypti mosquitoes, 25 mosquitoes on each test. Thematerials used were jasmine flowers essential oil at 40% concentration and basil leaves essentialoil at 90% concentration. As much as 10 replications were performed during the study.Independent SamplesT Test was carried out for analyzing the data.The average number of mosquitoes that came in contact with human hand that had beengiven jasmine flowers essential oil at 40% concentration were 3 mosquitos and basil leavesessential oil at 90% concentration was one mosquito. There was no significant difference betweenthe number of Aedes aegypti mosquitoes that come in contact with human hand applied withjasmine flowers essential oil at 40% concentration and basil leaves essential oil at 90%concentration (p =0.000; 0=0. 05).The conclusion was 90% concentration of essential oils of basil leaves, having 3.32%linalool as an active ingredient had a better ability as a repellent against Aedes aegypti comparedto jasmine flowers essential oil at 40% concentration which has 2.9% linalool as an activeingredient. Essential oil of basil leaves can be applied as a substitute for synthetic chemicalrepellent topically every 8.5 hours.Keywords: jasmine essential oil, basil leaf; Aedes aegypti

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EFEKTIVITAS TANAMAN SEREH WANGI SEBAGAI LARVASIDA NYAMUK Aedes aegypti

Vektora Jurnal Vektor dan Reservoir Penyakit ◽

10.22435/vk.v13i1.3767 ◽

2021 ◽

Vol 13 (1) ◽

pp. 19-26

Author(s):

Achmad Farich ◽

Agung Aji Perdana ◽

Dian Yunita

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Fourth Instar ◽

Test Material ◽

Control Group ◽

Composition Analysis ◽

Control Group Design ◽

Breeding Sites ◽

Group Design ◽

Post Test

Dengue Haemorrhagic Fever is an infectious disease caused by the dengue virus which is transmitted mainly through the bite of the Aedes aegypti mosquito. Currently, larvicides are an important strategy in dengue vector control. Larvisides are applied to mosquito breeding sites to kill larvae. This study aims to determine the effectiveness of citronella essential oil as a larvicide for Aedes aegypti. An experimental design with a randomized post-test only control group design was applied in this study using the first to fourth instar larvae of Aedes aegypti as the test material. The concentrations of essential oils applied were 10 ppm, 50 ppm, 100 ppm, 500 ppm and control with 4 replications for 3 different days. A total of 25 Ae aegypti larvae were used in each treatment and observed for 24 hours. Data analysis was performed using univariate and bivariate (probit test and Kruskal Wallis test). The results showed that all concentrations in the treatment group had significant differences with the control group with p < 0.05. This laboratory test also revealed that citronella essential oil was effective to kill the fourth instar larvae of Aedes aegypti with an LC50 of 1.553 mg/L. Citronella essential oil was effective in killing 50% of IVth instar larvae (LT50) within 3.6 minutes. The results of the chemical composition analysis of citronella essential oil using GC-MS showed citronellal, 2,6-octadien-1-ol, 3,7-dimethyl, and citronellol as the most abundant compositions. Citronella essential oil has potential as an effective botanical pesticide to control Aedes aegypti larvae. Abstrak Demam Berdarah Dengue adalah salah satu penyakit menular yang disebabkan virus dengue yang ditularkan dari seseorang ke orang lain melalui nyamuk Aedes aegypti. Saat ini, larvasida merupakan salah satu strategi penting dalam pengendalian vektor dengue. Tujuan dari penelitian ini adalah untuk melihat efektivitas minyak atsiri sereh wangi sebagai larvasida Aedes aegypti. Metode yang digunakan berupa eksperimental design dengan rancangan randomized post test only control group design dengan menggunakan larva Ae. aegypti instar I sampai IV sebagai material uji. Konsentrasi minyak atsiri yang digunakan yaitu 10 ppm, 50 ppm, 100 ppm, 500 ppm dan kontrol dengan empat kali pengulangan selama tiga hari berbeda. Masing-masing perlakuan menggunakan 25 larva dan dilakukan pengamatan selama 24 jam. Analisis data dilakukan secara univariat dan bivariat (uji probit dan uji kruskal wallis). Hasil penelitian menunjukkan bahwa semua konsentrasi pada kelompok perlakuan memiliki perbedaan secara bermakna dengan kelompok kontrol p<0,05. Minyak atsiri sereh wangi pada semua konsentrasi perlakuan efektif dalam membunuh larva Ae. aegypti. Konsentrasi minyak atsiri sereh wangi yang paling baik membunuh 50% (LC50) larva Ae. aegypti instar IV sebesar 1,553 mg/L. Minyak atsiri sereh wangi mampu membunuh larva Ae. aegypti instar IV sebanyak 50% (LT50) pada waktu 3,616 menit. Komponen utama minyak sereh atsiri sereh wangi yang teridentifikasi dengan GC-MS adalah citronellal, 2,6-octadien-1-ol, 3,7-dimetil, dan citronellol. Minyak atsiri sereh wangi dapat berpotensi sebagai larvasida botani yang efektif untuk mengontrol nyamuk Ae. aegypti

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UJI EFEKTIFITAS FORMULA SPRAY DARI MINYAK ATSIRI HERBA KEMANGI (Ocimum Sanctum L ) SEBAGAI REPELLENT NYAMUK Aedes aegypti

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v2i2.66 ◽

2017 ◽

Vol 2 (2) ◽

pp. 189

Author(s):

Resmi Aini ◽

Rina Widiastuti ◽

Nuha Afra Nadhifa

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Essential Oils ◽

Negative Control ◽

Repellent Activity ◽

Ocimum Sanctum ◽

Control Group ◽

Control Group Design ◽

A Value ◽

Post Test

Dengue fever is transmitted by the bite of Aedes aegypti mosquitoes infected with denguevirus. Preparations antinyamuk much use of harmful chemicals such as DEET (diethylmetatoluamide) and permethrin that can lead to pollution of the environment, leavingno residue and cause resistance to the drug. To determine the activity of essential oils from herbs basil (Ocimum Sanctum L) in the preparation of spray as a mosquito repellent and determine the effective concentrationof the spray.This type of research is True experiment with design Post Test Only Control Group Design. Repellent activity was observed by means of spray is sprayed on the back of the hand probandus, then tested on female mosquitoes with the introduction into the cage containing20 mosquitoes each treatment. Spray essential oils showed repellent activity of Aedes aegypti with a value of correlation r = 0.964 higher concentration of essential oils the fewer mosquitoes that land. Spray herbal essential oil of basil (Ocimum basilicum L) has a repellent activitycompared to the negative control. The effectiveness of the herb basil essential oil sprayobtainedat 74.131 ppm lC50 and lC90 of 775.711 ppm

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Integrated Control of Aedes Aegypti With Parasitic Nematodes, Biological Control Agents, Chemical Insecticides and Plant Essential Oil

10.21203/rs.3.rs-1068544/v1 ◽

2021 ◽

Author(s):

Jinfeng Xiong ◽

Hui Zhang ◽

Caixia Li ◽

Rui Ma ◽

Hui Ai ◽

...

Keyword(s):

Biological Control ◽

Essential Oil ◽

Aedes Aegypti ◽

High Efficiency ◽

Pcr Analysis ◽

Control Group ◽

Mosquito Vector ◽

Biological Control Agents ◽

Competitive Binding Assay ◽

Density Control

Abstract Aedes aegypti can transmit dengue fever, yellow fever, Chikungunya fever, Zika virus disease and vector density control is the most effective way to prevent these infectious diseases. However, the extensive use of chemical pesticides has caused a series of problems, such as environmental pollution, killing non-target organisms and so on. In this study, a parasitic nematode, Romanomermis wuchangensis was used in the larviciding evaluation of Ae. aegypti, while the activity of four chemical insecticides and biological control agents were tested. Besides, Mentha haplocalyx essential oil was isolated and its olfactory physiological function with OBP1 protein of Ae. aegypti antenna was measured by the prokaryotic expression and fluorescence competitive binding assay. Compared with the control group, R. wuchangensis indicated high efficiency and environmental friendliness in the control of Ae. aegypti. After the second instar larvae were parasitized, the mortality of two treatment groups exceeded 75%. Compared to control group, the quantitative real-time PCR analysis results demonstrated that SOD, POD and CAT genes had obvious high expression levels in the nematodes parasitic groups. The antioxidant enzyme test results also exhibited obvious difference of SOD, CAT and POD during the nematode parasitic period. Besides, Bacillus thuringiensis (Bti) and chemical insecticide experimental results also showed great insecticidal efficacy against mosquito larvae. Five chemical components including Menthol, Pinene, Limonene, Isopulegol and Pulegone were identified from M. haplocalyx and exhibited great binding ability with AaegOBP1 protein. Present results illustrated that the integrated application of these various mosquito vector control methods in the future has broad prospects.

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Ateleia glazioveana and Ocimum basilicum: plants with potential larvicidal and repellent against Aedes aegypti (Diptera, Culicidae)

Research Society and Development ◽

10.33448/rsd-v10i17.24733 ◽

2021 ◽

Vol 10 (17) ◽

pp. e228101724733

Author(s):

Kelly Alievi ◽

Giovana Tamara Capoani ◽

Maike Buzatto ◽

Daniela Miorando ◽

Patrícia Zanotelli Serpa ◽

...

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Negative Control Group ◽

Negative Control ◽

Ocimum Basilicum ◽

Repellent Activity ◽

Control Group ◽

Bacillus Thuringiensis Israelensis ◽

Synthetic Compounds ◽

Positive Controls

The Aedes aegypti is the main vector of the transmission of diseases such as dengue, yellow fever, Chikungunya, and Zika. The agents used for the control of mosquito involve the use of synthetic compounds, which may cause toxicity and contamination environmental. Aiming to test the effectiveness of natural products, the aim of this study was to evaluate the larvicidal and repellent activity of extracts of Ateleia glazioveana Baill. (timbó) and essential oil of Ocimum basilicum L. (manjericão), against A. aegypti. The activities of hydroalcoholic (HEA) and dichloromethane (DEA) extracts from A. glazioveana against A. aegypti larvae were assessed after exposure for 24, 48, and 72 h, using water and Bacillus thuringiensis israelensis as negative and positive controls, respectively. For repellent evaluation, we investigated the sting attempts of A. aegypti in response to the application of HEA- and DEA-based body creams (5%), as well as an alcoholic spray based on the essential oil from O. basilicum. The repellency of these preparations was compared with negative (vehicle) and positive (DEET) controls. Although HEA showed no apparent larvicidal activity, DEA at concentrations of 150 to 500 mg/ml showed high larvicidal potential after 24 h, compared to the negative control group (p < 0.001). Moreover, the DEA-based cream and the alcoholic spray of O. basilicum based essential oil showed higher repellency compared with the vehicle (84.5% and 70.5%, respectively). Accordingly, it is possible to infer that extracts and preparations of A. glazioveana and O. basilicum exhibit larvicidal and repellent activity against A. aegypti.

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