Heparin pretreatment suppresses norepinephrine concentrations in dogs in endotoxic shock.

1977 ◽  
Vol 23 (7) ◽  
pp. 1346-1347 ◽  
Author(s):  
D F Devereux ◽  
C A Michas ◽  
S Rice
Keyword(s):  
Body Weight ◽  
Intravenous Administration ◽  
Endotoxic Shock ◽  
Isotonic Saline ◽  
Control Group ◽  
E Coli ◽  
Physiological Variables ◽  
Blood Pressures

Abstract Mongrel dogs were treated intravenously with either 1000 units of beef-lung heparin per kilogram of body weight or with isotonic saline, before intravenous administration of E. coli endotoxin. We found significant differences in circulating norepinephrine concentrations between a heparin-pretreatment group (1.89 +/- 0.39 microgram/liter) and the control group (9.83 +/- 4.64 microgram/liter), but none with respect to epinephrine. Systolic blood pressures at 360 min were also significantly (P less than 0.05) different, 148 +/- 6 mmHg as compared with 118 +/- 13.4 mmHg. Evidently heparin pretreatment can decrease circulating norepinephrine concentrations in the endotoxic state and changes in circulating catecholamine concentrations can affect physiological variables.

scholarly journals Effect of the Addition of Magnesium Salt to a Feed Mixture on Intestinal Microflora, Health, and Production of Sows

2013 ◽  
Vol 57 (1) ◽  
pp. 69-72 ◽  
Author(s):  
Beata Trawńska ◽  
Antoni Polonis ◽  
Jerzy Lechowski ◽  
Leszek Tymczyna ◽  
Ryszard Borowski ◽  
...  
Keyword(s):  
Body Weight ◽  
Survival Rate ◽  
Reproductive Performance ◽  
Intestinal Microflora ◽  
Alimentary Tract ◽  
Magnesium Salt ◽  
Control Group ◽  
Feed Mixture ◽  
E Coli ◽  
Newborn Piglets

Abstract The effect of feed mixture supplementation with MgCl2 on alimentary tract microflora, haematological markers, and selected parameters of reproductive performance of sows was investigated. The animals were divided into control group (K) and two experimental groups (E1 and E2). The sows from the experimental groups were receiving 1 g of MgCl2 6H20/100 kg b.w./day, which constituted 120 mg of pure magnesium, administered in a small portion of a feed mixture. The sows from group E1 were receiving MgCl2 every day for 30 d, whereas sows from group E2 for 60 d. A significant decrease in the count of E. coli, Providencia sp., and Proteus sp. was noted in faeces of the swine of both experimental groups. The study has also demonstrated a reduced number of lymphocytes and an increased number of granulocytes in blood of the sows receiving MgCl2. Feeding of pregnant sows with the addition of magnesium salt decreased the mortality rate of newborn piglets, increased the survival rate of piglets until 21 d of their life, and increased body weight values of litters originating from experimental sows, compared to the litters of control sows.

scholarly journals Protective role of sildenafil citrate in isoniazid-rifampicin induced histomorphological changes in liver of albino mice

2021 ◽  
Vol 15 (1) ◽  
pp. 52-58
Author(s):  
Najma Hameed ◽  
Khalid Farooq
Keyword(s):  
Body Weight ◽  
Morphological Changes ◽  
Isotonic Saline ◽  
Daily Basis ◽  
Protective Role ◽  
Sildenafil Citrate ◽  
Control Group ◽  
Histomorphological Changes ◽  
Mice Liver

Objectives: The objective of the study was to reveal the reversal of histo-morphological changes in mice liver induced by combined isoniazid-rifampicin (INH-RIF) therapy with sildenafil treatment. Methods: Twenty-one mice weighing between 25–35 g were enrolled in the study. Randomisation was carried out by simple balloting method. The selected mice were sorted into three groups with 7 mice, each group. In group C (n=7) control group, mice were administered 0.4ml of saline per kg body weight daily intra peritoneally for 21 days. In group R (n=7) INH-RIF group, rifampicin (50 mg/kg) and isoniazid (50 mg/kg), dissolved in 4 ml/kg isotonic saline, were administered intra-peritoneally (ip) daily for 21 days. In group S (n=7) sildenafil administered group, 10 mg/kg sildenafil was given orally by gastric gavage on daily basis along with the intraperitoneal injection of INH-RIF (50 mg/kg each) daily for 21 days. Results: Histopathology revealed hepatotoxicity in group R (INH-RIF), while significant improvement was observed in group C (INH-RIF-sildenafil). Conclusion: Sildenafil citrate possesses hepatoprotective role against INH-RIF induced hepatotoxicity.

Role of hypotension in altering blood coagulability after endotoxin

1962 ◽  
Vol 202 (3) ◽  
pp. 519-522 ◽  
Author(s):  
Frank A. Carozza ◽  
J. Dixon Hills
Keyword(s):  
Isotonic Saline ◽  
Treated Group ◽  
Base Line ◽  
E Coli ◽  
Arterial Blood ◽  
Blood Pressures ◽  
Blood Coagulability ◽  
The Mean ◽  
Specific Reactions

Because hypotension, regardless of etiology, profoundly influences blood coagulation, the role of hypotension in altering blood coagulability after endotoxin was investigated. By means of femoral artery catheterization, serial whole blood clotting times were determined in siliconized tubes at 37 C and correlated with mean arterial blood pressures in 1-kg albino rabbits. After duplicate base-line determinations, 200 µg/kg E. coli endotoxin was injected intravenously into nine animals; this quantity of endotoxin was the largest dose not lethal to normal rabbits of the strain employed. Nine control animals received isotonic saline. Blood from the endotoxin-treated group exhibited some accelerated coagulability between 1–2 hr after endotoxin injection and became significantly hypercoagulable ( P < 0.05) during the 3rd and 4th hr. The observed hypercoagulability could not be correlated with the hypotensive arterial blood pressure levels, the mean coefficient of correlation being –0.18. These data suggest that endotoxin-induced hypercoagulability in the rabbit results from specific reactions that are mediated by mechanisms distinct from those known to operate during the hypotensive state per se.

scholarly journals Study of some Impact Of Enterococcus faecium As Probiotic On Chick 1- Body Weight And Pathological Changes In Air Sacs And Intestine

2007 ◽  
Vol 31 (1) ◽  
pp. 70-77
Author(s):  
Emad J. Khamas
Keyword(s):  
Body Weight ◽  
High Temperature ◽  
Enterococcus Faecium ◽  
Control Group ◽  
Pathological Changes ◽  
Fourth Group ◽  
E Coli ◽  
The Third ◽  
Air Sacs ◽  
The Impact

To investigate the impact of Enteococcus faecium as probiotic in layer chicks,this bacteria was given in feed at a dose rate of 1.5 gm per kg feed, each kg offeed contained 5×1011 cfu along the period of the study (57 days). 150 one dayold males were divided into five groups, 30 males each. The 1st group was giventhe probiotic only in the feed, the 2nd group was given the probiotic and exposedto high temperature and bad ventilation as a stress, and the third group wasgiven the probiotic with chlortetracycline in feed while the fourth group wasgiven the probiotic and then infected with E. coli on 21 day of age and the fifthgroup was given feed with out probiotic as the control group. The resultsshowed that there was increase in body weight especially in the last weeks of theexperiment. Turbidity of the air sacs was mild in the four group compared withcontrol. Intestinal villi became elongated due to using of probiotic and there wasless sloughing compared with control, and those groups showed resistancetoward E. coli infection.

Different effects of endotoxic shock on the respiratory function of liver and heart mitochondria in rats

2006 ◽  
Vol 290 (3) ◽  
pp. G543-G549 ◽  
Author(s):  
Andrey V. Kozlov ◽  
Katrin Staniek ◽  
Susanne Haindl ◽  
Christina Piskernik ◽  
Wolfgang Öhlinger ◽  
...  
Keyword(s):  
Temporal Relationship ◽  
Respiratory Activity ◽  
Endotoxic Shock ◽  
Isotonic Saline ◽  
Heart Mitochondria ◽  
Saline Control ◽  
Rat Liver Mitochondria ◽  
Control Group ◽  
Ros Generation ◽  
Ros Production

This study was designed to clarify whether mitochondrial function/dysfunction and reactive oxygen species (ROS) production have a temporal relationship with organ failure during endotoxic shock. Adult male Sprague-Dawley rats were divided into three groups receiving 1) isotonic saline (control group, n = 16); 2) 8 mg/kg lipopolysaccharide (LPS; n = 8); or 3) 20 mg/kg LPS ( n = 8) intraperitoneally under short anesthesia with 3.5% of isoflurane. After 16 h, animals were killed to analyze plasma, rat liver mitochondria (RLM), and rat heart mitochondria (RHM). In accordance with plasma analysis, LPS-treated rats were divided into “responders” and “nonresponders” with high and low levels of alanine aminotransferase and creatine, respectively. RHM from responders had significantly lower respiratory activity in state 3, suggesting a decreased rate of ATP synthesis. In contrast, RLM from responders had significantly higher respiratory activity in state 3 than both nonresponders and the control group. This increase was accompanied by a decrease in phosphate-to-oxygen ratio values, which was not observed in RHM. ROS generation determined with a spin probe, 1-hydroxy-3-carboxypyrrolidine, neither revealed a difference in RHM between LPS and control groups nor between responders and nonresponders. In contrast, RLM isolated from responders showed a marked increase in ROS production compared with both the control group and nonresponders. Our data demonstrate that 1) RHM and RLM respond to endotoxic shock in a different manner, decreasing and increasing respiratory activity, respectively, and 2) there is a temporal relationship between ROS production in RLM (but not in RHM) and tissue damage in rats subjected to LPS shock.

scholarly journals Effects of short term fasting on the evolution of fecal peritonitis in mice

2011 ◽  
Vol 26 (3) ◽  
pp. 181-185 ◽  
Author(s):  
Fernando Antônio Martins Bermudes ◽  
Juliano Bertollo Dettoni ◽  
Fausto Edmundo Lima Pereira
Keyword(s):  
Body Weight ◽  
Endotoxic Shock ◽  
Control Group ◽  
Fecal Peritonitis ◽  
Nacl Solution ◽  
Short Term ◽  
Commercial Diet ◽  
Fecal Suspension ◽  
Ad Libitum ◽  
Resistance To Infection

PURPOSE: To investigate the effect of 72 hours food suppression on the evolution of fecal peritonitis in mice evaluating the mortality and measuring the number and size of abscesses formed into the peritoneal cavity. METHODS: Mice receiving commercial diet and water ad libitum (control group, N=35) and mice fasted during 72 h (N=35), receiving only water ad libitum, were inoculated by i.p. route, with 4uL/g body weight of a fecal suspension diluted 1:6 or 1:9 in 0.15M NaCl solution (1:6 dilution, 22 controls and 18 fasted; 1:9 dilution, 13 controls and 17 fasted). Animals were followed up until two weeks after fecal inoculation, when the survivors were euthanized for evaluation of the number and size of intra-peritoneal abscesses. Mortality was evaluated by Kaplan Meyer curves. RESULTS: Mortality was significantly higher in fasted groups than in controls. However the number and size of abscesses were significantly less in fasted groups than in controls. CONCLUSION: Seventy two hours food suppression increased the susceptibility to endotoxic shock (high mortality after peritonitis induction) and the resistance to infection with fecal microorganisms (less number and size of intra-peritoneal abscesses).

L-Ascorbic Acid Addition to Chitosan Reduces Body Weight in Overweight Women

2014 ◽  
Vol 84 (1-2) ◽  
pp. 5-11 ◽  
Author(s):  
Eun Y. Jung ◽  
Sung C. Jun ◽  
Un J. Chang ◽  
Hyung J. Suh
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Fat Body ◽  
Body Weight Gain ◽  
Skinfold Thickness ◽  
The Body ◽  
Control Group ◽  
Percentage Body Fat ◽  
Overweight Women ◽  
Body Weights

Previously, we have found that the addition of L-ascorbic acid to chitosan enhanced the reduction in body weight gain in guinea pigs fed a high-fat diet. We hypothesized that the addition of L-ascorbic acid to chitosan would accelerate the reduction of body weight in humans, similar to the animal model. Overweight subjects administered chitosan with or without L-ascorbic acid for 8 weeks, were assigned to three groups: Control group (N = 26, placebo, vehicle only), Chito group (N = 27, 3 g/day chitosan), and Chito-vita group (N = 27, 3 g/day chitosan plus 2 g/day L-ascorbic acid). The body weights and body mass index (BMI) of the Chito and Chito-vita groups decreased significantly (p < 0.05) compared to the Control group. The BMI of the Chito-vita group decreased significantly compared to the Chito group (Chito: -1.0 kg/m2 vs. Chito-vita: -1.6 kg/m2, p < 0.05). The results showed that the chitosan enhanced reduction of body weight and BMI was accentuated by the addition of L-ascorbic acid. The fat mass, percentage body fat, body circumference, and skinfold thickness in the Chito and Chito-vita groups decreased more than the Control group; however, these parameters were not significantly different between the three groups. Chitosan combined with L-ascorbic acid may be useful for controlling body weight.

Tryptophan-Niacin Metabolism in Rat with Puromycin Aminonucleoside-Induced Nephrosis

2006 ◽  
Vol 76 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Yukari Egashira ◽  
Shin Nagaki ◽  
Hiroo Sanada
Keyword(s):  
Body Weight ◽  
Urinary Excretion ◽  
Enzyme Activities ◽  
Treated Group ◽  
Control Group ◽  
Puromycin Aminonucleoside ◽  
Low Level ◽  
Key Enzyme

We investigated the change of tryptophan-niacin metabolism in rats with puromycin aminonucleoside PAN-induced nephrosis, the mechanisms responsible for their change of urinary excretion of nicotinamide and its metabolites, and the role of the kidney in tryptophan-niacin conversion. PAN-treated rats were intraperitoneally injected once with a 1.0% (w/v) solution of PAN at a dose of 100 mg/kg body weight. The collection of 24-hour urine was conducted 8 days after PAN injection. Daily urinary excretion of nicotinamide and its metabolites, liver and blood NAD, and key enzyme activities of tryptophan-niacin metabolism were determined. In PAN-treated rats, the sum of urinary excretion of nicotinamide and its metabolites was significantly lower compared with controls. The kidneyα-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD) activity in the PAN-treated group was significantly decreased by 50%, compared with the control group. Although kidney ACMSD activity was reduced, the conversion of tryptophan to niacin tended to be lower in the PAN-treated rats. A decrease in urinary excretion of niacin and the conversion of tryptophan to niacin in nephrotic rats may contribute to a low level of blood tryptophan. The role of kidney ACMSD activity may be minimal concerning tryptophan-niacin conversion under this experimental condition.

Systemic Thrombin Generation and Activity Resistant to Low Molecular Weight Heparin Administered Prior to Streptokinase in Patients with Acute Myocardial Infarction

1997 ◽  
Vol 77 (01) ◽  
pp. 057-061 ◽  
Author(s):  
Dennis W T Nilsen ◽  
Lasse Gøransson ◽  
Alf-Inge Larsen ◽  
Øyvind Hetland ◽  
Peter Kierulf
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Molecular Weight ◽  
Body Weight ◽  
Low Molecular Weight Heparin ◽  
Troponin T ◽  
Bleeding Complications ◽  
Control Group ◽  
Low Molecular Weight ◽  
Creatine Kinase Mb

SummaryOne hundred patients were included in a randomized open trial to assess the systemic factor Xa (FXa) and thrombin inhibitory effect as well as the safety profile of low molecular weight heparin (LMWH) given subcutaneously in conjunction with streptokinase (SK) in patients with acute myocardial infarction (MI). The treatment was initiated prior to SK, followed by repeated injections every 12 h for 7 days, using a dose of 150 anti-Xa units per kg body weight. The control group received unfractionated heparin (UFH) 12,500 IU subcutaneously every 12 h for 7 days, initiated 4 h after start of SK infusion. All patients received acetylsalicylic acid (ASA) initiated prior to SK.Serial blood samples were collected prior to and during the first 24 h after initiation of SK infusion for determination of prothrombin fragment 1+2 (Fl+2), thrombin-antithrombin III (TAT) complexes, fibrinopeptide A (FPA) and cardiac enzymes. Bleeding complications and adverse events were carefully accounted for.Infarct characteristics, as judged by creatine kinase MB isoenzyme (CK-MB) and cardiac troponin T (cTnT), were similar in both groups of patients.A comparable transient increase in Fl+2, TAT and FPA was noted irrespective of heparin regimen. Increased anti-Xa activity in patients given LMWH prior to thrombolytic treatment had no impact on indices of systemic thrombin activation.The incidence of major bleedings was significantly higher in patients receiving LMWH as compared to patients receiving UFH. However, the occurrence of bleedings was modified after reduction of the initial LMWH dose to 100 anti-Xa units per kg body weight.In conclusion, systemic FXa- and thrombin activity following SK-infusion in patients with acute MI was uninfluenced by conjunctive LMWH treatment.

Overtraining of aerobic physical exercise reduce rats (Rattus norvegicus) memory

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Ni Made Ridla Parwata
Keyword(s):  
Body Weight ◽  
Physical Exercise ◽  
Rattus Norvegicus ◽  
Research Method ◽  
Male Rat ◽  
Control Group ◽  
Adult Male Rat ◽  
The Subject ◽  
Heavy Training

Overtraining syndrome is a decrease in physical capacity, emotions and immunity due to training that is too often without adequate periods of rest. Overtraining is often experienced by athletes who daily undergo heavy training with short break periods. This research aims to look at the effect of overtraining aerobic physical exercise on memory in mice. The research method was experimental in vivo with the subject of adult male rat (Rattus Norvegicus) Winstar strain aged 8-10 weeks, body weight 200-250 gr. Divided into three groups, namely the control group, aerobic group and overtraining group. The results of memory tests with water E Maze showed an increase in the duration of travel time and the number of animal errors made by the overtraining group (p = 0.003). This study concludes that overtraining aerobic physical exercise can reduce memory in rat hippocampus.

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