Evaluation of Cannabinoid and Terpenoid Content: Cannabis Flower Compared to Supercritical CO2 Concentrate

Planta Medica ◽  
2017 ◽  
Vol 84 (04) ◽  
pp. 234-241 ◽  
Author(s):  
Michelle Sexton ◽  
Kyle Shelton ◽  
Pam Haley ◽  
Mike West
Keyword(s):  
High Performance ◽  
The United States ◽  
Extraction Process ◽  
Complete Characterization ◽  
Gas Chromatography Mass Spectrometry ◽  
Array Detector ◽  
Health Related ◽  
Species Type ◽  
Relative Potencies

AbstractA recent cannabis use survey revealed that 60% of cannabis users rely on smelling the flower to select their cannabis. Olfactory indicators in plants include volatile compounds, principally represented by the terpenoid fraction. Currently, medicinal- and adult-use cannabis is marketed in the United States with relatively little differentiation between products other than by a common name, association with a species type, and Δ-9 tetrahydrocannabinol/cannabidiol potency. Because of this practice, how terpenoid compositions may change during an extraction process is widely overlooked. Here we report on a comparative study of terpenoid and cannabinoid potencies of flower and supercritical fluid CO2 (SC-CO2) extract from six cannabis chemovars grown in Washington State. To enable this comparison, we employed a validated high-performance liquid chromatography/diode array detector methodology for quantification of seven cannabinoids and developed an internal gas chromatography-mass spectrometry method for quantification of 42 terpenes. The relative potencies of terpenoids and cannabinoids in flower versus concentrate were significantly different. Cannabinoid potency increased by factors of 3.2 for Δ-9 tetrahydrocannabinol and 4.0 for cannabidiol in concentrates compared to flower. Monoterpenes were lost in the extraction process; a ketone increased by 2.2; an ether by 2.7; monoterpene alcohols by 5.3, 7 and 9.4; and sesquiterpenes by 5.1, 4.2, 7.7, and 8.9. Our results demonstrate that the product of SC-CO2 extraction may have a significantly different chemotypic fingerprint from that of cannabis flower. These results highlight the need for more complete characterization of cannabis and associated products, beyond cannabinoid content, in order to further understand health-related consequences of inhaling or ingesting concentrated forms.

scholarly journals Investigation of the Antifungal and Anti-Aflatoxigenic Potential of Plant-Based Essential Oils against Aspergillus flavus in Peanuts

2020 ◽  
Vol 6 (4) ◽  
pp. 383
Author(s):  
Premila Narayana Achar ◽  
Pham Quyen ◽  
Emmanuel C. Adukwu ◽  
Abhishek Sharma ◽  
Huggins Zephaniah Msimanga ◽  
...  
Keyword(s):  
Essential Oils ◽  
Aspergillus Flavus ◽  
High Performance ◽  
Opportunistic Infections ◽  
Aflatoxin Production ◽  
The United States ◽  
Microscopy Study ◽  
Aspergillus Species ◽  
Gas Chromatography Mass Spectrometry ◽  
Ammonia Vapor

Aspergillus species are known to cause damage to food crops and are associated with opportunistic infections in humans. In the United States, significant losses have been reported in peanut production due to contamination caused by the Aspergillus species. This study evaluated the antifungal effect and anti-aflatoxin activity of selected plant-based essential oils (EOs) against Aspergillus flavus in contaminated peanuts, Tifguard, runner type variety. All fifteen essential oils, tested by the poisoned food technique, inhibited the growth of A. flavus at concentrations ranging between 125 and 4000 ppm. The most effective oils with total clearance of the A. flavus on agar were clove (500 ppm), thyme (1000 ppm), lemongrass, and cinnamon (2000 ppm) EOs. The gas chromatography-mass spectrometry (GC-MS) analysis of clove EO revealed eugenol (83.25%) as a major bioactive constituent. An electron microscopy study revealed that clove EO at 500 ppm caused noticeable morphological and ultrastructural alterations of the somatic and reproductive structures. Using both the ammonia vapor (AV) and coconut milk agar (CMA) methods, we not only detected the presence of an aflatoxigenic form of A. flavus in our contaminated peanuts, but we also observed that aflatoxin production was inhibited by clove EO at concentrations between 500 and 2000 ppm. In addition, we established a correlation between the concentration of clove EO and AFB1 production by reverse-phase high-performance liquid chromatography (HPLC). We demonstrate in our study that clove oil could be a promising natural fungicide for an effective bio-control, non-toxic bio-preservative, and an eco-friendly alternative to synthetic additives against A. flavus in Georgia peanuts.

scholarly journals Bioproducts characterization of residual periphytic biomass produced in an algal turf scrubber (ATS) bioremediation system

2020 ◽  
Vol 82 (6) ◽  
pp. 1247-1259
Author(s):  
Maiara P. de Souza ◽  
Tiele M. Rizzetti ◽  
Michele Hoeltz ◽  
Mainara Dahmer ◽  
João A. Júnior ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
High Performance ◽  
Optical Emission ◽  
Algal Turf ◽  
Gas Chromatography Mass Spectrometry ◽  
Emission Spectrometry ◽  
Inorganic Analysis ◽  
Array Detector ◽  
Algal Turf Scrubber ◽  
Social Gain

Abstract The transformation of residual biomass from bioremediation processes into new products is a worldwide trend driven by economic, environmental and social gain. The present study aimed to evaluate the potential for obtaining bioproducts of technological interest from the remaining periphytic biomass formed during a bioremediation process with an algal turf scrubber (ATS) system installed in a lake catchment. Different methodologies were used according to the target bioproduct. Analyses were performed by high performance liquid chromatography with diode array detector (HPLC/DAD), gas chromatography mass spectrometry (GC-MS), ultraviolet–visible spectroscopy (UV-VIS) and inductively coupled plasma optical emission spectrometry (ICP-OES). The results demonstrated that the periphytic biomass presented potential since protein (17.7%), carbohydrates (22.4%), total lipids (3.3%) with 3.6 mg mL−1 of fatty acids, antioxidants (144.5 μmol Trolox eq. g−1) and chlorophyll a, chlorophyll b and carotenoids (1,719.7 μg mL−1, 541.2 μg mL−1 and 317.7 μg mL−1, respectively) were obtained. Inorganic analysis presented a value of 42.3 ± 2.58% of total ash and metal presence was detected, indicating bioaccumulation. The properties found in periphyton strengthen the possibility of its application in different areas, ensuring bioremediation efficiency.

scholarly journals PSIII-23 Nutrient and Maillard reaction product concentrations of commercially available pet foods and treats

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 273-274
Author(s):  
Patricia M Oba ◽  
Nagait Hwisa ◽  
Xinhe Huang ◽  
Keith Cadwallader ◽  
Kelly S Swanson
Keyword(s):  
Maillard Reaction ◽  
High Performance ◽  
Protein Quality ◽  
Daily Intake ◽  
The United States ◽  
Gas Chromatography Mass Spectrometry ◽  
Maillard Reaction Products ◽  
Reaction Products ◽  
Total Dietary Fiber ◽  
Randomized Design

Abstract Exposing ingredients to high temperatures may result in chemical reactions between amino acids and sugars, leading to the formation of Maillard reaction products (MRPs). These products may result in altered palatability, protein quality, and digestibility. This experiment quantified key MRPs in commercial pet foods, estimated daily intake of MRPs of pet animals, and correlated MRPs with macronutrient concentrations present in the foods. The analysis included 53 pet foods and treats commercially available in the United States: 11 dry dog foods, 29 wet dog foods, 2 dry dog treats, 3 wet dog treats, 2 dry cat foods, and 6 wet cat foods. Diets and treats were analyzed for dry matter, organic matter, crude protein (CP), acid-hydrolyzed fat, total dietary fiber, and gross energy using standard techniques. MRPs were analyzed using high-performance liquid chromatography and gas chromatography-mass spectrometry. All data were analyzed as a completely randomized design using the GLM procedure of SAS 9.4. On average, wet foods had higher (P < 0.001) fructoselysine (FRUC) and carboxymethyllysine (CML) than dry foods. Dry dog treats had the highest (P < 0.001) FRUC (29.64 µg/g) and CML (0.47 mg/g) concentrations of all products tested. Dry cat foods had the lowest (P < 0.001) CML (0.10 mg/g) concentrations of all commercial segments. Wet dog treats had the highest (P < 0.001) 5-hydroxymethyl-2-furfural (HMF) (10.92 µg/g) concentrations of all segments and wet cat foods had the lowest (P < 0.001) HMF (0.22 µg/g) content. Dogs and cats fed wet diets would be more likely to ingest higher amounts of CML and FRUC compared to animals fed dry diets. Dogs consuming dry treats would be more likely to ingest higher amounts of CML than wet treats. CP was negatively correlated to HMF (P = 0.0425; r = -0.27973), while TSF was positively correlated to CML (P = 0.0417; r = 0.28080).

scholarly journals Target and Suspect HRMS Metabolomics for the Determination of Functional Ingredients in 13 Varieties of Olive Leaves and Drupes from Greece

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4889 ◽  
Author(s):  
Evangelia Kritikou ◽  
Natasa P. Kalogiouri ◽  
Lydia Kolyvira ◽  
Nikolaos S. Thomaidis
Keyword(s):  
High Performance ◽  
Regression Coefficient ◽  
Good Precision ◽  
Olive Leaves ◽  
Functional Ingredients ◽  
Precision Limit ◽  
Health Related ◽  
Improve Health

The huge interest in the health-related properties of foods to improve health has brought about the development of sensitive analytical methods for the characterization of natural products with functional ingredients. Greek olive leaves and drupes constitute a valuable source of biophenols with functional properties. A novel ultra-high-performance liquid chromatography–quadrupole time of flight tandem mass spectrometry (UHPLC-QTOF-MS) analytical method was developed to identify biophenols through target and suspect screening in Greek olive leaves and drupes of the varieties: Koroneiki, Throumbolia, Konservolia, Koutsourelia, Kalamon, Petrolia, Amigdalolia, Megaritiki, Mastoeidis, Agouromanakolia, Agrilia, Adramitiani and Kolovi. The method’s performance was evaluated using the target compounds: oleuropein, tyrosol and hydroxytyrosol. The analytes demonstrated satisfactory recovery efficiency for both leaves (85.9–90.5%) and drupes (89.7–92.5%). Limits of detection (LODs) were relatively low over the range 0.038 (oleuropein)–0.046 (hydroxytyrosol) and 0.037 (oleuropein)–0.048 (hydroxytyrosol) for leaves and drupes, respectively For leaves, the precision limit ranged between 4.7% and 5.8% for intra-day and between 5.8% and 6.5% for inter-day experiments, and for drupes, it ranged between 3.8% and 5.2% for intra-day and between 5.1 and 6.2% for inter-day experiments, establishing the good precision of the method. The regression coefficient (r2) was above 0.99 in all cases. Furthermore, the preparation of herbal tea from olive leaves is suggested after investigating the optimum infusion time of dried leaves in boiling water. Overall, 10 target and 36 suspect compounds were determined in leaves, while seven targets and thirty-three suspects were identified in drupes, respectively.

scholarly journals Separation and HPLC Characterization of Active Natural Steroids in a Standardized Extract from the Serratula coronata Herb with Antiseborrheic Dermatitis Activity

2020 ◽  
Vol 17 (18) ◽  
pp. 6453
Author(s):  
Marta Napierała ◽  
Joanna Nawrot ◽  
Justyna Gornowicz-Porowska ◽  
Ewa Florek ◽  
Arletta Moroch ◽  
...  
Keyword(s):  
High Performance ◽  
Seborrheic Dermatitis ◽  
Array Detector ◽  
Diode Array Detector ◽  
Therapeutic Benefits ◽  
Serratula Coronata ◽  
Positive Effect ◽  
Active Natural

Phytoecdysteroids are natural compounds with therapeutic benefits in both humans and animals. The effectiveness of natural products with health potential is based on the activities and potencies of their active ingredients. In this study, dominant ecdysteroids—ajugasterone C, 20-hydroxyecdysone and polypodine B—from the Serratula coronata (S. coronata) herb were separated by column chromatography, identified by spectroscopic data and quantified by high-performance liquid chromatography with a diode array detector (HPLC-DAD). The obtained concentration of ecdysteroids (approximately 23%) in the S. coronatae herb extract enhances the possibility of their use in pharmaceutical and cosmetic products with high levels of phytoecdysteroids. Moreover, this study has shown a positive effect of ecdysteroids-containing cream on changes in quality of life and a beneficial effect in reducing the symptoms of seborrheic dermatitis. It has been demonstrated that the application of the cream with phytoecdysteroids resulted in a statistically significant alleviation of symptoms (p < 0.05), especially in terms of itching, pain or burning sensations in the affected areas in comparison to previous symptoms.

Characterization of phenolic compounds from inner bark of Betula pendula

Holzforschung ◽  
2012 ◽  
Vol 66 (2) ◽  
Author(s):  
Jaana Liimatainen ◽  
Maarit Karonen ◽  
Jari Sinkkonen ◽  
Marjo Helander ◽  
Juha-Pekka Salminen
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Betula Pendula ◽  
Silver Birch ◽  
Biologically Active ◽  
Array Detector ◽  
Ionization Mass ◽  
Inner Bark ◽  
First Time

Abstract A method has been developed for the characterization of biologically active silver birch (Betula pendula) inner bark phenolics based on high-performance liquid chromatography/diode array detector (HPLC-DAD)/electrospray ionization-mass spectrometry (ESI-MS). It was demonstrated that the inner bark contains high amounts of flavonoids, arylbutanoids, diarylheptanoids, simple phenolic compounds, phenolic acids, lignans, and procyanidins. Altogether, 30 individual compounds were characterized based on their ultraviolet (UV) and MS data. Structures of 22 compounds were confirmed by nuclear magnetic resonance (NMR) spectroscopy. In addition to previously reported phenolic compounds, 12 compounds were identified in silver birch inner bark for the first time; two of them are novel compounds: 3-β-glucopyranosyloxy-2-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)-propan-1-one and 1,7-bis-(4-hydroxyphenyl)-3-heptanol 3-O-β-ap-iofuranosyl-(1→2)-β-glucopyranoside.

scholarly journals Chemical characterization of an Ipecac active germplasm bank – a Brazilian endangered medicinal species

2021 ◽  
Vol 10 (15) ◽  
Author(s):  
Nádia Elígia Nunes Pinto Paracampo ◽  
Luiz Ricardo Santos de Souza ◽  
Jéssica Caporal Almeida ◽  
Osmar Alves Lameira
Keyword(s):  
High Performance ◽  
Genetic Material ◽  
Chemical Characterization ◽  
Array Detector ◽  
Germplasm Bank ◽  
Medicinal Species ◽  
Breeding Programs ◽  
The City ◽  
Selection Of

The active germplasm bank (AGB) of Carapichea ipecacuanha (Brot.) L. Andersson at Embrapa Eastern Amazon, in the city of Belém, PA, was the first of its kind to be opened in Brazil for this endangered medicinal species and holds important accessions for agricultural and reproductive research, including the production of active principles. This study aimed to chemically characterize 42 accessions from that AGB by simultaneously quantifying cephaeline and emetine contents in roots using high-performance liquid chromatography with a diode-array detector (HPLC-DAD). Cephaeline concentrations ranged from ‘undetected’ to 1.76%, whereas emetine concentrations were found between 0.64% and 2.49%. The overall emetine/cephaeline ratio varied from 0.43 to 3.52. The differences among mean concentrations of alkaloids observed by comparing the Scott-Knott test at 5% probability suggest the chemical variation among the samples assessed. Therefore, it is concluded such chemical differences may favor the selection of genetic material for commercial purposes based on the production of emetine and/or cephaeline, likewise may contribute to breeding programs.

scholarly journals Laboratory characterization of Cor-Tuf Baseline and UHPC-S

2021 ◽  
Author(s):  
Dylan Scott ◽  
Steven Graham ◽  
Bradford Songer ◽  
Brian Green ◽  
Michael Grotke ◽  
...  
Keyword(s):  
High Performance ◽  
High Performance Concrete ◽  
The United States ◽  
Design Guidelines ◽  
Ultra High Performance Concrete ◽  
Laboratory Characterization ◽  
Potential Benefits ◽  
The Republic ◽  
Protective Structures

This experimental effort is part of a larger program entitled Development of Ultra-High-Performance Concrete Tools and Design Guidelines. This program operates in accordance with an agreement concerning combating terrorism research and development between the United States of America Department of Defense and the Republic of Singapore Ministry of Defence. The objective of the program is to develop a better understanding of the potential benefits that may be achieved from the application of ultra-high-performance concrete (UHPC) materials for protective structures. The specific effort detailed in this report will provide insight into laboratory-scale mechanical properties of Cor-Tuf and a proprietary material termed UHPC-Singapore (UHPC-S).

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