scholarly journals Formation features of humoral immunity in newborn calves in case of including haylage with bio-preservative into the diet of cows

2021 ◽  
Vol 273 ◽  
pp. 02008
Author(s):  
Anna Karamaeva ◽  
Larisa Bakaeva ◽  
Natalia Soboleva ◽  
Sergey Karamaev

The peculiarities of the formation of colostrum in the udder of cows and the immune status in the body of calves in the first hours and days after birth, when included in the diet of cows, haylage from eastern goat’s rue prepared with the use of bio-preservatives “Silostan” (group II) and “GreenGrass 3 × 3”(Group III). The study of colostrum of the first milk yield showed that feeding cows with silage with the bio-preservative “Silostan” contributed to an increase in the content of immunoglobulins by 4.0% (Р<0,001), with bio-preservative “GreenGras 3 × 3” - by 5.3% (Р<0,001). After drinking the first portion of colostrum, immunoglobulins appear in the blood serum of calves after 2 hours. After 6 hours, the content of immunoglobulins in the blood serum of calves of group II was 21.9% higher than in group I (haylage without preservative) (P < 0.05), group III - by 27.1% (P <0.001). The proportion of calves with an immunoglobulin content 6 hours after the first feeding with colostrum not more than 6.0 mg / ml, decreased in group II by 12.0%, in group III - by 20.0%.

2018 ◽  
Vol 85 (11) ◽  
pp. 26-28
Author(s):  
A. A. Ibrahimova ◽  
N. Yu. Bayramov ◽  
A. M. Rustam

Objective. To study up a connection between indices of the functional hepatic tests and  the body mass index (BMI) in patients after the operation. Маterials and methods. In 59 оperated patients a BMI was calculated, as well as biochemical hepatic tests: activity of alaninaminotransferase (AlAT) in the blood serum, aspartataminotransferase (АsAТ), γ-glutamintransferase (γ-GТ) and a general bilirubin content. Basing on the BMI indices, all the patients were divided into three groups. Into Group I 30 patients were included with BMI 18.5 - 24.9 kg/m2, into Group II - 20 patients with excessive body mass, the BMI values 25.0 - 29.9 9 kg/m2, and into Group III - 9 patients with obesity, the BMI values more than 30 9 kg/m2. The blood serum activity of AlAT, AsAT, γ-GТ, alkaline phosphatase, indices of bilirubin, international normalized ratio and C-reactive protein were determined in accordnce to standard mrthods. In all the patients the data of hepatic function testing were analyzed before the operation and in 1, 3, 5 days after the operation. Results. In Groups I and II the values of АlAТ, АsAТ and γ-GТ were in borders of normal range. The changes were observed on the first and third postoperative day in Group III. Average activity (±SD) of the blood serum АlaТ and АsAТ in Group I have constituted (25.7 ± 9.8) and (24.9 ± 7.8) U/L (p < 0.01), in Group II - (32.5 ± 9.1) and (25.2 ± 7.1) U/L (p < 0.01), in Group III - (46.2 ± 13.5) and (31.9 ± 10.6) U/L (p < 0.01). Conclusion. In high BMI in patients, who had hepatic fatty degeneration, a hepatic enzymes activity was raised significantly, comparing with patients who had not hepatic fatty degeneration. Raised activity of hepatic enzymes in the blood serum was connected with high BMI. That’s why a high rate of hepatic fatty degeneration is observed in patients with raised BMI.


2013 ◽  
Vol 16 (4) ◽  
pp. 655-662 ◽  
Author(s):  
R. Bodarski ◽  
S. Kinal ◽  
J. Preś ◽  
M. Słupczyńska ◽  
J. Twardoń

Abstract The aim of the presented study was the estimation of optimal Ca and P levels applied before calving together with anionic salt addition, as an element of hypocalcaemia and hypophosphataemia prevention. The experiment was carried out during the dry period on 48 cows with similar milk yield in the previous lactation. Cows were divided into four groups. In group I (control) the amount of minerals was in accordance to NRC standards. In experimental groups (groups II-IV), two weeks before calving, cows received 140 g/day/head of hydrated magnesium sulphate to achieve dietary cation- anion difference at the level of about 50 mEq/kg DM. In groups II and III cows received calcium carbonate (100 g/day) 10 days a.p. (antepartum) (group II), or 5 days a.p. (group III), while cows in IV group received dicalcium phosphate (100 g/day) for 5 days a.p. Application of MgSO4 × 7H2O significantly affected the urine pH of cows from group III and IV 4-5 d. before calving - 6.45 and 6.81, respectively. The acidification of urine was observed after calving in group IV (7.13). In cows from group II (100 CaCO3 10 days a.p.) urine pH decline was not found (7.97-7.75). In that group the incidences of hypophosphatemia were noted (blood serum inorganic P level 1.41-1.46 mmol/l). Addition of magnesium sulphate prevented hypocalcaemia occurrence - 4-5 d. before calving the concentration of ionized Ca in blood serum was 1.11, 1.13 and 1.16 mmol/l (respectively for group II, III and IV). Reproductive functions were significantly improved after the application of CaCO3 and CaHPO4 for 5 days a.p. in comparison with control and group II - progesterone concentration in the blood serum on the 45th day of lactation was 1.396 - 1.409 vs 0.799 - 0.401. The correlation between progesterone and inorganic P level in serum was almost significant. Based on the obtained results a treatment optimal in prevention of hypocalcaemia and hypophosphataemia is the application of 50 g CaCO3 and 50 g of CaHPO4 for the last 5 days of the dry period together with MgSO4 × 7H2O given for 14 days a.p.


1969 ◽  
Vol 11 (4) ◽  
pp. 569-572
Author(s):  
M. A. Sharafeldin ◽  
I. A. Ramadan

SUMMARYAt birth, the Barki lambs used in this experiment were randomly allocated to three groups which numbered 133, 126 and 154 lambs of both sexes weaned at the ages of 10 weeks (Group I), 12 weeks (Group II) and 16 weeks (Group III) respectively.The most pronounced differences between the three groups of lambs in their body weights took place at the age from 4 to 6 months. Group II lambs performed as well as or even slightly better than the other two groups, which leads to the conclusion that as far as the body weight of lambs is concerned there is no need to extend their suckling period more than 12 weeks.The three groups of lambs varied little in their mortality rates from 2·5 to 12 months of age.


2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Samuel Tung-Hsing Chiang ◽  
Shang-Min Yeh ◽  
Yi-Chen Chen ◽  
Shiun-Long Lin ◽  
Jung-Kai Tseng

The purpose of this study was to investigate the protective role of orally administered taurine against diabetic retinal changes via electroretinogram (ERG) and retinal histology on rabbits. Rabbits were randomly assigned into groups: Group I (vehicle administration only); Group II (diabetes: induced by 100 mg/kg alloxan injection); Group III (diabetes and fed with 200 mg/kg taurine); and Group IV (diabetes and fed with 400 mg/kg taurine). The body weight and blood glucose levels of the rabbits were monitored weekly. The ERG was measured on weeks 5 and 15. Retinal histology was analyzed in the end of the experiment. Results revealed that a taurine supplement significantly ameliorates the alloxan-induced hyperglycemia and protects the retina from electrophysiological changes. Group II showed a significant(P<0.05)change in the mean scotopic b-wave amplitude when compared to that of Group I, whereas the diabetic rabbits treated with taurine (Group III and IV) were analogous to Group I. Histologically, the amount of Bipolar and Müller cells showed no difference(P>0.05)between all groups and when compared with those of Group I. Our study provides solid evidences that taurine possesses an antidiabetic activity, reduced loss of body weight, and less electrophysiological changes of the diabetic retina.


2017 ◽  
Vol 73 (12) ◽  
pp. 756-763
Author(s):  
Anna Szuba-Trznadel ◽  
Tomasz Hikawczuk ◽  
Adam Ciura ◽  
Bogusław Fuchs

The study was conducted on sows (hybrids of wbp × pbz breeds) and their offspring (until day 75 of life) kept on a farm. The aim of the experiment was to compare the effects of different sources of selenium (Se) on the production performance of the animals, Se content in their blood, the level of Se in sow’s colostrum, as well as Gpx, haptoglobin and immunoglobulin levels in the serum of sows and their offspring. Experimental feed mixtures for pregnant sows (LP), lactating sows (LK) and piglets (prestarter and starter) in each treatment had an identical basic composition, differing only in the type of selenium forms. Group I received a mineral form of Se in an amount of 0.2 mg/kg; group II received a mixture of a Se amino acid chelate and the mineral form of Se (0.1 mg/kg of each); group III received a Se amino acid chelate (0.2 mg/kg), and group IV received Se-enriched yeasts (0.2 mg/kg). Beneficial effects of the organic forms of Se were evident already in the lactation period. Sows, especially those from group II receiving 0.2 mg/kg of organic Se, had a higher feed intake, which was related to a higher milk production during lactation. As a result, on the weaning day, piglets from this group were significantly heavier than the other piglets. After weaning, as well, the piglets in this group were significantly heavier. These results were confirmed by parameters of blood serum and whey colostrum. Selenium as a chelate was more available than the mineral and enriched yeast forms. For this reason, the animals receiving the chelate were healthier (fewer inflammations were noted). The animals in this group also showed a better feed conversion compared with the others. The Gpx level in sows’ serum varied depending on the treatment. The highest level of this parameter was determined in sows from group III (receiving 0.2 mg/kg of organic Se), and it differed significantly from its value in the control group. The results showed that the Gpx level was related to the Se concentration in blood serum, which was also confirmed by a higher production of selenocysteine (a part of Gpx). Cells of the animals from this group were better protected against free radicals. Administration of 0.1 mg/kg of organic Se positively affects the performance of animals, but the recommended level in feed is 0.2 mg/kg of a selenium-containing amino acid....


2013 ◽  
Vol 1 (3) ◽  
Author(s):  
Eka Sari Tappi ◽  
Poppy Lintong ◽  
Lily Loho

Abstract: Liver is the largest organ in the abdominal cavity. As the center of metabolism in the body, liver is potentially damaged by exposure of toxic substances, inter alia carbon tetrachloride (CCl4). Metabolism of carbon tetrachloride (CCl4) produces CCl3 free radicals that can damage the liver. In Indonesia, there are a lot of natural ingredients that have antioxidant properties, such as tomato. Lycopene in tomatoes contains antioxidant compounds that can prevent damages due to free radical. This study aimed to obtain liver histopathological changes of wistar rats fed with tomato juice after being induced of carbon tetrachloride (CCl4). This was an experimental study, using 10 wistar rats which were divided into 4 groups. Group I was the negative control; group II was induced with CCl4 0,05 cc/day and was terminated on day 6; group III was induced with CCl4 0,05 cc/day and was given tomato juice 3 ml/day, and terminated on day 13; group IV was induced by CCl4 0,05 cc/day, given regular pellets, and terminated on day 13. The results showed that group II had histopathological changes of the liver indicating fatty liver, meanwhile group III showed regeneration of nearly all liver cells. Conclusion: Administration of tomato juice after the induction of 3 ml carbon tetrachloride (CCl4) for 7 day showed regeneration of almost all liver cells. Keywords: histopathological changes of the liver, carbon tetrachloride, tomato juice.   Abstrak: Hati merupakan organ terbesar dalam rongga abdomen, dan pusat metabolisme tubuh dengan fungsi yang sangat kompleks dan sangat berpotensi mengalami kerusakan akibat terpapar oleh bahan-bahan toksik, salah satunya yaitu karbon tertraklorida (CCL4). Metabolisme CCl4 menghasilkan radikal bebas CCl3 yang dapat merusak hati. Di Indonesia terdapat  banyak sekali bahan-bahan alami yang mempunyai kandungan antioksidan, salah satunya yaitu tomat. Tomat mengandung senyawa likopen sebagai antioksidan yang dapat mencegah kerusakan jaringan akibat radikal bebas. Penelitian ini bertujuan untuk mendapatkan gambaran histopatologi hati tikus wistar yang diberi jus tomat pasca induksi karbon tetraklorida (CCl4). Metode penelitian ialah eksperimental. Sampel sebanyak 10 ekor tikus wistar yang dibagi dalam 4 kelompok. Kelompok I sebagai kontrol negatif; kelompok II diinduksi CCl4 0,05cc/perhari dan diterminasi hari ke-6; kelompok III diinduksi CCl4 0,05 cc/hari kemudian diberikan jus tomat 3ml/hari;  dan kelompok IV diinduksi CCl4 0,05 cc/hari kemudian diberikan pelet biasa dan diterminasi hari ke-13. Hasil penelitian menunjukkan pada pemberian CCl4 pada tikus wistar selama 5 hari terdapat gambaran morfologik perlemakan sel hati. Pemberian jus tomat dosis 3 ml pasca induksi karbon tetraklorida (CCl4) menunjukkan terjadinya regenerasi pada hampir seluruh sel-sel hati. Simpulan: Pemberian jus tomat dosis 3 ml pasca induksi karbon tetraklorida (CCl4) selama 7 hari menunjukkan regenerasi pada hampir seluruh sel-sel hati. Kata kunci: gambaran histopatologi hati, karbon tetraklorida, jus tomat.


Author(s):  
O.V. Vlasova ◽  

The aim is to determine the indicators of humoral immunity of patients with neonatal sepsis under different conditions of environmental pollution of their parents' places of residence. Materials and methods. 260 patients with neonatal sepsis born to parents who lived in different environmental conditions were examined. An environmental risk factor (CER) was proposed as a group-forming feature of a comprehensive assessment of the long+term load of anthropogenic air, water and soil pollution in the regions of the region on the body of parents of newborns, taking into account the environmental situation in regional centers. Results. The level of IgA in the serum of patients with neonatal sepsis in group I was 0.82±0.11 g/l against 1.54±0.23 g/l (p<0.05) in group II, the level of IgG in children I groups — 4.27±0.45 g/l against 6.67±0.77 g/l (p<0.05) of group II. The decrease in IgM content had no significant differences, but there was a tendency to reduce its level in children of group I. Conclusions. The constant residence of parents in areas with a high risk of adverse effects of these environmental factors on their body (ERF<2.0) contributed to a decrease in the level of immunoglobulins A, M, G in the serum of patients with neonatal sepsis, compared with newborns whose parents lived in areas with a low risk of adverse effects (ERF<2.0), and were more likely to have severe neonatal sepsis. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of these Institutes. The informed consent of the patient was obtained for conducting the studies. No conflict of interest was declared by the author. Key words: neonatal sepsis, pollution, immunoglobulins.


Author(s):  
S.A. OVODKOV ◽  
Ah.S. DELYAN

Изучено влияние способа содержания и сезона отела на лактационную деятельность голштинизированных коров-первотелок черно-пестрой породы. Исследования проведены в условиях сельскохозяйственного предприятия Тверской области на 4 группах животных: I группа — привязное содержание, летние отелы; II — привязное содержание, зимние отелы; III — беспривязное содержание, летние отелы; IV — беспривязное содержание, зимние отелы. Максимальные удои у коров I, III и IV групп были получены на 3-м мес лактации, II — на 2-м. Удой в I, II, III и IV группах составил, соответственно, 7714, 8717, 7682 и 7930 кг молока за 305 дней лактации. Удой коров за первые 3 мес в % к удою за 305 дней лактаций: I группа — 33,7%, II — 35,8, III — 34,4, IV — 35,9%. Максимальный суточный удой в I группе равнялся 30,8 кг, во II — 36,5, в III —29,7 и в IV — 35,3. Среднесуточный удой за 305 дней лактации в I группе составил 21,2 кг, во II — 23,6, в III группе — 19,4 и в IV группе — 21,7. Коэффициент постоянства лактации в I II III и IV группах равнялся 93,2, 94,2, 89,2 и 98%, соответственно. Независимо от способа содержания более высокие показатели молочной продуктивности и устойчивости лактационной деятельности имели коровы, отелившиеся в зимний период.The influence of the method of keeping and calving season on the lactation activity of Holstein cows-heifers of black-and-white breed is studied. Studies were conducted in the agricultural enterprise of the Tver region on 4 groups of animals: I group — tethered content, summer calving; 2 — tethered content, winter calving; III — loose content, summer calving; 4 — loose content, winter calving. Maximum milk yield in cows I and III and IV groups were obtained on the 3rd month of lactation, the second — at the 2nd. Milk Yield in I, II and III and in IV groups made, respectively, 7714, 8717, 7682 and 7930 kg of milk for 305 days of lactation. Milk yield of cows for the first 3% to milk yield for 305 days of lactation: group I-33,7%, II — 35,8, III-34,4, IV-35,9%. The maximum daily yield in group I was 30.8 kg, in group II — 36.5, in group III — 29.7 and in group IV — 35.3. The average daily yield for 305 days of lactation in group I was 21.2 kg, in group II — 23.6, in group III — 19.4 and in group IV — 21.7. The coefficient of persistence of lactation in I, II, III and IV groups was equal to 93.2 per cent, 94,2, to 89.2 and 98%, respectively. Regardless of the method of maintenance, cows calving in winter had higher indicators of milk productivity and stability of lactation activity.


Author(s):  
L.N. Sayfutdinova ◽  
◽  
M.A. Derkho ◽  
◽  

The variability of corticosterone and cortisol concentrations and their ratio in laying hens during the development of a stress reaction, as well as the correlation of hormone levels with the variability of protein parameters in the blood, are evaluated. It was found that when the technological factor (the density of birds in the cage) changes by 1.5 times, the amount of glucocorticoids in the blood of birds during the stress reaction increases, both due to corticosterone and cortisol. Therefore, the balance between the hormones were not significantly changed and is in the range of 4.23-5,43conv. Units. When the technological factor is increased by 2 times, the content of corticosterone in the blood of birds mainly increases, determining an increase in the value of the corticosterone/cortisol ratio by 16.56-28.71%. The biological effects of glucocorticoids in the body of birds are realized by corticosterone, the level of which in a moderate and strong degree, including statistically significant, both under normal and stress conditions, is associated with the activity of transamination enzymes: Corticosterone-AlAT (r(group I) = 0,69±0,32 – 0,88±0,21; r(group II) =0.63±0.24 – 0.91±0.13; r(group III) = 0,59±0,22 – 0,76±0,16) and Corticosterone-AsAT (r(I group) = 0,57±0,36 – 0,83±0,25; r(group II) =0,68±0,23 – 0,72±0,22; r(group III) = 0,67±0,19 – 0,89±0,12), and also with the concentration of albumins during the development of a stress reaction (Corticosterone-Alb: r(group II) = -0,69±0,22 – -0,81±0,19; r(group III) = -0,58±0,21 – -0,81±0,16).


2019 ◽  
Vol 87 (4) ◽  
pp. 179-187
Author(s):  
Wioletta Waksmańska ◽  
Halina Woś ◽  
Rafał Bobiński

Aim. The aim of the research was to analyze the incidence of abnormal body weight values and to assess eating habits and physical activity of nurses.Material and Methods. The studies involved all first‑year nursing students of the second degree daily studies, working as a nurse. The women’s eating habits were analyzed based the diet. The questionnaire allowed the researchers to determine daily consumption of each particular dietary component (proteins, carbohydrates, fats, fatty acids, vitamins) as well as the women’s calorie consumption.Results. The analysis showed that the group of underweight participants was comprised of 5 students (group I), the group of participants with normal body weight — of 43 students (group II), and the group of participants with excessive body weight — of 10 students (group III). Group II students spent the longest time on (moderate and intense) physical activity (on average 378 minutes/week), whereas group III students — the shortest (on average 203 minutes/week). While analyzing the average protein intake, it was found that all groups exceeded its daily requirement. The intake of sodium and cholesterol was exceeded more than twice of the recommended amount. A very low intake of vitamin D — covering from 40 to 48% of the daily requirement — was observed in all groups.Conclusions. Despite the fact that the nurses’ diet includes all nutrients necessary for the body, it is not properly balanced which obliges to raising awareness of types of consumed food.


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