Effect of Thai Silk Fibroin on Mechanical Properties of Bioactive Glass Silk Fibroin Hybrid Bone Scaffolds

Collagen, gelatin, silk fibroin, hyaluronic acid, chitosan, alginate, and cellulose are biocompatible and non-cytotoxic, being attractive natural polymers for medical devices for both soft and hard tissues. However, such natural polymers have low bioactivity and poor mechanical properties, which limit their applications. To tackle these drawbacks, collagen, gelatin, silk fibroin, hyaluronic acid, chitosan, alginate, and cellulose can be combined with bioactive glass (BG) nanoparticles and microparticles to produce composites. The incorporation of BGs improves the mechanical properties of the final system as well as its bioactivity and regenerative potential. Indeed, several studies have demonstrated that polymer/BG composites may improve angiogenesis, neo-vascularization, cells adhesion, and proliferation. This review presents the state of the art and future perspectives of collagen, gelatin, silk fibroin, hyaluronic acid, chitosan, alginate, and cellulose matrices combined with BG particles to develop composites such as scaffolds, injectable fillers, membranes, hydrogels, and coatings. Emphasis is devoted to the biological potentialities of these hybrid systems, which look rather promising toward a wide spectrum of applications.

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Fabrication of hierarchically porous silk fibroin-bioactive glass composite scaffold via indirect 3D printing: Effect of particle size on physico-mechanical properties and in vitro cellular behavior

Materials Science and Engineering C ◽

10.1016/j.msec.2019.04.067 ◽

2019 ◽

Vol 103 ◽

pp. 109688 ◽

Cited By ~ 9

Author(s):

Mina Razaghzadeh Bidgoli ◽

Iran Alemzadeh ◽

Elnaz Tamjid ◽

Mona Khafaji ◽

Manouchehr Vossoughi

Keyword(s):

Mechanical Properties ◽

Particle Size ◽

3D Printing ◽

Bioactive Glass ◽

Silk Fibroin ◽

Composite Scaffold ◽

Glass Composite ◽

Cellular Behavior ◽

Effect Of Particle Size

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Osteogenic enhancement of silk fibroin-based bone scaffolds by forming hybrid composites with bioactive glass through GPTMS during sol-gel process

Materials Today Communications ◽

10.1016/j.mtcomm.2020.101730 ◽

2020 ◽

pp. 101730

Author(s):

Juthatip Manissorn ◽

Pimnara Wattanachai ◽

Khaow Tonsomboon ◽

Palang Bumroongsakulsawat ◽

Siriporn Damrongsakkul ◽

...

Keyword(s):

Bioactive Glass ◽

Silk Fibroin ◽

Hybrid Composites ◽

Sol Gel ◽

Bone Scaffolds ◽

Sol Gel Process

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Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels

Biomolecules ◽

10.3390/biom10101456 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1456

Author(s):

Isabel Matos Oliveira ◽

Cristiana Gonçalves ◽

Myeong Eun Shin ◽

Sumi Lee ◽

Rui Luis Reis ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Mechanical Properties ◽

Silk Fibroin ◽

Therapeutic Efficacy ◽

Composite Structures ◽

Gelation Time ◽

Polymeric Materials ◽

Gellan Gum ◽

Traditional Use

Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist. Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine. However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation. Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties. In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty–GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA). The Ty–GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2–5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time. In vitro studies showed that Ty–GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation. Furthermore, Ty–GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone. Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.

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Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

International Journal of Molecular Sciences ◽

10.3390/ijms22073391 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3391

Author(s):

Sylwia Grabska-Zielińska ◽

Alina Sionkowska ◽

Ewa Olewnik-Kruszkowska ◽

Katarzyna Reczyńska ◽

Elżbieta Pamuła

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Physicochemical Properties ◽

Silk Fibroin ◽

Three Dimensional ◽

Microscopy Imaging ◽

Maximum Deformation ◽

One Step ◽

Chitosan Blends ◽

Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

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Novel Hydrogels of Chitosan and Poly (vinyl alcohol) Reinforced with Inorganic Particles of Bioactive Glass

Polymers ◽

10.3390/polym13050691 ◽

2021 ◽

Vol 13 (5) ◽

pp. 691

Author(s):

O. Sánchez-Aguinagalde ◽

Ainhoa Lejardi ◽

Emilio Meaurio ◽

Rebeca Hernández ◽

Carmen Mijangos ◽

...

Keyword(s):

Mechanical Properties ◽

Drug Release ◽

Bioactive Glass ◽

Vinyl Alcohol ◽

Release Kinetics ◽

The Body ◽

Poly Vinyl Alcohol ◽

X Ray Diffraction ◽

Inorganic Particles ◽

Release Studies

Chitosan (CS) and poly (vinyl alcohol) (PVA) hydrogels, a polymeric system that shows a broad potential in biomedical applications, were developed. Despite the advantages they present, their mechanical properties are insufficient to support the loads that appear on the body. Thus, it was proposed to reinforce these gels with inorganic glass particles (BG) in order to improve mechanical properties and bioactivity and to see how this reinforcement affects levofloxacin drug release kinetics. Scanning electron microscopy (SEM), X-ray diffraction (XRD), swelling tests, rheology and drug release studies characterized the resulting hydrogels. The experimental results verified the bioactivity of these gels, showed an improvement of the mechanical properties and proved that the added bioactive glass does affect the release kinetics.

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Investigation of the biological activity, mechanical properties and wound healing application of a novel scaffold based on lignin–agarose hydrogel and silk fibroin embedded zinc chromite nanoparticles

RSC Advances ◽

10.1039/d1ra01300a ◽

2021 ◽

Vol 11 (29) ◽

pp. 17914-17923

Author(s):

Reza Eivazzadeh-Keihan ◽

Hooman Aghamirza Moghim Aliabadi ◽

Fateme Radinekiyan ◽

Mohammad Sobhani ◽

Farzane khalili ◽

...

Keyword(s):

Mechanical Properties ◽

Wound Healing ◽

Biological Activity ◽

Silk Fibroin ◽

Agarose Hydrogel ◽

Novel Scaffold ◽

Fibroin Solution ◽

Zinc Chromite

Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin–agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr2O4) nanoparticles.

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Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties

Carbohydrate Polymers ◽

10.1016/j.carbpol.2020.116802 ◽

2020 ◽

Vol 248 ◽

pp. 116802 ◽

Cited By ~ 1

Author(s):

Reza Eivazzadeh-Keihan ◽

Fateme Radinekiyan ◽

Hamid Madanchi ◽

Hooman Aghamirza Moghim Aliabadi ◽

Ali Maleki

Keyword(s):

Mechanical Properties ◽

Graphene Oxide ◽

Silk Fibroin ◽

Blood Compatibility

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Additive manufacturing of PLA-based scaffolds intended for bone regeneration and strategies to improve their biological properties

e-Polymers ◽

10.1515/epoly-2020-0046 ◽

2020 ◽

Vol 20 (1) ◽

pp. 571-599

Author(s):

Ricardo Donate ◽

Mario Monzón ◽

María Elena Alemán-Domínguez

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Additive Manufacturing ◽

Bone Regeneration ◽

Polylactic Acid ◽

State Of The Art ◽

Biological Properties ◽

Design Stage ◽

Bone Scaffolds ◽

Regeneration Of Bone

AbstractPolylactic acid (PLA) is one of the most commonly used materials in the biomedical sector because of its processability, mechanical properties and biocompatibility. Among the different techniques that are feasible to process this biomaterial, additive manufacturing (AM) has gained attention recently, as it provides the possibility of tuning the design of the structures. This flexibility in the design stage allows the customization of the parts in order to optimize their use in the tissue engineering field. In the recent years, the application of PLA for the manufacture of bone scaffolds has been especially relevant, since numerous studies have proven the potential of this biomaterial for bone regeneration. This review contains a description of the specific requirements in the regeneration of bone and how the state of the art have tried to address them with different strategies to develop PLA-based scaffolds by AM techniques and with improved biofunctionality.

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