Favorable Crisscrossing Pattern With Polydioxanone: Barbed Thread Lifting in Constructing Fibrous Architecture

2021 ◽  
Author(s):  
Jennifer Kim Song ◽  
Jiyeon Chang ◽  
Kae Won Cho ◽  
Yong Choi Chang
Keyword(s):  
Paradigm Shift ◽  
Control Group ◽  
Dorsal Skin ◽  
Nude Mouse Model ◽  
Hematoxylin And Eosin ◽  
Tissue Specimens ◽  
Experimental Group ◽  
Extension Effect ◽  
Pattern Width ◽  
Sirius Red

Abstract Background The longevity of polydioxanone (PDO)-barbed lifting threads remains controversial. Objectives To assess the longevity extension effect of a crisscross implantation pattern in PDO-barbed thread lifting. Methods To acquire the desired outcome in PDO-barbed thread lifting, we suggested a paradigm shift to incorporate biochemical factors in enforcing the physico-mechanical lift. A nude mouse model was used to evaluate our theory, i.e., to compare the conventional fan-shaped protocols in barbed thread lifting with an architectural construction of intersections of fibrous capsule in a crisscross pattern. Three fragments of monofilament PDO-barbed lifting threads were implanted in the dorsal skin of 12 nude mice. The pattern of implantation was fan-shaped in the control group and crisscross in the experimental group. Tissue specimens containing tangential areas of threads were harvested, fixed, and paraffin-embedded. Samples were horizontally cut and histologically analyzed using hematoxylin and eosin, Massons’ Trichrome, and Sirius red staining. Fibrotic areas and the width of fibrosis from the thread were also analyzed. Results Fibrous capsulations around the barbed area of the PDO-barbed lifting threads were three-fold greater than those around the barb-free areas of the threads. In the crisscross implantation pattern, width and density of the fibrotic areas were five-fold greater than those of the fan-shaped areas. Induction of fibrous capsules around the PDO-barbed thread was markedly condensed in the crisscross areas. Conclusions This study provides the basis for more logical implantation pattern in PDO-barbed lifting threads for facial rejuvenation. By generating controlled multiple crisscross patterns, we can create more intense fibrogenesis, reduce tension applied on each barbed threads and as a result, extend the longevity of the result.

scholarly journals Research in the precaution of recombinant human erythropoietin to steroid-induced osteonecrosis of the rat femoral head

2017 ◽  
Vol 45 (4) ◽  
pp. 1324-1331 ◽  
Author(s):  
Lu-yong Jiang ◽  
Xiao Yu ◽  
Qing-jiang Pang
Keyword(s):  
Femoral Head ◽  
Recombinant Human Erythropoietin ◽  
Positive Control ◽  
The Other ◽  
Positive Control Group ◽  
Control Group ◽  
Human Erythropoietin ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
Experimental Group

Objective To elucidate the effects of recombinant human erythropoietin (rHuEPO) on steroid-induced osteonecrosis of the femoral head in rats. Methods Twenty-four adult Wistar rats were randomly divided into three groups of eight rats each. The rats in the positive control group were injected with dexamethasone at 1 mg/kg twice a week for 5 weeks. The rats in the negative control group were injected with sodium chloride alone. The rats in the experimental group were injected with dexamethasone at 1 mg/kg twice a week for 5 weeks and rHuEPO (500 u/d/kg) daily for 5 weeks. The femoral head on one side was examined by hematoxylin and eosin staining, and that on the other side was examined by CD31 staining of the capillaries. Results Hematoxylin and eosin staining in the positive control group showed that the bony trabeculae had become obviously narrow and sparse with discontinuity of the integrity. The integrity of the trabeculae was better in the experimental group than positive control group. The CD31 expression was lower in the positive control group than in the other two groups. Conclusion rHuEPO can effectively prevent osteocyte apoptosis, delaying or decreasing osteonecrosis of the femoral head.

scholarly journals Effect of Radiation Therapy on Wound Healing

2019 ◽  
Author(s):  
Gashtasb Aghasinejad ◽  
Mehdi Raei ◽  
Saeid Azad ◽  
Bahareh Aghasinejad
Keyword(s):  
Wound Healing ◽  
Light Exposure ◽  
Skin Incision ◽  
Control Group ◽  
Visible Radiation ◽  
Accelerate Wound Healing ◽  
Radiation Control ◽  
Hematoxylin And Eosin ◽  
And Control ◽  
Experimental Group

Abstract- Many studies were carried out to improve sophisticated approaches to accelerate wound healing processes and also, wound healing is extensively discussed in the medicinal literature. In this study, we aimed to investigate the wound healing of mice with visible radiation (630 nm). In this study, 28 male mice were used and randomly divided into 4 groups. All samples were subjected to a 6 mm skin incision. Four groups were tested as following 10 minutes (First group), 20 minutes (Second group), 30 minutes (Third group) light exposure and no light radiation (control group), respectively. Wound contraction was analyzed by taking photos and Image J software. All mice were sacrificed, and samples were evaluated by Hematoxylin and Eosin staining after one week. The results demonstrated that the experimental group with 20 min.light irradiation had a much faster effect of wound healing than the 10, 30 minute and control group. The results showed that 20 minutes of light exposure group was the best treatment.

scholarly journals Morphometric justification of osteosynthesis using implants coated with titanium and hafnium nitrides

2016 ◽  
Vol 97 (4) ◽  
pp. 585-591
Author(s):  
D E Tsyplakov ◽  
A E Izosimova ◽  
F V Shakirova ◽  
I F Akhtyamov ◽  
E B Gatina
Keyword(s):  
Stainless Steel ◽  
Soft Tissues ◽  
Medullary Canal ◽  
Control Group ◽  
Tibial Osteotomy ◽  
Bone Fragments ◽  
Hematoxylin And Eosin ◽  
12X18h9t Steel ◽  
Incomplete Closure ◽  
Experimental Group

Aim. To perform comparative and morphological assessment of the reparative processes in the bone and the surrounding soft tissues during intramedullary osteosynthesis using stainless steel implants and titanium nitride coated stainless steel implants.Methods. 40 rabbits who underwent open tibial osteotomy followed by retrograde insertion of the implant into the medullary canal served as an experimental model. 2 mm diameter nails of 12X18H9T steel coated with titanium and hafnium nitrides were used. In the control group, similar nails without coating were used. Histological sections of bone fragments and postoperative wounds, which were taken for morphological study on the 10th, 30th, 60th and 180th day from the beginning of the experiment, were stained with hematoxylin and eosin and with picrofuchsin by Van-Gieson method.Results.The percentage of leukocyte-necrotic masses on the 10th day was 3.5±0.2% in the experimental group and 6.0±0.4% in the comparison group (pConclusion. Osteosynthesis using nails coated with titanium and hafnium nitrides contributes to reduction of the inflammatory response in the surrounding tissues, accelerates the process of reparative regeneration, providing an earlier wound epithelialization and reducing the likelihood of complications development such as incomplete closure of bone defect.

scholarly journals Analysis of Development Mechanism of Giant Cell Arteritis in Nude Mouse Model through Color Duplex Sonography and Computerized Tomography Nanocontrast Agent

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Fugang Chen ◽  
Yang Li ◽  
Hongyan Zhou ◽  
Chuang Sun ◽  
Sun Li ◽  
...  
Keyword(s):  
Mouse Model ◽  
Nude Mouse ◽  
Nude Mice ◽  
Temporal Artery ◽  
Duplex Sonography ◽  
The Body ◽  
Control Group ◽  
Color Duplex Sonography ◽  
Nude Mouse Model ◽  
Experimental Group

To explore the application value of color duplex sonography and enhanced computerized tomography (CT) inspection based on a nanocontrast agent in diagnosis and pathogenesis in giant cell arteritis (GCA), the GCA nude mouse model was constructed. In this study, 40 healthy male BalB/c nude mice aged 6-8 weeks were randomly divided into a control group (no model) and an experimental group (model), with 20 mice in each group, and the temporal artery tissue of GCA patients diagnosed as positive by temporal artery biopsy was implanted into nude mice to construct a GCA nude mouse model. Abdominal aortic biopsy and immunohistochemistry were used to verify the success of the GCA nude mouse model. All nude mice were subjected to color duplex sonography and enhanced CT examination based on a nanocontrast agent. At the same time, the basic indicators such as body weight, temperature, white blood cell (WBC), lymphocytes (LYM), hemoglobin (HGB), and platelet (PLT) were measured, and the protein expression levels of interleukin-6 (IL-6) and signal transducer and activator of transcription 3 (STAT3) were detected by immunohistochemistry. The results showed that the temporal artery wall of the nude mice in the experimental group thickened and the lumen was significantly narrowed, indicating that the cell arteritis model of nude mice was successfully constructed; ultrasound examination showed that the right superficial temporal artery vascular cavity narrowed, the blood flow signal changed like a filling defect around the periphery, and there was a low echo halo. CT examination showed that the left superficial temporal artery narrowed, and the inner diameter of the narrow segment of blood vessels changed like a bead. The body weight of nude mice in the experimental group decreased significantly after the modeling was completed ( P < 0.05 ); after modeling, the body temperature of the nude mice in the experimental group increased significantly ( P < 0.05 ); LYM and HGB values of nude mice in the experimental group were significantly lower than those in the control group ( P < 0.05 ); the content of IL-6, STAT3, IL-6, and STAT3 proteins in the arterial tissue of nude mice in the experimental group was lower than that of the control group ( P < 0.05 ), indicating that color duplex sonography and CT contrast agent technology can be used in the diagnosis and development mechanism research of GC.

scholarly journals Interleukin-6 (IL-6) influence on colonic anastomosis healing in rats

2007 ◽  
Vol 22 (2) ◽  
pp. 110-114 ◽  
Author(s):  
Fernando Hintz Greca ◽  
Zacarias Alves de Souza Filho ◽  
Allan Giovanini ◽  
Carlos Agemiro de Camargo Junior ◽  
Michel Risnic Rubin ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Colonic Anastomosis ◽  
Collagen Type I ◽  
Histological Evaluation ◽  
Control Group ◽  
Type I ◽  
Colonic Healing ◽  
Red Dye ◽  
Experimental Group ◽  
Sirius Red

PURPOSE:To observe the effect of pro-inflamatory citokin, Interleukin-6 (IL-6), administration on the healing of colonic anastomosis in rats. METHODS: Thirty Wistar rats were divided into 2 groups of 15 animals each: a control group and an experiment group. The animals of the experiment group were submitted to injection of 500µg/kg of IL-6 Recombinant (Sandoz Phamarceutical®) subcutaneously 24 and 12 hours preceding the surgery, and the control group, were submitted to the injection of saline solution, using the same volume of solution as well as the way of administration. The rats were submitted to an end to end colonic anastomosis, being sacrificed in the 7th post-operative day, the site of the anastomosis was evaluated regarding to the adhesions that was quantified and the presence of complications was observed. The colon containing the anastomosis was submitted to a tensiometric study and a sample were sent for histological studies with HE and Sirius Red dye. RESULTS: The macroscopic and the tensiometric study did not demonstrate any significant differences between the control and experiment group. Regarding the histological evaluation, there was a significant reduction of the chronic inflammatory process in the experiment group (p=0,0209) and a lesser granulation area (p=0,0099). The total amount of collagen was similar in both groups, however, the experimental group showed a predominant area of immature collagen (p=0.009). CONCLUSIONS: The longer persistence of the acute phase of wound healing in the experiment group, as well as a decreased of the mature collagen type I, demonstrating that the IL-6 was harmful to the colonic healing anastomosis in rats.

scholarly journals Effects of Zeaxanthin on Growth and Invasion of Human Uveal Melanoma in Nude Mouse Model

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaoliang L. Xu ◽  
Dan-Ning Hu ◽  
Codrin Iacob ◽  
Adrienne Jordan ◽  
Sandipkumar Gandhi ◽  
...  
Keyword(s):  
Uveal Melanoma ◽  
Nude Mice ◽  
Treated Group ◽  
Control Group ◽  
Histopathological Analysis ◽  
Nude Mouse Model ◽  
Promising Agent ◽  
Quantitative Image ◽  
Hematoxylin And Eosin ◽  
Mitotic Figures

Uveal melanoma cells were inoculated into the choroid of nude mice and treated with or without intraocular injection of zeaxanthin. After 21 days, mice were sacrificed and the eyes enucleated. Histopathological analysis was performed in hematoxylin and eosin stained frozen sections. Melanoma developed rapidly in the control group (without treatment of zeaxanthin). Tumor-bearing eye mass and tumor mass in the control group were significantly greater than those in zeaxanthin treated group. Melanoma in the controlled eyes occupied a large part of the eye, was epithelioid in morphology, and was with numerous mitotic figures. Scleral perforation and extraocular extension were observed in half of the eyes. Melanomas in zeaxanthin treated eyes were significantly smaller with many necrosis and apoptosis areas and no extraocular extension could be found. Quantitative image analysis revealed that the tumor size was reduced by 56% in eyes treated with low dosages of zeaxanthin and 92% in eyes treatment with high dosages of zeaxanthin, as compared to the controls. This study demonstrated that zeaxanthin significantly inhibits the growth and invasion of human uveal melanoma in nude mice, suggesting that zeaxanthin may be a promising agent to be explored for the prevention and treatment of uveal melanoma.

scholarly journals Challenge Tooth Regeneration in Adult Dogs with Dental Pulp Stem Cells on 3D-Printed Hydroxyapatite/Polylactic Acid Scaffolds

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3277
Author(s):  
Rung-Shu Chen ◽  
Sheng-Hao Hsu ◽  
Hao-Hueng Chang ◽  
Min-Huey Chen
Keyword(s):  
Stem Cells ◽  
Polylactic Acid ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Control Group ◽  
Tooth Regeneration ◽  
3D Printed ◽  
Hematoxylin And Eosin ◽  
The Right ◽  
Experimental Group

Tooth regeneration is an important issue. The purpose of this study was to explore the feasibility of using adult dental pulp stem cells on polylactic acid scaffolds for tooth regeneration. Three teeth were extracted from each side of the lower jaws of two adult dogs. In the experimental group, dental pulp stem cells were isolated and seeded in the 3D-printed hydroxyapatite/polylactic acid (HA/PLA) scaffolds for transplantation into left lower jaw of each dog. The right-side jaw of each dog was transplanted with cell-free scaffolds as the control group. Polychrome sequentially labeling was performed for observation of mineralization. Dental cone beam computed tomography (CBCT) irradiation was used for assessment. Nine months after surgery, dogs were euthanized, and the lower jaws of dogs were sectioned and fixed for histological observation with hematoxylin and eosin staining. The results showed that the degree of mineralization in the experimental group with cells seeded in the scaffolds was significantly higher than that of the control group transplanted with cell-free scaffolds. However, the HA/PLA scaffolds were not completely absorbed in both groups. It is concluded that dental pulp stem cells are important for the mineralization of tooth regeneration. A more rapid absorbable material was required for scaffold design for tooth regeneration.

Morphometric analysis of peroxisomes in hepatocytes of alcohol-fed rats

1989 ◽  
Vol 47 ◽  
pp. 1100-1101
Author(s):  
D.C. Dominguez ◽  
J.T. Ellzey
Keyword(s):  
Caloric Intake ◽  
Volume Density ◽  
Controlled Study ◽  
Control Group ◽  
Numerical Density ◽  
Cacodylate Buffer ◽  
Paired Control ◽  
Experimental Group ◽  
Liver Peroxisomes ◽  
Single Lesion

Peroxisomes which participate in 1ipid metabolism have been shown to be altered in several metabolic disorders and toxic conditions. In alcoholic liver disease, the single lesion most frequently found is lipid accumu1ation in hepatocytes. However, the mechanisms for this 1ipid accumu1ation are not clear. The occurrence of modifications of liver peroxisomes due to excess alcohol consumption has not been subjected to a controlled study. We utilized a combination of cytochemica1 and morphometrictechniques to study the size and number of liver peroxisomes in rats fed an alcohol-supplemented diet compared to those of matched-paired control animals.Male Sprague-Daw1ey rats (400-500 g) received a liquid diet. The experimental group (N = 5/group) was fed a diet containing 30% ethanol-derived calories (EDC) and the control group was fed an isocaloric diet to 30% EDC. A pair feeding procedure was employed to control for caloric intake. Small pieces of liver randomly selected, were fixed in 2.3% -glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.2, incubated in a DAB medium and postfixed with. 2% aqueous osmium tetroxide. EM photographs were taken from sections of 3 tissue blocks from each sample (7,200X) with a Zeiss EM10-A (60 kV). With the use of a point counting method and a digital planimeter the volume density (Vv) and numerical density (Nv) were determined.

Values and intergroup perceptions of East Germans and West Germans in 1998 1This research was supported by a grant from the German Research Society (We 1106/3-1).

1999 ◽  
Vol 58 (4) ◽  
pp. 263-272
Author(s):  
Jörg Doll ◽  
Michael Dick
Keyword(s):  
Research Society ◽  
Frame Of Reference ◽  
Control Group ◽  
Perceived Similarity ◽  
The West ◽  
Need For Uniqueness ◽  
German Research ◽  
East German ◽  
Experimental Group ◽  
East West

The studies reported here focus on similarities and dissimilarities between the terminal value hierarchies ( Rokeach, 1973 ) ascribed to different groups ( Schwartz & Struch, 1990 ). In Study 1, n = 65 East Germans and n = 110 West Germans mutually assess the respective ingroup and outgroup. In this intra-German comparison the West Germans, with a mean intraindividual correlation of rho = 0.609, perceive a significantly greater East-West similarity between the group-related value hierarchies than the East Germans, with a mean rho = 0.400. Study 2 gives East German subjects either a Swiss (n = 58) or Polish (n = 59) frame of reference in the comparison between the categories German and East German. Whereas the Swiss frame of reference should arouse a need for uniqueness, the Polish frame of reference should arouse a need for similarity. In accordance with expectations, the Swiss frame of reference significantly reduces the correlative similarity between German and East German from a mean rho = 0.703 in a control group (n = 59) to a mean rho = 0.518 in the experimental group. Contrary to expectations, the Polish frame of reference does not lead to an increase in perceived similarity (mean rho = 0.712).

Rorschach Characteristics in Adolescents with Systemic Lupus Erythematosus

2006 ◽  
Vol 28 (1) ◽  
pp. 100-118 ◽  
Author(s):  
J. Santoantonio ◽  
L. Yazigi ◽  
E. I. Sato
Keyword(s):  
Lupus Erythematosus ◽  
Case Control Study ◽  
Activity Index ◽  
Disease Activity Index ◽  
Female Adolescents ◽  
Control Group ◽  
Intelligence Scale ◽  
The Mean ◽  
Experimental Group ◽  
Control Study

The purpose of this study was to investigate the personality characteristics in adolescents with SLE. The research design is a case-control study by means of the Rorschach Method and the Wechsler Intelligence Scale. Study group: 30 female adolescents with lupus, 12–17 years of age. The SLE Disease Activity Index was administered during the period of psychological evaluation. Control group: 32 nonpatient adolescents were matched for age, sex, and socioeconomic level. In the Wechsler Intelligence Scale the mean IQ of the experimental group was significantly lower than that of the control group (77 and 98, respectively, p < .001). In the Rorschach, the lupus patients showed greater difficulty in interpersonal interactions, although they displayed the resources to process affect and to cope with stressful situations. A positive moderate correlation (p = .069) between the activity index of the disease and the affect constriction proportion of the Rorschach was observed: the higher the SLEDAI score, the lower the capacity to process affect. There is a negative correlation between the activity index of the disease and the IQ (p = .001): with a higher activity index of the disease, less intellectual resources are available.

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