Mechanochemical Treatment of Quercetin and Curcumin to Obtain Eutectic Mixtures with High Antioxidant Activity

CrystEngComm ◽  
2021 ◽  
Author(s):  
Richard Fernando D'Vries ◽  
Valentina Renza-Diaz ◽  
Martin emilio Gonzalez ◽  
Kriss Dayhana Pantoja
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Biological Systems ◽  
Mechanochemical Treatment ◽  
Natural Antioxidant ◽  
Eutectic Mixtures ◽  
High Antioxidant Activity ◽  
The Creation

Quercetin and curcumin are well-known natural antioxidant molecules used to prevent the malignant effect caused by free radicals on biological systems. This work addressed the creation of eutectic mixtures from...

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SPONS Stylissa sp. DENGAN MENGGUNAKAN METODE DPPH (1,1-difenil-2-pikrilhidrazil)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 419
Author(s):  
Samuel I.M. Sibarani ◽  
Adithya Yudistira ◽  
Deby A. Mpila
Keyword(s):  
Antioxidant Activity ◽  
Cell Wall ◽  
Free Radicals ◽  
Ethanol Extract ◽  
Dna Bases ◽  
Oxidation Reactions ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
High Antioxidant Activity ◽  
Ethanol Extracts

ABSTRACTHabitat of sponge Stylissa sp., were under the sea and these sponges contain active compounds, which are more active than the compounds produced by terrestrial plants. Antioxidants are inhibitors of oxidation reactions due to the free radicals, which can cause weak damage to unsaturated cells, cell wall membranes, blood vessels, DNA bases, and lipid tissue, that causing disease. The study was conducted to determine the antioxidant activity of ethanol extracts  of sponge Stylissa sp., which is located on the Bangka Island, Likupang District, North Minahasa Regency. Sponge Stylissa sp., was extracted by maceration with using ethanol. Testing of antioxidant activity was carried out by the DPPH method measured by a UV-Vis spectrophotometer. The results of the study showed that ethanol extracts of sponge Stylissa sp., has antioxidant activity in each concentration and the highest at a concentration of 100 mg / L. The conclusion is a ethanol extract of sponge Stylissa sp. have high antioxidant activity with a concentration of 25 mg/L (77,40%), concentration of 50 mg/L (85,63%), concentration of 75 mg/L (88,66%), and concentration 100 mg/L (88,96%). Key words: Stylissa sp. Sponge, Antioxidant, DPPH (1,1-diphenyl-2-picrylhydrazyl) ABSTRAKHabitat dari spons Stylissa sp. terdapat di bawah laut dan spons ini mengandung senyawa aktif yang persentase keaktifannya lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Antioksidan adalah zat penghambat reaksi oksidasi akibat radikal bebas yang dapat menyebabkan kerusakan lemah tak jenuh, membran dinding sel, pembuluh darah, basa DNA, dan jaringan lipid sehingga menimbulkan penyakit. Penelitian dilakukan untuk mengetahui aktivitas antioksidan ekstrak etanol biota laut spons Stylissa sp. yang terdapat di pulau Bangka, Kecamatan Likupang, Kabupaten Minahasa Utara. Spons Stylissa sp. ini diekstrak dengan metode maserasi menggunakan etanol. Pengujian aktivitas antioksidan ini dilakukan dengan metode DPPH yang diukur dengan alat spektrofotometer UV-Vis. Hasil dari penelitian menunjukan bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan disetiap konsentrasi dan yang tertinggi pada konsentrasi 100 mg/L. Kesimpulan yang didapat bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan yang tinggi dengan konsentrasi 25 mg/L (77,40%), konsentrasi 50 mg/L (85,63%), konsentrasi 75 mg/L (88,66%), dan konsentrasi 100 mg/L (88,96%). Kata Kunci : Spons Stylissa sp., Antioksidan, DPPH (1,1-difenil-2-pikrilhidrazil)

scholarly journals FORMULATION OF EFFERVESCENT GRANULES FROM RED GINGER (ZINGIBERIS OFFICINALE ROSCOE VAR. RUBRUM) EXTRACT AND ITS ANTIOXIDANT ACTIVITY

2022 ◽  
pp. 112-115
Author(s):  
DIAH LIA AULIFA ◽  
DIKI PRAYUGO WIBOWO ◽  
NENI SAFITRI ◽  
ARIF BUDIMAN
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Physical Stability ◽  
Angle Of Repose ◽  
Wet Granulation ◽  
Flow Properties ◽  
Organoleptic Evaluation ◽  
Hausner Ratio ◽  
High Antioxidant Activity ◽  
Red Ginger

Objective: Ginger is one of the Indonesian plants that has been used as traditional medicine. The flavonoids and phenols compounds contained high antioxidant activity. This study aimed to formulate effervescent granules (EG) from red ginger (RG) extract and evaluate its antioxidant activity. Methods: The formulation of EG from RG extract was prepared by the wet granulation method using different concentrations of polyvinylpyrrolidone (PVP). Furthermore, the flowability of granules was evaluated, including flow rate, angle of repose, bulk density, tapped density, Carr's index, Hausner ratio, and effervescent time. The physical stability of granules such as organoleptic evaluation, effervescent time, and pH measurement was also evaluated after 28 d of storage, and the antioxidant activity of EG from RG extract was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH). Results: The result showed that the EG of RG extract was successfully prepared by wet granulation with a concentration of 15%. In addition, the flowability study showed that all formulas of EG from RG extract have good flow properties, and the granules showed excellent flow properties based on Carr′s index results. The effervescent time of granules remained within the acceptable range according to USP, and the physical stability did not change even after 28 d of storage. The IC50 of EG from RG extract was 283.28±3.6 ppm and has moderate in free radicals scavenging activity. Conclusion: EG from RG extract can be used as food supplements to protect the human body from free radicals and inhibit oxidases.

scholarly journals Potensi Antioksidan Alami pada Ekstrak Kulit Buah Jamblang (Syzigium cumini (L.) Skeels) Menggunakan Metode DPPH (The Potency of Natural Antioxidant in The Rind Extract of Jamblang (Syzigium cumini (L.) Skeels) using DPPH Method)

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Ayu Nirmala Sari ◽  
Kusdianti Kusdianti ◽  
Diky Setya Diningrat
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
The Body ◽  
Natural Antioxidant ◽  
Negative Effects ◽  
Ic50 Value ◽  
Dpph Method ◽  
Antioxidant Potency

AbstrakStress oksidatif pada tubuh dapat memicu berbagai penyakit. Stress oksidatif ini disebabkan karena radikal bebas berlebih. Tubuh memerlukan antioksidan untuk mengurangi pengaruh radikal bebas dan meredam dampak negatifnya. Penelitian ini bertujuan untuk mengevaluasi daya antioksidan ekstrak kulit buah jamblang (Syzigium cumini (L.) Skeels) dengan menggunakan metode DPPH. Berdasarkan hasil pengujian daya antioksidan ekstrak kulit buah jamblang diketahui bahwa ekstrak kulit buah jamblang memiliki kandungan antioksidan yang tergolong aktivitas sedang dengan nilai IC50 sebesar 169.3. Hasil penelitian ini menunjukkan bahwa kulit buah jamblang berpotensi untuk dikembangkan sebagai sumber antioksidan alami bagi manusia.Kata Kunci : antioksidan, DPPH, ekstraksi, jamblang, radikal bebas, stress oksidatif AbstractOxidative stress can induce many diseases. Oxidative stress is caused by excessive free radicals in the body. The body required antioxidant to decrease and hush the negative effects of free radicals. The aim of this study was to evaluate the antioxidant potency of extract rind of jamblang (Syzigium cumini (L.) Skeels) using DPPH method. The result showed that the jamblang rind extract had moderate antioxidant activity and the IC50 value was 169.3.  Based on this result, jamblang rind was potential to be natural antioxidant for human.Keyword: antioxidant, DPPH, extraction, jamblang, free radicals, oxidative stress

scholarly journals Antioxidant potentials of polyphenolic extracts from leaves of trees and fruit bushes

2011 ◽  
Vol 34 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Sylwia Cyboran ◽  
Dorota Bonarska-Kujawa ◽  
Ireneusz Kapusta ◽  
Jan Oszmiański ◽  
Halina Kleszczyńska
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Membrane Lipids ◽  
Natural Antioxidants ◽  
Erythrocyte Ghosts ◽  
Quercetin Derivatives ◽  
Apple Leaves ◽  
High Antioxidant Activity

Antioxidant potentials of polyphenolic extracts from leaves of trees and fruit bushesThe aim of the work was to determine the antioxidant potential of extracts from leaves of strawberry, blackcurrant and apple in relation to lipids contained in the erythrocyte membrane. The studies performed have shown that the substances used protect membrane lipids against oxidation, clearly reducing the level of free radicals in erythrocyte ghosts suspension. The antioxidant activity of the substances studied follows the sequence: strawberry leaves > apple leaves > blackcurrant leaves. The results of the research on the antioxidant activity when confronted with the contents of polyphenols in the extracts indicates that the antioxidant potentials of the extracts depend both on the quantity and kind of individual polyphenols; in particular, on the kind and quantity of quercetin derivatives that constitute over 60 % of all the phenolic compounds. Moreover, the high antioxidant activity of the extracts may be also due to other, nonphenolic substances that occur in leaves. The extracts exhibit very good properties as free radical scavenges, and can thus be used as cheap, easily available, natural antioxidants in the industries where natural antioxidants in the form of fruit extracts have been used for long.

scholarly journals Potential of β-carotene as anti-aging serum: A narrative review

2021 ◽  
Vol 12 (3) ◽  
pp. 1999-2004
Author(s):  
Nadhiya Nurfitra Rahmah ◽  
Nabilla Dira Tama ◽  
Nenis Rahma Wulandari ◽  
Waras Nurcholis
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
The Body ◽  
Premature Aging ◽  
Literature Study ◽  
Natural Sources ◽  
Antioxidant Mechanism ◽  
Low Concentrations ◽  
High Antioxidant Activity ◽  
Β Carotene

β-Carotene is known to have antioxidant activity, the ability to scavenge free radicals and reduce oxidative stress that occurs in the body. However, researchers have paid a little attention to the potential of β-Carotene as an anti-aging agent. The aim of this study was to describe the potential of β-Carotene from natural sources as anti-aging agent via antioxidant mechanism. Literature study was conducted on 28 publications associated with natural sources of β-Carotene, mechanism of anti-aging and antioxidant in the body. The results showed that the best antioxidant activity of β-carotene in scavenging free radicals was at low concentrations while taking into account antioxidant activity, dose, and half-life. UV exposure and air pollution can be a source of free radicals that cause aging of the skin. High antioxidant activity of β-carotene has the potential as anti-aging agent in the form of a beauty serum to treat premature aging of the skin.

scholarly journals Approach to Determination a Structure – Antioxidant Activity Relationship of Selected Common Terpenoids Evaluated by ABTS•+ Radical Cation Assay

2018 ◽  
Vol 13 (3) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Karolina A. Wojtunik-Kulesza ◽  
Łukasz M. Cieśla ◽  
Monika Waksmundzka-Hajnos
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Radical Cation ◽  
Biological Activities ◽  
Double Bonds ◽  
Structural Element ◽  
Antioxidant Mechanism ◽  
High Antioxidant Activity ◽  
Chain Breaking ◽  
Relationship Of

Data available in the literature indicated various biological activities of terpenes. One of the most frequently investigated activities is the ability to scavenge free radicals. To date, studies based on the DPPH• method indicated conjugated double bonds as moieties responsible for antioxidant activity of selected terpenes. In order to verify this theory, studies based on 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) were performed. The spectrophotometric investigations explicitly revealed relatively high antioxidant activity of terpenes possessing conjugated double bonds in comparison with substances without this structural element. It has been shown that the chain-breaking antioxidant activity of terpenes strictly depends on π bonds. TEAC values and percentage scavenging of the free radicals revealed that the most promising antioxidants are α-terpinene, ocimene, pulegone and farnesene. In order to explain the reaction between terpenes and ABTS•+ free radical cation, a possible antioxidant mechanism has been proposed.

scholarly journals Kandungan dan Aktivitas Antioksidan pada Rumput Laut Merah

2021 ◽  
Vol 3 (1) ◽  
pp. 113
Author(s):  
Rafaela E. M. Loho ◽  
Murniati Tiho ◽  
Youla A. Assa
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Literature Review ◽  
The Body ◽  
Premature Aging ◽  
Google Scholar ◽  
Antioxidant Compounds ◽  
Red Seaweed ◽  
High Antioxidant Activity ◽  
Eucheuma Cottonii

Abstract: The imbalance between free radicals and endogenous antioxidants produced by the body can cause cell damage resulting in various diseases such as cancer, heart disease, cataracts, premature aging, and other degenerative diseases. Antioxidants that can capture and neutralize free radicals are needed to cease further reactions causing oxidative stress. Therefore, the consumption of natural antioxidants which are abundant in plants needs to be increased. Red seaweed, known as red algae, has a lot of biological activity compared to other types of seaweed. This study was aimed to determine the content and antioxidant activity of red seaweed (Eucheuma cottonii). This was a literature review study using three databases: Pubmed, ClinicalKey, and Google Scholar. The keywords used were Antioxidant AND Rhodophyta AND Gracilaria sp AND Eucheuma Cottonii. After screening and feasibility assessed we obtained 10 journals using experimental method to evaluate the total phenol content and antioxidant activity of Gracilaria sp. and Eucheuma cottonii. The results showed that red seaweed contained antioxidant compounds such as phenolics and flavonoids that had high antioxidant activity. In conclusion, red seaweed contains compounds with high antioxidant activity such as phenolics and flavonoids. Keywords: antioxidant; red seaweed; phenolic; flavonoid  Abstrak: Ketidakseimbangan jumlah radikal bebas dengan jumlah antioksidan endogen yang diproduksi tubuh dapat menyebabkan terjadinya kerusakan sel yang dapat menimbulkan berbagai penyakit seperti kanker, jantung, katarak, penuaan dini, serta penyakit degeneratif lainnya. Tubuh memerlukan asupan senyawa antioksidan yang mampu menangkap dan menetralisir radikal bebas tersebut, sehingga reaksi lanjutan yang menyebabkan terjadinya stres oksidatif dapat berhenti. Konsumsi antioksidan alami yang banyak terkandung pada tumbuhan perlu ditingkatkan. Rumput laut merah yang dikenal sebagai alga merah merupakan jenis rumput laut yang banyak memiliki aktivitas biologi dibandingkan dengan jenis rumput laut lainnya. Penelitian ini bertujuan untuk melihat kandungan dan aktivitas antioksidan pada rumput laut merah (Eucheuma cottonii). Jenis penelitian ialah suatu literature review dengan pencarian data menggunakan 3 database yaitu PubMed, ClinicalKey dan Google Scholar. Kata kunci yang digunakan ialah Antioksidan AND Rhodophyta AND Gracilaria sp AND Eucheuma Cottonii. Setelah skrining serta uji kelayakan didapatkan 10 jurnal dengan metode penelitian eksperimental yang menguji kandungan total fenol dan aktivitas antioksidan pada Gracilaria sp. dan Eucheuma cottonii. Hasil kajian menunjukkan adanya senyawa yang berperan sebagai antioksidan seperti fenolik dan flavonoid yang memiliki aktivitas antioksidan tinggi. Simpulan penelitian ini ialah rumput laut merah mengandung senyawa yang memiliki aktivitas antioksidan yang tinggi seperti fenolik dan flavonoid.Kata kunci: antioksidan; rumput laut merah; fenolik; flavonoid  

scholarly journals Aktivitas Antioksidan dan Fitokimia dari Ekstrak Etil Asetat Pucuk Idat (Cratoxylum glaucum)

2018 ◽  
Vol 5 (2) ◽  
pp. 69-74 ◽  
Author(s):  
Robby Gus Mahardika ◽  
Occa Roanisca
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Natural Antioxidant ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidants ◽  
Synthetic Antioxidant ◽  
Phytochemical Content

Neutralizing free radicals can use both synthetic and natural antioxidant compounds. Although synthetic antioxidant compounds are more active, but lately the use of synthetic antioxidants is being reduced because they are reported to have carcinogenic side effects. Natural antioxidant compounds found in plants are phenolic groups such as flavonoids, tannins, xanthones, and anthraquinones. This compound is widely found in the genus Cratoxylum where one of the species is pucuk idat (Cratoxylum glaucum). Pucuk idat are often used by people of Bangka as a flavoring dish and are believed to be traditional medicine to facilitate breastfeeding, tighten skin, treat fever, cough, and diarrhea. The increasing of antioxidants needs and lots efficacy of pucuk idat, hence this study aims to determine the antioxidant activity of ethyl acetate extract Cratoxylum glaucum. The antioxidant test in this study used the DPPH (Diphenylpicrylhydrazyl) method and vitamin C as a positive control. The results of this study indicate that the extract of ethyl acetate Cratoxylum glaucum has strong antioxidant activity with value IC50 32,213 μg / mL. Phytochemical content itself includes hydroquinone phenols (tannins), flavonoids, and steroids. This shows that ethyl acetate extract Cratoxylum glaucum can be used as natural antioxidant.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

UJI IMUNOSTIMULATOR DARI KOMBINASI EKSTRAK ETANOL HERBA BINARA (ARTEMISIA VULGARIS LINN) DAN EKSTRAK ETANOL DAUN PUCUK MERAH (SYZYGIUM OLEANA) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT PADA TIKUS JANTAN

2019 ◽  
Vol 1 (2) ◽  
pp. 22-26
Author(s):  
Romauli Anna Teresia Marbun ◽  
Aminah Syarifuddin ◽  
Montysory Silalahi ◽  
Radika Bella Fista Ginting
Keyword(s):  
Antioxidant Activity ◽  
Immune System ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Positive Control ◽  
Artemisia Vulgaris ◽  
Chemical Screening ◽  
Chemical Content ◽  
High Antioxidant Activity ◽  
New Treatments

Diseases mediated by the immune system are difficult problems to treat such as human immunodeficiency virus (HIV) and other lethal viruses. Infections that occur in normal people are generally brief and rarely leave permanent damage. Treatment of this disease requires an aggressive and innovative approach to the development of new treatments so that it requires the role of immunomodulators to improve the immune system. A substance that acts as an enhancer or immune enhancer can be obtained by using herbs that are efficacious as immunostimulants. One of the herbs used is herbal binara (Artemisia vulgaris L) which has been studied as a potential immunomodulator with high antioxidant activity. Previous research also stated that red shoots (Syzygium oleana) were studied as potential immunomodulators with high antioxidant activity. Several other species such as Syzygium samarangense have 16 flavonoida compounds which show pharmacological immunological activity. The purpose of this study was to determine the content of secondary metabolites of ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) and to determine the best dose of extract from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) can reduce the volume of swelling of mouse feet. Examination of the chemical content of secondary metabolites from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) is carried out by chemical screening and characterization of simplicia and extract. The method used is the slow type hypersensitivity method. In this test the independent variable is the secondary metabolite of ethanol extract of herb binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) with four concentrations (50, 100, 200 and 400 mg / kgBB). The positive control used by Stimuno dose is 32.5 mg / kgBB

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