Yield improvement in high lysine barley

1981 ◽  
Vol 32 (3) ◽  
pp. 425 ◽  
Author(s):  
RN Oram ◽  
H Doll

Twenty-three barley genotypes were grown in field plots and the seed-yield components and seed composition were investigated. Ten malting and feed cultivars and waxy endosperm selection lines had the standard concentration of lysine in their seed protein. The other genotypes were five different natural or induced high-lysine mutants and/or selection lines developed from them. High-lysine lines were lower yielding than the most productive normal lysine cultivars because of a decrease in weight per seed, accompanied by a reduced content of the carbohydrates extractable with dimethyl sulfoxide. Ris� mutant no. 7 was exceptional in having fewer seeds per unit area and a normal carbohydrate content. Within the group of lys 3 homozygotes derived from Ris� no. 1508, the higher yielding lines had more seeds per unit area, higher concentrations of extractable carbohydrates, and lower concentrations of crude protein. Similar trends with yield also occurred among the lines homozygous for the lys gene from Hiproly. The yields of the most productive lys and lys 3 homozygotes approached that of Clipper, the leading Australian malting cultivar, but were 25% lower than that of the most productive feed barley, Cutter. The other mutants, Ris� no. 7, Ris� no. 29 and Clipper 500, were lower yielding than the better lys and lys 3 selections. The possibilities for improving the yield and energy content of the latter two high-lysine types by selection are discussed.

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1254
Author(s):  
Marcus Jones ◽  
Marin Harbur ◽  
Ken J. Moore

Plot size has an important impact on variation among plots in agronomic field trials, but is rarely considered during the design process. Uniformity trials can inform a researcher about underlying variance, but are seldom used due to their laborious nature. The objective of this research was to describe variation in maize field trials among field plots of varying size and develop a tool to optimize field-trial design using uniformity-trial statistics. Six uniformity trials were conducted in 2015–2016 in conjunction with Iowa State University and WinField United. All six uniformity trials exhibited a negative asymptotic relationship between variance and plot size. Variance per unit area was reduced over 50% with plots 41.8 m2 in size and over 75% when using a plot size >111.5 m2 compared to a 13.9 m2 plot. Plot shape within a fixed plot size did not influence variance. The data illustrated fewer replicates were needed as plot size increased, since larger plots reduced variability. Use of a Shiny web application is demonstrated that allows a researcher to upload a yield map and consider uniformity-trial statistics to inform plot size and replicate decisions.


Genetics ◽  
1996 ◽  
Vol 144 (1) ◽  
pp. 329-338 ◽  
Author(s):  
R C Shoemaker ◽  
K Polzin ◽  
J Labate ◽  
J Specht ◽  
E C Brummer ◽  
...  

Abstract Restriction fragment length polymorphism mapping data from nine populations (Glycine max × G. soja and G. max × G. max) of the Glycine subgenus soja genome led to the identification of many duplicated segments of the genome. Linkage groups contained up to 33 markers that were duplicated on other linkage groups. The size of homoeologous regions ranged from 1.5 to 106.4 cM, with an average size of 45.3 cM. We observed segments in the soybean genome that were present in as many as six copies with an average of 2.55 duplications per segment. The presence of nested duplications suggests that at least one of the original genomes may have undergone an additional round of tetraploidization. Tetraploidization, along with large internal duplications, accounts for the highly duplicated nature of the genome of the subgenus. Quantitative trait loci for seed protein and oil showed correspondence across homoeologous regions, suggesting that the genes or gene families contributing to seed composition have retained similar functions throughout the evolution of the chromosomes.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhijuan Chen ◽  
Vanessa Lancon-Verdier ◽  
Christine Le Signor ◽  
Yi-Min She ◽  
Yun Kang ◽  
...  

AbstractGrain legumes are highly valuable plant species, as they produce seeds with high protein content. Increasing seed protein production and improving seed nutritional quality represent an agronomical challenge in order to promote plant protein consumption of a growing population. In this study, we used the genetic diversity, naturally present in Medicago truncatula, a model plant for legumes, to identify genes/loci regulating seed traits. Indeed, using sequencing data of 162 accessions from the Medicago HAPMAP collection, we performed genome-wide association study for 32 seed traits related to seed size and seed composition such as seed protein content/concentration, sulfur content/concentration. Using different GWAS and postGWAS methods, we identified 79 quantitative trait nucleotides (QTNs) as regulating seed size, 41 QTNs for seed composition related to nitrogen (i.e. storage protein) and sulfur (i.e. sulfur-containing amino acid) concentrations/contents. Furthermore, a strong positive correlation between seed size and protein content was revealed within the selected Medicago HAPMAP collection. In addition, several QTNs showed highly significant associations in different seed phenotypes for further functional validation studies, including one near an RNA-Binding Domain protein, which represents a valuable candidate as central regulator determining both seed size and composition. Finally, our findings in M. truncatula represent valuable resources to be exploitable in many legume crop species such as pea, common bean, and soybean due to its high synteny, which enable rapid transfer of these results into breeding programs and eventually help the improvement of legume grain production.


1972 ◽  
Vol 52 (4) ◽  
pp. 681-687 ◽  
Author(s):  
R. J. BOILA ◽  
T. J. DEVLIN

Four dairy steers were allotted to four lysine infusion levels in a 4 × 4 latin square design and fed an 11.5% crude protein (90% dry matter (DM)) diet continuously (10-min intervals every 24 hr). Lysine hydrochloride equivalent to 0.0, 3.0, 6.0, and 9.0 g lysine per day was infused per abomasum. When 9 g lysine were infused per day, the percent of absorbed nitrogen (N) retained was significantly (P < 0.05) reduced; urinary N excretion as a percentage of N intake and plasma-free lysine were increased significantly compared with the other three infusion treatments. The infusion of 9 g lysine per day apparently exceeded the body tissue requirements for this amino acid and the excess N was excreted in the urine. A possibility of lysine being limiting (0.28% lysine of a 100% DM diet) was apparently offset by the synthesis of lysine by rumen microorganisms, which increased the dietary lysine two- to threefold. Increased levels of infused lysine did not result in a linear increase of lysine in the abomasum. With 3 g per day lysine infusion rumen ammonia and N retentions were high. However, a smaller amount of N reached the abomasum with steers on this treatment.


Author(s):  
Marika Vogelzang

In this study I determined the effectiveness of pollinator gardens by testing if visitation rate and diversity of flower-visiting insects is higher in pollinator gardens compared to other ornamental plantings. I observed pollinator visitation for individual plant taxa, per unit area, in three different pollinator gardens, eight ornamental gardens and eight ornamental planters on the Queen’s University campus in Kingston, Ontario, Canada. Visitation was about 4- times higher in pollinator gardens compared to the other two ornamental garden types and visitor richness (the number of types of pollinators) in pollinator gardens was about 6- times higher compared to ornamental gardens, and about 3- times higher compared toornamental planters. The results of this study conclude that the planting of pollinator gardens is an effective way of supporting pollinator populations in urban areas.


2017 ◽  
Vol 130 (11) ◽  
pp. 2315-2326 ◽  
Author(s):  
Lillian F. Brzostowski ◽  
Timothy I. Pruski ◽  
James E. Specht ◽  
Brian W. Diers

1959 ◽  
Vol 52 (2) ◽  
pp. 230-237 ◽  
Author(s):  
D. J. C. Jones

1. Four varieties of marrow stem kale, three of thousand headed kales, Hungry Gap Kale and Rape-Kale were grown at three centres in mid-Wales. They were sampled in the early winter period and separate leaf and stem samples analysed for the proximate constituents and minerals.2. Leaf to stem ratios were measured on both a green and dry matter basis and the latter values used to calculate whole plant values for the chemical constituents.3. The leaves of the marrow stem kales were higher in dry matter than the stems, except in the case of the Purple Stem Kale. This variety resembled the thousand headed and rape-type kales in having a higher dry matter in the stems. On a dry matter basis, the leaves of all the varieties were richer than the stems in ether extract, crude protein, total ash and all the minerals except potassium and sodium. These elements occurred to a greater extent in the stems of the fleshy stemmed marrow stem kales than in the leaves. The stems were higher in crude fibre and nitrogen-free extractives.4. The leaves of the rape-type kales were higher than those of the other varieties in silica, phosphorus and potassium. They were the best source of phosphorus on a whole plant basis. The marrow stem kales had considerably higher leaf and stem values for sodium and were markedly superior to the other varieties as a source of the element.


Author(s):  
M. Cropper ◽  
M. Lloyd ◽  
G.C. Emmans ◽  
C.E. Hinks

The experiment described here was designed to test the proposition that lambs, given access to two feeds, as a choice, one abundant in crude protein (CP), the other deficient, can both select a diet which supports their potential growth rate and which avoids excesses of protein intake.Twenty four Suffolk x Greyface wether and females, at a mean liveweight of 20.2 kg (s.e. 0.6) were individually housed and their feed intake and liveweight recorded weekly.The four pelleted feeds used were highly digestible and had estimated energy yields of 10.5 MJ ME/kg freshweight. The basal feed (A) was made from equal parts barley and sugar beet pulp plus a vitamin/mineral mix. It contained 91 g CP/kg FW. Feed D was made by substituting fishmeal for the barley in feed A such that it contained 383g CP/kg. Feed B contained one third D and two thirds A and contained 182g CP/kg. Féed C contained two thirds D and one third A, giving a CP content of 266g CP/kg. Thus each of the four feeds A, B, C and D could be described in terms of either the proportion of feed A or the crude protein which it contained.Four lambs were offered feed A alone, and four lambs received feed B only. Twelve of the lambs were given ad 1ibitum access to both the basal feed A and one of the three other feeds B, C and D, for ten weeks. Four lambs were offered a choice between feed B and feed D for four weeks.


1998 ◽  
Vol 7 (3) ◽  
pp. 381-390 ◽  
Author(s):  
Jarmo Valaja ◽  
Hilkka Siljander-Rasi

A digestibility and balance trial was carried out with four intact castrated male pigs (live weight 33-82 kg) to study the effects of dietary crude protein and energy content on nutrient digestibility, nitrogen metabolism, water intake and urinary output. In a 4 x 4 Latin square design, four barley-oats-soya bean meal based diets were arranged 2x2 factorially. The corresponding factors were dietary crude protein (CP) content: high (180 g/kg CP) or low protein diet (140 g/kg CP) supplemented with free lysine, methionine and threonine; and dietary net energy content; high (1.05 feed units (FU)/kg) (feed unit=9.3 MJ net energy) or low net energy content (0.95 FU/kg). Lowering dietary CP content (mean values of 189 to 152 g/kg dry matter, respectively) by supplementation of free amino acids decreased urinary nitrogen (N) excretion by 6.9 g/day (32%) (P


Author(s):  
Oyetade Joshua Akinropo ◽  
Bello Lukman Abidemi ◽  
Adesiyan Blessing Adedayo

The proximate analysis of the fruit sample that homogenously sampled from the wild was taken and quartered to get appreciable weight fit for analysis. Due to it perishable nature the quartered sample for each of the fruit was stored in an air tight container and kept in the refrigerator at a temperature of about 4°C. For the determination of the nutritional composition, parameters which include their proximate, minerals, and vitamin C were quantitatively determined while the anti-nutrient composition were qualitatively and quantitatively analyzed. Compared to ackee’s apple, monkey cola was found to consist of 64.41% moisture content, 1.69% ash, 10.21% crude fibre, 1.25% crude fat, 4.44% crude fibre, 18.06% carbohydrates while ackee’s apple consist of 73.21% moisture, 1.49% ash, 9.38 % crude protein, 13.98% crude fat, 2.08% crude fibre, 0.86% carbohydrates and 4.45% of vitamin C. The mineral analysis for both samples was quantitatively determined using Atomic Absorption spectrophotometer (AAS). The minerals determined for ackee’s apple and monkey cola were magnesium which was 1391.65 ppm, calcium 628.23 ppm, sodium 506.96 ppm, potassium 3976.14 ppm, iron 1.0 ppm, copper 5.00, zinc 4.00, cobalt 3.0 and phosphorus 2616.90 ppm the iron however, was not detected. Ackee’s apple on the other hand, consist of magnesium 498.01 ppm, calcium 478.56 ppm, sodium 398.80 ppm, potassium 4970.18 ppm, copper 2.00, zinc 5.00, cobalt 3.0 and phosphorus 373.84 ppm. The qualitative screening of the anti-nutrients revealed the absence of phenol from both fruit samples while tannins were present only in the monkey cola. The flavonoids, phytic acid and oxalate were quantitatively determined to be 1240 mg, 625 mg and 155 mg for Monkey kola and 640 mg, 340 mg and 65 mg for Ackee’s apple.


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