scholarly journals The Effect of Cloud Ear Fungus (Auricularia polytricha) on Serum Total Cholesterol, LDL And HDL Levels on Wistar Rats Induced by Reused Cooking Oil

2018 ◽  
Vol 31 ◽  
pp. 06006
Author(s):  
Ratih Budinastiti ◽  
Henna Rya Sunoko ◽  
Nyoman Suci Widiastiti
Keyword(s):  
Total Cholesterol ◽  
Wistar Rats ◽  
Mean Value ◽  
Negative Control ◽  
Serum Total Cholesterol ◽  
Control Group ◽  
Standard Diet ◽  
Control Group Design ◽  
Cooking Oil ◽  
The Impact

The usage of reused cooking oil affects the increase of serum total cholesterol (TC) and LDL, also the decrease of serum HDL. This condition escalates the risk of atherosclerosis, which could lead to the incidence of cardiovascular disease. Cloud ear fungus is a natural antioxidant that contains polysaccharides, flavonoids, niacin, and vitamin C, which can improve the lipid profiles. Objective of this research is to analyze the impact of water from boiled cloud ear fungus on total cholesterol, LDL, and HDL level of Wistar rats that have been given reused cooking oil. This study is a true experimental research with post test only control group design, using 12 weeks-aged male Wistar rats (n = 24) that were randomly divided into 4 groups. K1 as the negative control, K2 was given reused cooking oil and standard diet, K3 was given water from boiled cloud ear fungus and standard diet, and K4 was given reused cooking oil, water from boiled cloud ear fungus and standard diet. Serum total cholesterol, LDL, and HDL levels were measured by the CHOD-PAP method after 28 days treatment. The study showed that TC mean value of K1 (80.2217 ± 3.61 mg / dL), K2 (195.8483 ± 5.47 mg / dL), K3 (75.5800 ± 4.02 mg / dL), and K4 (110.8683 ± 5.82 mg / dL); p = 0.000. LDL mean value of K1 (29.9200 ± 1.53 mg / dL), K2 (78.4167 ± 1.77 mg / dL), K3 (24.3167 ± 1.77 mg / dL), and K4 (40, 1617 ± 2.84 mg / dL); p = 0.000. HDL mean value of K1 (65.8950 ± 1.99 mg / dL), K2 (24.3233 ± 1.44 mg / dL), K3 (73.2300 ± 1.92 mg / dL), and K4 (54, 9550 ± 2.04 mg / dL); p= 0.000.Conclusion:Water from boiled cloud ear fungus decreases the serum total cholesterol and LDL, 06006 increases serum HDL levels of Wistar rats that has been given reused cooking oil.

scholarly journals Administration of red rosella’s petals extract (Hibiscus sabdariffa) increase level of superoxide dismutase (SOD) wistar rats which induced by repeteadly heated cooking oil

2017 ◽  
Vol 4 (2) ◽  
pp. 13 ◽  
Author(s):  
Novera Herdiani ◽  
Bambang Wirjatmadi ◽  
Merryana Adriani
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Wistar Rats ◽  
Saturated Fatty Acids ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Standard Diet ◽  
Cooking Oil

Background:  repeatedly heated cooking oil is cooking oil that is used many times, had saturated fatty acids containing free radicals. Red rosella petals extract contains antioxidants to minimize the effects of repeatedly heated cooking oil that can raise the levels of SOD. The aim of this study was to analyzed the effect of red rosella’s petals extract against oxidative stress of Wistar rats were induced by repeatedly heated cooking oil with examine levels of  serum SOD.This research was conducted True Experimental Laboratory with a completely randomized design (CRD) and use the post test. The sample consisted of 24 male rats were divided 4 groups: negative control (fed with standard diet); positive control (fed with standard diet + administered by repeatedly heated cooking oil as much as 2.1 ml / kg bw); treatment group dose I (fed with standard diet + administered by red rosella extract dose 540 mg / kg bw + repeatedly heated cooking oil as much as 2.1 ml / kg bw), and treatment group dose II (fed with standard diet + administered by red rosella extract dose 810 mg / kg bw + repeatedly heated cooking oil as much as 2.1 ml / kg bw). Analysis of data using statistical test One Way ANOVA and Tukey HSD (α = 0.05).Results: There were significant differences (p = 0.000) between groups. Red rosella extract treatment group dose 810 mg / kg bw and 540 mg / kg bw were significantly different from the positive control (p = 0.000). Red rosella extract group dose 540 mg / kg bw was significantly different from the negative control (p = 0.000). Red rosella extract group dosage of 810 mg / kg bw was not differ significantly from the control group (p = 0.171).Conclusion: The red rosella’s petals extract dose of 810 mg / kg bw and dose of 540 mg / kg bw were able to prevent oxidative stress. Optimal deose of red rosella petals extract dose of 810 mg / kg bw was the most effective, because the higher antioxidant activity (67.33%) at dose 810 mg / kg bw dose than 540 mg / kg bw.

scholarly journals Difference in Level of Malondialdehyde, Total Cholesterol, and Triglyceride After Administration of Passion Fruit Seed’s Ethanol Extract in Wistar Rats

2019 ◽  
Author(s):  
Dina Keumala Sari ◽  
Marianne Marianne ◽  
Sri Lestari ◽  
Lidya Imelda Laksmi
Keyword(s):  
Total Cholesterol ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Negative Control ◽  
Passion Fruit ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Preclinical Trial ◽  
Significant Difference

High oxidative stress in cells due to inflammation process or excessive cell proliferation would produce oxidants or free radicals with biomarkers, one of which is malondialdehyde (MDA). Passion fruit seed’s contain high antioxidant and are expected to decrease the level of cholesterol and MDA. The objective is to identify the effect of passion fruit seed’s ethanol extract in Wistar rats that have been fed with atherogenic feed. The method was preclinical trial (post-test control group design) in rats, by administering passion fruit seed’s ethanol extract for 14 days. This study used 26 male rats aged two months, divided into 5 groups. The result showed significant difference in MDA level which was found in group that was given passion fruit seed extract 10mg/kg BW with positive control group that was given standard feed. Passion fruit seed’s extract showed significant difference in level of triglyceride, which was found in negative control group that was given atherogenic feed with group that was given passion fruit seed’s extract 5mg/kg BW (mean±standard deviation: 1.09±0.30 mg/dL vs 0.77±0.25mg/dL; p=0.048). This study showed that passion fruit seed’s ethanol extract had significant lowering effect in level of MDA, total cholesterol, and triglyceride for 14 days.

scholarly journals Honey improves lipid profile of diet-induced hypercholesterolemic rats

2016 ◽  
Vol 34 (3) ◽  
pp. 177 ◽  
Author(s):  
Titis Nurmasitoh ◽  
Miranti Dewi Pramaningtyas
Keyword(s):  
Total Cholesterol ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Cholesterol Oxidase ◽  
Negative Control ◽  
Blood Cholesterol ◽  
Control Group ◽  
Blood Levels ◽  
High Fat ◽  
Control Group Design

Background<br />Coronary heart disease (CHD) is a major cause of morbidity and mortality throughout the world, including Indonesia. One of the risk factors for CHD is hypercholesterolemia. One of the natural products that has been developed for the treatment of hypercholesterolemia is honey. Honey contains fructooligosaccharides, various vitamins, minerals, and enzymes which are supposedly able to lower blood cholesterol levels. This research aimed to study the influence of honey on the levels of blood total cholesterol, triglyceride, and low density lipoprotein (LDL) levels in Wistar rats. <br /><br />Methods<br />This study was of experimental post test control group design. Twenty-four male Wistar rats (Rattus norvegicus) were randomly divided into 4 groups. K1 was the negative control group (with normal diet), K2 was the positive control group (with high-fat diet), P1 was fed a high-fat diet for 7 days, followed by high-fat diet plus honey for the next 7 days. P2 was fed a high-fat diet for 7 days, followed by regular diet plus honey for the next 7 days. After completion of this treatment, total cholesterol, triglycerides, and LDL levels were measured by the cholesterol oxidase phenol+aminophenazone (CHOD-PAP) method using enzymatic spectrophotometry principles. <br /><br />Results<br />There were significant differences in total cholesterol, triglyceride, and LDL levels between all groups after day 15 (p&lt;0.05). <br /><br />Conclusion<br />Honey supplementation was able to reduce the blood levels of total cholesterol, triglycerides, and LDL. Honey supplementation accompanied by non-cholesterol feeds could more effectively lower total cholesterol, triglycerides, and LDL serum levels in Wistar rats.

scholarly journals Effect of Mangosteen’s fruit peel extract toward to total cholesterol, LDL, HDL serum in rats were given by used cooking oil

2017 ◽  
Vol 4 (2) ◽  
pp. 45
Author(s):  
Loo Hariyanto Raharjo ◽  
Monica Monica
Keyword(s):  
Body Weight ◽  
Total Cholesterol ◽  
Waste Cooking Oil ◽  
Control Group ◽  
Fruit Peel ◽  
Control Group Design ◽  
Cooking Oil ◽  
Used Cooking Oil ◽  
Post Test ◽  
Per Oral

Waste cooking oil causes increased level of total cholesterol and LDL and decreased level of HDL serum, which became coronary heart disease. The aim of this study was to evaluate the effect of mangosteen peels extract, which contain alpha-mangostin (xanthone) to decreased level of total cholesterol and LDL and increased level of HDL in rats exposed to waste cooking oil per oral. This study used true experimental designs with randomized post-test only control group design. This study used thirty rats were dividing into six treatment group, which control with Na-CMC 0,5%, control with extract of mangosteen pericarp at dosages of 800 mg/ kg body weight, exposed to waste cooking oil per oral, and exposed to waste cooking oil per oral with extract of mangosteen pericarp at dosages of 600, 800, and 1000 mg/kg body weight. All groups were given treatment for 12 weeks. After 12 weeks, rats were anesthetized with chloroform for collection of blood by cardiac puncture. Analysis of level of total cholesterol , LDL, and HDL serum with spectrophotometer. From Post Hoc test with Least Significant Differences it was concluded that extract of mangosteen peels can decreased level of total cholesterol and LDL and increased level of HDL serum.

scholarly journals Pengaruh Pembelajaran Group Investigation dengan Multi Representasi pada Topik Alat-Alat Optik terhadap Penalaran Ilmiah dan Kemampuan Masalah Siswa SMA

2020 ◽  
Vol 5 (4) ◽  
pp. 486
Author(s):  
Apolonia Delviyanti Putri Marga ◽  
Edi Supriana ◽  
Arif Hidayat
Keyword(s):  
High School Students ◽  
Problem Solving ◽  
Scientific Reasoning ◽  
Learning Model ◽  
Optical Devices ◽  
Mean Value ◽  
Control Group ◽  
School Students ◽  
Control Group Design ◽  
The Impact

<p><strong>Abstract: </strong>The purpose of this study is to determine the impact of group investigation learning model with multi representation on the topic of optical devices on scientific reasoning and <em>problem solving</em> skill. This study were carried out using quasi experimental method with pretest-posttest control group design and two XI MIA classes as sample. The result showed that there were an impact of group investigation learning model with multi representation on the topik of optical devices on scientific reasoning and <em>problem solving</em> skill of high school students. The mean value of scientific reasoning and <em>problem solving</em> ability of experimental class students who learned by group investigation learning model with multi representation is higher than control class students who learned by group investigation learning model.</p><strong>Abstrak</strong>: Penelitian ini bertujuan untuk mengetahui pengaruh pembelajaran <em>group investigation </em>dengan multi representasi pada topik alat-alat optik terhadap kemampuan penalaran ilmiah dan kemampuan <em>problem solving</em> siswa SMA. Metode penelitian yang digunakan adalah eksperimen kuasi dengan desain <em>pretest-posttest control group </em>dengan dua kelas XI MIA sebagai sampel. Hasil penelitian menunjukkan adanya pengaruh pembelajaran <em>group investigation </em>dengan multi representasi pada topik alat-alat optik terhadap kemampuan penalaran ilmiah dan pemecahan masalah siswa SMA. Nilai rerata penalaran ilmiah dan kemampuan <em>problem solving</em> siswa kelas eksperimen yang belajar menggunakan pembelajaran <em>group investigation </em>dengan multi representasi lebih tinggi dari siswa kelas kontrol yang belajar menggunakan pembelajaran <em>group investigation</em><em>. </em>

scholarly journals POTENTIAL EFFECT OF MACRO ALGA Caulerpa sp. AND Gracilaria sp. EXTRACT LOWERING MALONDIALDEHYDE LEVEL OF WISTAR RATS FED HIGH CHOLESTEROL DIET

2017 ◽  
Vol 5 (1) ◽  
pp. 71
Author(s):  
Ketut Srie Marhaeni Julyasih ◽  
I Gede Putu Wirawan
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Design Research ◽  
Wistar Rat ◽  
Negative Control ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Standard Diet ◽  
Cholesterol Diet ◽  
High Cholesterol

Seaweed has potential nutrient content such as carotenoids, vitamins, fatty acids, carbohydrates, minerals, and other essential substances. Carotenoids have important biological functions as an antioxidant, and immunostimulatory which can prevent the disease, anti-inflammatory, anti-stress, anti-aging, and protect the skin from the harmful effects of ultraviolet radiation. Seaweed generally consumed as a vegetable by people in Bali, known as the local name Bulung Boni (Caulerpa spp.) and Bulung Sangu (Gracilaria spp.).. So far there has been no report or results of research on the effects of extract ethanol of Bulung Boni (Caulerpa sp.) and Bulung Sangu (Gracilaria sp.) as an antioxidant that can prevent lipid peroxidation which can be seen in decreased level of MDA in liver tissue or blood plasma. Therefore it is necessary to determine of plasmaMDA level of Wistar rat after fed high cholesterol diet treated with extract ethanol of Caulerpa sp. and Gracillaria sp. This experimental study used completely randomized design. Research using total of 24 Wistar rats divided into six sample groups of equal size, all fed with a diet high in cholesterol especially in negative control. The study consisted of negative control group (standard diet), positive control group (high cholesterol diet), high-cholesterol diet with Caulerpa sp. extract dose of 20 mg and 60 mg/100 g, high cholesterol diet with Gracilaria sp. extract dose of 20 mg and 60 mg/100 g body weight rat per day.The study resulted that rats fed high cholesterol diet with treated extract ethanol Caulerpa sp. and Gracilaria sp. with a dose of 20 mg and 60 mg per 100 g body weight rat / day had plasma MDA level significantly lower (p <0.05) compared with rats fed high cholesterol diet without treated with extract of Caulerpa sp. and Gracilaria sp.

scholarly journals Effect of Hydrogel Epigallocatechin-3-Gallate (EGCG) to the Number of Fibroblast Cell Proliferation in the Perforation of Wistar Rat Tooth Pulp

2020 ◽  
Vol 9 (2) ◽  
pp. 93
Author(s):  
Ira Widjiastuti ◽  
Setyabudi Setyabudi ◽  
Kun Ismiyatin ◽  
Djoko Agus Purwanto ◽  
Tiara Sukmawati
Keyword(s):  
Cell Proliferation ◽  
Calcium Hydroxide ◽  
Wistar Rats ◽  
Radical Scavenging ◽  
Fibroblast Cell ◽  
Negative Control ◽  
Tooth Pulp ◽  
Control Group ◽  
Current Standard ◽  
Control Group Design

Background: Pulpitis can occur because the deep cavity preparation and it causes increasing of NO levels. Perforated teeth require direct pulp capping (DPC) treatment. The current standard DPC material is calcium hydroxide. However, several studies have found weaknesses of calcium hydroxide that can affect the success of DPC treatment and new, more biocompatible materials are needed. Epigallocatechin-3-gallate (EGCG) in green tea has many benefits, including antioxidant, anticolagenase, anticancer, anti-inflammatory and has the ability of radical scavenging to clean NO so that pulp healing can occur better by increasing the number of fibroblast cells that play a role in wound healing. Purpose: To determine the concentration of hydrogel EGCGs that are effective in increasing the number of fibroblast cell proliferation in the dental pulp perforation of Wistar rats. Method: This research is a laboratory experimental study with a randomized post test only control group design. Samples used in the study were 24 male Wistar rats which were divided into four groups, namely the negative control group and the treatment group were given EGCG 60 ppm, 90 ppm, and 120 ppm and were decapitated on the 7th day after treatment. The maxilla and the 1st molar were taken and decalcified, to process the HPA reading with HE staining. Observations were made using a microscope with a magnification of 400x. Results: There were significant differences in the treatment groups with 60 ppm and 90 ppm hydrogel hydrogels on the results of the Oneway ANOVA difference test (p <0.05). Conclusion: The concentration of hydrogel EGCG which is effective in increasing fibroblast cell proliferation is 90 ppm.

scholarly journals The Effect of Hydrogel Aloe vera (Aloe vera (L.) Burm) on the Number of Neutrophil Cells in Aggressive Periodontitis Induced by Aggregatibacter actinomycetemcomitans (In Vivo Study on Wistar Rats)

2021 ◽  
Vol 5 (7) ◽  
pp. 657-663
Author(s):  
Chandra Susanto ◽  
Shieny Lokanata ◽  
Jeni Widya Ningrum
Keyword(s):  
Wistar Rats ◽  
Alveolar Bone ◽  
Aloe Vera ◽  
Negative Control Group ◽  
Aggressive Periodontitis ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Control Group Design

Introduction: Aggressive periodontitis is a periodontal disease that runs rapidly, characterized by gross alveolar bone loss, the disease is unprompted by complaints, not related to local aspects. Objective: The aim of this study was to determine the effect of Aloe vera hydrogel on the number of neutrophil cells in aggressive periodontitis induced by A. actinomycetemcomitans in Wistar rats. Methods: This type of research is a laboratory experimental design with post-test only design with control group design. This study used 24 Wistar rats divided into 6 groups, namely, negative control group (Basic hydrogel), ibuprofen and hydrogel Aloe vera 2.5%, 5%, 10%, 20%. Results: The test results for the mean number of neutrophil cells in the hydrogel aloe vera group of 2.5%, 5%, 10%, 20%, ibuprofen, and negative controls were 120.50 ± 12.92, 78.25 ± 7.37, 47, 25 ± 4.03, 1.00 ± 0.82, 0.50 ± 0.58, and 217.25 ± 62.26. The results of the oneway Anova statistical test obtained p value = 0.001, which means that there is a difference in the number of neutrophils of Wistar rats in all treatment groups. Conclusion: The conclusion of this study there is the effect of giving hydrogel aloe vera a concentration of 2.5%, 5%, 10%, 20% with ibuprofen on Wistar rats induced by aggressive periodontitis.

scholarly journals The effect of meniran herbs extract (Phyllantus niruri) to triglycerides blood level in wistar rats

2012 ◽  
Vol 10 (1) ◽  
pp. 23-27
Author(s):  
JUDO YUSTANTO KAHONO ◽  
KISRINI KISRINI ◽  
YUL MARIYAH
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Total Period ◽  
Group I ◽  
Significant Difference ◽  
Post Test ◽  
Group Ii

Kahono JY, Kisrini, Mariyah Y. 2012. The effect of meniran herbs (Phyllantus niruri) extract to blood triglyceride level in rats. Biofarmasi 10: 23-27. The aim of this experiment was to determine the effect of meniran herbs (Phyllantus niruri L.) extract to blood triglycerides level in Wistar rats (Rattus norvegicus). This experiment was a laboratory experiment with pre and post-test with a control group design. The samples were 30 male Wistar rats with ±200 gram of body weight and ±2 months old. The samples were divided into five groups by using a purposive random sampling technique and each group consisted of six rats. Group I as a negative control, group II as a positive control, and groups III, IV and V as meniran herbs extract groups with first dose, second dose, and third dose. All rats were simultaneously given with hyperlipemic feed. Group II was simultaneously administered with Cholvastin 0.5 mg/200 grams body weight/day. Groups III, IV and V were simultaneously treated with meniran herbs extract at dose of 25 mg, 50 mg and 100 mg/200 grams body weight/day. The total period of this experiment was 21 days. At the day 1st and 21st, blood was collected from rat orbital venous plexus. Serum was analyzed for their pre and post-test blood triglycerides. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by a post-hoc test. The value of p=0.002 (p<0.05) showed there was a significant difference between pre-test and post-test in delta means of blood triglycerides level among groups. Meniran herbs extract had a significant triglycerides lowering effect against blood triglycerides level of Wistar rats. The most potential of meniran herbs extract dose was 100 mg/200 gram body weight/day.

scholarly journals Effect of Orthosiphon stamineus Benth on Hematologic Parameters in Lead Acetat Induced Wistar Rats

2021 ◽  
Vol 15 (7) ◽  
pp. 2048-2050
Author(s):  
DWI Retnoningrum ◽  
Nyoman Suci Widyastiti ◽  
Ariosta .
Keyword(s):  
Platelet Count ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Negative Impact ◽  
Wistar Rat ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Orthosiphon Stamineus ◽  
Significant Difference

Introduction: Lead exposure has a negative impact on health. Lead can caused abnormality hematologic parameters. Orthosiphon stamineus Benth is one of the herbal medicines and known to have antioxidant, anti-inflammatory, and anti-angiogenesis. Objective: This study aims to prove the effect of Orthosiphon stamineus Benth on hematologic parameters in lead acetate induced Wistar rat. Method: The study used posttest only control group design. The samples were 30 male Wistar rats, divided into 5 groups. Normal control group (NC) was given normal saline. Negative control group (NG) was induced by lead acetate 30 mg/kg/day. GT-50, GT-100, GT-200 was induced by lead acetate and orally administered by 50,100, and 200 mg/kg Orthosiphon extract once daily for 14 days respectively. One-way Anova and post hoc LSD were used for statistical analysis. Result: Hemoglobin level and leukocyte count in all group were not different (p=0.56 and p=0.25). There was a significant difference in platelet count between all group (p=0.035). Platelet count in NC group (900 ±171 x103/μl) was significantly higher than NG group (656±201 x103/μl, p=0.016), NG group was significantly lower than GT-50 (869±134 x103/μl, p=0.032), GT-100 (930±162 x103/μl, p=0.008) and GT-200 (938±148 x103/μl, p=0.006) Conclusion: There is a decrement of platelets number in lead acetate exposure. Orthosiphon stamineus Benth may increase platelet counts in lead acetate induced Wistar rat. Keywords: Orthosiphon stamineus Benth, hemoglobin, leukocytes, platelets

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