Observations on the adaptation by sheep to saline drinking water

1967 ◽  
Vol 7 (27) ◽  
pp. 321 ◽  
Author(s):  
AD Wilson
Keyword(s):  
Drinking Water ◽  
Food Intake ◽  
Fresh Water ◽  
Dry Matter ◽  
In Vitro Digestion ◽  
Stable Level ◽  
Micro Organisms

The extent of adaptation by sheep and rumen micro-organisms to saline drinking water was investigated in two experiments. In the first experiment, eleven sheep were introduced suddenly to drinking water containing 2 per cent NaCl. Food intakes fell sharply for three days, but then increased again, and by five to seven days reached a stable level 160 g below the food intake on fresh water. In the second experiment, rumen liquor for in vitro digestion studies was taken from sheep conditioned to saline drinking water (2 per cent NaCl) and from sheep with no experience of saline drinking water. The amount of dry-matter (ryegrass) digested decreased with increasing concentration of the medium, but there was no evidence of adaptation by the rumen micro-organisms to this increased concentration. When the concentration was increased by the addition of NaCl alone, the decrease in amount digested was less than when the concentration of all ions was increased.

Influence of the concentration and volume of saline water on the food intake of sheep, and on their excretion of sodium and water in urine and faeces

1973 ◽  
Vol 24 (2) ◽  
pp. 245 ◽  
Author(s):  
AD Wilson ◽  
ML Dudzinski
Keyword(s):  
Drinking Water ◽  
Sodium Chloride ◽  
Food Intake ◽  
Fresh Water ◽  
Dry Matter ◽  
Saline Water ◽  
Sodium Content ◽  
Merino Sheep ◽  
C 50 ◽  
Sodium And Potassium

The intake of food and the excretion of urine and faeces by 15 Merino sheep were recorded while they were given fixed volumes of fresh or saline (1 -5 and 2.0% sodium chloride) drinking water. The food intakes of the sheep increased as the volumes of either fresh or saline water were increased from 0.5 to 6.0 l/day. The addition of sodium chloride to the water decreased the food intake, but the decrease was restored by an increase in the volume of water given of c. 50 % for 1.5 % sodium chloride, and c. 100% for 2.0% sodium chloride. However, on 2.0% sodium chloride, no further increase in the intake of food occurred with volumes above 3.0 l/day, and maximum intakes were only 6045% of the maximum intakes on fresh water. When the lower volumes of saline water were given, the amount of food eaten for a given volume of water was decreased at higher temperatures and slightly decreased by a higher potassium content of the diet. High volumes (4.0-6.0 l/day) of 2.0% sodium chloride depressed food intake when the ration was oaten chaff, but not when it was lucerne chaff. The increased intake of sodium arising from the addition of sodium chloride to the water was excreted in the urine, and there was no change in the sodium content of the faeces. The concentrations of sodium and potassium in the urine declined as the amount to be excreted was increased. The volume of urine excreted was related to the amount of sodium and potassium to be excreted, while the amount of water lost in the faeces was related to the quantity of faecal dry matter excreted and the type of diet.

scholarly journals 127 Porcine in vitro digestibility of whole stillage predigested with multi-enzyme during different time periods

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 49-50
Author(s):  
Kevin S Jerez Bogota ◽  
Tofuko A Woyengo
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Enzyme Treatment ◽  
Optimal Time ◽  
In Vitro Digestibility ◽  
Percentage Points ◽  
Whole Stillage ◽  
Freeze Dried ◽  
Different Sources

Abstract A study was conducted to determine the effects of the period of predigesting whole stillage (WS; slurry material that is dried into DDGS) with multi-enzyme and composition of the multi-enzyme on porcine in vitro digestibility of dry matter (IVDDM) of the WS. Four samples of whole stillage from 4 different sources were freeze-dried and divided into 13 subsamples to give 52 sub-samples. Thirteen treatments were applied to the 48 sub-samples within source. The treatments were undigested WS (control); or pre-digested with 1 of 3 multi-enzymes (MTE1, MTE2, and MTE3) at 55 °C for 6, 12, 18 or 24 h in 3 × 4 factorial arrangement. The MTE1 contained xylanase, β-glucanase, cellulase, mannanase, protease, and amylase; MTE2 contained xylanase, α-galactosidase, and cellulase; and MTE3 contained xylanase, cellulase, β-glucanase, and mannanase. The 52 subsamples were subjected to porcine in vitro digestion. The IVDDM of untreated WS was 73.3%. The IVDDM increased (P< 0.05) with an increase in the predigestion period. However, a rise in the predigestion period from 0 to 12 h resulted in greater (P< 0.05) response in mean IVDDM than an increment in the predigestion period from 12 to 24 h (11 vs. 0.83 percentage points). Predigestion period and multi-enzyme type interacted on IVDDM such that the improvement in IVDDM between 0 and 12 hours of predigestion differed (P< 0.05) among the 3 multi-enzyme types (13.3, 11.1, and 8.5 percentage points for MTE3, MTE2, and MTE1, respectively). The LS means by multi-enzyme treatment were modeled and resulted in unparallel curves (P< 0.05). The estimated maximum response of IVDDM for MTE1, MTE2 and MTE 3 were 82.4%, 84.7% and 87.1% at 15.8, 13 and 13.1 hours, respectively. In conclusion, the optimal time of predigestion of WS with multi-enzymes (with regard to improvement in its IVDDM) was approximately 14 h.

Relationship between intake of silage and its chemical composition and in vitro digestibility

1972 ◽  
Vol 23 (1) ◽  
pp. 25 ◽  
Author(s):  
DC Brown ◽  
JC Radcliffe
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Ammonia Nitrogen ◽  
Matter Content ◽  
In Vitro Digestibility ◽  
Dry Matter Content ◽  
Regression Analyses ◽  
Lactic Acids

Twenty experimental silages were made from seven pasture species at different stages of maturity. In vivo dry matter, organic matter, and energy ad libitum intakes and digestibilities of the silages were determined with standardized pairs of Merino wethers. The following chemical characteristics of the silages were measured: nitrogen, ammonia nitrogen, total titratable acids, acetic, propionic, butyric, and lactic acids, total volatiles lost during oven drying, lactic acid as a percentage of the total organic acids, pH, acid pepsin dry matter disappearance, dry matter content, and in vitro digestibility and rate of digestion. When all 20 silages were considered, energy intakes on a body weight basis were significantly related to silage pH (r = 0.55) and rate of in vitro digestion (r = 0.58). When the five legume silages were removed from the analysis and only the 15 grass-dominant silages were considered, dry matter intakes were significantly related to acetic (r = –0.57) and propionic acid (r = –0.55) concentrations. Multiple regression analyses did not significantly increase the accuracy of predicting intake. The results suggested that silage intake was negatively related to the degree of fermentation that occurred during the ensiling process.

In vitromicrobial growth and rumen fermentation of different substrates as affected by the addition of disodium malate

2005 ◽  
Vol 81 (1) ◽  
pp. 31-38 ◽  
Author(s):  
M. L. Tejido ◽  
M. J. Ranilla ◽  
R. García-Martínez ◽  
M. D. Carro
Keyword(s):  
Dry Matter ◽  
Microbial Growth ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Mean Values ◽  
Batch Cultures ◽  
Production Values ◽  
Micro Organisms ◽  
Microbial N

AbstractThe effects of two concentrations of disodium malate on thein vitrofermentation of three substrates differing in their forage: concentrate ratio (0·8: 0·2, 0·5: 0·5 and 0·2: 0·8; g/g dry matter; low-, medium- and high-concentrate substrates, respectively) by rumen micro-organisms were studied using batch cultures. Rumen contents were collected from four Merino sheep offered lucerne hay ad libitum and supplemented daily with 400 g concentrate. Disodium malate was added to the incubation bottles to achieve final concentrations of 0, 4 and 8 mmol/l malate and15N was used as a microbial marker. Gas production was measured at regular intervals from 0 to 120 h of incubation to study fermentation kinetics. When gas production values were corrected for gas released from added malate, no effects (P> 0·05) of malate were detected for any of the estimated gas production parameters. In 17-h incubations, the final pH and total volatile fatty acid (VFA) production were increased (P< 0·001) by the addition of malate, but no changes (P> 0·05) were detected in the final amounts of ammonia-N and lactate. When net VFA productions were corrected for the amount of VFA produced from malate fermentation itself, adding malate did not affect (P> 0·05) the production of acetate, propionate and total VFA. Malate reduced methane (CH4) production by proportionately 0·058, 0·013 and 0·054 for the low-, medium- and high-concentrate substrates, respectively. Adding malate to batch cultures increased (P< 0·01) rumen microbial growth (mean values of 16·6, 18·3 and 18·4 mg of microbial N for malate at 0, 4 and 8 mmol/l, respectively), but did not affect (P> 0·05) its efficiency of growth (55·5, 56·7 and 54·3 mg of microbial N per g of organic matter apparently fermented for malate at 0, 4 and 8 mmol/l, respectively). There were no interactions (P> 0·05) malate × substrate for any of the measured variables, and no differences (P> 0·05) in pH, CH4production and microbial growth were found between malate at 4 and 8 mmol/l. The results indicate that malate had a beneficial effect on in vitro rumen fermentation of substrates by increasing VFA production and microbial growth, and that only subtle differences in the effects of malate were observed between substrates. Most of the observed effects, however, seem to be due to fermentation of malate itself.

EFFECT OF TANNINS ON THE DIGESTIBILITY OF TWO ISOSYNTHETIC STRAINS OF BIRDSFOOT TREFOIL (Lotus corniculatus L.) USING IN VITRO AND IN SACCO TECHNIQUES

1988 ◽  
Vol 68 (3) ◽  
pp. 751-760 ◽  
Author(s):  
J. CHIQUETTE ◽  
K.-J. CHENG ◽  
J. W. COSTERTON ◽  
L. P. MILLIGAN
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
In Vitro Digestion ◽  
Lotus Corniculatus ◽  
Birdsfoot Trefoil ◽  
Dry Matter Loss ◽  
Forage Digestibility ◽  
High Concentrations ◽  
In Sacco

Two new isosynthetic strains of birdsfoot trefoil (Lotus corniculatus L.), containing low or high concentrations of tannin, were tested using in vitro and in sacco digestibility techniques. The high tannin (HT) strain contained an average of five times more tannin on a dry matter basis, than the low tannin (LT) strain. After 24-h in vitro digestion, by a rumen inoculum, there was less (P < 0.01) dry matter disappearance (45.7 vs. 54.4%) from the HT strain. Also, the development of gas pressure was lower (P < 0.01) and production of volatile fatty acids less (P < 0.05) with the HT strain than with the LT strain. Average dry matter loss from four replicated nylon bags was also lower (P < 0.01) for the HT strain than for the LT strain after 8, 12, and 24 h of incubation in rumen. These data indicate that a LT strain of birdsfoot trefoil might improve the forage digestibility. Key words: Birdsfoot trefoil, tannin, digestibility

Prediction of in vivo dry matter, organic matter, and energy digestibilities of silage in in vitro digestion techniques

1971 ◽  
Vol 22 (5) ◽  
pp. 787 ◽  
Author(s):  
GD Brown ◽  
JC Radcliffe
Keyword(s):  
Organic Matter ◽  
Volatile Compounds ◽  
Dry Matter ◽  
In Vitro Digestion ◽  
Digestion Time ◽  
Dry Matter Digestibility ◽  
Highly Correlated ◽  
Ad Libitum Intake

Twenty experimental silages were made from seven pasture species at different stages of maturity. In vivo dry matter, organic matter, and energy digestibilities of the silages were determined at restricted and ad libitum intake levels on standardized pairs of Merino wethers. In vitro dry matter digestibility determinations were made on ground frozen silage and ground oven-dried silage with and without a correction for the loss of volatile compounds during drying. Corrected in vitro dry matter digestibilities of ground oven-dried silage for a 48 hr rumen liquor digestion time gave the most accurate prediction of in vivo dry matter (r = 0.88), organic matter (r = 0.86), and energy (r = 0.74) digestibilities for restricted intake levels. In vitro digestibilities of ground frozen silage more accurately predicted in vivo digestibilities than did in vitro digestibilities of ground oven-dried silage without the correction for volatile losses. In vivo dry matter and organic matter digestibilities were highly correlated (v = 0.97), but both were poorly correlated with in vivo energy digestibilities. In vitro digestion of ground oven-dried silage with a correction for the volatile compounds lost during drying is recommended as a suitable routine determination for predicting the in vivo digestibility of silage.

Pattern of intake of three roughage diets by non-pregnant, non-lactating Scottish Blackface ewes over a long period and the effects of previous nutritional history on current intake

1978 ◽  
Vol 26 (2) ◽  
pp. 203-215 ◽  
Author(s):  
Janet Z. Foot ◽  
A. J. F. Russel
Keyword(s):  
Food Intake ◽  
Dry Matter ◽  
Tritiated Water ◽  
Live Weight ◽  
Dry Matter Digestibility ◽  
Negative Effect ◽  
Voluntary Intake ◽  
Positive Effect ◽  
Water Space

ABSTRACTThe voluntary intake of two hays and one dried grass by 48 mature non-pregnant Blackface ewes was measured for 33 weeks. The apparent dry-matter digestibility coefficients of the diets in vitro were 59, 61 and 77% respectively for the hays and the dried grass. At the end of the first phase of the experiment each group of 16 ewes was split into two subgroups of five animals, each of which was subsequently given one of the other diets, and one subgroup of six animals which remained on the same diet. Food intake was measured for a further 8 weeks. Body fat was estimated at intervals from tritiated water space.The amount of variation in voluntary food intake that could be attributed to variation in live weight and fatness of the animals and in apparent dry-matter digestibility and cell wall constituents of the diet was calculated for various periods.The range of live weight and fatness increased during the experiment. Initially, and after the change in diets, variation in apparent dry-matter digestibility had an important positive effect on intake, but at the end of the 33-week phase its influence was negligible or, when fat weight was not considered, negative. The negative effect of fat weight on intake increased during the experiment.Within individual diets during short periods of time (1 to 5 weeks) the highest proportions of the variation in intake that could be attributed to variation in live weight and fatness of the ewes were 50, 94 and 65% respectively for the hays and the dried grass. Taking all three diets together all the variables considered could explain 75% of the variation for a few weeks at a time, but for longer periods seasonal influences, and other factors not included among the independent variables, probably operated.

The effect of chloroform introduced into the rumen of the lactating goat on the yield and composition of the milk

1980 ◽  
Vol 47 (1) ◽  
pp. 51-60
Author(s):  
Saeid Basmaeil ◽  
John L. Clapperton
Keyword(s):  
Food Intake ◽  
Dry Matter ◽  
Apparent Digestibility ◽  
Dietary Energy ◽  
Total Volatile ◽  
Combustible Gas ◽  
Volatile Fatty Acid Concentration ◽  
Lactating Goats ◽  
The Individual

SummaryIn 2 experiments, chloroform was introduced into the rumen of lactating goats given a mixture of 40% chopped hay and 60% barley-based concentrates. The effects on food intake, apparent digestibility, rumen fermentation and the yield and composition of milk were measured. In expts 1 and 2 food intake was reduced by 7% and less than 1% respectively. There were no significant effects on apparent digestibility in expt 1, but there were significant increases in the apparent digestibility of the dry matter and organic matter in expt 2. The results of in vitro determinations suggested that the loss of energy in combustible gas was reduced by 52 and by 25% in expts 1 and 2 respectively. As a result of these changes, the metabolizability of the dietary energy was increased by 2·1 and 4·8% respectively. In both experiments, the total volatile fatty acid concentration in the rumen was reduced when chloroform was added and there were changes in the molar proportions of the individual fatty acids, principally a reduction in acetic acid and an increase in propionic acid. Milk yield was not affected, but the fat content of the milk was reduced and, in expt 1, the protein content was increased.

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