Lycium barbarum Extracts Extend Lifespan and Alleviate Proteotoxicity in Caenorhabditis elegans

Lycium barbarum berry (Ningxia Gouqi, Fructus lycii, goji berry, or wolfberry), as a traditional Chinese herb, was recorded beneficial for longevity in traditional Chinese medical scriptures and currently is a natural dietary supplement worldwide. However, under modern experimental conditions, the longevity effect of L. barbarum berry and the underlying mechanisms have been less studied. Here, we reported that total water extracts of L. barbarum berry (LBE), which contains 22% polysaccharides and other components, such as anthocyanins, extended the lifespan of Caenorhabditis elegans without side effects on worm fertility and pharyngeal pumping. Interestingly, we found that the lifespan extension effect was more prominent in worms with shorter mean lifespan as compared to those with longer mean lifespan. Furthermore, we showed that the lifespan extension effect of LBE depended on deacetylase sir-2.1. Remarkably, LBE rescued heat shock transcription factor-1 (hsf-1) deficiency in wild-type worms with different mean lifespans, and this effect also depended on sir-2.1. In addition, we found that LBE extended lifespan and alleviated toxic protein aggregation in neurodegenerative worms with hsf-1 deficiency. Our study suggested that LBE may be a potential antiaging natural dietary supplement especially to individuals with malnutrition or chronic diseases and a potential therapeutic agent for neurodegenerative diseases characterized by hsf-1 deficiency.

The Influence of Taiwan Electronic Production Company's Proprietary Data Products on the Interaction of Company Effectiveness

Purpose: The purpose of this study is to explore the unique interaction of companies in Taiwan from 1998 to 2017 and the influence of the degree of international industry on the effectiveness of the company. Approach/Methodology/Design: We used time series methods to investigate and research. This study only has a significant impact on the upstream industry of the electronics industry, and there is no significant development in the middle and lower reaches of the industry. This is a very strange situation in Taiwan. Findings: At the same time, the interaction of the three attributes of the midstream computer industry can also produce the multiplication effect. In this study, it has been found that the degree of international internationalization has a higher impact on the effectiveness of companies, but no matter how high it is, it seems that increasing the degree of international internationalization at about 10% or 50% and more than 80% of the international level can produce and increase the effectiveness of the company. Practical Implications: This is a very strange situation in Taiwan. However, because the research strength has the effect of prolonging the effect, it may not play a significant role in the middle and lower reaches of the research and development of the product extension effect, so that the research intensity does not play a significant role in the first year of the development of the Taiwan electronics industry. Originality/value: Our value is mainly to solve the problems of internationalization and performance of Taiwanese electronics industry companies.

Favorable Crisscrossing Pattern With Polydioxanone: Barbed Thread Lifting in Constructing Fibrous Architecture

Background The longevity of polydioxanone (PDO)-barbed lifting threads remains controversial. Objectives To assess the longevity extension effect of a crisscross implantation pattern in PDO-barbed thread lifting. Methods To acquire the desired outcome in PDO-barbed thread lifting, we suggested a paradigm shift to incorporate biochemical factors in enforcing the physico-mechanical lift. A nude mouse model was used to evaluate our theory, i.e., to compare the conventional fan-shaped protocols in barbed thread lifting with an architectural construction of intersections of fibrous capsule in a crisscross pattern. Three fragments of monofilament PDO-barbed lifting threads were implanted in the dorsal skin of 12 nude mice. The pattern of implantation was fan-shaped in the control group and crisscross in the experimental group. Tissue specimens containing tangential areas of threads were harvested, fixed, and paraffin-embedded. Samples were horizontally cut and histologically analyzed using hematoxylin and eosin, Massons’ Trichrome, and Sirius red staining. Fibrotic areas and the width of fibrosis from the thread were also analyzed. Results Fibrous capsulations around the barbed area of the PDO-barbed lifting threads were three-fold greater than those around the barb-free areas of the threads. In the crisscross implantation pattern, width and density of the fibrotic areas were five-fold greater than those of the fan-shaped areas. Induction of fibrous capsules around the PDO-barbed thread was markedly condensed in the crisscross areas. Conclusions This study provides the basis for more logical implantation pattern in PDO-barbed lifting threads for facial rejuvenation. By generating controlled multiple crisscross patterns, we can create more intense fibrogenesis, reduce tension applied on each barbed threads and as a result, extend the longevity of the result.

The Effect of Mianserin on Lifespan of Caenorhabditis elegans is Abolished by Glucose

Background: The antidepressant mianserin has been shown to extend the lifespan of Caenorhabditis elegans (C. elegans), a well-established model organism used in aging research. The extension of lifespan in C. elegans was shown to be dependent on increased expression of the scaffolding protein (ANK3/unc-44). In contrast, antidepressant use in humans is associated with an increased risk of death. The C. elegans in the laboratory are fed Escherichia coli (E. coli), a diet high in protein and low in carbohydrate, whereas a typical human diet is high in carbohydrates. We hypothesized that dietary carbohydrates might mitigate the lifespan-extension effect of mianserin. Objective: To investigate the effect of glucose added to the diet of C. elegans on the lifespan-extension effect of mianserin. Methods: Wild-type Bristol N2 and ANK3/unc-44 inactivating mutants were cultured on agar plates containing nematode growth medium and fed E. coli. Treatment groups included (C) control, (M50) 50 μM mianserin, (G) 73 mM glucose, and (M50G) 50 μM mianserin and 73 mM glucose. Lifespan was determined by monitoring the worms until they died. Statistical analysis was performed using the Kaplan-Meier version of the log-rank test. Results: Mianserin treatment resulted in a 12% increase in lifespan (P<0.05) of wild-type Bristol N2 worms but reduced lifespan by 6% in ANK3/unc-44 mutants, consistent with previous research. The addition of glucose to the diet reduced the lifespan of both strains of worms and abolished the lifespan-extension by mianserin. Conclusion: The addition of glucose to the diet of C. elegans abolishes the lifespan-extension effects of mianserin.

Ergothioneine exhibits longevity-extension effect in Drosophila Melanogaster via regulating of cholinergic neurotransmission, tyrosine metabolism, and fatty acid oxidation

Many studies have demonstrated the protective effect of ergothioneine (EGT), the unique sulfur-containing antioxidant in mushrooms, on several aging-related diseases. Nevertheless, to date, no single study has explored the potential...