scholarly journals The role of rumen protozoa in the utilization of paspalum (Paspalum dilatatum) hay by cattle

1987 ◽  
Vol 57 (3) ◽  
pp. 395-406 ◽  
Author(s):  
B. S. Punia ◽  
Jane Leibholz ◽  
G. J. Faichney
Keyword(s):  
Retention Time ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Live Weight ◽  
Water Volume ◽  
Paspalum Dilatatum ◽  
Mean Retention Time ◽  
Acid Detergent Fibre ◽  
Dietary Treatments

1.Six Friesian heifers (250 kg live weight) with permanent cannulas in the rumen and abomasum were allocated at random into two groups of three. One group was treated with Teric GN9 (ICI (Aust.) Ltd) to defaunate the animals during the first two of the four periods of the experiment, after which they were refaunated. The second group was treated with Teric at the end of the first two periods. The dietary treatments were: paspalum (Paspalum dilatatum) hay (4.1 kg/d) given alone and the hay supplemented with urea (20 g/kg dry matter).2. Defaunation was not complete but the approximate volume of protozoa in the rumen of treated animals was less than 6% of that in the untreated animals.3. The amount of organic matter (OM) digested in the stomach was lower (P< 0.01) in animals with reduced fauna than in those with normal fauna. There were reductions in both the apparent OM digestibility in the total tract (from 0.56 to 0.52,P< 0.01) and the proportion of the digestible OM digested in the rumen (from 0.82 to 0.79, not significant) of animals with reduced fauna. Apparent digestibilities of acid-detergent fibre and neutral-detergent fibre were significantly lower (P< 0.01) in animals with reduced fauna.4. The amount of nitrogen disappearing from the stomach was significantly higher (P< 0.01) with the urea supplement; effects due to concentrations of protozoa were not significant. The flow of non-ammonia-N from the abomasum was higher (P< 0.05) in animals with reduced fauna than in animals with normal fauna. The flows of bacterial N from the abomasum and the efficiencies of bacterial N synthesis were not significantly affected by the treatments. N retention was higher (P< 0.01) in animals receiving the urea supplement but differences due to protozoa were not significant.5. Protozoal contribution to the microbial N flowing from the rumen of animals with normal fauna was estimated to be 24 and 27% with and without the urea supplement respectively.6. Concentrations of rumen-fluid ammonia-N were reduced (P< 0.05) and those of volatile fatty acids were increased (P< 0.01) with reduction in protozoal numbers. Molar proportions of propionic acid increased (P< 0.05) and of butyric acid decreased (P< 0.01) with reduced rumen fauna.7. Rumen water volume was lower (P< 0.05) and the mean retention time of indigestible acid-detergent lignin tended to be higher in animals with reduced fauna. Rumen dry-matter pool and mean retention time of CrEDTA were not significantly different between treatments.

Effects of urea and saccharose on intake and digestion of a Digitaria decumbens hay by black belly sheep

1999 ◽  
Vol 69 (2) ◽  
pp. 403-410 ◽  
Author(s):  
H. Archimède ◽  
G. Aumont ◽  
G. Saminadin| ◽  
E. Deprès ◽  
P. Despois ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nitrogen Availability ◽  
Latin Square ◽  
Live Weight ◽  
Ruminal Bacteria ◽  
Digitaria Decumbens ◽  
Acid Detergent Fibre ◽  
Kinetics Of

AbstractEffects of incorporation of urea and saccharose in diets, on intake and digestion by sheep of a 35-day-old pangola (Digitaria decumbens) hay, have been studied according to a 4 × 4 Latin-square design. Sixteen rams were given four diets: hay (C); hay plus urea (U, 23 g/kg hay); hay plus saccharose (S, 60 g/kg hay), hay plus urea and saccharose (SU). Acid-detergent fibre and crude protein (CP) content of the roughage were 395 and 78 g/kg dry matter (DM) respectively. Intake of hay (g DM per kg live weight0.75), supplemented with urea and sugar (42⋅9), was lower (F < 0.05) than with other diets (47.2, s.e. 1.6). The organic matter (OM) total tract and ruminal digestibility of the diet C, S, U and SU were 0.622, 0.590, 0.615 and 0.587 (s.e. 0.007); 0.361, 0.380, 0.378 and 0.345 (s.e. 0.015) respectively. Effective degradation and nylon bag kinetics of DM degradation were higher with U and SU compared with S or C. Few differences were observed between diets for ruminal concentration of volatile fatty acids. The ruminal ammonia concentrations were higher (P < 0.05) for the diets with urea than without urea (78 v. 215 mg/l). The efficiencies of the microbial synthesis (g nitrogen per kg OM fermented in the rumen) were, 23.6, 22.4, 24.9 and 29.3 (s.e. 1.7) for the diets C, U, S and SU respectively.Even though additional urea increased nitrogen availability for ruminal bacteria, urea supplementation did not affect significantly intake or digestion of the pangola hay of medium CP content used in this experiment.

A note on the effects of high levels of dietary calcium, phosphorus and sodium on nutrient utilization by sheep offered a roughage-based diet

1991 ◽  
Vol 53 (2) ◽  
pp. 249-252 ◽  
Author(s):  
M. F. J. van Houtert ◽  
R. A. Leng
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Nitrogen Retention ◽  
Live Weight ◽  
Nutrient Utilization ◽  
Wool Growth ◽  
Calcium Phosphorus ◽  
Mineral Concentrations ◽  
Male Sheep

Castrated male sheep were offered a roughage without (control sheep, no. = 8) or with calcium, phosphorus and sodium (CaPNa sheep, no. = 8; Ca, P and Na intake 34, 10 and 17 g/day per sheep respectively, given twice daily as drench). Food intake and live-weight change were unaffected. Water intake and urine excretion were higher whereas dry-matter apparent digestibility, nitrogen retention and wool growth were lower in the CaPNa sheep. The excretion of allantoin in urine indicated no differences in microbial protein absorption from the gut. There were small differences in the proportions of volatile fatty acids, numbers of protozoa and mineral concentrations in rumen fluid between the sheep in the two groups. Retentions of Ca, P and Na were higher in the CaPNa sheep, whereas retention of potassium was lower.

The rate of passage of food through the alimentary tract of pigs

1956 ◽  
Vol 47 (2) ◽  
pp. 196-204 ◽  
Author(s):  
Elizabeth J. Castle ◽  
M. E. Castle
Keyword(s):  
Retention Time ◽  
Dry Matter ◽  
Alimentary Tract ◽  
Live Weight ◽  
Matter Content ◽  
Dry Matter Content ◽  
Mean Retention Time ◽  
A Value ◽  
The Mean ◽  
Fattening Pigs

A method for measuring the rate of passage of meal through the alimentary tract of pigs by feeding a stained marker is described. The results are given of sixty-four experiments made under standardized conditions on four fattening pigs with live weights ranging from 49·9 to 185·5 lb. and six experiments on a sow weighing 500 lb.Results were expressed by means of excretion curves. These were sigmoid in shape and from them a value termed R, the mean retention time (hr.) of all the stained particles in the alimentary tract, directly proportional to the area to the left of the curve, was calculated.In the fattening piga the first appearance of the marker in the faeces occurred 10–24 hr. after feeding, and the mean 5 and 95% excretion times were 21 and 53 hr. respectively. The marker was completely excreted after approximately 80–90 hr. The R value averaged 34·2 hr.A marker fed at the morning feed (9 a.m.) had earlier 5 and 95% excretion times and the R value was significantly smaller.There was no statistically significant relationship between the R values and live weight, digestibility of the dry matter of the ration or the dry-matter content of the faeces.In the sow the 5 and 95% excretion times were 38 and 68 hr. respectively, and the R value averaged 51·1 hr.Counts of stained particles in the intestines of two of the pigs after slaughter showed that food was delayed mainly in the large intestine.

Apparent digestibility, fecal particle size, and mean retention time of reduced lignin alfalfa hay fed to horses

2021 ◽  
Author(s):  
Amanda M Grev ◽  
Marcia R Hathaway ◽  
Craig C Sheaffer ◽  
M Scott Wells ◽  
Amanda S Reiter ◽  
...  
Keyword(s):  
Particle Size ◽  
Retention Time ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
Apparent Digestibility ◽  
Mean Retention Time ◽  
Alfalfa Hay ◽  
Dietary Treatments ◽  
Collection Phase ◽  
Fecal Collection

Abstract Reduced lignin alfalfa (Medicago sativa L.) has the potential to provide a higher quality forage source for livestock by improving forage digestibility. This study was conducted to evaluate apparent digestibility when feeding reduced lignin and non-reduced lignin alfalfa hay to adult horses, and to examine mean fecal particle size (MFPS) and mean retention time (MRT) between alfalfa forage types. In 2017, reduced lignin (‘54HVX41’) and non-reduced lignin (‘WL355.RR’) alfalfa hay was harvested in Minnesota at the late-bud stage. Alfalfa hays were similar in crude protein (CP; 199 g kg -1), neutral detergent fiber (NDF; 433 g kg -1), and digestible energy (DE; 2.4 Mcal kg -1). Acid detergent lignin (ADL) concentrations were lower for reduced lignin alfalfa hay (74 g kg -1) compared to non-reduced lignin alfalfa hay (81 g kg -1). Dietary treatments were fed to six adult, stock-type horses in a crossover study. Experimental periods consisted of a 9-d dietary adaptation phase followed by a 5-d total fecal collection phase, during which horses were housed in individual boxstalls and manure was removed on a continuous 24-h basis. At 12-h intervals, feces were thoroughly mixed, subsampled in duplicate, and used for apparent digestibility and MFPS analysis. On day 2 of the fecal collection phase, horses were fed two indigestible markers, cobalt (Co) and ytterbium (Yb), which were fed as Co-EDTA and Yb-labelled NDF residue, respectively. Additional fecal samples were taken at 2-h intervals following marker dosing until 96-h post-dosing to evaluate digesta MRT. Data was analyzed using the MIXED procedure of SAS, with statistical significance set at P ≤ 0.05. Dietary treatment (i.e., alfalfa hay type) was included as a fixed effect, while experimental period and horse were considered random effects. Dietary treatments were similar in dry matter intake (DMI; 1.6% BW) and time to consumption (TTC; 7.6 h). Apparent dry matter digestibility (DMD) was greater for reduced lignin alfalfa (64.4%) compared to non-reduced lignin alfalfa (61.7%). Apparent CP and NDF digestibility did not differ between dietary treatments, averaging 78% and 45%, respectively. Dietary treatments were similar in MFPS (0.89 mm) and MRT for both liquid (23.7 h) and solid (27.4 h) phase material. These results indicate an improvement in DMD for reduced lignin alfalfa hay when fed to adult horses, with no change in forage consumption, fecal particle size, or digesta retention time.

The effect of silage fermentation characteristics on dry-matter intake of steers

1998 ◽  
Vol 66 (1) ◽  
pp. 105-113 ◽  
Author(s):  
L. E. R. Dawson ◽  
C. S. Mayne
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Eating Behaviour ◽  
Live Weight ◽  
Dietary Treatment ◽  
Experimental Period ◽  
Period Change ◽  
Silage Fermentation ◽  
Dietary Treatments

AbstractJuke was expressed from two silages of contrasting fermentation type with either high (H) or low (L) levels of lactic acid and reconstituted either with the silage from which it had been expressed or the other silage. This procedure produced four dietary treatments (HH, HL, LL, LH) each differing in fermentation characteristics. The dietary treatments HH, HL, LL, and LH contained 81, 71, 59, and 49 g lactic acid per kg dry matter (DM) and 101, 133, 193 and 159 g ammonia-N per kg total nitrogen (TN) respectively. The four diets were offered to four steers (mean live weight 679 (s.e. 49) kg) in a four-period change-over design experiment. Each period was of 17 days duration. DM intakes were recorded daily, with intakes on days 8 to 17 used in the statistical analysis of data. On day 12 of each experimental period, rumen fluid samples were taken throughout a 24-h period. The rates of disappearance of DM, nitrogen (N), modified acid-detergent fibre (MADF) and neutral-detergent fibre (NDF) in the rumen were determined on days 14 to 17 using the polyester bag technique. DM intakes were 7·2, 6·9, 6·0 and 6·0 (s.e. 0·50) kg/day (P = 0·08) for HH, HL, LL and LH dietary treatments respectively. Diet had no significant effect on eating behaviour, rumen fermentation parameters or degradability of DM, N, MADF and NDF fractions (P > 0·05). Silage L increased rumen fluid pH (P < 0·05), reduced buffering capacity (P < 0·05) and increased the molar proportion of propionic acid and n-butyric acid (P < 0·05 and P < 0·01) compared with silage H. Silage L also had a lower DM apparent digestibility, D-value and DM, (P < 0·001), N and MADF (P < 0·05) degradabilities than silage H. The higher DM intake of dietary treatment HH compared with dietary treatments LL and LH could not be explained by any single constituent of the silages although it was apparent that physical characteristics of the silage were more important in determining intake than the chemical characteristics.

Prediction of the voluntary intake of grass silages by beef cattle. 1. Linear regression analyses

1990 ◽  
Vol 50 (3) ◽  
pp. 425-438 ◽  
Author(s):  
A. J. Rook ◽  
M. Gill
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Live Weight ◽  
Ammonia Nitrogen ◽  
Dry Matter Intake ◽  
Digestible Organic Matter ◽  
Voluntary Intake ◽  
Relationship Of ◽  
The Relationship ◽  
Linear Regressions

ABSTRACTData on individually recorded silage dry-matter intake (SDMI), concentrate dry-matter intake (CDMI) and live weight of steers and data on silage composition including toluene dry matter (TDM), pH, total nitrogen (N), ammonia nitrogen (NH3-N), volatile fatty acids (VFAs), digestible organic matter in the dry matter (DOMD) and neutral-detergent fibre (NDF) obtained from experiments conducted at three sites were used to obtain simple and multiple linear regressions of SDMI on other variables.Live weight accounted for a high proportion of the variation in intake but this effect could generally be removed by scaling intake by live weight raised to the power of 0·75 (M0·75). CDMI was the most important factor affecting scaled intake in mixed diets. TDM, NH,-N and VFAs all had important effects on SDMI. The relationship of SDMI with TDM was curvilinear suggesting that there is little to be gained in intake terms from wilting to TDM above 250 g/kg. The effect of NH3-N appeared to be related more to its correlation with VFAs than with any other nitrogenous constituent while the VFAs appeared to have a direct effect on SDMI. The effects of N and pH on SDMI were generally small. DOMD and NDF had relatively little effect on SDMI. Significant differences in intercepts between sites were found for most relationships although common slopes were often found.

scholarly journals Non-structural carbohydrates in the nutrition of high-yielding dairy cows during a transition period

2010 ◽  
Vol 55 (No. 11) ◽  
pp. 468-478 ◽  
Author(s):  
K. Poláková ◽  
V. Kudrna ◽  
A. Kodeš ◽  
B. Hučko ◽  
Z. Mudřík
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Body Condition ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Live Weight ◽  
Dry Matter Intake ◽  
Control Group ◽  
Maize Starch ◽  
Milk Performance

The main aim of this study was to investigate experimentally the effect of different composition of non-structural carbohydrates (NFC) in prepartum feed rations administered to high-yielding dairy cows at a high concentration of NFC in the diet on dry matter intake both before and after parturition and on subsequent milk performance, body condition and physiological traits of rumen fluid and blood. Thirty-six high-yielding dairy cows were allocated into one of the three well-balanced groups (K, O, and C), and each group received a different feeding rations. Feeding rations differed in non-structural carbohydrate (NFC) structure. The "K" (control) group received a feeding ration with NFC in the form of maize starch in particular, while the feeding rations of the other two (experimental) groups contained either (besides maize starch) saccharose from dried sugar beet (the "O" group) or a dominant amount of NFC was in the form of saccharose (the "C" group). After calving, all dairy cows were given the same feeding ration from the first day after parturition. The experiment was conducted for 21 days before and 50 days after calving. FR in the form of total mixed ration was offered ad libitum. Dry matter intake, milk performance, body condition, live weight, and blood and rumen parameters were recorded for the duration of the experiment. Average daily dry matter intake before calving was highest in the "K" group (14.32 kg per head). Differences among groups were statistically significant (P &lt; 0.05). Prepartum dry matter consumption dropped as the rate of saccharose in the diet of cows increased. Dry matter consumption levelled off after calving. Milk yield was also highest in the "K" group (43.71 kg/head/day), but fatness of milk and thus the production of fat corrected milk were lowest in this group. The highest milk fat content (4.10%) and fat corrected milk production (44.03 kg/head/day) were recorded in the "C" group, whereas the highest milk protein concentration was found in the milk of the "O" group. The composition of NFC affected dry matter intake before parturition, but these concentrations did not significantly affect dry matter intake, milk yield, milk composition, live weight, body condition or blood serum and rumen fluid parameters after calving

NUTRITIVE VALUE OF FORMALDEHYDE-TREATED RAPESEED MEAL FOR DAIRY CALVES

1972 ◽  
Vol 52 (2) ◽  
pp. 363-371 ◽  
Author(s):  
H. R. SHARMA ◽  
J. R. INGALLS ◽  
J. A. McKIRDY
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Protein Digestibility ◽  
Rapeseed Meal ◽  
Dairy Calves ◽  
Plasma Urea ◽  
Significant Difference ◽  
Daily Gain

Treatment of oilseed meals with formaldehyde (FA) and glutaraldehyde (GA) significantly (P < 0.01) reduced the solubility of protein in 0.02 N NaOH and the production of NH3 in the artificial rumen. Ten Holstein and Holstein × Brown Swiss calves of both sexes were fed two test rations containing either 26% untreated or formaldehyde-treated (5.6 g FA per 100 g protein) rapeseed meal (FA-treated RSM) for 14 weeks. Plasma urea nitrogen (N) and rumen NH3 levels were significantly (P < 0.01) lower in the FA-treated RSM calves compared with the control calves during the first 8 weeks of the growth trial. No significant difference (P > 0.05) in dry matter consumption, daily gain, or feed efficiency was observed. Total volatile fatty acids (VFA) (mmoles/100 ml) concentration was significantly higher (P < 0.05) in rumen fluid from the control calves compared with those receiving treated RSM. Molar percentages of the VFA’s were not significantly different (P > 0.05) between the two groups. In the N balance and digestibility trial, dry matter consumption did not differ significantly (P > 0.05); however, the FA-treated RSM resulted in decreased (P < 0.05) dry matter and crude protein digestibility. Calves receiving the treated RSM had reduced (P < 0.01) urinary N excretion; however, there was no significant effect (P > 0.05) on N consumption and retention.

Relationship between dry matter, fibre and nitrogen degradation characteristics of silage and silage intake of steers

2000 ◽  
Vol 70 (3) ◽  
pp. 537-546 ◽  
Author(s):  
L.E.R. Dawson ◽  
R.W.J. Street
Keyword(s):  
Dry Matter ◽  
Live Weight ◽  
Ammonia Concentration ◽  
Fibre Concentration ◽  
The Poor ◽  
Acid Detergent Fibre ◽  
Highly Correlated ◽  
The Relationship ◽  
Poor Relationship

AbstractA study was carried out to examine the relationship between silage intake and rumen degradability characteristics of silage. The dry matter (DM), neutral-detergent fibre (NDF), acid-detergent fibre (ADF), hemicellulose and nitrogen (N) degradability characteristics of 136 silages were determined using polyester bags in three rumen fistulated beef cross steers. The bags were incubated for 0, 6, 12, 24, 48 and 72 h. Intakes of these silages, by steers, were reported by Steen et al., 1998. The relationships between silage DM intake (g/kg metabolic live weight) and DM, fibre and N degradability characteristics of the silages were poor (R2 < 0·32). Degradability characteristics of the silages were highly negatively correlated with fibre concentration of the silages (R < –0·82) and highly positively correlated with the soluble N minus ammonia concentration (R < 0·77). Some of the degradability characteristics were highly correlated with organic matter digestibility, particularly the proportion of DM, fibre and N degraded after 72 h (R = 0·75 to 0·85). In view of the poor relationship between silage intake and degradability, the results of this study question the rôle of degradation characteristics of DM, fibre and N in the rumen on silage intake.

ALFALFA SUPPLEMENTATION OF CORNCOB DIETS FOR SHEEP: COMPARISON WITH HIGHER VOLATILE FATTY ACIDS, SOYBEAN PROTEIN AND ALFALFA FIBER ON INTAKE, ENVIRONMENT AND DIGESTION IN THE RUMEN AND DIGESTA PASSAGE

1987 ◽  
Vol 67 (4) ◽  
pp. 1083-1091 ◽  
Author(s):  
L. R. NDLOVU ◽  
J. G. BUCHANAN-SMITH
Keyword(s):  
Fatty Acids ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Soybean Protein ◽  
Control Diet ◽  
Basal Diet ◽  
Latin Squares ◽  
Protein Preparation ◽  
Alfalfa Hay

Nine lambs fitted with rumen cannulae were used in two 5 × 5 Latin squares to measure the effect of alfalfa hay, C4- and C5-branched- and straight-chain volatile fatty acids (C4/C5 VFAs), cell wall from alfalfa hay (ALFCW) and a soybean protein preparation on intake and rumen digestion of a basal corncob diet with urea included at 30 g kg−1. Relative to the control, alfalfa hay decreased (P < 0.05) intake of the basal diet but increased (P < 0.05) total dry matter intake. ALFCW and soybean protein did not affect intake of the basal diet and total dry matter (P > 0.05). C4/C5 VFAs increased intake of basal diet by sheep in one square (P < 0.05) but had no effect (P > 0.05) on intake with sheep in the other square. Alfalfa hay, ALFCW and soybean protein increased (P < 0.05) total VFA concentration in rumen fluid while only alfalfa hay and C4/C5 VFAs consistently increased isobutyrate, isovalerate and valerate levels in rumen fluid (P < 0.05). In comparison with the control diet, soybean protein elevated (P < 0.05) mean rumen ammonia-N levels whereas C4/C5 VFAs depressed them; however, values exceeded 17 mg dL−1 rumen fluid on all treatments. Most measures of digestion kinetics of fiber from corncobs were unaffected by supplementation (P > 0.05). Alfalfa hay and ALFCW increased (P < 0.05) rate of passage of digesta from the reticulo-rumen. It was concluded that alfalfa hay has a reduced role in improving intake of corncob diets when there is also sufficient NPN in the diet. Key words: Roughage (poor quality), volatile fatty acids, rumen ammonia levels, chromium-mordanted cell walls

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