PSXI-8 Heritability estimates of antibody- and cell-mediated immune response in north American angus beef cattle

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 244-245
Author(s):  
Shannon Beard ◽  
Doug C Hodgins ◽  
Julie Schmied ◽  
Jeff Caswell ◽  
Michael M Lohuis ◽  
...  
Keyword(s):  
Immune Response ◽  
Beef Cattle ◽  
North American ◽  
Animal Health ◽  
Serum Antibody ◽  
Intradermal Injection ◽  
Breeding Value ◽  
Breeding Programs ◽  
Heritability Estimates

Abstract Including immune response traits in breeding programs has been suggested as a solution to improve overall animal health and enhance disease resistance but is not yet available for Angus cattle in North America. One way to measure immune response is to use the High Immune Response (HIR™) technology, which identifies animals with superior heritable immunity by evaluating antibody-mediated (AMIR) and cell-mediated (CMIR) immune response, allowing for the calculation of an estimated breeding value for total adaptive immune response for each animal. Before HIR™ can be included in beef cattle breeding programs, genetic parameters, including heritability, must be estimated for the traits involved. The objective of this study was to estimate a preliminary heritability for AMIR and CMIR in North American Angus beef cattle. On day 0 of the phenotyping protocol, cattle were immunized intramuscularly with a preparation of type 1 (CMIR) and type 2 (AMIR) antigens with adjuvant. On day 14, cattle received an intradermal injection of 0.1 mL of the CMIR test antigen in the right tail fold, and 0.1 mL PBS in the left tail fold. Change in cutaneous double skinfold thickness after 24 hours was used to assess CMIR. Blood was collected on days 0 and 14 to evaluate serum antibody to the type 2 antigen by ELISA to quantify AMIR. Heritability estimates were calculated for Canada and the USA separately, and then for both countries combined using single-trait animal models in ASReml and are presented in Table 1. The results of this study indicate that the heritabilities of AMIR and CMIR are moderate and emphasize the potential for its inclusion into Angus breeding schemes.

scholarly journals Dairy Heifers Naturally Exposed toFasciola hepaticaDevelop a Type 2 Immune Response and Concomitant Suppression of Leukocyte Proliferation

2017 ◽  
Vol 86 (1) ◽  
Author(s):  
John Graham-Brown ◽  
Catherine Hartley ◽  
Helen Clough ◽  
Aras Kadioglu ◽  
Matthew Baylis ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Peripheral Blood ◽  
Serum Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mixed Effect ◽  
Content Type ◽  
Grazing Season ◽  
Type 2 Immune Response

ABSTRACTFasciola hepaticais a parasitic trematode of global importance in livestock. Control strategies reliant on anthelmintics are unsustainable due to the emergence of drug resistance. Vaccines are under development, but efficacies are variable. Evidence from experimental infection suggests that vaccine efficacy may be affected by parasite-induced immunomodulation. Little is known about the immune response toF. hepaticafollowing natural exposure. Hence, we analyzed the immune responses over time in calves naturally exposed toF. hepaticainfection. Cohorts of replacement dairy heifer calves (n= 42) with no prior exposure toF. hepatica, on three commercial dairy farms, were sampled over the course of a grazing season. Exposure was determined through anF. hepatica-specific serum antibody enzyme-linked immunosorbent assay (ELISA) and fluke egg counts. Concurrent changes in peripheral blood leukocyte subpopulations, lymphocyte proliferation, and cytokine responses were measured. Relationships between fluke infection and immune responses were analyzed by using multivariable linear mixed-effect models. All calves from one farm showed evidence of exposure, while cohorts from the remaining two farms remained negative over the grazing season. A type 2 immune response was associated with exposure, with increased interleukin-4 (IL-4) production, IL-5 transcription, and eosinophilia. Suppression of parasite-specific peripheral blood mononuclear cell (PBMC) proliferation was evident, while decreased mitogen-stimulated gamma interferon (IFN-γ) production suggested immunomodulation, which was not restricted to parasite-specific responses. Our findings show that the global immune response is modulated toward a nonproliferative type 2 state following natural challenge withF. hepatica. This has implications in terms of the timing of the administration of vaccination programs and for host susceptibility to coinfecting pathogens.

THE IMPORTANCE OF THE FAMILY FOR GENEALOGICAL STRUCTURE OF VOLYN BEEF OF CATTLE

2016 ◽  
Vol 52 ◽  
pp. 82-94 ◽  
Author(s):  
A. Ye. Pochukalin
Keyword(s):  
Beef Cattle ◽  
Live Weight ◽  
Breeding Value ◽  
Farm Families ◽  
Cattle Breeding ◽  
Reproductive Ability ◽  
The Family ◽  
Minimum Number ◽  
Related Group ◽  
Economic Use

One of the ways of increasing level of animal economically useful traits is selection work with farm families. In pedigree cattle breeding of Ukraine families are a statistical component of breed genealogy. Among the main scientific works on working with families, it should be noted minimum number of female ancestors, proposed by D. T. Vinnichuk, to determine the breeding value, different categories, classification and techniques for evaluating related groups of females. The aim of our research was to analyse importance of farm families for genealogical structure of the breed. The research was on basis of data of primary breeding records at the herd of Volyn Beef cattle of “Zorya” breeding farm, Kovel district, Volyn region. Akula 102, Galka 37 and Galka 1537 families belonging to Krasavchyk 3004 bloodline, Smorodyna 613, Korona 2382 and Visla 1016 families – Tsebryk 3888 bloodline, Kalyna 212, Verba 1536 and Garna 536 families – Yamb 3066 bloodlines, Kazka 433, Galka 421 and Bystra 1124 families – Buinyi 3042 bloodline, Rozetka 1313, Arfa 599 and Bulana 943 families – Sonnyi-Kaktus 3307-9828 bloodline, and Palma 275, Desna 870 and Veselka 444 families – Mudryi 9100 bloodline were characterized. Belonging to a bloodline was determined by the father's side of female ancestors. Structural units of families: branches, branching with identifying the best individuals on breeding traits were submitted to identify the best combinations and successful use of closely related breeding. Comparing assessment of related groups of females on the main breeding traits belonging to Krasavchyk 3004 bloodline, it was noted that the cows of Akula 102 family predominated in live weight at 5 years’ age, milk ability and economic use duration, whereas the cows of Galka 1537 family – on traits of reproductive ability. Smorodyna 613 family of Tsebryk 3888 bloodline had high duration of economic use and cows’ live weight at 5 years’ age compared with Korana 2382 and Visla 1016 families with equal values of the exterior traits (height measures) and coefficient of reproductive ability. The families of Mudryi 9100 bloodline in terms of reproduction (calving interval, coefficient of reproductive ability) had the highest figures of cows’ milk ability and live weight. The cows of Bulana 943 family had a considerable predominance over representatives of Rozetka 1313 and Arfa 599 families of Sonnyi-Kaktus 3307-9828 bloodline by main economically useful traits. High indices of reproductive ability were noted in these families. Heifers of the families of Buinyi 3042 bloodline had high live weight at 18 months’ age at average values of milk ability and cows’ live weight at 5 years’ age. More equal figures of growth rate, exterior and economic use duration were observed in the cows of Kalyna 212, Verba 1536 and Garna 536 families of Yamb 3066 bloodline. Breeding by families in beef cattle breeding is an important element of selection, because it allows to evaluate not only related group of female ancestor, but also to analyse a successful combination with lines and purposeful use of closely related breeding by the best representatives of a breed.

Innate immunity alterations in Type 2 Diabetes Mellitus: understanding infection susceptibility

2020 ◽  
Vol 20 ◽  
Author(s):  
Ochoa-González Fátima de Lourdes ◽  
González-Curiel Irma Elizabeth ◽  
Cervantes-Villagrana Al-berto Rafael ◽  
Fernández-Ruiz Julio Cesar ◽  
Castañeda-Delgado Julio Enrique
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Immune Response ◽  
Type 2 Diabetes Mellitus ◽  
Innate Immune Response ◽  
Adult Population ◽  
International Diabetes Federation ◽  
Innate Immune ◽  
Cellular Mechanisms

: Diabetes is a chronic disease characterized by marked alterations in the metabolism of glucose andby high con-centrations of glucose in the blood due to a decreased insulin production or resistance to the action of this hormone in pe-ripheral tissues. The International Diabetes Federation estimates a global incidence of diabetes of about 10% in the adult population (20 -79 years old), some 430 million cases reported worldwide in 2018. It is well documented that people with diabetes have a higher susceptibility to infectious diseases and therefore show higher morbidity and mortality compared to the non-diabetic population. Given that the innate immune response plays a fundamental role in protecting against invading pathogens through a myriad of humoral and cellular mechanisms, the present work makes a comprehensive review of the innate immune alterations in patients with type 2 diabetes mellitus (T2D) as well as a brief description of the molecular events leading or associated to such conditions.We show that in these patients a compromised innate immune response in-creases susceptibility to infections.

scholarly journals Evaluation of pear (Pyrus communis L.) hybrid combinations for the transmission of fire blight resistance and fruit characteristics

2018 ◽  
Vol 54 (No. 2) ◽  
pp. 78-85 ◽  
Author(s):  
Y. Evrenosoğlu ◽  
K. Mertoğlu
Keyword(s):  
Fire Blight ◽  
Animal Health ◽  
Blight Resistance ◽  
Fruit Characteristics ◽  
Breeding Programs ◽  
Pyrus Communis L ◽  
Management Method ◽  
Resistant Rootstock ◽  
Santa Maria ◽  
Harmful Effects

Fire blight is one of the most destructive diseases of pome fruits, especially pears. In current conditions when the demand for organic products is increasing, improvement of resistant rootstock and varieties is becoming important due to the lack of an effective disease management method against fire blight caused by Erwinia amy-lovora as well as the harmful effects of chemicals on environment, human and animal health. The findings of the researchers as to which variety performs well in terms of the transmission of fire blight resistance are quite important for new breeding programs. In the study, three varieties with high commercial value (Magness, Santa Maria and Williams) were identified as the maternal parents and 21 hybridization combinations were made with seven varieties (Akça, Ankara, Conference, Güz, Kaiser Alexandre, Kieffer and Taş) as pollinators. The Magness maternal parent was found superior when compared with the other maternal parents for the variety susceptibility level to fire blight. Apart from the differences in the susceptibility level of 21 different combinations of hybrids to fire blight, the commercially superior ten hybrids (I-15-24, II-13-1, II-13-19, II-13-34, II-13-73, II-27-21, II-27-55, II -32-44, III-22-638, III-27-590) have been determined via the weighted ranking method from the parameters of disease resistance and different fruit characteristics measuring the commercial preference of the fruits such as fruit quality, attractiveness, size, firmness and rustiness

scholarly journals Maintenance of Type 2 Response by CXCR6-Deficient ILC2 in Papain-Induced Lung Inflammation

2019 ◽  
Vol 20 (21) ◽  
pp. 5493 ◽  
Author(s):  
Meunier ◽  
Chea ◽  
Garrido ◽  
Perchet ◽  
Petit ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Nk Cell ◽  
Lymphoid Cells ◽  
Inflammatory Conditions ◽  
Airway Diseases ◽  
Type 2 Cytokines ◽  
Lymphoid Organogenesis ◽  
Deficient Mice

Innate lymphoid cells (ILC) are important players of early immune defenses in situations like lymphoid organogenesis or in case of immune response to inflammation, infection and cancer. Th1 and Th2 antagonism is crucial for the regulation of immune responses, however mechanisms are still unclear for ILC functions. ILC2 and NK cells were reported to be both involved in allergic airway diseases and were shown to be able to interplay in the regulation of the immune response. CXCR6 is a common chemokine receptor expressed by all ILC, and its deficiency affects ILC2 and ILC1/NK cell numbers and functions in lungs in both steady-state and inflammatory conditions. We determined that the absence of a specific ILC2 KLRG1+ST2– subset in CXCR6-deficient mice is probably dependent on CXCR6 for its recruitment to the lung under inflammation. We show that despite their decreased numbers, lung CXCR6-deficient ILC2 are even more activated cells producing large amount of type 2 cytokines that could drive eosinophilia. This is strongly associated to the decrease of the lung Th1 response in CXCR6-deficient mice.

scholarly journals Maternally Derived Antibody Levels Influence on Vaccine Protection against PCV2d Challenge

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2231
Author(s):  
István Kiss ◽  
Krisztina Szigeti ◽  
Zalán G. Homonnay ◽  
Vivien Tamás ◽  
Han Smits ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Subunit Vaccine ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Vaccine Protection ◽  
Humoral Immune ◽  
Negative Effect ◽  
Antibody Levels

Piglets from a porcine circovirus type 2 (PCV2) stable farm of low and high levels of maternally derived antibodies (MDA) against PCV2 were vaccinated either with a whole virus type or a PCV2 ORF2 antigen-based commercial subunit vaccine at three weeks of age. Two non-vaccinated groups served as low and high MDA positive controls. At four weeks post vaccination, all piglets were challenged with a PCV2d-2 type virus strain and were checked for parameters related to vaccine protection over a four-week observation period. MDA levels evidently impacted the outcome of the PCV2d-2 challenge in non-vaccinated animals, while it did not have a significant effect on vaccine-induced protection levels. The humoral immune response developed faster in the whole virus vaccinates than in the subunit vaccinated pigs in the low MDA groups. Further, high MDA levels elicited a stronger negative effect on the vaccine-induced humoral immune response for the subunit vaccine than for the whole virus vaccine. The group-based oral fluid samples and the group mean viraemia and faecal shedding data correlated well, enabling this simple, and animal welfare-friendly sampling method for the evaluation of the PCV2 viral load status of these nursery piglets.

scholarly journals Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1005
Author(s):  
Guoxiu Li ◽  
Ashenafi Kiros Wubshet ◽  
Yaozhong Ding ◽  
Qian Li ◽  
Junfei Dai ◽  
...  
Keyword(s):  
South African ◽  
Recombinant Proteins ◽  
Fusion Proteins ◽  
Serum Antibody ◽  
Vaccine Design ◽  
Structural Proteins ◽  
The South ◽  
Study Results ◽  
Mouth Disease Virus

An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SAT2 are poorly understood. Similarly, the particular roles of the three structural proteins in novel vaccine design and development remain unexplained. We therefore constructed VP1, VP0, and VP3 encoding gene (SAT2:JX014256 strain) separately fused with His-SUMO (histidine-small ubiquitin-related modifier) inserted into pET-32a cassette to express the three recombinant proteins and separately evaluated their antigenicity and immunogenicity in mice. The fusion protein was successfully expressed and purified by the Ni-NTA resin chromatography. The level of serum antibody, spleen lymphocyte proliferation, and cytokines against the three distinct recombinant proteins were analyzed. Results showed that the anti-FMDV humoral response was triggered by these proteins, and the fusion proteins did enhance the splenocyte immune response in the separately immunized mice. We observed low variations among the three fusion proteins in terms of the antibody and cytokine production in mice. Hence, in this study, results demonstrated that the structural proteins of SAT2 FMDV could be used for the development of immunodiagnostic kits and subunit vaccine designs.

scholarly journals Response of Pea Varieties to Damage Degree of Pea Weevil,Bruchus pisorumL.

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Ivelina Mitkova Nikolova
Keyword(s):  
Crop Yields ◽  
Egg Laying ◽  
Breeding Programs ◽  
Good Opportunity ◽  
Damage Degree ◽  
Field Germination ◽  
Type 3 ◽  
Emergence Hole

A study was conducted to determine the response of five pea varieties (Pisum sativumL.) to damage degree ofBruchus pisorum: Glyans, Modus, Kamerton, and Svit (Ukrainian cultivars) and Pleven 4 (Bulgarian cultivar). The seeds were classified into three types: healthy seeds (type 1), damaged seeds with parasitoid emergence hole (type 2), and damaged seeds with bruchid emergence hole (type 3) and they were sown. It was found that the weight of 1000 seeds did not affect the field germination of the pea varieties. Healthy and damaged seeds with parasitoid emergence holes (first and second seed types) provide a very good opportunity for growth and development while plants from damaged seeds with bruchid emergence holes had poor germination and vigor and low productivity. These seeds cannot provide the creation of well-garnished seeding and stable crop yields. Among tested varieties, the Ukrainian variety Glyans had considerably higher seed weight, field germination, and index germination and weak egg-laying activity ofB. pisorumcompared to others. Use of spring pea cultivars that are weakly preferred by the pea weevil in breeding programs would reduce losses due to pea weevil and provide an environmentally safer option to its control.

Su.77. Immune Response to T-independent Antigen Type 2 in vitro

2008 ◽  
Vol 127 ◽  
pp. S149
Author(s):  
Ekaterina Sidorova ◽  
Marina Gavrilova ◽  
Irina Chernyshova
Keyword(s):  
Immune Response
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