scholarly journals Effect of Mangosteen’s fruit peel extract toward to total cholesterol, LDL, HDL serum in rats were given by used cooking oil

2017 ◽  
Vol 4 (2) ◽  
pp. 45
Author(s):  
Loo Hariyanto Raharjo ◽  
Monica Monica
Keyword(s):  
Body Weight ◽  
Total Cholesterol ◽  
Waste Cooking Oil ◽  
Control Group ◽  
Fruit Peel ◽  
Control Group Design ◽  
Cooking Oil ◽  
Used Cooking Oil ◽  
Post Test ◽  
Per Oral

Waste cooking oil causes increased level of total cholesterol and LDL and decreased level of HDL serum, which became coronary heart disease. The aim of this study was to evaluate the effect of mangosteen peels extract, which contain alpha-mangostin (xanthone) to decreased level of total cholesterol and LDL and increased level of HDL in rats exposed to waste cooking oil per oral. This study used true experimental designs with randomized post-test only control group design. This study used thirty rats were dividing into six treatment group, which control with Na-CMC 0,5%, control with extract of mangosteen pericarp at dosages of 800 mg/ kg body weight, exposed to waste cooking oil per oral, and exposed to waste cooking oil per oral with extract of mangosteen pericarp at dosages of 600, 800, and 1000 mg/kg body weight. All groups were given treatment for 12 weeks. After 12 weeks, rats were anesthetized with chloroform for collection of blood by cardiac puncture. Analysis of level of total cholesterol , LDL, and HDL serum with spectrophotometer. From Post Hoc test with Least Significant Differences it was concluded that extract of mangosteen peels can decreased level of total cholesterol and LDL and increased level of HDL serum.

scholarly journals Pemberian alpha lipoic acid per oral dan latihan fisik intensitas sedang menurunkan berat badan dan lemak abodminal lebih banyak daripada latihan fisik intensitas sedang saja pada tikus Wistar jantan dengan obesitas

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Aji B. Chandra ◽  
Alex Pangkahila ◽  
Wimpie Pangkahila
Keyword(s):  
Body Weight ◽  
Physical Exercise ◽  
Lipoic Acid ◽  
Abdominal Fat ◽  
Control Group ◽  
Alpha Lipoic Acid ◽  
Control Group Design ◽  
Post Test ◽  
Visceral Abdominal Fat ◽  
Per Oral

Abstract: This study was aimed to prove that oral administration of ALA and moderate physical exercise decrease body weight and abdominal fat more than moderate physical exercise only in obese male Wistar rats. This was a true experimental study with a post-test only control group design. Subjects were 30 Wistar rats (Rattus norvegicus), male, healthy, aged 4-5 months, and obese with minimum weight of 250 g, divided into 3 groups, as follows: 1) P0, the control group with no treatment; 2) P1, given moderate physical exercise (swimming for 20 minutes/day, for 4 weeks); 3) P2, given moderate physical exercise and ALA per oral 15 mg/day for 4 weeks. The results showed that the average body weight after 4 weeks of treatment of P0 group was 279.10±5.84 g; of P1 group was 257.90±10.31 g; and of P2 group was 213.90±8.92 g (P < 0.01). The mean weight of subcutaneous abdominal fat of P0 group was 0.96±0.45 g; of P1 group was 0.63±0.31 g; and of P2 group was 0.40±0.23 g (P < 0.01 ). Moreover, the mean weight of visceral abdominal fat of P0 group was 1.23±0.37 g; of P1 group was 0.83±0.24 g; and of P2 group was 0.39±0.25 g (P < 0,01). Conclusion: Oral administration of ALA and moderate physical exercise decrease body weight, subcutaneous abdominal fat, and visceral abdominal fat more than moderate physical exercise only in obese male Wistar rats.Keywords: alpha lipoic acid, obesity, body weight, abdominal fatAbstrak: Penelitian ini bertujuan untuk membuktikan bahwa pemberian ALA per oral yang dikombinasi dengan latihan fisik intensitas sedang menurunkan berat badan, lemak subkutan abdominal, dan lemak viseral abdominal lebih banyak daripada latihan fisik intensitas sedang saja pada tikus Wistar jantan dengan obesitas. Jenis penelitian ialah eksperimental murni dengan post-test only control group design. Subjek penelitian ialah 30 ekor tikus putih (Rattus norvegicus) jantan, galur Wistar, sehat, usia 4-5 bulan, dan obes dengan berat badan minimal 250 g yang terbagi menjadi tiga kelompok masing-masing berjumlah 10 ekor tikus, yaitu: P0 (kelompok kontrol), tidak mendapat perlakuan apapun; P1, kelompok perlakuan 1 yang diberikan latihan fisik intensitas sedang (renang durasi 20 menit/hari selama 4 minggu); dan P2, kelompok perlakuan 2 yang diberikan latihan fisik intensitas sedang serta ALA per oral dengan dosis 15mg/hari selama 4 minggu. Hasil penelitian menunjukkan bahwa rerata berat badan setelah 4 minggu perlakuan pada kelompok P0 ialah 279,10±5,84 gr; pada kelompok P1 257,90±10,31 gr; dan pada kelompok P2 213,90±8,92 gr (P < 0,01). Rerata berat lemak subkutan abdominal pada kelompok P0 ialah 0,96±0,45 gr; pada kelompok P1 0,63±0,31 gr; dan pada kelompok P2 0,40±0,23 gr (P < 0,01). Selain itu, rerata berat lemak viseral abdominal pada kelompok P0 ialah 1,23±0,37 gr; pada kelompok P1 0,83±0,24 gr; dan pada kelompok P2 0,39±0,25 gr (P < 0,01). Simpulan: Pemberian ALA per oral dengan latihan fisik intensitas sedang dapat menurunkan berat badan, lemak subkutan abdominal, dan lemak viseral abdominal lebih banyak daripada latihan fisik intensitas sedang saja pada tikus Wistar jantan dengan obesitasKata kunci: alpha lipoic acid, obesitas, berat badan, lemak abdominal

PENGARUH EKSTRAK SAMBILOTO (Andrographis paniculata [Burm f..] Ness) TERHADAP HITUNG EOSINOFIL MENCIT MODEL ALERGI INFLAMASI

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Arisanty Nursetia R
Keyword(s):  
Andrographis Paniculata ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Per Oral

Sambiloto mengandung flavonoid dan andrografolid yang mempunyai efek anti alergi. Penelitian ini bertujuan untuk mengetahui Pengaruh Ekstrak Sambiloto (Andrographis paniculata [Burm f.] Ness) terhadap hitung Eosinofil Serum Mencit Model Alergi Inflamasi. Jenis penelitian ini adalah eksperimental laboratorik, dengan post test only control group design. Hewan uji berupa 15 ekor mencit jantan. Mencit dibagi menjadi tiga kelompok, masing-masing terdiri dari 5 ekor. Kelompok I (kontrol) hanya diberi diet standar. Kelompok II diberi diet standar dan ekstrak sambiloto per oral dengan dosis 10mg/20 gram BB/hari. Kelompok III diberi diet standar dan antihistamin per-oral dengan dosis 0,4 mg/20 gram BB/hari. Sensitisasi mencit dengan ovalbumin (OVA). Hari ke-4 dan ke-20 dipapar OVA i.p. Dosis 0,15 cc/mencit. OVA inhalasi ke-37 secara intraorbital. Analisis data dengan uji Anova menggunakan program SPSS for Windows Release 11.5. Hasil penelitian menunjukkan rata-rata eosinofil mencit kelompok I = 0,94% kelompok II = 0,87% dan kelompok III = 1,2%. Dengan analisa statistik kelompok II dibandingkan kelompok I, kelompok III dibandingkan kelompok I dan kelompok II dibanding kelompok III menunjukkan perbedaan hitung eosinofil yang tidak bermakna (p>0,05). Ekstrak sambiloto dengan dosis 10 mg/20gram BB/hari dapat menurunkan hitung eosinofil serum pada mencit model alergi inflasi.

scholarly journals The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats

2017 ◽  
Vol 1 (1) ◽  
pp. 28
Author(s):  
Eviana Budiartanti Sutanto ◽  
Taufiq R Nasihun ◽  
Israhnanto Isradji ◽  
Luciana Budiati Sutanto
Keyword(s):  
Vitamin C ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Control Group ◽  
Control Group Design ◽  
Normal Sperm ◽  
Post Test ◽  
Male Wistar Rats ◽  
Post Hoc ◽  
Per Oral

Introduction: Cigarette smoke causes oxidative stress which results in reduced sperm concentration, motility and morphology, also increased levels of 8-OHdG as a marker of DNA damage. Vitamin C and E have potential role in repairing spermatozoa damages. The aim of this study was to determine the effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats.Methods: This study used a post test only control group design among 18 male Wistar rats subject, aged 8 week, 150-200 grams body weight (BW). The subject was randomly divided into 3 groups, K1: control, K2: cigarettes smoke exposed, K3: cigarettes smoke exposed and given a combination of 0.045 mg/gBW vitamin C and 0.036 IU/gBW vitamin E per oral. Analysis was done on day 21 using one-way ANOVA and post-hoc LSD for sperm concentration, motility and morphology; using Kruskal-Wallis and Mann-Whitney tests for cement 8- OHdG levels.Results: The lowest sperm concentration was found in   K2 (K2  32.59  million/mL,  K1 47.91 million/mL, K 339.43 million/mL); the lowest normal sperm motility was found in K2 (K 238.97%, K 164.57%, K3 51.43%); the lowest normal sperm morphology was found in K2 (K2 27.56%, K 138.36%, K 331.18%); and the highest cement 8- OHdG level was found in K2 (K2 20.18ng/mL, K1 3.43ng/mL, K3 5.28ng/mL).Conclusion: Combination of vitamin C and E can improve sperm concentration, motility and morphology and decrease cement 8-OHdG levels of smoke exposed rats.

scholarly journals Potential Development of Chicken Egg Shell in Recycling of Waste Cooking Oil Innovation through the Stirer Chamber Device

2021 ◽  
Vol 9 (E) ◽  
pp. 1256-1260
Author(s):  
Narwati Narwati ◽  
Hadi Suryono ◽  
Setiawan Setiawan
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Sampling Technique ◽  
Quality Standard ◽  
Waste Cooking Oil ◽  
Control Group ◽  
Control Group Design ◽  
Cooking Oil ◽  
Before And After ◽  
Shell Powder

BACKGROUND: Waste cooking oil (WCO) is overused for frying food can be a series of chemical changes, such as rancid, oxidation, and decomposition. Most of the secretion of toxic compounds are a product of oxidizing fatty acids, especially double-unsaturated fatty acids. The intervention of stirrer chamber unit and utilization of chicken egg’s shell as an absorbent is known to be able to improve the quality of WCO, includes reducing the number of peroxide and the free fatty acids (FFA). AIM: The purpose of this study was to analyze the effect of the powdered chicken egg’s shell on the number of peroxide and the WCO’s FFA through the stirrer chamber. METHODS: The research design used a simple experiment type post-test only Control Group Design. The subject was divided randomly into two groups, the treatment was given to one group as a control group and another group (other treatment) as an experimental group. The WCO sample called “Jelantah” for each treatment was 100 ml as much as 48 samples. The replication was carried out twice with 24 treatments of the mass of egg’s shell powder that was 0 g, 5 g, 10 g, and 15 g at a stirring time for 15 min, 30 min, and 45 min before and after heating 35°C using 150 rpm of stirring speed. The sampling technique used purposive sampling and the data were analyzed by Anova statistical test. RESULTS: Based on the result from this research showed that the original WCO contained the number of peroxide and FFA exceeded the quality standard of SNI 3741 (2013) that is 16.7 MekO2/kg and 0.69% in sequence. The average number of peroxide of control group before heating was 15.31 MekO2/kg and after heating was 17.4 MekO2/kg, while the FFA before heating was 0.61%, and after heating was 0.71%. The number of peroxide of the treatment group before heating was 12.83 MekO2/kg and after heating was 6.98 MekO2/kg, while the FFA content before heating was 0.46% and after heating was 0.25%. CONCLUSION: It was concluded that chicken egg’s shell powder could minimize the content of peroxide and WCO’s FFA through the stirrer chamber.

scholarly journals PENGARUH PEMBERIAN SAGU DIBANDING NASI TERHADAP BERAT BADAN TIKUS WISTAR

e-CliniC ◽  
2013 ◽  
Vol 3 (2) ◽  
Author(s):  
Eny A. Watumlawar ◽  
Sarah M. Warouw ◽  
Stefanus Gunawan
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Wilcoxon Test ◽  
Control Group ◽  
Increase Body Weight ◽  
Control Group Design ◽  
Tissue Mass ◽  
Group Design ◽  
Post Test ◽  
Male Wistar Rats

Abstract: Weight is one measure that gives description of the tissue mass including body fluids. Weight change is nfluenced by nutriment consumption. Sago contains high carbohydrates that can increase body weight. This study aimed to obtain the effect of sago compared to rice to body weight of wistar rats. This was a random laboratory experimental pre-post test with control group design. Subjects were male wistar rats, aged 5-6 months. The rats were divided in two groups: rice group as control and sago group. Sago was cooked in papeda form as much as 75 g of dried sago and 300 mL water. The rats were fed for 2 weeks. Data were analyzed by using the Wilcoxon test. The results showed that the sago group showed an increase of body weight significantly (p=0,001) meanwhile the rice group lose body weight significantly (p=0,001). Conclusion: Sago can increase body weight of wistar rats significantly.Keywords: body weight, sago, riceAbstrak: Berat badan merupakan salah satu ukuran yang memberikan gambaran massa jaringan termasuk cairan tubuh. Salah satu yang memengaruhi berat badan yaitu dengan mengonsumsi makanan bergizi. Sagu memiliki kandungan karbohidrat (pati) yang besar dan dapat meningkatkan berat badan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sagu terhadap berat badan dibanding nasi pada tikus wistar. Desain penelitian ini ialah eksperimental laboratorium acak pre-post test with control group design. Subjek penelitian ialah tikus wistar jantan berusia 5-6 bulan dibagi atas 2 kelompok: kelompok nasi (kontrol) dan kelompok sagu. Sagu dimasak dalam bentuk papeda sebanyak 75 g sagu kering dan 300 mL air. Tikus diperlihara selama 2 minggu. Berat badan diukur dengan menggunakan timbangan. Data dianalisis dengan uji Wilcoxon. Hasil penelitian menunjukkan bahwa pemberian sagu sebanyak 2 cc setiap hari selama seminggu mampu meningkatkan berat badan secara bermakna (p = 0,001) sedangkan pemberian nasi menurunkan berat badan (p=0,001). Simpulan: Pemberian sagu dapat meningkatkan berat badan tikus wistar secara bermakna.Kata kunci: berat badan, sagu, nasi

scholarly journals Efektivitas Kombinasi Ekstrak Kunyit (Curcuma Domestica Val) dan Madu Terhadap Ulkus Lambung Mencit BALB/c Akibat Pemberian Aspirin Secara Mikroskopis

2018 ◽  
Vol 7 (1) ◽  
pp. 26
Author(s):  
Sarianti Br Simbolon ◽  
Yusticia Katar ◽  
Selfi Renita Rusjdi
Keyword(s):  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Per Oral

Aspirin adalah golongan OAINS dengan efek samping yang paling sering adalah ulkus lambung. Kunyit (Curcuma Domestica val) dan madu memilki aktivitas dalam mencegah ulkus lambung. Tujuan penelitian ini adalah menentukan apakah kombinasi ekstrak kunyit dan madu mempunyai efektivitas yang lebih baik dalam mengurangiulkus lambung akibat pemberian aspirin dibandingkan ekstrak kunyit atau madu saja pada mencit BALB/c secara mikroskopis. Penelitian eksperimental post test only control group design telah dilakukan dari Januari 2016 sampai Januari 2017 menggunakan 30 ekor mencit yang semua diberikan aspirin 5.2 mg/gBB untuk menyebabkan ulkus pada lambung, kemudian dibagi menjadi kelompok kontrol (K) hanya diberi aspirin, perlakuan 1 (P1) diberikan larutan ekstrak kunyit dosis 10 mg/gBB, (P2) madu 0.04 ml/gBB, (P3) kunyit 10 mg/gBB + madu 0.04 ml/gBB, (P4) Kunyit 30 mg/gBB + madu 0.12 ml/gBB, diberikan selama 3 hari per oral. Hari ke-4 mencit di eutanasia menggunakan eter, kemudian diperiksa secara mikroskopis. Hasil pada kelompok P1, P2, P3 dan P4 dibandingkan dengan K dan begitu juga P4 dibandingkan dengan P3, P2, P1 menunjukkan hasil yang signifikan (p<0.05), sedangkan P3 dibandingkan dengan P2 dan P1 menunjukkan hasil tidak signifikan (p>0.05). Simpulan studi ini adalah kelompok P4 memilikiefektivitas lebih baik dalam mengurangi ulkus lambung mencit dibandingkan dengan P3, P2 dan P1.

Effect of herbal extract of anting-anting (Acalypha australis) on blood glucose level of Balb/C mice with induction of Streptozotocin

2011 ◽  
Vol 9 (1) ◽  
pp. 12-16
Author(s):  
RIZKY OCKTARINI ◽  
DIDING HERI PRASETYO ◽  
IPOP SJARIFAH
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
T Test ◽  
Herbal Extract ◽  
Control Group ◽  
Buffer Solution ◽  
Control Group Design ◽  
Post Test

Ocktarini R, Prasetyo DH, Sjarifah I. 2011. Effect of herbal extract of anting-anting (Acalypha australis) on blood glucose level of Balb/C mice with induction of Streptozotocin. Biofarmasi 9: 12-16. This study was conducted to determine the effect of herbal extract of anting-anting (Acalypha australis L.) on the decrease of blood glucose level of Balb/C mice induced with Streptozotocin. This research was an experimental laboratory with pre and post-test control group design, conducted in Laboratory of Biochemistry and Histology, Faculty of Medicine, Sebelas Maret University, Surakarta. The test animals used were 16 male mice, about 4-6 weeks of age with approximately 20-30 grams induced with Streptozotocin in 0.02 M citric buffer solution, and a dose of 65 mg/kg body weight. Furthermore, the mice were divided into two groups (Metformin in a dose of 1.3 mg/mice/day and herbal extract of anting-anting 1000 mg/kg BB/day) randomly, each group consisted of 8 mice. All mice were observed for GDS level after 14 days treatment period. The data obtained was processed by using the Independent-Samples T-test statistical test (unpaired t-test) with SPSS for Microsoft Windows Release 18.0. Mean of decreased GDS levels on Metformin group vs herbal extract of anting-anting (145.87 mg/dl vs 144.62 mg/dl, p=0.965). The treatment of herbal extract of anting-anting in a dose of 1000 mg/kg body weight/day could decrease blood glucose level of Balb/C mice induced with Streptozotocin that equal with Metformin.

scholarly journals Pengaruh Ekstrak Propolis (Metode CMCE) Terhadap Kadar Malondialdehid (MDA) Dan Degenerasi Tubulus Renalis

2021 ◽  
Vol 2 (1) ◽  
pp. 1-7
Author(s):  
Alamsyah Alamsyah ◽  
Shofa Chaasani ◽  
Joko Wahyu Widodo ◽  
Taufiqurrachman Nasihu ◽  
Chodidjah Chodidjah ◽  
...  
Keyword(s):  
Blood Urea Nitrogen ◽  
Control Group ◽  
Control Group Design ◽  
Urea Nitrogen ◽  
Group Design ◽  
Post Test ◽  
Per Oral ◽  
One Way Anova

Gangguan ginjal akut (GnGA) merupakan suatu keadaan dimana proses laju filtrasi glomerulus ginjal menurun secara cepat yang menyebabkan retensi nitrogen terutama kreatinin dan blood urea nitrogen (BUN). Kondisi ini dapat dinetralkan dengan mengkonsumsi antioksidan dari luar tubuh seperti Propolis (metode CMCE). Untuk mengetahui pengaruh ekstrak propolis (metode CMCE) terhadap kadar MDA dan degenerasi tubulus renalis. Penelitian ekperimental dengan pendekatan post test only control group design. Subyek penelitian berjumlah 25 ekor tikus jantan galur wistar yang dibagi secara acak menjadi 5 kelompok. Kelompok K1 tanpa diinduksi gentamisin. Kelompok K2 diinduksi gentamisin dan tanpa diberi ekstrak propolis metode CMCE. Kelompok P1, P2 dan P3 diinduksi gentamisin dan ekstrak propolis metode CMCE masing-masing dengan dosis 200, 400, 800 mg/k, BB per hari per oral selama 7 hari. pemeriksaan kadar Malondialdehid (MDA) di IBL FK UNISSULA dan pemeriksaan degenerasi tubulus renalis di RSI Sultan Agung Semarang pada Maret - Juli 2020. Uji One Way Anova menunjukkan perbedaan bermakna pada kadar MDA dan degenerasi tubulus renalis  menunjukkan perbedaan bermakna (p<0,05). Pemberian ekstrak propolis metode CMCE menunjukan berpengaruh secara signifikan terhadap kadar MDA dan skor total degenerasi tubulus renalis yang diinduksi gentamisin.

scholarly journals The effect of meniran herbs extract (Phyllantus niruri) to triglycerides blood level in wistar rats

2012 ◽  
Vol 10 (1) ◽  
pp. 23-27
Author(s):  
JUDO YUSTANTO KAHONO ◽  
KISRINI KISRINI ◽  
YUL MARIYAH
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Total Period ◽  
Group I ◽  
Significant Difference ◽  
Post Test ◽  
Group Ii

Kahono JY, Kisrini, Mariyah Y. 2012. The effect of meniran herbs (Phyllantus niruri) extract to blood triglyceride level in rats. Biofarmasi 10: 23-27. The aim of this experiment was to determine the effect of meniran herbs (Phyllantus niruri L.) extract to blood triglycerides level in Wistar rats (Rattus norvegicus). This experiment was a laboratory experiment with pre and post-test with a control group design. The samples were 30 male Wistar rats with ±200 gram of body weight and ±2 months old. The samples were divided into five groups by using a purposive random sampling technique and each group consisted of six rats. Group I as a negative control, group II as a positive control, and groups III, IV and V as meniran herbs extract groups with first dose, second dose, and third dose. All rats were simultaneously given with hyperlipemic feed. Group II was simultaneously administered with Cholvastin 0.5 mg/200 grams body weight/day. Groups III, IV and V were simultaneously treated with meniran herbs extract at dose of 25 mg, 50 mg and 100 mg/200 grams body weight/day. The total period of this experiment was 21 days. At the day 1st and 21st, blood was collected from rat orbital venous plexus. Serum was analyzed for their pre and post-test blood triglycerides. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by a post-hoc test. The value of p=0.002 (p<0.05) showed there was a significant difference between pre-test and post-test in delta means of blood triglycerides level among groups. Meniran herbs extract had a significant triglycerides lowering effect against blood triglycerides level of Wistar rats. The most potential of meniran herbs extract dose was 100 mg/200 gram body weight/day.

scholarly journals The Effectiveness Of Waste Cooking Oil Recycling Project-Based Learning To Improve Students’ High Order Thingking Skills

2019 ◽  
Vol 3 (1) ◽  
pp. 29-34
Author(s):  
Yeni Sarif ◽  
Noor Fadiawati ◽  
M. Mahfudz Fauzi Syamsuri
Keyword(s):  
Thinking Skills ◽  
Waste Cooking Oil ◽  
Project Based Learning ◽  
High Order ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Cooking Oil ◽  
Analysis Technique ◽  
High Order Thinking

This study aimed to describe the effectiveness in project-based learning of waste cooking oil recycling to improving students’ high order thinking skills. The research design used was the The Matching-Only Pretest-Posttest Control Group Design. The population in this research was all of students in grade XI MIA one of Senior High School in Bandarlampung. The sample of the research was the students of class XI MIA 4 and XI MIA 6. The data analysis technique used was non parametric statistic test using Mann Whitney U to postestt value. The results of this study it can be seen from postest value. Postest value in the experiment class that is greater than the postest value in the control class as well as the <g> on the medium categorized in the experimental class and in the low control class indicate that project-based learning of waste cooking oil recycling can improve students' high order thinking skills. Keywords: high order thinking skills, waste cooking oil, project-based learning                                                               

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