Anti-Hyperglycaemic Effect of Cleome Rutidosperma in Alloxan-Induced Diabetic Albino Rats

Drug Research ◽  
2017 ◽  
Vol 67 (12) ◽  
pp. 719-723
Author(s):  
Olayinka Oridupa ◽  
Naomi Ovwighose ◽  
Adebowale Saba
Keyword(s):  
Glycemic Control ◽  
Fasting Blood Glucose ◽  
Rapid Onset ◽  
Diabetic Rats ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Onset Of Action ◽  
Group B ◽  
Cleome Rutidosperma

AbstractThe hypoglycaemic and antihyperglycaemic effects of methanol extract of leaves of Cleome rutidosperma (Cr) DC (Family: Capparidaceae) was investigated in Wistar rats. Fifty normoglycaemic male rats (120 g–200 g) were divided into groups A (hypoglycaemic study; n=20) and B (antihyperglycaemic study; n=30). Each experiment had one control group and three groups administered with Cr (100, 200 or 400 mg/kg) respectively. Group B had two additional groups of diabetic-untreated rats and glibenclamide-treated diabetic rats. Diabetes was induced in Group B rats (except control) fasted overnight for 12 h by intraperitoneal injection of Alloxan (100 mg/kg). Fasting blood glucose levels (FBGL) were determined and alloxan-treated rats with BGL >200 mg/dl 48 h post-induction were considered diabetic. Data obtained were analyzed using One-way ANOVA and Duncan Multiple Range Test (p<0.05). Cr-treated rats showed significant decline in BGL with noteworthy decline by day 3 post-treatment at the dose of 200 mg/kg (236.40±14.72 mg/dl) from 336.40±21.06 mg/dl. Cr at the dose of 200 mg/kg (72.20±6.18 mg/dl, 69.20±7.81 mg/dl, 137.80±7.15 mg/dl and 70.60±10.66 mg/dl) showed better glycemic control compared to glibenclamide (194.50±7.75 mg/dl, 253.75±7.20 mg/dl, 284.25±10.56 mg/dl and 156.00±10.80 mg/dl). Cr-treated rats also showed progressive weight gain through the course of the study. This study demonstrated Cr has antihyperglycemic effect with more rapid onset of action and better glycemic control compared to glibenclamide.

scholarly journals Changes in Cardiac Levels of Caspase-8, Bcl-2 and NT-proBNP Following 4 Weeks of Aerobic Exercise in Diabetic Rats

2017 ◽  
Vol 2 (4) ◽  
pp. 172-177
Author(s):  
Saeid Tanoorsaz ◽  
Naser Behpoor ◽  
Vahid Tadibi
Keyword(s):  
Aerobic Exercise ◽  
Cardiac Tissue ◽  
Fasting Blood Glucose ◽  
Training Session ◽  
Exercise Program ◽  
Cardiovascular Complications ◽  
Diabetic Rats ◽  
Male Rats ◽  
Control Group ◽  
Caspase 8

Introduction: Cardiac apoptosis is one of the most important cardiovascular complications of diabetes. We aimed to investigate the changes of caspase-8, Bcl-2, and N-terminal pro B-type natriuretic peptide (NT-proBNP) in cardiac tissue after 4 weeks of aerobic exercise in male rats with diabetes. Methods: Forty adult male rats were randomly allocated to healthy control, diabetes, control + exercise and exercise + diabetes groups. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ) solution (55 mg/kg). Two weeks after injection, fasting blood glucose levels were measured. After the induction of diabetes, the exercise program was performed for 4 weeks (5 sessions per week) at a speed of 15 to 18 m/min for 25 to 44 minutes. Forty-eight hours after the last training session, the subjects were anesthetized and the heart muscle was removed. Caspase-8, Bcl-2 and NT-proBNP levels were measured by ELISA method. Results: The induction of diabetes in the control group resulted in a significant increase in caspase-8, and NT-proBNP levels while an insignificant increase was observed for Bcl-2 levels (P<0.05). In non-diabetic groups, exercise caused no changes in caspase-8, NT-proBNP and Bcl-2 (P<0.05). Exercise in diabetic groups significantly decreased NT-proBNP while no changes were observed in caspase-8 and Bcl-2 (P<0.05). Conclusion: Our findings showed that diabetes increases the pro-apoptotic and anti-apoptotic agent. In addition, 4 weeks of regular aerobic exercises can be used as a non-pharmacological strategy to reduce the complications of apoptosis in diabetic cardiomyocytes.

scholarly journals Anti-hyperglycemic Effects of Diacerein in Alloxan-Induced Diabetes Mellitus in Wistar Albino Rats

2020 ◽  
pp. 107-113
Author(s):  
Arsalan Uqaili ◽  
Samia Siddiqui ◽  
Roomi Aijaz ◽  
Yar Muhammad Nizammani ◽  
Navaid Kazi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Treated Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Group B ◽  
Group D

Objective: To determine the anti-hyperglycemic effects of interleukin-1 inhibitor (diacerein) in alloxan induced diabetic albino wistar rats. This experimental study was performed at the Department of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam within 6 months from April 2016 to September 2016. Total of 160 adult Albino Wistar Rats having an average of 200 to 300 grams body weights were selected. Animals were categorized into 4 groups as; Group A (n=15): Control rats – receive 0.9% normal saline as placebo Experimental Groups Group B (n=15): Experimental Control (Diabetic rats) - Alloxan50 mg/kg body weight intraperitoneal. Group C (n=15): Diabetic rats + Diacerein (30 mg/kg/day) orally daily. Group D (n=15): Diabetic rats + Diacerein (50 mg/kg/day) orally daily. Animals were kept and treated as per the NIH Guideline for Use and Care of Laboratory Animals. Diabetes mellitus was induced via a single intraperitoneal injection of 50 milligram/kg alloxan monohydrated dissolved in aseptic 0.9% saline. After 72 hours, blood specimens were taken from the caudal vein of the rats and glucose level>200 mg/dL was taken as diabetes. Experimental rats were given diacerein approximately 30 and 50 mg orally for 6 weeks. At the completion of experiment the body weight was measured of each animal by electronic measuring balance and blood sample was taken from each animal of all groups to assess the blood glucose level and HbA1c level. Data were recorded via self-made proforma and analysis was done by using SPSS version 20. Results: Average body weight of Diabetic control (Group B) was 193.33±22.50 grams, which was lower in contrast to Diacerein treated group C 202.47±25.70 grams and significantly lower as compared to Diacerein treated group D as  212.6±23.43 grams. A significant increase in blood glucose levels 182.07±10.63 mg/dl was noted in the Diabetic control (Group B) compared to Diacerein treated group C (110.13± 8.54 mg/dl) and group D (85.87±8.41 mg/dl) (P=0.001). HbA1c was markedly raised in the Group B- diabetic controls, while diacerein treated diabetic rats (groups C and D) showed a significant decrease in HbA1c (P=0.001). Conclusion: It was concluded that Diacerein achieves the Euglycemic state by reducing the levels of blood glucose and glycated hemoglobin (HbA1c) in Alloxan-Induced diabetes mellitus in Wistar Albino Rats.

scholarly journals Evaluation of Antidiabetic Potentials of Morus mesozygia Linn. Stapf., Leaf Extracts in Streptozotocin-Induced Diabetic Rats

2020 ◽  
pp. 23-32
Author(s):  
Marcella Tari Joshua ◽  
Edna O. Wachuku ◽  
N. Boisa ◽  
Nsirim Nduka
Keyword(s):  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Male Rats ◽  
Controlled Study ◽  
Albino Rats ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Citrate Buffer ◽  
Standard Drug ◽  
C Peptide

Aim: The aim of this study therefore was to evaluate the antidiabetic potentials of Morus mesozygia Linn. Stapf., Leaf extrcts in Streptozotocin-Induced Diabetic Rats. Study Design:  The study is an experimental case-controlled study. Place and Duration of Study: This study was carried out at the Biochemistry Research Laboratory, University of Port Harcourt, Rivers State, Nigeria, between June 2018-April 2019. Methodology: A total of 65 male albino rats that weighed between 150g to 200g were used for this research study. Three different extracted solvents; aqueous, ethanolic and methanolic leaves extracts were administered to different groups of the rats. The male albino rats for this study were induced with a single dose of 40mg/kg b.wt, intraperitoneally of streptozotocin in 0.1M of citrate buffer, pH 4.5. The diabetic male rats were those whose fasting blood glucose (FBG) was from 250 mg/dl or 13 mmol/L and above. Results: The results showed that there were significant decreases (p<0.05) in, FBG, increased insulin and increased C-peptide and increased HOMA-IR concentration of induced diabetic male rats treated orally with 400 mg/kg of MMLS., when compared with the values of the diabetic male rats in treated orally with 200 mg/kg of MMLS., and non-significant decrease(p<0.05) in FBG, HOMA-IR increased insulin, increased C-peptide concentrations in the diabetic male rats treated orally for 30 days with 400 mg/kg in dose of methanolic leaves extracts of MMLS., in the group treated with 400 mg/kg methanol, when compared to the concentration of diabetic male in treated orally for 30 days with 200 mg/kg of methanolic leaves extracts of MMLS. Compared likewise with that treated with 100mg/kg of metformin standard drug. There were also significant decrease (p<0.05) in FBG, HOMA-IR, C-peptide and increased insulin concentrations in diabetic male rats treated with 200 mg/kg in dose of ethanolic and methanolic leaves extracts of MMLS., when compared with the values in the diabetic male rats treated orally with 400 mg/kg in dose of ethanolic and methanolic leaves extracts of MMLS., compared with values of diabetic male rats treated orally with 100mg/kg in dose of metformin standard drugs compared with the controls. Conclusion: From the findings of this study, we conclude that streptozotocin increased FBG levels, while the Methanolic, ethanolic and aqueous extracts of Morus mesozygia Linn. S improved FBG, C-peptide, insulin and HOMA levels in a dose-dependent manner, with the methanolic extract having the best ameliorative effect, probably due to its more phytochemical composition.

scholarly journals Antidiabetic and Antioxidant Activities of the Twigs of Andrograhis paniculata on Streptozotocin-Induced Diabetic Male Rats

BioChem ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 238-249
Author(s):  
Olubanke O. Ogunlana ◽  
Babatunde O. Adetuyi ◽  
Elohor F. Esalomi ◽  
Miracle I. Rotimi ◽  
Jacob O. Popoola ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Antioxidant Activities ◽  
Fasting Blood Glucose ◽  
Male Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Oral Glucose ◽  
Distilled Water ◽  
Group 2

Background: Diabetes is associated with chronic hyperglycaemia, long-term damage, dysfunction, and organ failure. This study aims to evaluate the antidiabetic activity of the twigs of Andrographis paniculata and its toxicological markers on Streptozotocin (STZ)-induced diabetic Albino rats. Methods: A total of thirty rats were randomly divided into five groups of six animals each. Non-diabetic animals were treated with distilled water as non-diabetic sham control group 1, while diabetic animals (group 2, 3, 4 and 5) were treated with 60 mg/kg bw STZ intravenous (iv) and 100 mg/kg body weight (bwt) of metformin orally for group 2, distilled water for group 3, and 250 and 500 mg/kg bwt of Andrograhis paniculata (A. paniculata) for groups 4 and 5, respectively. The animals were dosed for 28 days, after which they were sacrificed. Liver and kidney function tests as well as livid profile tests were used as the biomarkers of toxicological assessment. Fasting blood glucose was carried out weekly. Oral Glucose Tolerance Test (OGTT) was conducted on the 28th day of the antidiabetic assessment. Results: A. paniculata groups 4 and 5 were significant at different doses (p < 0.05) in reducing the blood glucose level in comparison with metformin. There were significant changes in total and direct bilirubin, total protein, potassium, triglyceride and inorganic phosphorus in 500 mg/kg bwt of the treated group in comparison with the metformin and diabetic group groups. A. paniculata at 500 mg/kg bwt is most effective for its antidiabetic and organ protecting effects.

The Effect of Herbal Infusion on Fasting Blood Glucose (FBS) and Malondyaldehyde (MDA) Levels in Diabetic Rats

2020 ◽  
Vol 1009 ◽  
pp. 9-14
Author(s):  
Apriliani Ismi Fauziah ◽  
Lintang Dian Saraswati ◽  
Ari Udiyono ◽  
Beti Safitri
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Aloe Vera ◽  
Control Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats

Aloe vera, ginger, and sappan wood have recently gained attention as a potent antioxidant. This study was undertaken to analyze the possible effects of oral administration of herbal infusion (the combination of Aloe vera, ginger, and sapang wood) on fasting blood glucose (FBS) and malondialdehyde (MDA) levels in diabetic rats. Forty male albino rats (150-250 gm) were divided into five groups (n=8 in each group): alloxan-induced diabetic (negative control group/K-), acarbose treated (positive control group/K+), diabetic rats treated with herbal infusion dose 6.75 mg/150 gmbw (P1), diabetic rats treated with herbal infusion dose 13.5 mg/150 gmbw (P2), and diabetic rats treated with herbal infusion dose 20.25 mg/150 gmbw (P3). Male albino rats induced with a dose of alloxan 120 mg/kgbw and also 5% glucose after 6 hours of alloxan induction. FBS and MDA levels of each rat were measured before induction of alloxan was performed as a control sample. After seven days and 14 days of herbal infusion administration, FBS parameters was investigated with a glucometer. In the last week, the concentration of MDA in serum blood was determined using a UV-VIS spectrophotometer with a wavelength of 545 nm. Diabetic rats exhibited a significant decrease in FBS and MDA. Administration of herbal infusion was reduced FBS significantly between groups (ANOVA, Bonferroni, p<0.05). The concentration of MDA in serum blood was decreased significantly on K-, K+, P1, P2, P3 compared to the control group (ANOVA, LSD, P<0.05). It could be postulated herbal infusion has anti-hyperglycemic and anti-diabetic effects of FBS and MDA in male albino rats Wistar strain.

scholarly journals Antihyperglycaemic Effect of Tetracarpidium Conophorum Nuts in Alloxan Induced Diabetic Female Albino Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Donatus Onukwufor Onwuli ◽  
Holy Brown ◽  
Harrison Anaezichukwuolu Ozoani
Keyword(s):  
Blood Sugar ◽  
Fasting Blood Glucose ◽  
Test Group ◽  
Hemoglobin Concentration ◽  
Daily Basis ◽  
Negative Control ◽  
Diabetic Rats ◽  
The Body ◽  
Control Group ◽  
Albino Rats

The antihyperglycaemic activity of Tetracarpidium conophorum nut (walnut) was investigated in albino rats. A total of 20 albino rats were used for the study. The rats were divided into five groups (A–E) of four rats each. Diabetes were induced in the rats except four which served as the positive control group A. Groups B (negative control), C, D, and E contain diabetic rats each with blood sugar level ≥17.00 mmol/L. Groups A and B were fed on 85.2 g of top feed grower over the test period. Test groups C, D, and E were fed on 21.3 g, 42.6 g, and 85.2 g of walnuts, respectively, and their fasting blood sugar (FBS) levels were checked on daily basis. Fasting blood glucose levels of the test groups were significantly lower than negative control P<0.05, for 3rd, 7th, and 10th days of the test. There were also significant increase in the body weight and hemoglobin concentration and a decreased urine output of the test group compared with the controls. These results indicate that Tetracarpidium conophorum nut (walnut) has an antihyperglycemic effect in diabetic rats.

scholarly journals HYPERPLASTIC THYROID GLAND

2010 ◽  
Vol 17 (01) ◽  
pp. 21-30
Author(s):  
ANWAAR HUSSAIN ◽  
Nabeela Kaukab ◽  
SAIF-U- DIN ◽  
Muhammad Ismail Ismail ◽  
KHALID MEHMOOD AKHTAR
Keyword(s):  
Thyroid Gland ◽  
Thyroid Diseases ◽  
Male Rats ◽  
Medical Institute ◽  
Control Group ◽  
Albino Rats ◽  
Follicular Cells ◽  
Thiourea Group ◽  
Group A ◽  
Group B

Aim and Objective: The study was conducted to see the effects of dexamethasone (synthetic corticosteroid) on involution ofhyperplastic thyroid gland in albino rats, and to evaluate its uses in thyroid diseases. Study Design: Comparative histological study done inPostgraduate Medical Institute Lahore in 1998. Materials and methods: 54 adult male rats were taken and divided at random into control grouphaving 27 rats and experimental groups containing 27 rats. Control group was given normal diet along with 2 microgram of Potassium iodidefor 21 days while experimental groups further subdivided into group A having 3 rats and group B and C containing 12 rats each. All theexperimental groups were treated with Thiourea. Group A was sacrificed on day 22 after withdrawal of thiourea. Group B was given Potassiumiodide after stoppage of thiourea on day 21 and were sacrificed on days 22, 26, 30 and 50 in 4 sub groups (B1- B4). Group C was injectedDexamethasone daily from day 22 to day 50 and sacrificed on same days in 4 subgroups (C1-C2) to study involution process. Results:Histologically experimental group A exhibited significant increase in width and length of follicular cells lining the small sized follicles havingscanty colloid. The results of experimental subgroups B revealed early and complete involution whereas subgroups C showed significantlypersistent hyperplastic changes in the form of tall follicular cells lining small empty follicles. Conclusion: Thus it was concluded thatdexamehasone did retain hyperplastic changes during involution process, so steroid should consciously be used in thyroid diseases.

scholarly journals Assessment of Antioxidant Effects of Aqueous, Ethanolic and Methanolic Extracts of Morus mesozygia Linn. Stapf., Leaves in Streptozotocin-Induced Diabetic Rats

2020 ◽  
pp. 22-30
Author(s):  
Marcella Tari Joshua ◽  
Edna O. Nwachuku ◽  
N. Boisa ◽  
Nsirim Nduka
Keyword(s):  
Oxidative Stress ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Male Rats ◽  
Stress Index ◽  
Controlled Study ◽  
Albino Rats ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Antioxidant Effects

Aim: The aim of this study was therefore to assess the antioxidant effects of aqueous, ethanolic and methanolic extracts of Morus mesozygia Linn. Stapf., Leaves in Streptozotocin-Induced Diabetic Rats. Study Design:  The study is an experimental case-controlled study. Place and Duration of Study: This study was carried out at the Biochemistry Research Laboratory, University of Port Harcourt, Rivers State, Nigeria, between June 2018-April 2019. Methodology: A total of 65 male albino rats that weighed between 150g to 200g were used for this research study. Three different extracted solvents; aqueous, ethanolic and methanolic leaves extracts were administered to different groups of the rats. The male albino rats for this study were induced with a single dose of 40mg/kg b.wt, intraperitoneally of streptozotocin in 0.1M of citrate buffer, pH 4.5. The diabetic male rats were those whose fasting blood glucose (FBG) were from 250 mg/dl or 13 mmol/L and above. Results: The results showed that there were significant increases in the levels of superoxide dismutase(SOD,411.8±1.49) ng/ml, total antioxidant status (TAS,75.25±0.42) mU/ml, total oxidant status (TOS,353.51± 6.07) mU/ml activity, an oxidative stress index of 4.69±0.05 and a reduced concentration of malondialdehyde (MDA of 19.0± 1.49 mmol/L) when rats were treated with 400mg/kg of aqueous leaves of Morus mesozygia Linn. S., when compared with those of rats treated with 200mg/kg of aqueous leaf extracts of MMLS. Other methods of extractions (methanolic and ethanolic), also improved the antioxidant statuses of the diabetes induced and treated rats. Conclusion: Methanolic, ethanolic and aqueous extracts of Morus mesozygia Linn. S ameliorated oxidative stress, in Streptozotocin-induced diabetic rats, with the methanolic extract showing the most potent effect.

scholarly journals Study the Effects of Formaldehyde and Xylene Vapor on Lung and Testicular Tissue with Sperm Morphology of Adult Albino Rats

2021 ◽  
Vol 11 (1) ◽  
pp. 46-51
Author(s):  
Trefa F. Ismail ◽  
Galawezh O. Othman ◽  
Naznaz H. Othman ◽  
Ban A. Hassan
Keyword(s):  
Testicular Tissue ◽  
Chemical Substance ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
Cytotoxic Effects ◽  
Histological Techniques ◽  
Group A ◽  
Group B

Formalin is widely used chemical substance in industry and medicine (as tissue fixative and disinfectant). It contains reactive molecules which have been known for its cytotoxic effects. Furthermore, xylene used in histological techniques during preparation of tissue and has different toxic effects on tissues. The present study was performed to investigate the effects of formaldehyde and xylene inhalation on albino rat’s lung and testes. Fifteen adult male rats were used in this study; the rats were divided randomly into three groups (A, B, and C). Group (A) comprises the control group, whereas the rats in the Group (B) were exposed to 10% formaldehyde vapor and the rats from Group (C) were exposed to xylene vapor for 4 h/day for 5 days/week for 1 month. Histological alterations were observed in the lung of formalin and xylene exposed rats manifested by lymphatic infiltration, emphysema, congestion, dilation of blood vessels, and alveolar sac and also thinning in the wall of alveolar bronchiole. On the other hand, formaldehyde and xylene affected the testes tissue of treated rats including atrophy in the seminiferous tubule cells, edematous connective tissue, degenerative of spermatogenesis, depletion in germinal layer, existing of vacuoles in the seminiferous tubules, and decrease in the number of sperms in the lumen of seminiferous tubules. Furthermore, sperms abnormalities were seen. The data of our study concluded that inhalation the vapor of formaldehyde and xylene has degenerative effects on lungs and testes of the experimental rats as well as affecting on the spermatocytes and increased the frequency of sperm abnormalities.

Anti-diabetic activity of diphenhydramine in diabetic rats

2020 ◽  
Vol 11 (4) ◽  
pp. 5067-5070
Author(s):  
Pang Jyh Chayng ◽  
Nurul Ain ◽  
Kaswandi Md Ambia ◽  
Rahim Md Noah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Group Iv ◽  
Diabetic Rat ◽  
Control Group ◽  
Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p&lt;0.05) and group IV (11.34 ±3.67, p&lt;0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p&lt;0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

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