scholarly journals Production leptospirosis vaccine with included strain of Leptospira interrogans of serogroup Canicola

2021 ◽  
Vol 937 (2) ◽  
pp. 022015
Author(s):  
G Urban ◽  
O.Y. Krotova ◽  
K. C. Savenkov ◽  
A Chernyshkov ◽  
M. N. Savenkova
Keyword(s):  
Culture Media ◽  
Epidemiological Data ◽  
Foreign Protein ◽  
Leptospira Interrogans ◽  
Vaccine Production ◽  
Main Method ◽  
Specific Antibodies ◽  
The Russian Federation ◽  
Membrane Technologies ◽  
And Storage

Abstract The leptospirosis vaccine is the main method of preventing the occurrence and spread of leptospirosis. Compliance with the standards of manufacturing, labeling, and storage is mandatory for immunological preparations. All stages of vaccine production must comply with the rules established by the Ministry of Industry and Trade and ensure its safety for humans. The article presents epidemiological data on leptospirosis in the Russian Federation in the period from 2013 to 2018. A method for producing a vaccine against human leptospirosis is described. The leptospirosis vaccine is polyvalent using membrane technologies and semi-synthetic culture media. It eliminates the use of foreign protein and does not require cleaning. The vaccine is an opalescent liquid with sediment and a pH of 7.2-7.6 and it is not allowed to contain live leptospira. Four strains are used and a new strain has been developed and implemented. Vaccination is carried out according to epidemiological indicators. Leptospirosis suspension forms specific immunity for 1 year. During the production of the updated vaccine, it was necessary to study the virulent properties of the strains. Moreover, analyze the formation of specific antibodies to leptospira in the new vaccine and in the vaccine currently used. From 2018 to 2020, 5 series of experimental vaccines in the form of a 0.5 ml suspension were produced.

ANALYTICAL EPIDEMIOLOGY OF GASTRIC CANCER IN THE REPUBLIC OF CRIMEA AND ITS CONFORMITY WITH ALL-RUSSIAN TRENDS

2018 ◽  
Vol 64 (5) ◽  
pp. 592-601
Author(s):  
Viktor Oleksenko ◽  
Kazim Aliev ◽  
I. Akinshevich ◽  
Ye. Chirva
Keyword(s):  
Gastric Cancer ◽  
Epidemiological Data ◽  
Mortality Rates ◽  
Similar Data ◽  
Results Of Treatment ◽  
Mortality Index ◽  
World Standard ◽  
The Russian Federation ◽  
The Republic ◽  
National Trends

Gastric cancer (GC) is one of the most common malignant tumor, both world-wide and in the Russian Federation (RF), possessing one of the highest mortality rates. The aim of current research was to analyze the main epidemiological data, the rates reflecting the diagnostics and results of treatment of GC patients in the Republic of Crimea (RC) and to compare with national trends. Using the extensive, intensive, standardized rates, estimated by world standard method, structural analysis of the epidemiology of GC in RC for the period from 2007 to 2016 was carried out. The obtained results made it possible to compare these data with the main GC rates in RF. Results of the study. The incidence of GC in RC decreased during 10 years, for men - 16,42 (4th place), for women - 6,68 (9th place) per 100 000 of the population. By 2021 a further decline in morbidity in men is expected to be 30,27% and a possible increase in the female incidence rate - by 17,54%. The average age of GC patients in RC was 66,5 years. Index accuracy was 0,75, which testified to satisfactory conditions of specialized treatment for this tumor. Mortality from GC at the 1st year of life in RC was higher than in RF - 56,0%, which was due to low active diagnostics - 3,6% and accordingly a high proportion of GC patients of IV stage - 43,3%. Ratio index in RC for 10 years was higher than in RF and increased from 3,5 to 4,4; prevalence rate of GC in RC was lower - 84,1 per 100 000 of the population in comparison with RF, GC mortality index - 15,3 per 100 000 of the population that was lower than in RF. The proportion of patients who have been observed for 5 years or more in RC was 57,3% that was more than in RF. Conclusions. The rates reflecting early diagnostics of GC in RC are worse than all-Russian ones, which makes it necessary to develop medical examination program for the population of RC for this malignancy. The growth of ratio index, the lower mortality rates and the greater proportion of people who have been observed for 5 years or more indicates the best results of treatment of patients with GC in RC compared with similar data in RF.

Reactive Arthritis: Modern Aspects of Diagnosis and Treatment

2020 ◽  
Vol 65 (7-8) ◽  
pp. 63-70
Author(s):  
B. S. Belov ◽  
R. M. Balabanova
Keyword(s):  
Russian Federation ◽  
Clinical Picture ◽  
Reactive Arthritis ◽  
Epidemiological Data ◽  
Controlled Trials ◽  
Antimicrobial Drugs ◽  
Randomized Controlled ◽  
Laboratory Techniques ◽  
High Prevalence ◽  
The Russian Federation

Currently, the problem of reactive arthritis (ReA) retains its importance due to the fairly high prevalence of the disease, primarily in Russia. Analysis of epidemiological data allows us to put forward a number of possible reasons explaining the different frequency of ReA in certain regions of the Russian Federation and in other countries. The lecture describes the clinical picture of the disease, as well as analyzes the significance of various laboratory techniques aimed at identifying the causative agent of ReA. The Russian diagnostic criteria for ReA are presented. The main approaches to the therapy of ReA are outlined with an emphasis on the use of antimicrobial drugs. The effectiveness and safety of drug immunocorrection (inducers of interferon, polyoxidonium, immunofan, etc.) in the treatment of urogenital chlamydia in patients with ReA have not been confirmed by data from randomized controlled trials.

Fluorescent antibody techniques have allowed for the direct identification and enumeration of individual bacteria in environmental samples without requiring prior growth in culture media (Bahlool and Schmidt 1980, Cloete and Steyn 1988, Macario et al. 1989). The technique involves the use of specific antibodies raised against surface markers of defined pure cultures that are either labelled directly with fluorescent dye molecules or via a fluorescent secondary antibody. This approach has yielded important insights into the spatial distribution of microorganisms, but it suffers from a number of disadvantages. For example, expression of the antigen may be influenced by environmental factors; false-positive and false-negative results may be obtained due to cross-reactivity or lack of reaction; non-specific binding of antibodies may result in high levels of background fluorescence; and production of specific antibodies requires a pure culture of the organism of interest (Cloete and de Bruyn Various recombinant DNA techniques have subsequently been developed that are independent of cultivation methods (Fig. 1). These techniques provide ways of detecting and quantifying specific phylogenetic groups of microbes on 16S rDNA sequences, and relevant structural genes provide ways of monitoring microbial populations of environmental and industrial systems. In addition to these tools, a number of emerging technologies such as the use of biomarker genes are being increasingly used to monitor with great precision and accuracy the behaviour of microbes in the environment.

2003 ◽  
pp. 218-219
Keyword(s):  
Recombinant Dna ◽  
Culture Media ◽  
Specific Binding ◽  
Fluorescent Antibody ◽  
False Negative ◽  
Cross Reactivity ◽  
Phylogenetic Groups ◽  
Specific Antibodies ◽  
Negative Results ◽  
Recombinant Dna Techniques

scholarly journals Human babesiosis: clinical cases in the european part of the Russian Federation

2019 ◽  
Vol 91 (11) ◽  
pp. 60-65
Author(s):  
A K Tokmalaev ◽  
V B Chentsov ◽  
V A Malov ◽  
V V Maleyev ◽  
G M Kozhevnikova ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Russian Federation ◽  
Clinical Picture ◽  
Epidemiological Data ◽  
European Part ◽  
Laboratory Studies ◽  
Direct Supervision ◽  
Clinical Observations ◽  
The Russian Federation ◽  
Human Babesiosis

The article describes four clinical observations of patients with babesiosis detected in the European part of the Russian Federation, two of whom were under the direct supervision of the authors. The analysis of epidemiological data, clinical picture, results of laboratory studies in the dynamics of the disease. Differential diagnosis and treatment are discussed.

Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review

2021 ◽  
Vol 95 ◽  
Author(s):  
B. Bobić ◽  
V. Ćirković ◽  
I. Klun ◽  
T. Štajner ◽  
J. Srbljanović ◽  
...  
Keyword(s):  
Systematic Review ◽  
Russian Federation ◽  
Grey Literature ◽  
Taenia Solium ◽  
Epidemiological Data ◽  
Intermediate Hosts ◽  
Time Dynamic ◽  
The Status ◽  
Search Data ◽  
The Russian Federation

Abstract Taenia solium is a zoonotic parasite that causes taeniasis and cysticercosis in humans (as final hosts) and cysticercosis in pigs (as intermediate hosts). The Russian Federation (RF) is traditionally considered as endemic for this zoonosis. However, the epidemiological data on T. solium infection have not been reviewed for the past 20 years, in which time dynamic economical and societal changes have occurred in the RF. The aim of this systematic review was to analyse the status of T. solium infection in RF in the 2000–2019 period. A literature search was conducted, which collected published articles, grey literature and official data on the epidemiology of T. solium taeniasis and cysticercosis in the RF published from 2000. From a total of 2021 articles and 24 official reports originally returned by the search, data were extracted from 12 full text articles and 11 official reports. Taenia solium taeniasis was continuously reported in the RF between 2000 and 2019, with a tenfold decrease in the incidence, from 0.2 per 100,000 population in 2000 to 0.023/100,000 in 2019. Also, the number of administrative units where taeniasis was detected continuously decreased. Cysticercosis in pigs had a declining trend after 2006. In conclusion, although decreasing, T. solium infection is still endemic in several regions and suspected to be endemic in most of the RF.

scholarly journals The Correlation of Educational Standards for Museology (Ba)

2019 ◽  
Vol 8 (7) ◽  
pp. 34
Author(s):  
Olga A. Masalova ◽  
Makka I. Dolakova ◽  
Marina A Mefodeva ◽  
Adelya Sattarova
Keyword(s):  
Higher Education ◽  
Comparative Analysis ◽  
Education System ◽  
Radical Change ◽  
Educational Process ◽  
Professional Standards ◽  
Educational Standards ◽  
Main Method ◽  
Higher Education System ◽  
The Russian Federation

This article is devoted to the problem of correlation of the higher education system of the Russian Federation in accordance with the requirements of professional standards. The relevance of this problem is due to a radical change in approaches to personnel Museum policy and in the profile system of higher education. The most controversial issue in this study is the fragmentary implementation of professional standards in the field of Museum business and the dependence of the educational process on them. The article reveals the problems of determining the qualification requirements for a number of Museum professions, and assessing the possibility of their solution at the level of the education system. The main method of research is the method of comparative analysis, which allowed to determine the content load of the definitions used and to correlate professional and educational standards in the study area. The materials of the article can be useful for the formation of working curricula in the field of museology and protection of cultural and natural heritage.

Digitalization of Russian Economy within the Framework of the National Innovation Policy

2019 ◽  
Vol 10 (4) ◽  
pp. 1274
Author(s):  
Marsel M. IMAMOV ◽  
Natalia B. SEMENIKHINA
Keyword(s):  
Russian Federation ◽  
Innovation Policy ◽  
Legal Regulation ◽  
State Regulation ◽  
Analysis Method ◽  
Main Method ◽  
Legal Documents ◽  
Russian Economy ◽  
Regulatory Aspect ◽  
The Russian Federation

National innovation policy is part of socio-economic policy and is focused on improving state regulation, development and stimulation of innovation. It is implemented using economic, regulatory and other mechanisms of state support. Digital economy affects the growth of the country's economy and the measurement of gross domestic product, productivity and sustainability of economic entities in all financial spheres. The purpose of the article is to analyze the national innovation policy in the regulatory aspect, as well as to analyze the state of development of science and innovation in the Russian Federation. In the process of research, the main method employed was a comparative analysis method, which allowed to achieve the purpose by means of a comprehensive research of the identified issue. To achieve the purpose, the following objectives were set: to consider the legal regulation of the innovation field in the Russian Federation; to evaluate the implemented and existing programs for the development of science and innovation in the Russian Federation. A review of legal documents allowed to conclude that they are of high importance for the development of the innovation field, since they constitute a systemic basis for the implementation of modernization processes and the qualitative improvement of the country's socio-economic development.

SCREW-CAPPED BOTTLES IN THE PREPARATION AND STORAGE OF CULTURE MEDIA

The Lancet ◽  
1933 ◽  
Vol 222 (5738) ◽  
pp. 433-436 ◽  
Author(s):  
J.E. Mccartney
Keyword(s):  
Culture Media ◽  
And Storage

Use of whey permeate containing in situ synthesised galacto-oligosaccharides for the growth and preservation of Lactobacillus plantarum

2013 ◽  
Vol 80 (3) ◽  
pp. 374-381 ◽  
Author(s):  
Marina Golowczyc ◽  
Carlos Vera ◽  
Mauricio Santos ◽  
Cecilia Guerrero ◽  
Paula Carasi ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Culture Media ◽  
Enzymatic Reaction ◽  
Raw Material ◽  
Whey Permeate ◽  
Cheese Industry ◽  
Potential Impact ◽  
And Storage ◽  
Kinetics Of

Galacto-oligosaccharides (GOS) are prebiotics that have a beneficial effect on human health by promoting the growth of probiotic bacteria in the gut. GOS are commonly produced from lactose in an enzymatic reaction catalysed by β-galactosidase, named transglycosylation. Lactose is the main constituent of whey permeate (WP), normally wasted output from the cheese industry. Therefore, the main goal of this work was to optimise the synthesis of GOS in WP using β-galatosidase from Aspergillus oryzaea. WP and whey permeate enzymatically treated (WP-GOS) were used as culture media of Lactobacillus plantarum 299v. Lb. plantarum 299v attained the stationary phase in approximately 16 h, reaching 3·6 and 4·1×108 CFU/ml in WP and WP-GOS, respectively. The in situ synthesised GOS were not consumed during growth. No significant differences were observed in the growth kinetics of microorganisms in both media. After fermentation, microorganisms were dehydrated by freeze-drying and spray-drying and stored. The recovery of microorganisms after fermentation, dehydration and storage at 4 °C for at least 120 d was above 108 CFU/g. These studies demonstrated that WP is an appropriate substrate for the synthesis of GOS and the obtained product is also adequate as culture medium of Lb. plantarum 299v. The coexistence of GOS and dehydrated viable probiotic microorganisms, prepared using an effluent as raw material, represents the main achievement of this work, with potential impact in the development of functional foods.

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