scholarly journals Total Phenol Content and Activities of Antioxidant Extracts Methanol Limes (Citrus Aurentifolia) By Uv-Vis Spectrophotometry

2021 ◽  
Vol 328 ◽  
pp. 01008
Author(s):  
Khadijah ◽  
Nunuk Hariani Soekamto ◽  
Siti Maisuri Tadjuddin Chalid ◽  
Nur Fatin Rafidah
Keyword(s):  
Antioxidant Activity ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Fruit Extract ◽  
A Value ◽  
Ic50 Value ◽  
Dpph Method ◽  
The Relationship ◽  
Phenol Extract

Total phenol levels and antioxidant activity in whole limes from Palopo, Luwu Regency, as well as the relationship between total phenol content and antioxidant activity, were determined. Maceration of dried whole lime fruit yielded the methanol extract (Citrus aurentifolia). Phytochemical screening was used to determine the presence of alkaloids, phenolic compounds, and flavonoids in the extract’s secondary metabolites. The total phenol extract was determined using the Folin-Ciocalteu method, yielding a value of 8.37 mgGAE/g. Antioxidant activity was determined using the DPPH method, and the IC50 value was 1793.06 g/mL. Total phenol content of whole fruit extract lime and IC50 show a linear relationship y = -168,11x + 3211 with a determination R2 = of 0.9780.

PERBEDAAN AKTIVITAS ANTIOKSIDAN MINUMAN SEGAR DAN MINUMAN INSTAN DARI JAHE (Zingiber officinale var rubrum), PALA (Myristica fragrans Houtt) DAN SERAI (Cymbopogon nardus L)

2021 ◽  
Vol 11 (2) ◽  
Author(s):  
Citra C. Dusun ◽  
Jan Rudolf Assa ◽  
Mercy I. R. Taroreh
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Zingiber Officinale ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Total Phenols ◽  
Phenol Content ◽  
A Value ◽  
Ic50 Value ◽  
Myristica Fragrans Houtt

AbstractThis study aims to obtain fresh and instant drink formulas from ginger, nutmeg and lemongrass that are accepted by the panelists and have the best antioxidant activity. This study used the RAL method with 6 treatments 3 replications, and fresh drink formulations of FA1: ginger 300mL, nutmeg 100mL, lemongrass 100mL, FA2: ginger 300mL, nutmeg 50mL, lemongrass 150mL, FA3: ginger 300mL, nutmeg 25mL, lemongrass 175 mL and instant drinks FA4: ginger 300mL, nutmeg 100mL, lemongrass 100mL, FA5: ginger 300mL, 50mL of nutmeg, 150mL of lemongrass, FA6: ginger 300mL, nutmeg 25mL, lemongrass 175 mL, then analyzed for total phenol content, antioxidant activity as an antidote to free radicals, then using analysis of variance (ANOVA). The results showed that the highest total phenol content was in the FA3 treatment of 10.81 mgGAE / g, and the lowest was in the FA4 treatment of 0.22 mgGAE / g. The results of the analysis of antioxidant activity as an antidote to DPPH free radicals at a concentration of 100ppm showed that the highest percentage of inhibition was obtained in the FA3 treatment of 52.18% and the lowest was in the FA4 treatment, namely 42.59%. Whereas for the IC50 value, the highest value was found in the FA3 treatment with a concentration of 56.8 ppm, and the lowest was in the FA4 treatment with a concentration of 116.05 ppm. The best treatment based on the sensory test of instant drinks, the most preferred by the panelists was in the FA5 treatment with a value of 4.35, and the lowest was in the FA6 treatment with a value of 4. The results of this study can be concluded that the fresh drinks that have the best total phenols are found in the FA3 treatment, where as for instant drinks that have the best total phenols in the FA5 treatment, and for fresh drinks and instant drinks that have the best antioxidant activity are in the FA3 treatment, for pelyan persistic preferences testing FA5.Keywords: Ginger, Nutmeg, Lemongrass, Total Phenol, Antioxidant.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN TOTAL KANDUNGAN FENOLIK EKSTRAK ETANOL DAUN NANAMUHA (Bridelia monoica Merr)

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 178
Author(s):  
Julia Elsa Lakoro ◽  
Max R. J. Runtuwene ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Total Phenol ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phenol Compounds ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ethanol Extracts

ABSTRACTNanamuha (Bridelia monoica Merr.) is a plant that was used as a cancer and tumor drug by the Sangihe Islands community. This plant contains phenol compounds, flavonoids, alkaloids and tannins. The purpose of this study was to determine the potential of nanamuha leaves that grow in the Sangihe Island to have antioxidant activity and determine the total phenolic ethanol extracts of nanamuha leaf. The extraction method used is maceration with ethanol solvent p.a. The extracts obtained was determined total phenolic and antioxidant activity test using DPPH method. Based on the results obtained, the ethanol extracts of nanamuha leaves has antioxidant activity with an IC50 value of 75,03 µg / mL and the result showed that the total phenol content the ethanol extracts of nanamuha leaves of  53,34 mg / L.  Keywords : Nanamuha leaf, total phenol, antioxidant activity, DPPH  ABSTRAKNanamuha (Bridelia monoica Merr.) merupakan tanaman yang dimanfaatkan sebagai obat kanker dan tumor oleh masyarakat Kepulauan Sangihe. Tanaman ini memiliki kandungan senyawa fenol, flavonoid, alkaloid dan tanin. Tujuan dari penelitian ini adalah unutuk mengetahui potensi dari daun nanamuha yang tumbuh di Kepulaun Sangihe ini memiliki aktivitas antioksidan dan menentukan total fenolik dari ekstrak etanol daun nanamuha. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol p.a. Ekstrak yang diperoleh ditentukan total fenolik dan uji aktivitas antioksidan dengan metode DPPH. Berdasarkan hasil yang didapatkan ekstrak etanol daun nanamuha memiliki aktivitas antioksidan dengan nilai IC50 75,03 µg/mL dan hasil penelitian menunjukan total kandungan fenolik ekstrak etanol daun nanamuha sebesar 53,34 mg/L. Kata kunci : Daun Nanamuha, Total fenol, Aktivitas antioksidan, DPPH

scholarly journals Antioxidant and Anti-Aging Activity of Green Mustard Ethanol Extract Gel

2021 ◽  
pp. 304-311
Author(s):  
Silvia Handayani ◽  
Edy Fachrial ◽  
Adek Amansyah ◽  
I. Nyoman Encrich Lister
Keyword(s):  
Antioxidant Activity ◽  
Oil Content ◽  
Ethanol Extract ◽  
The Body ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Ic50 Value ◽  
Dpph Method ◽  
Content Recovery ◽  
Better Than

The skin is the organ of the body that is frequently exposed to direct UV rays from the sun, which causes the formation of ROS (reactive oxygen species), leading to cell death and tissue damage. This health problem can be overcome by using antioxidants to stabilize the free radicals. Mustard as an organic substance which contains polysaccharide compounds, vitamin C, carotene, quercetin, routine compounds, kaempferol and β-cytostreol, all of these are beneficial to skin health and beauty. Therefore, this study aims to determine the antioxidant activity of the total flavonoids and phenol content of mustard ethanol extract using the DPPH method. Furthermore, the extract at concentrations of 2, 4, and 6% was also tested for its anti-aging activity using the following parameters: moisture and oil content, texture, collagen, wrinkles, pigment, sensitivity, and pores. The results showed that the antioxidant activity of the green mustard had an IC50 value of 170.7839 µg/ml, a total phenol content of 14.471 mg GAE/g extract and flavonoid content of 12.753 mg QE/g extract. The effectiveness of the aging activity of the extract was tested using formulation 6%, which is better than 2%, and 4%. Thus, the percentage of water content recovery, oil content, texture, collagen, wrinkles, stains, sensitivity and pores respectively was 36.41, 39.33, 64.07, 17.65, 65.28, 50.14, 49.10 and 35.09%.  Consequently, it was concluded that the extract has the potential of being developed into a herbal beauty product.

scholarly journals Antioxidant activity and total phenolic content in Caulerpa filiformis (Chlorophyta) from Sechura Bay and Paracas Bay, Peru

2020 ◽  
Vol 27 (1) ◽  
pp. 061-066 ◽  
Author(s):  
Joyce Mamani ◽  
Jorge Chávez ◽  
Eder Apumayta ◽  
Patricia Gil-Kodaka
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Methanolic Extract ◽  
Total Phenol ◽  
North Coast ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
The North

In Peru, Caulerpa filiformis is a marine algae listed as an invasive species. For years, its distribution has been considered to be in the north coast (Isla Lobos de Afuera and Piura) until a recent report of its distribution in the central coast (Ancash, Lima, and Ica). The present investigation aims to determine the main groups of secondary metabolites, total phenol content, and antioxidant activity of the methanolic extract of C. filiformis from Sechura Bay (Piura) and Paracas Bay (Ica). The main chemical groups were determined through phytochemical screening, the content of phenols by the Folin–Ciocalteu method, and antioxidant activity by the ABTS method (2,2-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical screening of the methanolic extract of C. filiformis from Sechura Bay and Paracas Bay revealed the presence of carbohydrates, polyphenols, tannins, flavonoids, lipids, alkaloids, steroids, and triterpenes for both extracts. The total phenol content of the extract of C. filiformis from Sechura Bay (39.31 ± 0.39 mg of AGE/g extract) was significantly higher (p < 0.05) than that from Paracas Bay (18.78 ± 0.31 mg of AGE/g extract). In the ABTS and DPPH assays, the antioxidant capacity of the Sechura C. filiformis extract (IC50 = 3.49 ± 0.01 and 2.18 ± 0.02 mg/mL) was significantly higher (p < 0.05) than that of the Paracas C. filiformis extract (IC50 = 6.41 ± 0.02 and 2.42 ± 0.04 mg /mL). These findings suggest that the methanolic extract of C. filiformis is a source of secondary metabolites with an antioxidant potential.

scholarly journals Chestnut Drying Is Critical in Determining Aspergillus flavus Growth and Aflatoxin Contamination

Toxins ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 530 ◽  
Author(s):  
Simona Prencipe ◽  
Ilenia Siciliano ◽  
Carlotta Gatti ◽  
Maria Gullino ◽  
Angelo Garibaldi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Fungal Growth ◽  
Aflatoxin Production ◽  
Aflatoxin Contamination ◽  
Total Phenol ◽  
Fungal Colonization ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Drying Treatment ◽  
Drying Conditions

Chestnut drying is used to prevent postharvest losses and microorganism contamination during storage. Several studies reported the contamination by aflatoxins (AFs) produced by Aspergillus spp. in chestnuts. The effect of drying temperatures (from 30 to 50 °C) was evaluated on the growth of A. flavus and the production of aflatoxins in chestnuts. The influence of the treatment on the proximate composition, the total phenol content and antioxidant activity of chestnuts was considered. Fungal colonization was observed on the nuts dried at 30, 35, and 40 °C; the incidence was lower at 40 °C. The highest concentrations of AFB1 and AFB2 were produced at 40 °C. No aflatoxins were detected at 45 or 50 °C. At 40 °C A. flavus was under suboptimal conditions for growth (aw 0.78), but the fungus was able to synthesize aflatoxins. As the temperatures applied increased, the total phenol content increased, while the antioxidant activity decreased. A drying treatment at 45 °C for seven days (aw 0.64) could be a promising method to effectively control both the growth of aflatoxigenic fungi and the production of aflatoxins. This study provides preliminary data useful to improve the current drying conditions used in chestnut mills, to reduce both fungal growth and aflatoxin production.

scholarly journals Antioxidant activity and total phenol content of blackberries cultivated in a highland tropical climate

2017 ◽  
Vol 39 (1) ◽  
pp. 43 ◽  
Author(s):  
Mayara Neves Santos Guedes ◽  
Rafael Pio ◽  
Luana Aparecida Castilho Maro ◽  
Fabíola Fonseca Lage ◽  
Celeste Maria Patto de Abreu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Phenolic Contents

Blackberries are an important option for the diversification of fruit crops. However, there is currently no literature regarding plant cultivation in high-altitude tropical climates. Knowledge of the phenolic composition of blackberries is essential because variations in the levels of these components may exist between cultivars and may depend on environmental conditions. High performance liquid chromatography (HPLC) was used to evaluate the total phenol content of different blackberry cultivars (Arapaho, Brazos, Cainguangue, Cherokee, Choctaw, Comanche, Ébano, Guarani, Tupy and Xavante). Free radical scavenging activity in these cultivars was assayed using a DPPH test. The HPLC-UV chromatogram of blackberry fruit extracts at 280 nm revealed the presence of phenolic compounds. The results showed significant differences in the levels of phenolic compounds in the blackberry cultivars tested. Antioxidant activity was evaluated using the ABTS free radical and ranged from 2.7 ± 0.1 to 19 ± 2 μmole of Trolox equivalents per gram of sample (b.u.). These results are in good correlation with the phenolic contents of the blackberries tested. The Xavante blackberry cultivar had the highest levels of polyphenols that could be individually identified. Catechin polyphenols were found to be the main component in the blackberry varieties tested. 

scholarly journals Changes in antioxidant activity and total phenol content during storage of sweet basil

2005 ◽  
Vol 31 (6) ◽  
pp. 315-317
Author(s):  
Arisa NOGUCHI ◽  
Shingo ITO ◽  
Tadashi BABA ◽  
Fukio IKEDA
Keyword(s):  
Antioxidant Activity ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Sweet Basil

scholarly journals Ascorbic Acid, Total Phenol Content and Antioxidant Activity of Fresh Juices of Four Ripe and Unripe Citrus Fruits

2012 ◽  
Vol 1 (2) ◽  
pp. 303-310 ◽  
Author(s):  
C. REKHA ◽  
G. POORNIMA ◽  
M. MANASA ◽  
V. ABHIPSA ◽  
J. PAVITHRA DEVI ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Citrus Fruits ◽  
Phenol Content

scholarly journals The Content of Chlorophyll, and Antioxidant Activity of Malabar plum (Syzygium jambos) Leaves at Different Developmental Stages

2019 ◽  
Vol 11 (2) ◽  
pp. 226-233
Author(s):  
Ikhsanti Maliya ◽  
Sri Darmanti ◽  
Sri Widodo Agung Suedy
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Developmental Stages ◽  
Ascorbic Acid Content ◽  
Total Phenol ◽  
Color Measurement ◽  
Total Phenol Content ◽  
Chlorophyll Contents ◽  
Syzygium Jambos ◽  
Dpph Method

Malabar plum [Syzygium jambos (L.) Alston.] is a tropical plant which is used as a medicinal plant, because it contains secondary metabolites, especially in the leaves. The different leaves developmental stages can affect physiological changes, especially metabolic processes, so it is suspected to affect the antioxidant content and activity. The objective of this research was to study the difference of leaves morphology, chlorophyll contents, antioxidant contents, and activity at the different leaves developmental stages. Samples were taken from Kaliboto Village, Purworejo, Central Java. The leaf color measurement was using colorimetry; determination of chlorophyll, carotenoids, ascorbic acid, and total phenol content is was using spectrophotometry; and antioxidant activity was using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Qualitative data were analyzed descriptively, while quantitative data were analyzed by ANOVA tests. The results showed that the higher level of leaves development, the higher pigment content, total phenol, and antioxidant activity ascorbic acid content in the mature leaves is lower when compared to the young and old leaves. The results of this research provide the information that can support the use of Malabar plum leaves in traditional medicinal activity and pharmaceutical industry, as well as basic information for plant breeding.

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