Amorphous apatite thin film formation on a biodegradable Mg alloy for bone regeneration: strategy, characterization, biodegradation, and in vitro cell study

Bioactive films with a nanoplate structure were prepared on the surface of a biodegradable AZ31B magnesium (Mg) alloy via anodization in simulated body fluid (SBF) as an electrolyte to control Mg biodegradability and improve surface bioactivity.

Download Full-text

CaCO3 thin-film formation mediated by a synthetic protein-lysozyme coacervate

RSC Advances ◽

10.1039/c6ra28808a ◽

2017 ◽

Vol 7 (25) ◽

pp. 15302-15308

Author(s):

Chaeyeon Son ◽

Sun Young Kim ◽

So Yeong Bahn ◽

Hyung Joon Cha ◽

Yoo Seong Choi

Keyword(s):

Thin Film ◽

Film Formation ◽

Immiscible Liquid ◽

Complex Coacervation ◽

Liquid Phases ◽

Synthetic Protein ◽

Thin Film Formation

A thin film was formed through in vitro CaCO3 crystallization in the presence of complex coacervates, which was expected to be planar and poorly crystalline CaCO3 guided at the interface of two immiscible liquid phases upon complex coacervation.

Download Full-text

Article Commentary: A Commentary on “Evaluation of the in vitro Bioactivity of Bioceramics”

Bone and Tissue Regeneration Insights ◽

10.4137/btri.s4128 ◽

2010 ◽

Vol 3 ◽

pp. BTRI.S4128 ◽

Cited By ~ 2

Author(s):

Y.H. Hsu ◽

I.G. Turner ◽

A.W. Miles

Keyword(s):

Cell Culture ◽

Simulated Body Fluid ◽

Bone Regeneration ◽

Tissue Regeneration ◽

Body Fluid ◽

In Vitro Bioactivity ◽

Interesting Article ◽

Screening Efficiency ◽

Selection Of

The interesting article reported in Bone and Tissue Regeneration Insights by Wu and Xiao entitled “Evaluation of the in vitro bioactivity of bioceramics” contrasts two methods (Simulated body fluid (SBF) and cell culture experiments) which have been commonly used to evaluate the in vitro bioactivity of bioceramics. Limitations in estimating the bioactivity of bioceramics using both methods have been reviewed. Therefore, Wu and Xiao suggest the combination of these two methods to evaluate the bioactivity of bioceramics can improve the screening efficiency for the selection of bioactive ceramics for bone regeneration.

Download Full-text

In Vitro Degradation of Ultrafine Grained Mg-Zn-Ca Alloy by High-Pressure Torsion in Simulated Body Fluid

Materials Science Forum ◽

10.4028/www.scientific.net/msf.706-709.504 ◽

2012 ◽

Vol 706-709 ◽

pp. 504-509 ◽

Cited By ~ 2

Author(s):

Shao Kang Guan ◽

Zhen Wei Ren ◽

Jun Heng Gao ◽

Yu Feng Sun ◽

Shi Jie Zhu ◽

...

Keyword(s):

High Pressure ◽

Simulated Body Fluid ◽

Body Fluid ◽

High Pressure Torsion ◽

Cast Alloy ◽

Mg Alloy ◽

Second Phase ◽

Ultrafine Grained ◽

Degradation Properties

In this paper the in vitro degradation of ultrafine grained (UFG) Mg-Zn-Ca alloy produced by HPT was investigated by electrochemical measurements and immersion tests in SBF. It was found that UFG Mg alloy had better degradation properties and also higher microhardness value than as-cast Mg alloy. The corrosion current density of UFG Mg alloy decreased by about two orders of magnitude, compared with that of as-cast alloy. Through electrochemical impedance spectroscopy (EIS) test，UFG Mg alloy showed a higher charge transfer resistance value. In immersion test, UFG Mg alloy in SBF exhibited more uniform corrosion and lower degradation rate (0.0763 mm/yr) than as-cast alloy. The degradation properties were related with the microstructure evolution, namely the grain refinement and redistribution of second phase. Keywords: Mg-Zn-Ca alloy; High-pressure torsion (HPT); Degradation behavior; Simulated body fluid (SBF); Microhardness

Download Full-text

G0600-8-4 ZnO Thin Film Formation on Mg Alloy Surface with Radio Frequency Oxygen Plasma

The proceedings of the JSME annual meeting ◽

10.1299/jsmemecjo.2010.3.0_207 ◽

2010 ◽

Vol 2010.3 (0) ◽

pp. 207-208

Author(s):

Keisuke Shoji ◽

Masaya Sugimoto

Keyword(s):

Thin Film ◽

Radio Frequency ◽

Oxygen Plasma ◽

Film Formation ◽

Mg Alloy ◽

Alloy Surface ◽

Zno Thin Film ◽

Thin Film Formation

Download Full-text

IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

Journal of industrial technology ◽

10.21908/jit.2015.8 ◽

2015 ◽

Vol 23 (1) ◽

pp. 1-14

Author(s):

Sudirman Sahid ◽

◽

Nor Shahida Kader Bashah ◽

Salina Sabudin ◽

◽

...

Keyword(s):

Calcium Phosphate ◽

Simulated Body Fluid ◽

Body Fluid ◽

Biphasic Calcium Phosphate ◽

Silica Content

Download Full-text

Tetracalcium Phosphate/Monetite/Calcium Sulfate Hemihdrate Biocement Powder Mixtures Prepared by the One-Step Synthesis for Preparation of Nanocrystalline Hydroxyapatite Biocement-Properties and In Vitro Evaluation

Materials ◽

10.3390/ma14092137 ◽

2021 ◽

Vol 14 (9) ◽

pp. 2137

Author(s):

Lubomir Medvecky ◽

Maria Giretova ◽

Radoslava Stulajterova ◽

Lenka Luptakova ◽

Tibor Sopcak

Keyword(s):

Simulated Body Fluid ◽

Body Fluid ◽

Calcium Sulfate ◽

Powder Mixtures ◽

Liquid Component ◽

Tetracalcium Phosphate ◽

Nanocrystalline Hydroxyapatite ◽

One Step ◽

Calcium Sulfate Hemihydrate

A modified one-step process was used to prepare tetracalcium phosphate/monetite/calcium sulfate hemihydrate powder cement mixtures (CAS). The procedure allowed the formation of monetite and calcium sulfate hemihydrate (CSH) in the form of nanoparticles. It was hypothesized that the presence of nanoCSH in small amounts enhances the in vitro bioactivity of CAS cement in relation to osteogenic gene markers in mesenchymal stem cells (MSCs). The CAS powder mixtures with 15 and 5 wt.% CSH were prepared by milling powder tetracalcium phosphate in an ethanolic solution of both orthophosphoric and sulfuric acids. The CAS cements had short setting times (around 5 min). The fast setting of the cement samples after the addition of the liquid component (water solution of NaH2PO4) was due to the partial formation of calcium sulfate dihydrate and hydroxyapatite before soaking in SBF with a small change in the original phase composition in cement powder samples after milling. Nanocrystalline hydroxyapatite biocement was produced by soaking of cement samples after setting in simulated body fluid (SBF). The fast release of calcium ions from CAS5 cement, as well as a small rise in the pH of SBF during soaking, were demonstrated. After soaking in SBF for 7 days, the final product of the cement transformation was nanocrystalline hydroxyapatite. The compressive strength of the cement samples (up to 30 MPa) after soaking in simulated body fluid (SBF) was comparable to that of bone. Real time polymerase chain reaction (RT-PCR) analysis revealed statistically significant higher gene expressions of alkaline phosphatase (ALP), osteonectin (ON) and osteopontin (OP) in cells cultured for 14 days in CAS5 extract compared to CSH-free cement. The addition of a small amount of nanoCSH (5 wt.%) to the tetracalcium phosphate (TTCP)/monetite cement mixture significantly promoted the over expression of osteogenic markers in MSCs. The prepared CAS powder mixture with its enhanced bioactivity can be used for bone defect treatment and has good potential for bone healing.

Download Full-text

Preparation and Degradation Characteristics of MAO/APS Composite Bio-Coating in Simulated Body Fluid

Coatings ◽

10.3390/coatings11060667 ◽

2021 ◽

Vol 11 (6) ◽

pp. 667

Author(s):

Zexin Wang ◽

Fei Ye ◽

Liangyu Chen ◽

Weigang Lv ◽

Zhengyi Zhang ◽

...

Keyword(s):

Simulated Body Fluid ◽

Composite Coating ◽

Body Fluid ◽

Composite Coatings ◽

Degradation Process ◽

Ceramic Coatings ◽

Immersion Test ◽

Substrate Material ◽

Atmospheric Plasma

In this work, ZK60 magnesium alloy was employed as a substrate material to produce ceramic coatings, containing Ca and P, by micro-arc oxidation (MAO). Atmospheric plasma spraying (APS) was used to prepare the hydroxyapatite layer (HA) on the MAO coating to obtain a composite coating for better biological activity. The coatings were examined by various means including an X-ray diffractometer, a scanning electron microscope and an energy spectrometer. Meanwhile, an electrochemical examination, immersion test and tensile test were used to evaluate the in vitro performance of the composite coatings. The results showed that the composite coating has a better corrosion resistance. In addition, this work proposed a degradation model of the composite coating in the simulated body fluid immersion test. This model explains the degradation process of the MAO/APS coating in SBF.

Download Full-text